FastQCFastQC Report
Fri 17 Jun 2016
SRR1525866_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525866_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20418
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT6373.1197962582035457No Hit
CCGACGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC4482.1941424233519444No Hit
CCGACGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC1570.7689293760407483No Hit
CCGACGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT1350.6611813106082869No Hit
CCGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC1060.5191497698109511No Hit
CCGACGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC700.34283475364874133No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC660.32324419629738466No Hit
CCGACGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT480.23508668821627976No Hit
CCGACGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC460.22529140954060142No Hit
CCGACGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGAGCTCGTATGCCGT400.19590557351356644Illumina Single End Adapter 2 (95% over 24bp)
CCGACGGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC360.17631501616220982No Hit
CCGACGGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC360.17631501616220982No Hit
CCGACGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA310.15182681947301402No Hit
CCGACGGGGTGCTCCGACGGGGTGCTCATTCTGGTTCACCCTGCCCCTGCCCCCGC310.15182681947301402No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC290.14203154079733568No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC270.13223626212165734No Hit
CCGACGGGGACAGAAAAATGACTTTAGTTATGTAATATGCTTAATTCTCATTCTCT240.11754334410813988No Hit
CCGACGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGACGAGCTCGTATGCCGT240.11754334410813988Illumina Single End Adapter 1 (95% over 23bp)
CCGACGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGATATCGTATGCCGT230.11264570477030071No Hit
CCGACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCA230.11264570477030071No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATT251.9203446E-550.40694838
CGCATTG251.9203446E-550.40694839
GCATTGA251.9203446E-550.40694840
GCTCAAT150.00804996150.40694839
TGTTCCC203.9258267E-450.40694438
TAATCCG150.00812950250.28217742
CCCTCCG203.9743714E-450.28217742
AGAGCCC150.00820962650.15802436
GGGCGCA251.9777739E-550.15802436
TTTGATC204.023392E-450.15802436
CAGTGGT252.0070178E-550.03448513
CTGCGGG150.00829033650.0344849
CAAATCC150.00829033650.0344813
CGGGGCA650.049.789225
GGCACCC150.00845352649.7892158
CGGGGTG301.0271906E-649.7892155
CGGGGGA1950.049.7892155
CGGGGCT600.049.7892155
CGGGGCC204.1733432E-449.7892155
CGGGGAC600.049.7892155
Produced by FastQC (version 0.11.5)