FastQCFastQC Report
Fri 17 Jun 2016
SRR1525869_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525869_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688
Sequences flagged as poor quality0
Sequence length40
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT60.35545023696682465No Hit
ATGGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT40.23696682464454977No Hit
ATGGCGGGGAAAAAAAAACAAAAAAAAAAAAAAAAAAAAA30.17772511848341233No Hit
ATGGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT30.17772511848341233No Hit
ATGGCGGGGATCCCGTGACAGCTACCATTAGCTTCCAGAG20.11848341232227488No Hit
ATGGCGGGGACCTTTATGTTACTTTATGCCGCCGGCTCTT20.11848341232227488No Hit
ATGGCGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT20.11848341232227488No Hit
ATGGCGGGGTCGAGTCTTTTTTTTTTTTTTTTTTTTTTTT20.11848341232227488No Hit
ATGGCGGGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTT20.11848341232227488No Hit
ATGGCGGGGGTCGATTTTTTTTTTTTTTTTTTTTTTTTTT20.11848341232227488No Hit
ATGGCGGGGGCCGAATAGCCCCTCCCCCCCACCCGCCCGC20.11848341232227488No Hit
ATGGCGGATGGTACGAAGTCGTTTTTTTTTTTTTTTTTTT20.11848341232227488No Hit
ATGGCGGGGATGAGATGGACTTAAATGAAATGACCGGTAC20.11848341232227488No Hit
ATGGCGGAAGGACATGGACACTGTCTATCTCTGCTCTGTT20.11848341232227488No Hit
ATGGCGGGGTGTACGCCGAGTCGATTTTTTTTTTTTTTTT20.11848341232227488No Hit
ATGGCGGGGTTTCGATTTTTTTTTTTTTTTTTTTTTTTTT20.11848341232227488No Hit
ATGGCGGGGGTCGACCTTTTTTTTTTTTTTTTTTTTTTTT20.11848341232227488No Hit
ATGGCGGGGTACAACGCAGAGTCGACTTTTTTTTTTTTTT20.11848341232227488No Hit
ATGGCGGGGTGTACGCAGCGTCCACTTTTTTTTTTTTTTT20.11848341232227488No Hit
ATGGCGGGGTGTACGCAGCGTCTTTTTTTTTTTTTTTTTT20.11848341232227488No Hit
ATGGCGGGGTACAGAGCTTTTTTTTTTTTTTTTTTTTTTT20.11848341232227488No Hit
ATGGCGGGGTGGTCGCAGCGCGACTTTTTTTTTTTTTTTT20.11848341232227488No Hit
ATGGCGGGAAGCAGGTATCAACGCAGAGTCTCTTTTTTTT20.11848341232227488No Hit
ATGGCGGGGTACGCCGAGTCGACTTTTTTTTTTTTTTTTT20.11848341232227488No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGC251.3657115E-433.6363644
GCGGGGA354.9449227E-733.6363644
CGGGGGG251.3657115E-433.6363645
CGGGGAA251.3657115E-433.6363645
GGGGAAG251.3657115E-433.6363646
GCGGGGT308.343952E-633.636364
GGGAAGC200.002172397233.636367
ATGGCGG1650.032.617081
TGGCGGG1650.032.617082
GGCGGGG1550.032.551323
GCGGGGG650.031.048954
TTTTTTT1900.00162411917.96650734
Produced by FastQC (version 0.11.5)