##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525866_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20418
Sequences flagged as poor quality	0
Sequence length	56
%GC	55
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.096336565775296	39.0	38.0	40.0	33.0	40.0
2	36.618620824762466	39.0	37.0	40.0	30.0	40.0
3	35.57782348907826	38.0	35.0	39.0	27.0	40.0
4	34.97355274757567	38.0	36.0	39.0	20.0	40.0
5	35.88642374375551	38.0	35.0	39.0	28.0	40.0
6	35.63483201097071	38.0	35.0	39.0	27.0	40.0
7	36.30654324615535	38.0	36.0	39.0	30.0	40.0
8	36.0768439612107	38.0	36.0	39.0	30.0	40.0
9	36.139043980801254	38.0	36.0	39.0	30.0	40.0
10	35.121608384758545	38.0	35.0	39.0	26.0	40.0
11	34.75531393868155	38.0	34.0	39.0	26.0	40.0
12	33.930698403369576	38.0	33.0	39.0	22.0	40.0
13	33.21103927906749	37.0	32.0	39.0	19.0	40.0
14	34.142913115878144	38.0	33.0	39.0	23.0	40.0
15	32.345724360858064	36.0	30.0	39.0	16.0	40.0
16	32.56744049368204	36.0	30.0	39.0	19.0	40.0
17	31.256636301302773	35.0	27.0	38.0	15.0	40.0
18	31.00519149769811	35.0	26.0	39.0	14.0	40.0
19	32.26035850719953	36.0	29.0	39.0	18.0	40.0
20	32.57155451072583	36.0	30.0	39.0	20.0	40.0
21	33.349005779214416	38.0	32.0	39.0	20.0	40.0
22	32.588500342834756	37.0	30.0	39.0	17.0	40.0
23	33.379958859829564	38.0	32.0	39.0	20.0	40.0
24	32.56704868253502	37.0	30.0	39.0	17.0	40.0
25	33.074199235968265	37.0	31.0	39.0	19.0	40.0
26	33.145508864727205	37.0	32.0	39.0	19.0	40.0
27	33.39827603095308	38.0	33.0	39.0	20.0	40.0
28	33.519443628171224	38.0	33.0	39.0	21.0	40.0
29	32.99240865902635	37.0	32.0	39.0	19.0	40.0
30	32.76937016358115	37.0	31.0	39.0	18.0	40.0
31	33.393623273582136	37.0	33.0	39.0	20.0	40.0
32	33.33210892349887	37.0	33.0	39.0	20.0	40.0
33	33.05563718287785	37.0	33.0	39.0	19.0	40.0
34	32.62885689097855	36.0	32.0	39.0	17.0	40.0
35	32.962386129885395	37.0	32.0	39.0	18.0	40.0
36	31.989763933783916	36.0	31.0	38.0	15.0	40.0
37	31.754726221961015	36.0	30.0	38.0	11.0	40.0
38	31.27529630717994	35.0	30.0	38.0	4.0	40.0
39	29.91654422568322	35.0	27.0	38.0	4.0	40.0
40	27.426731315505926	33.0	18.0	38.0	4.0	39.0
41	25.331374277598197	30.0	12.0	36.0	4.0	39.0
42	26.190518170241944	31.0	18.0	36.0	4.0	39.0
43	26.513272602605543	32.0	18.0	37.0	4.0	39.0
44	25.938632579096875	31.0	16.0	36.0	4.0	39.0
45	26.569497502203937	32.0	19.0	37.0	4.0	39.0
46	24.596679400528945	29.0	13.0	35.0	4.0	39.0
47	23.70491722989519	28.0	9.0	35.0	4.0	39.0
48	24.170731707317074	28.0	11.0	35.0	4.0	39.0
49	23.343030659222254	28.0	6.0	35.0	4.0	39.0
50	23.966842981682827	29.0	4.0	35.0	4.0	39.0
51	24.261289058673718	30.0	4.0	35.0	4.0	39.0
52	24.05230678812812	30.0	4.0	35.0	4.0	39.0
53	23.922470369282006	30.0	4.0	35.0	4.0	39.0
54	23.736653932804387	30.0	4.0	35.0	4.0	39.0
55	23.646880203741798	30.0	4.0	36.0	4.0	39.0
56	22.714761484964246	29.0	4.0	36.0	4.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	2.263522166786089
1	2	3.113811805748824
1	3	3.3550754113946155
1	4	4.872356455004891
1	5	3.9194176355952095
1	6	3.995841079314438
1	7	3.4020677265506905
1	8	3.321833169244485
1	9	3.1482223975241226
1	10	4.685621775835905
1	11	-0.09522028799277393
1	12	-6.9143380101281195
1	13	-16.63450745203967
1	14	-4.819100288360392
1	15	0.7884056290981327
1	16	-3.1153634054636328
1	17	-5.684207447741851
1	18	-3.4607153059652873
1	19	0.24081722855312293
1	20	-0.6837605724413827
1	21	6.008609940658744
1	22	6.81639293207763
1	23	5.525621377339913
1	24	5.560665568020543
1	25	5.233584274233067
1	26	4.440480533814334
1	27	2.4491794168576604
1	28	3.7409772020585166
1	29	5.186722919648986
1	30	4.9609417722360725
1	31	4.855449694671059
1	32	4.128268073574542
1	33	3.519943149217603
1	34	4.265480615407469
1	35	4.701287168295504
1	36	3.7425807222902776
1	37	4.366157592028877
1	38	4.301459139105873
1	39	3.5512690345943803
1	40	-12.51886163711422
1	41	-2.4939229750646206
1	42	-4.654455443609855
1	43	-9.429796814505554
1	44	-14.725262309335221
1	45	-4.684446326173116
1	46	-7.6599031624502185
