FastQCFastQC Report
Fri 17 Jun 2016
SRR1525863_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525863_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1053
Sequences flagged as poor quality0
Sequence length40
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT535.0332383665717No Hit
CCGACGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG272.564102564102564No Hit
CCGACGGGGTCCCAGATCCTCTTCTGAAGGAGATTGGGCG60.5698005698005698No Hit
CCGACGGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT60.5698005698005698No Hit
CCGACGGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT60.5698005698005698No Hit
CCGACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.5698005698005698No Hit
CCGACGGGGTCACAGATACTCTTCTGAAGGAGATTGGGCG50.4748338081671415No Hit
CCGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT50.4748338081671415No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC50.4748338081671415No Hit
CCGACGGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT40.3798670465337132No Hit
CCGACGGGGGTTCATTTCCATGATGTTTATGCCACTATTA40.3798670465337132No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT40.3798670465337132No Hit
CCGACGGGGGTTTATGCCACTATTAAACCAGTGGTCATGT40.3798670465337132No Hit
CCGACGGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCG30.2849002849002849No Hit
CCGACGGGGATGGGATTCAGGTTGAGCCCCCAAACTGTGA30.2849002849002849No Hit
CCGACGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT30.2849002849002849No Hit
CCGACGGGGGTCCTCACACTAGCACTGATGGGGGCTGCGG20.1899335232668566No Hit
CCGACGGGGTCAAAGATACTCTTCTGAGATGAGTTTTTGT20.1899335232668566No Hit
CCGACGGGGGGTTCATCAGTGGCAACAACTCCTAGAGTGT20.1899335232668566No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG20.1899335232668566No Hit
CCGACGGGGAAAGATGTGGGTGTATTTTACAGCTACACAA20.1899335232668566No Hit
CCGACGGGGGTATCAACGCAGAGTTGACTTTTTTTTTTTT20.1899335232668566No Hit
CCGACGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA20.1899335232668566No Hit
CCGACGGGGTCACAGATCCTCTTATGAGATGAGTTTTTGT20.1899335232668566No Hit
CCGACGGGGTCACAGACCCTCTTCTGAGATGAGTTTTTGT20.1899335232668566No Hit
CCGACGGGGATTCTATTTCTTTCAAAGGCTACCAGCCATT20.1899335232668566No Hit
CCGACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1899335232668566No Hit
CCGACGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGA20.1899335232668566No Hit
CCGACGGGGTCTTGGTGTGTGGGGGATCTATGGCCATCTC20.1899335232668566No Hit
CCGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA20.1899335232668566No Hit
CCGACGGGGCAAATGAGAAATGTTCTTTTATATTGTGTTG20.1899335232668566No Hit
CCGACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT20.1899335232668566No Hit
CCGACGGGGACCAAACATGCTGCTGTTGTTGTTATTATCG20.1899335232668566No Hit
CCGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG20.1899335232668566No Hit
CCGACGGGGGAGATGGCCCGGATCCTCCGGCCAGGGGGCT20.1899335232668566No Hit
CCGACGGGGTCCCAGATCCTCTTCTGAGATGAGTTTTTGT20.1899335232668566No Hit
CCGACGGGGATCTTAGTGTATATTTATTTATATGACAGAT20.1899335232668566No Hit
CCGACGGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG20.1899335232668566No Hit
CCGACGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1899335232668566No Hit
CCGACGGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTG20.1899335232668566No Hit
CCGACGGGGCTCCGACGGGGTCACAGCTCCTCTTCTGAGA20.1899335232668566No Hit
CCGACGGGGAAGCAGTGGCATCAACGCAAAAAAAAAAAAA20.1899335232668566No Hit
CCGACGGGGACTAATCCTAGCCCTAGCCCTACACAAATAT20.1899335232668566No Hit
CCGACGGGGGGCCTGGGCCTTTCAGGAATACCACGACGCT20.1899335232668566No Hit
CCGACGGGGGATCATCTTCTGAGATGAGTTTTTGTTCAAT20.1899335232668566No Hit
CCGACGGGGATTGATGCTCAGTTTCAAAGTGAGCCACCAG20.1899335232668566No Hit
CCGACGGGGGCTGAGACCAGTGTTTCTGTTGTGATCCTGA20.1899335232668566No Hit
CCGACGGGGGTTGGTGCCTGTACTTGAACACCTTACAGGA20.1899335232668566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTCT305.0846265E-634.016
TCCTCTT305.0846265E-634.017
GGGTCAC259.5802614E-534.07
CTGAGAT305.0846265E-634.024
GTCACAG305.0846265E-634.09
ACAGATC259.5802614E-534.012
CGGGGTC352.539673E-734.05
GACGGGG850.034.03
CTCTTCT401.1868906E-834.019
TTTTTGT200.001707216534.034
GTTTTTG200.001707216534.033
TTCTGAG305.0846265E-634.022
GGTCACA305.0846265E-634.08
GAGTTTT259.5802614E-534.031
GATGAGT305.0846265E-634.028
CCTCTTC305.0846265E-634.018
GATCCTC305.0846265E-634.015
TCTGAGA305.0846265E-634.023
ACGGGGT352.539673E-734.04
ACGGGGG401.1868906E-834.04
Produced by FastQC (version 0.11.5)