FastQCFastQC Report
Fri 17 Jun 2016
SRR1525867_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525867_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64
Sequences flagged as poor quality0
Sequence length40
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.9375No Hit
ATGGCGGGGGGGAAATAGTGGAAAAAAAAAAGGCTCGGTT11.5625No Hit
ATGGCGGGGTAAAAAATCATCTTCTGAAGGAGATTGGGCG11.5625No Hit
ATGGCGGGGTCACAGAAACTCTTCTGAAAGAGATTTGGCG11.5625No Hit
ATGGCGGAGAAGCAGTGCTATCAACTCAGAGTCGACTTTT11.5625No Hit
ATGGCGGGGGGCGGGGGGGAGGTGCCTGCTGTTTATAATG11.5625No Hit
ATGGCGGGGTCAAAGATCTTCTCCTGAAGGAGATTGGGAG11.5625No Hit
ATGGCGGGGTCACAGATACTCTGAAAGAAATTGGGCGGAT11.5625No Hit
ATGGCGGGGTCACAGGTTCCCAGTCTTCAAGGGTCGTGTA11.5625No Hit
ATGGCGGGGGAGTCGAATTTTTTTTTTTTTTTTTTCTTTT11.5625No Hit
ATGGCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTTT11.5625No Hit
ATGGCGGGGGGTTGCCTCTGGGTTGTACAAGCTGAAGAGC11.5625No Hit
ATGGCGGGGGAGTCGAATTTTTTTTTTTTTTTTTTTTTTT11.5625No Hit
ATGGCGGGGAATCTTTGGGGAAAATTTAAAATAAATAATT11.5625No Hit
ATGGCGGGGGGGAAATCGTGGAGAAAGAAAAAGATTCTAT11.5625No Hit
ATGGCGCTCGCCGTTCGAGTCGTGTATTCTTGATCTTTAG11.5625No Hit
ATGGCGGGGTCAAAGATCCTCTTCAGAGATGAGTTTTTGT11.5625No Hit
ATGGCGGGGGGGAAAAAAAAAAAAAAAAAAACAAATAAAA11.5625No Hit
ATGGCGGGGTCACAGATCCTCTTATGAGAAGAGTTTTTGT11.5625No Hit
ATGGCGGGGTCAAAAAAACTCCTCTGAGATGAGTTTTTGT11.5625No Hit
ATGGCGGGGTCAAAGATCCTCTTCTTAGATGAGTTTTTGT11.5625No Hit
ATGGCGGGGGTTTACGCTGATGACCGACTGGATGCCCTGT11.5625No Hit
ATGGCGGGCTCAAAAAAACTCTTCTGAAAGAGATTTGGCG11.5625No Hit
ATGGCGGGGTCAAAAAACCTCTTCTGAGATAAGTTTTTAT11.5625No Hit
ATGGCGGGGTCACAAAAACTCTTCTGAGAAGAGTTTTTTT11.5625No Hit
ATGGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGT11.5625No Hit
ATGGCGGGGTCACAAAACCTCTTCCGAAAAGAAATTTTTT11.5625No Hit
ATGGCGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.5625No Hit
ATGGCGGGGATTATTTTTTGAAAGAAATTGGGCGGATTTA11.5625No Hit
ATGGCGGGGTGGCAAAAAAAACAAAAAAAAAAAAAAAAAA11.5625No Hit
ATGGCGGGGGGGAATTAGTGGAGAAAGAAATGGCTCGTTT11.5625No Hit
ATGGCGGGGAACAAAAACAAAAAAAAAAAAAAAAAAAAAA11.5625No Hit
ATGGCGGATAAAAAAGAAAAACGAACAGAGTAAAACAAAA11.5625No Hit
ATGGCGGGGGAAAAGGAAAAGAGTAAGTAAGGCCAGTCCC11.5625No Hit
ATGGCGGGGGTGCAGTGCTAAGGCCGGGGCAAAAATACTC11.5625No Hit
ATGGCGGGGTCACTCCTCTTCTGAGATGAGTTTTTGTTCA11.5625No Hit
ATGGCGGGGTCCAAGAACCTCCTCTTAGATGATTTTTTGT11.5625No Hit
ATGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAACAAA11.5625No Hit
ATGGCGGGGAAAAAGTGGTAACAAAGCAAAATCGAAAAAA11.5625No Hit
ATGGCGGGGCTCCGAAAAACTCGTAAGCCGTCTTCTTCTT11.5625No Hit
ATGGCGGGGAAAAAAAACCTCTTCTGAAGGAGATTTGGCG11.5625No Hit
ATGGCGGGGGAGGAAGAAGGAGAGGAATACTAAATTAAAT11.5625No Hit
ATGGCGGGGTCACAGAACCTCTTCTGAAGGAGATTGGGCG11.5625No Hit
ATGGCGGGGTAAAAAAAACTATTCTGAGATGAGTTTTTGT11.5625No Hit
ATGGCGGGGAAAAAACAAAAAAAAAAAAAAAAAAAAAAAA11.5625No Hit
ATGGCGGGGTGGGTTGTTGCAGGCATTTGTCTATAGCGGT11.5625No Hit
ATGGCGGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAA11.5625No Hit
ATGGCGGGGGAGTGCAGCGCTATGGCGGGAAAAAAAAAAA11.5625No Hit
ATGGCGGGGGGGCAAAAAAGAGAAAACNAATCTTAAATAA11.5625No Hit
ATGGCGGGGGGGAGGTAGTGAACAAAAATAAAAAAAAAAA11.5625No Hit
ATGGCGGGGTCAAAGATACCCTTCCGAGATGAGTTTTTTT11.5625No Hit
ATGGCGGGGTGTCCTCTTCTGAGATGAGTTTTTGTTCAGA11.5625No Hit
ATGGCGGGGAAAAATTTCAAAAAGGAGACTGCGCATGCAG11.5625No Hit
ATGGCGGGGTGAAAAAAAATAAAAAAGAGAGAAAGAATTG11.5625No Hit
ATGGCGGGGGGAAATTGTGGAGAAAGAAAAGGCTCGTTTT11.5625No Hit
ATGGCGGGGTCAAAGATCATCTTCTGAGATGAGTTTAATT11.5625No Hit
ATGGCGGGGGAATTAATCCAAGAATAGTGCAAGATATTTT11.5625No Hit
ATGGCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCG11.5625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGT50.034.04
GAGAAAG50.034.029
GGTGAAA50.034.08
CGGGGTG50.034.05
TAAAAAA50.034.020
AAGAGAG50.034.025
GAAAAAA50.034.011
ATGGCGG50.034.01
AAATAAA50.034.017
AGAATTG50.034.034
AAAAGAG50.034.023
AGAAAGA50.034.030
ATAAAAA50.034.019
AAGAATT50.034.033
AGAGAGA50.034.026
AGAGAAA50.034.028
AAAATAA50.034.016
GAGAGAA50.034.027
TGGCGGG50.034.02
GGGGTGA50.034.06