1	47	-12.331702844183534
1	48	-6.907233437886518
1	49	-12.614459798247786
1	50	-5.699612840314032
1	51	0.23187976541097655
1	52	0.17338604803345703
1	53	-0.346601178010129
1	54	0.4798234570240645
1	55	-0.6768247286630356
1	56	2.685165493438369
2	1	3.130188833452756
2	2	3.75825625019327
2	3	4.77729763361684
2	4	5.527912010560442
2	5	4.300899117076689
2	6	4.1810262644996286
2	7	4.016882541365504
2	8	3.9403516877630054
2	9	3.248222397524124
2	10	4.996732886947015
2	11	4.149224156451673
2	12	0.23010643431632616
2	13	1.4321592146269992
2	14	4.6883071190470105
2	15	6.34396118465369
2	16	5.258710668610444
2	17	7.567644404110002
2	18	3.9096550644050794
2	19	6.685261672997566
2	20	5.753276464595654
2	21	7.178980311029115
2	22	7.93491145059615
2	23	6.8811769328954675
2	24	6.86066556802054
2	25	5.7372879779367665
2	26	5.158999052332849
2	27	5.830660898339147
2	28	6.352088313169624
2	29	5.216352549278611
2	30	6.764645475939776
2	31	4.066560805782174
2	32	4.6541939995004675
2	33	2.879202408476857
2	34	5.161776911703761
2	35	4.338324205332537
2	36	6.557395537105087
2	37	5.206898332769619
2	38	6.723681361328097
2	39	6.68089866422401
2	40	6.607064288811706
2	41	9.354225173083528
2	42	8.815914926760513
2	43	8.585018000309258
2	44	-5.214151198224112
2	45	6.904442562715772
2	46	4.084541281994227
2	47	2.5645934521127636
2	48	4.944618413965333
2	49	-4.399644983432971
2	50	4.070757530056337
2	51	5.813361246892459
2	52	6.5696823443297525
2	53	6.427472896063943
2	54	6.931675308875917
2	55	6.923175271336966
2	56	9.637017345290221
3	1	3.1360711863939272
3	2	3.764138603134441
3	3	3.9988662610678176
3	4	5.202421814482008
3	5	3.8420755876649224
3	6	4.759457637048648
3	7	3.5600197962674685
3	8	3.8501556093316367
3	9	3.7952812210535356
3	10	4.25751720067251
3	11	4.829616313314418
3	12	5.853635846081026
3	13	6.100786665607387
3	14	5.490267903360738
3	15	1.5694513807321222
3	16	7.086161649002602
3	17	8.628428717835494
3	18	8.772400162444299
3	19	7.39114402593874
3	20	7.2650411704780105
3	21	7.153490114950678
3	22	8.021185960400071
3	23	6.894902423091548
3	24	7.472430273902898
3	25	6.584346801466182
3	26	6.008018660175988
3	27	5.969876584613651
3	28	5.4501275288559015
3	29	5.786940784572728
3	30	6.997978809273111
3	31	5.378325511664528
3	32	5.642429293618111
3	33	5.651751428084701
3	34	5.924522009742979
3	35	4.72852028376391
3	36	6.537787693967836
3	37	6.136310097475501
3	38	7.886426459367314
3	39	8.504428075988713
3	40	9.828632916262684
3	41	13.634617329946273
3	42	12.676699240486009
3	43	12.69874349050534
3	44	12.591731154717063
3	45	12.763266092127541
3	46	14.019835399641284
3	47	12.895966001132372
3	48	13.905402727690827
3	49	12.749374624410166
3	50	12.758992824173983
3	51	12.497674972382654
3	52	12.155956854133674
3	53	12.139237601946299
3	54	13.233636093189642
3	55	13.607488996827161
3	56	13.017409502152969
4	1	3.101617404881324
4	2	3.415399107336121
4	3	4.0820595383787435
4	4	4.932673915322347
4	5	3.629470545648118
4	6	4.361978645452005
4	7	2.7121206366036006
4	8	3.630827878239195
4	9	3.919650968952695
4	10	4.549113839327973
4	11	4.768271775499294
4	12	4.84439214860204
4	13	4.508349690817475
4	14	5.08354521428511
4	15	5.810627851320351
4	16	6.463472573372346
4	17	7.748596785062379
4	18	8.70013125488127
4	19	7.199547387283278
4	20	6.924705036024225
4	21	6.169456501505302
4	22	6.81110192678663
4	23	6.319272170990704
4	24	7.274951282306258
4	25	7.10871654936534
4	26	4.454237147570947
4	27	3.9497085173867603
4	28	5.242564503645816
4	29	4.78778112070718
4	30	5.597978809273112
4	31	4.137989377210744
4	32	3.5970511423576106
4	33	3.8744405037149576
4	34	4.43796738789424
4	35	4.7764194434277805
4	36	5.20501458472414
4	37	4.9068983327696145
4	38	5.885586123232862
4	39	6.666612949938298
4	40	-0.6024595207121024
4	41	11.044701363559717
4	42	9.325438736284326
4	43	9.537398952690214
4	44	10.528705944633035
4	45	8.404442562715776
4	46	11.613112710565655
4	47	11.88840297592229
4	48	12.239856509203427
