FastQCFastQC Report
Fri 17 Jun 2016
SRR1525865_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525865_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5634
Sequences flagged as poor quality0
Sequence length40
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC160.2839900603478878No Hit
CCGACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT150.26624068157614483No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC90.1597444089456869No Hit
CCGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG90.1597444089456869No Hit
CCGACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT90.1597444089456869No Hit
CCGACGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT80.1419950301739439No Hit
CCGACGGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG80.1419950301739439No Hit
CCGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC80.1419950301739439No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTAGC251.6500689E-434.43636320
GATTTAG251.7423899E-434.12612519
GGTTGGG251.838938E-433.8214312
CGGGGGG950.033.821435
CGGGGAC502.928573E-1033.821435
TTGGGGA251.838938E-433.8214314
TGGGGAT251.838938E-433.8214315
GGGGGAG251.838938E-433.821436
ACGGGGT502.928573E-1033.821434
GGAAGCA251.838938E-433.821438
GTTGGGG251.838938E-433.8214313
GGGAAGC251.838938E-433.821437
CGGGGTC200.002626744833.8214265
CGGGGGT358.930874E-733.8214265
CGGGGGC200.002626744833.8214265
CGGGGGA700.033.8214265
CGGGGCT200.002626744833.8214265
CGGGGAT406.186565E-833.8214265
CGGGGAA454.263711E-933.8214265
GACGGGA200.002626744833.8214263
Produced by FastQC (version 0.11.5)