4	49	11.038450254662266
4	50	10.213614672913483
4	51	9.322885056416268
4	52	10.174444249091657
4	53	9.670330038921087
4	54	10.484056261256868
4	55	9.989841938003632
4	56	10.213207821480697
5	1	2.8125417746292243
5	2	3.499432720781499
5	3	4.26357214342076
5	4	5.320068873305544
5	5	4.5773697053119875
5	6	4.612398813519235
5	7	3.7070786197968744
5	8	3.6148614916845716
5	9	4.00116357399471
5	10	4.75751720067251
5	11	4.888439842726179
5	12	5.471282904904555
5	13	4.7478454891368
5	14	4.9020326092430935
5	15	6.98121608661447
5	16	6.82145576664966
5	17	8.010781659011965
5	18	8.65475310362077
5	19	7.773496967115211
5	20	7.5003352881250684
5	21	7.006431291421272
5	22	7.5800094898118395
5	23	6.894902423091548
5	24	6.972430273902898
5	25	7.054935036760298
5	26	6.243312777823046
5	27	4.999288349319535
5	28	5.7148334112088435
5	29	6.316352549278612
5	30	5.2332729269201685
5	31	5.025384335193941
5	32	4.789488117147524
5	33	3.887045545731759
5	34	3.8656984803312113
5	35	5.05204969552862
5	36	6.478964164556068
5	37	7.195133626887262
5	38	7.592308812308488
5	39	7.975016311282836
5	40	8.505103504497981
5	41	11.575793800534505
5	42	10.73552276989777
5	43	9.845802314034753
5	44	11.26820174295236
5	45	11.704442562715773
5	46	11.9904236349354
5	47	11.925377765838256
5	48	12.405402727690827
5	49	13.425845212645463
5	50	13.05311047123281
5	51	12.90943967826501
5	52	12.038309795310145
5	53	12.374531719593357
5	54	14.086577269660228
5	55	13.107488996827161
5	56	11.89976244332944
6	1	2.7968555001194204
6	2	3.424922916859934
6	3	4.143964300283507
6	4	5.394578677227109
6	5	4.234232450410026
6	6	4.514359597832957
6	7	2.6168825413655057
6	8	3.3070183544296725
6	9	3.614889064190791
6	10	3.330066220280351
6	11	3.1158908231183347
6	12	3.663439767649656
6	13	5.298825881293659
6	14	4.521640452380346
6	15	5.043961184653686
6	16	5.52537733527711
6	17	7.134311070776665
6	18	8.676321731071745
6	19	6.651928339664234
6	20	6.853276464595655
6	21	6.178980311029115
6	22	6.00157811726282
6	23	5.481176932895469
6	24	5.627332234687213
6	25	5.937287977936769
6	26	4.82566571899952
6	27	4.597327565005813
6	28	4.385421646502962
6	29	6.483019215945276
6	30	4.897978809273109
6	31	3.5665608057821743
6	32	4.254193999500469
6	33	3.0458690751435284
6	34	3.295110245037094
6	35	2.9383242053325382
6	36	3.25739553710509
6	37	4.373564999436283
6	38	5.090348027994764
6	39	5.080898664224009
6	40	5.073730955478371
6	41	12.05422517308353
6	42	10.882581593427183
6	43	11.518351333642595
6	44	11.719182135109225
6	45	10.704442562715773
6	46	11.717874615327556
6	47	12.897926785446096
6	48	12.011285080632
6	49	12.567021683233698
6	50	12.70409086338967
6	51	12.480027913559127
6	52	11.836349010996418
6	53	12.260806229397275
6	54	10.431675308875917
6	55	11.723175271336967
6	56	9.737017345290223
7	1	3.0864388334527533
7	2	3.589506250193267
7	3	4.941880966950173
7	4	4.948745343893776
7	5	4.0571491170766905
7	6	4.347692931166293
7	7	3.6335492080321714
7	8	3.6111850210963397
7	9	3.3982223975241226
7	10	3.7758995536136837
7	11	2.8075574897850046
7	12	4.717606434316323
7	13	5.334242547960329
7	14	3.3174737857136805
7	15	5.135627851320354
7	16	4.7607940019437756
7	17	5.490561070776668
7	18	7.055488397738415
7	19	4.404011672997566
7	20	4.947026464595655
7	21	4.462313644362446
7	22	4.9494947839294845
7	23	4.466593599562135
7	24	3.748165568020543
7	25	2.9685379779367693
7	26	2.8756657189995174
7	27	3.138994231672477
7	28	3.1229216465029594
7	29	3.410102549278612
7	30	2.2479788092731106
7	31	2.060310805782173
7	32	1.272943999500466
7	33	2.633369075143527
7	34	1.3638602450370954
7	35	1.9049908719992068
7	36	2.828228870438423
7	37	2.2943983327696174
7	38	3.95076469466143
7	39	4.58714866422401
7	40	4.334147622145039
7	41	9.30005850641686
7	42	9.413831593427183
7	43	7.957934666975927
7	44	8.60459880177589
7	45	6.485692562715773
7	46	8.190791281994226
7	47	8.10001011877943
7	48	7.423785080632001
7	49	7.023271683233695
7	50	7.920757530056338
7	51	6.723777913559125
7	52	6.021765677663087
7	53	6.258722896063944
7	54	6.7795919755425835
7	55	6.833591938003632
7	56	5.745350678623556
8	1	2.865672704420497
8	2	3.4614820566448827
8	3	4.6203083863050125
8	4	5.341890505184097
8	5	3.8847700848186264
8	6	3.912209060198549
8	7	2.4641943693224917
8	8	3.528523730773756
8	9	3.5998353007499304
8	10	5.334367295549164
8	11	4.198686522043069
8	12	4.626880627864708
8	13	5.720331257637746
8	14	5.287231850229809
8	15	7.139660109384874
8	16	6.001721421298615
8	17	7.8020530062605395
8	18	8.43653678483519
8	19	6.3626810278362775
8	20	7.288760335563399
8	21	6.389732999201158
8	22	6.990825429090773
8	23	5.812359728594391
8	24	7.205826858343126
8	25	6.646965397291609
8	26	4.625665718999517
8	27	5.8768974574789254
8	28	3.604776485212639
8	29	5.622804162181836
8	30	4.965720744756982
8	31	4.031076934814429
8	32	4.69935528982305
8	33	4.865223913853207
8	34	4.99188443858548
8	35	3.6307973236121107
8	36	5.7939546768900385
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115	20	-0.14672353540434457
115	21	5.212313644362446
115	22	5.218244783929482
115	23	5.4478435995621375
115	24	5.61066556802054
115	25	3.887287977936772
115	26	4.6756657189995146
115	27	4.21399423167248
115	28	4.135421646502962
115	29	3.4163525492786135
115	30	3.5479788092731077
115	31	4.116560805782171
115	32	5.454193999500465
115	33	3.5458690751435284
115	34	2.795110245037094
115	35	3.204990871999204
115	36	4.790728870438421
115	37	5.106898332769617
115	38	5.907014694661427
115	39	6.880898664224013
115	40	7.190397622145042
115	41	8.98755850641686
115	42	8.582581593427186
115	43	4.951684666975925
115	44	-1.4141511982241113
115	45	8.954442562715773
115	46	-0.7654587180057746
115	47	-10.01873988122057
115	48	1.1612850806320019
115	49	-6.232978316766305
115	50	0.570757530056337
115	51	1.8300279135591246
115	52	3.403015677663088
115	53	1.677472896063943
115	54	1.2983419755425842
115	55	1.2398419380036323
115	56	4.970350678623557
116	1	2.6801888334527533
116	2	2.9749229168599314
116	3	4.202297633616837
116	4	4.927912010560441
116	5	3.6508991170766905
116	6	4.347692931166293
116	7	3.466882541365507
116	8	2.9236850210963397
116	9	3.2732223975241226
116	10	3.8383995536136837
116	11	2.5575574897850046
116	12	-0.053226899017012386
116	13	4.823825881293665
116	14	3.9216404523803448
116	15	-5.406038815346314
116	16	3.20871066861044
116	17	5.167644404110003
116	18	4.284655064405079
116	19	2.8935950063309015
116	20	3.811609797928991
116	21	4.795646977695782
116	22	5.5432447839294845
116	23	3.7061769328954703
116	24	5.1439989013538785
116	25	5.312287977936769
116	26	4.875665718999517
116	27	4.013994231672477
116	28	3.018754979836295
116	29	3.6080192159452764
116	30	5.164645475939775
116	31	4.341560805782173
116	32	3.3458606661671304
116	33	2.945869075143527
116	34	4.06177691170376
116	35	3.904990871999207
116	36	5.682395537105087
116	37	5.481898332769617
116	38	6.148681361328094
116	39	8.30589866422401
116	40	9.532064288811704
116	41	7.529225173083528
116	42	6.340914926760515
116	43	-0.4066486663574054
116	44	-4.822484531557443
116	45	5.579442562715773
116	46	0.5345412819942261
116	47	-7.36873988122057
116	48	-0.0553815860346667
116	49	-3.6329783167663052
116	50	0.3374241967230063
116	51	5.130027913559125
116	52	4.719682344329755
116	53	3.3524728960639436
116	54	4.4983419755425835
116	55	4.9065086046703
116	56	6.453684011956888
117	1	1.6670309387159108
117	2	2.2950983554564246
117	3	3.0685257037922753
117	4	3.919140080735879
117	5	1.9272149065503754
117	6	3.1634824048505052
117	7	2.2519702606637537
117	8	2.594737652675285
117	9	2.0429592396293828
117	10	2.6739258694031562
117	11	-0.475337247057098
117	12	-5.575156723578413
117	13	-8.371349557302828
117	14	-2.323973582707371
117	15	-6.4104247802585945
117	16	-7.1816402085825395
117	17	0.9395742286714039
117	18	-0.4412221285773761
117	19	0.15894588352388084
117	20	0.24801330670091914
117	21	4.343892591730864
117	22	4.826139520771587
117	23	4.8531067574568745
117	24	3.0869813574942278
117	25	5.3583406095157144
117	26	4.257244666367935
117	27	4.47452054746195
117	28	3.869632172818747
117	29	5.1321420229628245
117	30	4.419031440852056
117	31	2.0718239636769127
117	32	3.7384045258162537
117	33	3.024816443564582
117	34	2.684583929247623
117	35	3.404990871999207
117	36	6.101255186227895
117	37	3.4226878064538298
117	38	2.4885936420298513
117	39	4.496688137908222
117	40	3.635134464250303
117	41	1.1454532432589666
117	42	0.014160540795604248
117	43	-5.737789017234601
117	44	-5.508888040329374
117	45	-1.4271363846526484
117	46	-4.215458718005774
117	47	-2.9476872496416213
117	48	-3.1255570246311564
117	49	-4.185609895713675
117	50	-3.2239793120489253
117	51	-1.1462878759145596
117	52	-1.2496159012842831
117	53	-1.9567376302518475
117	54	-1.212184340246889
117	55	-1.5364738514700527
117	56	1.1861401523077646
118	1	2.1933467281895958
118	2	2.8214141449301096
118	3	3.9632625458975426
118	4	4.813876922841146
118	5	3.5061622749714303
118	6	3.216113983797875
118	7	3.041443944874274
118	8	3.2789481789910795
118	9	3.3061171343662252
118	10	4.0423469220347386
118	11	-0.6858635628465777
118	12	-5.627788302525783
118	13	-5.5292442941449345
118	14	-3.6397630563915833
118	15	-10.673582674995437
118	16	-7.497429682266752
118	17	0.3606268602503526
118	18	-5.335958970682636
118	19	-1.630527800686643
118	20	-1.8046182722464508
118	21	1.6596820654150761
118	22	4.457718468140012
118	23	1.6425804416673948
118	24	4.086981357494228
118	25	3.4109721884630844
118	26	2.6256657189995174
118	27	2.316625810619847
118	28	3.501211120187172
118	29	3.1847736019101944
118	30	2.787452493483638
118	31	3.0191923847295428
118	32	4.212088736342569
118	33	2.498500654090897
118	34	3.7372155081949927
118	35	3.773411924630789
118	36	4.364413080964738
118	37	4.0016351748748775
118	38	3.9096462736088
118	39	5.9177407694871675
118	40	8.529871306355567
118	41	7.250716401153703
118	42	7.1720552776377104
118	43	-0.3693679646030219
118	44	1.3332172228285195
118	45	3.888653089031564
118	46	-1.4259850337952464
118	47	0.3681022240425875
118	48	0.2954956069477923
118	49	-0.6066625272926203
118	50	2.1444417405826535
118	51	3.643185808295968
118	52	4.118805151347296
118	53	3.306420264484995
118	54	3.6299209229110048
118	55	4.463526148529947
118	56	6.607192783886713
119	1	2.1801888334527533
119	2	2.9082562501932685
119	3	4.010630966950174
119	4	4.961245343893779
119	5	3.3508991170766933
119	6	4.14769293116629
119	7	3.03354920803217
119	8	3.3236850210963382
119	9	3.6482223975241226
119	10	4.713399553613684
119	11	0.9325574897850046
119	12	-4.769893565683677
119	13	-0.8845074520396707
119	14	-2.4950262142863195
119	15	-7.639372148679644
119	16	-7.657955998056224
119	17	0.6843110707766691
119	18	-2.3570116022615863
119	19	-0.3647383270024349
119	20	-0.246723535404346
119	21	5.0623136443624475
119	22	5.268244783929486
119	23	4.747843599562135
119	24	5.060665568020543
119	25	5.187287977936769
119	26	5.725665718999519
119	27	5.063994231672474
119	28	4.9354216465029594
119	29	6.466352549278611
119	30	6.597978809273112
119	31	4.866560805782171
119	32	5.054193999500466
119	33	5.89586907514353
119	34	5.745110245037097
119	35	6.054990871999205
119	36	7.390728870438423
119	37	8.156898332769615
119	38	8.757014694661429
119	39	9.630898664224013
119	40	12.640397622145038
119	41	10.037558506416858
119	42	8.582581593427186
119	43	-2.498315333024074
119	44	-2.81415119822411
119	45	10.104442562715771
119	46	0.38454128199422755
119	47	1.2312601187794314
119	48	-6.188714919368
119	49	-0.13297831676630523
119	50	3.220757530056339
119	51	10.180027913559126
119	52	10.503015677663086
119	53	9.277472896063944
119	54	8.298341975542584
119	55	10.239841938003632
119	56	12.070350678623559
120	1	1.9301888334527533
120	2	2.505624671245897
120	3	3.5948414932659603
120	4	4.182297975472721
120	5	3.243004380234588
120	6	3.4792718785347105
120	7	1.8309176290848015
120	8	2.594737652675285
120	9	3.0429592396293828
120	10	2.6739258694031562
120	11	0.524662752942902
120	12	-9.206735670946834
120	13	-2.9502969257238796
120	14	-2.9029209511284257
120	15	1.2737857460571966
120	16	-5.286903366477276
120	17	2.09746896551351
120	18	-3.3359589706826362
120	19	0.6852616729975658
120	20	1.1427501488061829
120	21	2.3965241706782336
120	22	5.720876362876854
120	23	5.011001494298977
120	24	4.40277083117844
120	25	3.3583406095157144
120	26	5.5730341400521475
120	27	4.000836336935635
120	28	3.1854216465029594
120	29	4.44793149664703
120	30	5.576926177694165
120	31	4.6507713320979605
120	32	3.633141367921521
120	33	3.0774480225119447
120	34	3.7372155081949927
120	35	4.5628856088413094
120	36	5.890728870438423
120	37	4.685845701190672
120	38	6.278067326240375
120	39	7.18089866422401
120	40	5.266713411618724
120	41	-6.380862546214718
120	42	-1.564786827625447
120	43	-8.527262701445126
120	44	-14.61415119822411
120	45	-7.742925858336857
120	46	-9.741774507479459
120	47	-9.895055670694255
120	48	-7.388714919367999
120	49	-14.290873053608411
120	50	-7.69766352257524
120	51	-2.198919454861926
120	52	-2.9338264276000707
120	53	-2.8514744723571077
120	54	-1.8963948665626802
120	55	-2.3259475356805766
120	56	-3.7085966897974956
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	435.0
5	43.0
6	78.0
7	55.0
8	84.0
9	89.0
10	66.0
11	55.0
12	65.0
13	56.0
14	60.0
15	68.0
16	84.0
17	137.0
18	152.0
19	189.0
20	258.0
21	340.0
22	419.0
23	518.0
24	573.0
25	607.0
26	728.0
27	668.0
28	698.0
29	712.0
30	810.0
31	935.0
32	1069.0
33	1288.0
34	1357.0
35	1388.0
36	1550.0
37	1716.0
38	1679.0
39	1389.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	99.32412577137819	0.577921441865021	0.07346459006758742	0.024488196689195805
8	99.49554314820257	0.3085512782838672	0.13223626212165734	0.0636693113919091
9	95.5088647272015	1.3272602605544128	2.5271818983250074	0.636693113919091
10	41.19404447056519	19.12038397492409	30.247820550494666	9.437751004016064
11	27.27985111176413	19.73748653149182	14.609658144774219	38.37300421196983
12	25.18855911450681	33.543931824860415	17.435596042707417	23.83191301792536
13	18.611029483788812	14.71740621020668	19.375061220491723	47.29650308551278
14	18.5718483690861	41.75727299441669	19.28200607307278	20.388872563424428
15	41.37998917269551	17.717407352724052	19.62202864314189	21.280574831438557
16	21.054695157812194	40.3793373848265	21.128210154871592	17.437757302489707
17	22.062209159931424	17.0364927749204	42.380602498163114	18.52069556698506
18	19.91380154765403	16.269957880301693	18.33676168086982	45.47947889117445
19	19.745160499877482	17.58392550845381	20.308747855917666	42.36216613575104
20	20.46780758103271	18.31020448193007	42.11739322316481	19.10459471387241
21	19.306494269761977	16.568713879909883	22.35772357723577	41.76706827309237
22	18.155549025369773	16.407091781761192	45.87618767753943	19.56117151532961
23	18.948966598099716	18.90488784405916	42.266627485551965	19.879518072289155
24	18.69703649277492	19.833455792309575	20.91599314229733	40.55351457261817
25	20.621081504702193	20.488832288401255	39.782523510971785	19.107562695924766
26	44.66823483289228	21.135940409683425	16.691169263941976	17.50465549348231
27	21.682435413500663	40.89906367959213	16.81945193391833	20.59904897298887
28	31.095112155940836	32.23136448231953	17.36213145263983	19.311391909099815
29	33.164867517173704	30.8243375858685	18.493621197252207	17.517173699705594
30	30.874785591766724	20.372457730948295	31.29135015927469	17.461406518010293
31	34.1971196237876	29.793279122171057	18.766532771627315	17.243068482414028
32	33.91300088174782	31.189379837366516	19.231899676692464	15.665719604193201
33	36.70917116233448	27.773418342324668	19.264345267287887	16.253065228052968
34	21.9833087874325	18.384879725085913	43.56897398134512	16.062837506136475
35	19.433814149739966	22.578745952310864	42.30693749386714	15.680502404082034
36	29.145433163465306	20.937867502940026	33.339866718933756	16.576832614660916
37	32.175448925522524	20.09616328132666	30.752624865077028	16.97576292807379
38	32.87180242549222	20.056954877988904	30.28919330289193	16.782049393626945
39	32.9227598390421	20.30130532927667	19.54068112670527	27.235253704975953
40	25.08036994905782	16.909837281764677	29.111232009496018	28.898560759681484
41	21.53830904945981	15.710179403310537	25.830112003171774	36.921399544057884
42	18.639489194499017	30.785854616895875	21.73870333988212	28.835952848722986
43	16.570024570024568	28.93857493857494	28.98280098280098	25.50859950859951
44	17.735979122556504	28.51445172091191	30.853316263725443	22.896252892806146
45	27.501718550525382	21.074339585583814	30.437984876755376	20.98595698713542
46	17.291236352906463	30.992426477820402	22.322218943641193	29.39411822563195
47	16.79795194958645	26.526191413942495	19.259551004332415	37.41630563213864
48	28.95304381923341	21.174394667189492	22.92912459562788	26.94343691794922
49	17.586088323426832	28.07879353539323	21.447168050302107	32.88795009087783
50	16.67811917149308	31.677628349857663	21.453813684107196	30.19043879454206
51	17.010714636783643	31.082276614567974	23.336282315934337	28.570726432714043
52	18.19653632929402	41.18628268655252	22.288181327576904	18.32899965657656
53	17.695028492827667	38.93692277461191	23.162703871094518	20.205344861465907
54	17.06370646888354	21.474532147944398	33.23837123630827	28.223390146863796
55	17.917853984474796	19.421243981526974	33.49218826766238	29.16871376633586
56	16.304881642274825	20.985168451036245	33.97505156664375	28.734898340045184
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.0
10	1.0
11	1.0
12	2.0
13	15.0
14	27.0
15	28.5
16	30.0
17	53.0
18	76.0
19	78.5
20	81.0
21	69.5
22	62.5
23	67.0
24	69.5
25	72.0
26	65.0
27	58.0
28	62.5
29	78.0
30	89.0
31	94.5
32	100.0
33	109.5
34	119.0
35	153.5
36	188.0
37	197.5
38	256.5
39	306.0
40	313.0
41	320.0
42	315.0
43	310.0
44	359.0
45	408.0
46	475.5
47	753.0
48	963.0
49	1561.0
50	2159.0
51	2275.5
52	2392.0
53	1951.0
54	1341.0
55	1172.0
56	1120.0
57	1068.0
58	1101.5
59	1135.0
60	1084.5
61	1034.0
62	1059.0
63	1056.5
64	1029.0
65	1018.5
66	1008.0
67	903.5
68	799.0
69	711.5
70	624.0
71	536.0
72	402.0
73	356.0
74	281.5
75	207.0
76	164.0
77	121.0
78	109.5
79	68.0
80	38.0
81	31.0
82	24.0
83	19.5
84	15.0
85	9.0
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.484866294446077
16	0.06856695072974826
17	0.014692918013517485
18	0.0
19	0.0636693113919091
20	0.12244098344597903
21	0.0
22	0.0
23	0.0
24	0.014692918013517485
25	0.009795278675678323
26	0.05877167205406994
27	0.09305514741894406
28	0.0
29	0.18611029483788813
30	0.0636693113919091
31	0.019590557351356647
32	0.019590557351356647
33	0.13713390145949653
34	0.23508668821627976
35	0.17631501616220982
36	0.04897639337839161
37	0.17631501616220982
38	0.24977960622979722
39	0.19590557351356644
40	0.9746302282299932
41	1.1754334410813987
42	0.28406308159467136
43	0.333039474973063
44	0.5338426878244686
45	0.2546772455676364
46	0.4114017043784896
47	0.5191497698109511
48	0.07836222940542659
49	0.2987559996081889
50	0.2154961308649231
51	0.35263003232441964
52	0.17141737682437067
53	0.30365363894602804
54	0.2889607209325105
55	0.31344891762170635
56	0.27426780291899305
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	20418.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.12087373885787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.89692084049162	66.6323831913018
2	5.894013479582397	8.737388578705065
3	1.764239460816704	3.9230091096091684
4	0.8061318884630633	2.390047996865511
5	0.5087881591119334	1.8855911450680773
6	0.27091317563102946	1.2048192771084338
7	0.2510902603409541	1.302772063865217
8	0.10572221488040175	0.6268978352434127
9	0.0726840227302762	0.484866294446077
>10	0.38324302894145634	4.882946419825644
>50	0.013215276860050218	0.666078949946126
>100	0.026430553720100437	4.14340287981193
>500	0.006607638430025109	3.1197962582035457
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCGACGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	637	3.1197962582035457	No Hit
CCGACGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	448	2.1941424233519444	No Hit
CCGACGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	157	0.7689293760407483	No Hit
CCGACGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	135	0.6611813106082869	No Hit
CCGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC	106	0.5191497698109511	No Hit
CCGACGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	70	0.34283475364874133	No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	66	0.32324419629738466	No Hit
CCGACGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT	48	0.23508668821627976	No Hit
CCGACGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC	46	0.22529140954060142	No Hit
CCGACGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGAGCTCGTATGCCGT	40	0.19590557351356644	Illumina Single End Adapter 2 (95% over 24bp)
CCGACGGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC	36	0.17631501616220982	No Hit
CCGACGGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	36	0.17631501616220982	No Hit
CCGACGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	31	0.15182681947301402	No Hit
CCGACGGGGTGCTCCGACGGGGTGCTCATTCTGGTTCACCCTGCCCCTGCCCCCGC	31	0.15182681947301402	No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	29	0.14203154079733568	No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC	27	0.13223626212165734	No Hit
CCGACGGGGACAGAAAAATGACTTTAGTTATGTAATATGCTTAATTCTCATTCTCT	24	0.11754334410813988	No Hit
CCGACGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGACGAGCTCGTATGCCGT	24	0.11754334410813988	Illumina Single End Adapter 1 (95% over 23bp)
CCGACGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGATATCGTATGCCGT	23	0.11264570477030071	No Hit
CCGACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCA	23	0.11264570477030071	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.004897639337839162	0.0	0.0	0.0	0.0
14	0.03428347536487413	0.0	0.0	0.0	0.0
15	0.03428347536487413	0.0	0.0	0.0	0.0
16	0.03428347536487413	0.0	0.0	0.0	0.0
17	0.03918111470271329	0.0	0.0	0.0	0.0
18	0.03918111470271329	0.0	0.0	0.0	0.0
19	0.044078754040552455	0.0	0.0	0.0	0.0
20	0.044078754040552455	0.0	0.0	0.0	0.0
21	0.044078754040552455	0.0	0.0	0.0	0.0
22	0.044078754040552455	0.0	0.0	0.0	0.0
23	0.04897639337839162	0.0	0.0	0.0	0.0
24	0.05387403271623078	0.0	0.0	0.0	0.0
25	0.05387403271623078	0.0	0.0	0.0	0.0
26	0.05387403271623078	0.0	0.0	0.0	0.0
27	0.06856695072974826	0.0	0.0	0.0	0.0
28	0.07836222940542659	0.0	0.0	0.0	0.0
29	0.08325986874326574	0.0	0.0	0.0	0.0
30	0.08815750808110491	0.0	0.0	0.0	0.0
31	0.10774806543246156	0.0	0.0	0.0	0.0
32	0.10774806543246156	0.0	0.0	0.0	0.0
33	0.10774806543246156	0.0	0.0	0.0	0.0
34	0.10774806543246156	0.0	0.0	0.0	0.0
35	0.11264570477030071	0.0	0.0	0.0	0.0
36	0.11264570477030071	0.0	0.0	0.0	0.0
37	0.11264570477030071	0.0	0.0	0.0	0.0
38	0.11754334410813988	0.0	0.0	0.0	0.0
39	0.12244098344597903	0.0	0.0	0.0	0.0
40	0.12244098344597903	0.0	0.0	0.0	0.0
41	0.12244098344597903	0.0	0.0	0.0	0.0
42	0.13713390145949653	0.0	0.0	0.0	0.0
43	0.15672445881085317	0.0	0.0	0.0	0.0
44	0.16651973748653148	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCATT	25	1.9203446E-5	50.406948	38
CGCATTG	25	1.9203446E-5	50.406948	39
GCATTGA	25	1.9203446E-5	50.406948	40
GCTCAAT	15	0.008049961	50.406948	39
TGTTCCC	20	3.9258267E-4	50.406944	38
TAATCCG	15	0.008129502	50.282177	42
CCCTCCG	20	3.9743714E-4	50.282177	42
AGAGCCC	15	0.008209626	50.158024	36
GGGCGCA	25	1.9777739E-5	50.158024	36
TTTGATC	20	4.023392E-4	50.158024	36
CAGTGGT	25	2.0070178E-5	50.034485	13
CTGCGGG	15	0.008290336	50.03448	49
CAAATCC	15	0.008290336	50.03448	13
CGGGGCA	65	0.0	49.78922	5
GGCACCC	15	0.008453526	49.789215	8
CGGGGTG	30	1.0271906E-6	49.789215	5
CGGGGGA	195	0.0	49.789215	5
CGGGGCT	60	0.0	49.789215	5
CGGGGCC	20	4.1733432E-4	49.789215	5
CGGGGAC	60	0.0	49.789215	5
>>END_MODULE