##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525863_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1053 Sequences flagged as poor quality 0 Sequence length 40 %GC 53 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.23931623931624 30.0 4.0 33.0 4.0 35.0 2 18.03133903133903 20.0 4.0 30.0 4.0 33.0 3 15.368471035137702 14.0 4.0 26.0 4.0 33.0 4 12.061728395061728 8.0 4.0 19.0 4.0 29.0 5 15.870845204178538 15.0 4.0 28.0 4.0 33.0 6 13.654320987654321 4.0 4.0 25.0 4.0 32.0 7 14.414055080721747 4.0 4.0 27.0 4.0 32.0 8 13.522317188983855 4.0 4.0 25.0 4.0 31.0 9 13.70275403608737 4.0 4.0 25.0 4.0 33.0 10 13.132003798670466 4.0 4.0 25.0 4.0 32.0 11 11.592592592592593 4.0 4.0 22.0 4.0 29.0 12 11.305792972459638 4.0 4.0 20.0 4.0 30.0 13 10.901234567901234 4.0 4.0 18.0 4.0 29.0 14 10.768281101614434 4.0 4.0 18.0 4.0 30.0 15 10.996201329534664 4.0 4.0 19.0 4.0 31.0 16 9.481481481481481 4.0 4.0 12.0 4.0 28.0 17 9.402659069325736 4.0 4.0 14.0 4.0 26.0 18 9.125356125356126 4.0 4.0 12.0 4.0 25.0 19 9.04653371320038 4.0 4.0 11.0 4.0 25.0 20 9.037037037037036 4.0 4.0 10.0 4.0 27.0 21 8.814814814814815 4.0 4.0 8.0 4.0 27.0 22 8.678062678062679 4.0 4.0 9.0 4.0 26.0 23 9.169990503323836 4.0 4.0 8.0 4.0 28.0 24 8.57454890788224 4.0 4.0 4.0 4.0 26.0 25 9.122507122507123 4.0 4.0 4.0 4.0 28.0 26 8.752136752136753 4.0 4.0 4.0 4.0 26.0 27 8.651471984805319 4.0 4.0 4.0 4.0 27.0 28 8.94491927825261 4.0 4.0 4.0 4.0 28.0 29 8.095916429249762 4.0 4.0 4.0 4.0 23.0 30 8.87369420702754 4.0 4.0 4.0 4.0 27.0 31 9.149097815764483 4.0 4.0 4.0 4.0 30.0 32 9.326685660018994 4.0 4.0 4.0 4.0 30.0 33 9.2659069325736 4.0 4.0 4.0 4.0 30.0 34 9.295346628679962 4.0 4.0 4.0 4.0 31.0 35 8.963912630579298 4.0 4.0 4.0 4.0 30.0 36 8.721747388414055 4.0 4.0 4.0 4.0 30.0 37 8.675213675213675 4.0 4.0 4.0 4.0 30.0 38 8.385565052231719 4.0 4.0 4.0 4.0 29.0 39 8.386514719848053 4.0 4.0 4.0 4.0 28.0 40 8.540360873694206 4.0 4.0 4.0 4.0 31.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 14.281862745098039 2 2 17.231617647058822 2 3 7.792892156862743 2 4 7.012254901960786 2 5 17.44362745098039 2 6 2.561274509803921 2 7 6.232843137254903 2 8 5.264705882352942 2 9 -7.350490196078431 2 10 6.36029411764706 2 11 3.5857843137254903 2 12 8.637254901960786 2 13 9.71078431372549 2 14 12.754901960784313 2 15 18.470588235294116 2 16 18.090686274509807 2 17 19.997549019607845 2 18 0.4264705882352935 2 19 21.833333333333332 2 20 23.424019607843135 2 21 -4.8014705882352935 2 22 -4.742647058823529 2 23 -4.943627450980392 2 24 -4.727941176470589 2 25 -5.377450980392158 2 26 -3.272058823529412 2 27 -3.090686274509804 2 28 -3.4607843137254903 2 29 -2.306372549019608 2 30 -4.029411764705882 2 31 -3.813725490196079 2 32 -3.9485294117647056 2 33 -4.044117647058824 2 34 -3.799019607843136 2 35 -3.6911764705882355 2 36 -4.137254901960784 2 37 -3.5882352941176467 2 38 -4.186274509803923 2 39 -3.8872549019607847 2 40 -4.286764705882353 3 1 -17.71813725490196 3 2 -13.768382352941178 3 3 -11.207107843137257 3 4 -7.987745098039214 3 5 -11.55637254901961 3 6 -9.438725490196079 3 7 -9.767156862745097 3 8 -9.735294117647058 3 9 -9.35049019607843 3 10 -9.63970588235294 3 11 -8.41421568627451 3 12 -8.362745098039214 3 13 -7.28921568627451 3 14 -7.245098039215687 3 15 -7.529411764705882 3 16 -5.909313725490195 3 17 -6.002450980392156 3 18 -5.5735294117647065 3 19 -5.166666666666668 3 20 -5.575980392156863 3 21 -4.8014705882352935 3 22 -4.742647058823529 3 23 -4.943627450980392 3 24 -4.727941176470589 3 25 -5.377450980392158 3 26 -3.272058823529412 3 27 -3.090686274509804 3 28 -3.4607843137254903 3 29 -2.306372549019608 3 30 -4.029411764705882 3 31 -3.813725490196079 3 32 -3.9485294117647056 3 33 -4.044117647058824 3 34 -3.799019607843136 3 35 -3.6911764705882355 3 36 -4.137254901960784 3 37 -3.5882352941176467 3 38 -4.186274509803923 3 39 -3.8872549019607847 3 40 -4.286764705882353 4 1 4.281862745098039 4 2 7.2316176470588225 4 3 6.792892156862743 4 4 -1.9877450980392144 4 5 1.4436274509803901 4 6 3.561274509803921 4 7 10.232843137254903 4 8 10.264705882352942 4 9 13.64950980392157 4 10 1.3602941176470598 4 11 11.58578431372549 4 12 6.637254901960786 4 13 3.7107843137254903 4 14 -2.245098039215687 4 15 17.470588235294116 4 16 2.090686274509805 4 17 6.997549019607844 4 18 16.426470588235293 4 19 6.833333333333332 4 20 16.424019607843135 4 21 10.198529411764707 4 22 14.257352941176471 4 23 20.056372549019606 4 24 3.2720588235294112 4 25 8.622549019607842 4 26 2.727941176470588 4 27 8.909313725490197 4 28 18.53921568627451 4 29 1.693627450980392 4 30 11.970588235294118 4 31 15.186274509803921 4 32 9.051470588235293 4 33 16.955882352941174 4 34 5.200980392156864 4 35 22.308823529411764 4 36 29.862745098039216 4 37 30.411764705882355 4 38 29.813725490196077 4 39 30.112745098039216 4 40 29.713235294117645 5 1 -17.71813725490196 5 2 -13.768382352941178 5 3 -11.207107843137257 5 4 -7.987745098039214 5 5 -11.55637254901961 5 6 -9.438725490196079 5 7 -9.767156862745097 5 8 -9.735294117647058 5 9 -9.35049019607843 5 10 -9.63970588235294 5 11 -8.41421568627451 5 12 -8.362745098039214 5 13 -7.28921568627451 5 14 -7.245098039215687 5 15 -7.529411764705882 5 16 -5.909313725490195 5 17 -6.002450980392156 5 18 -5.5735294117647065 5 19 -5.166666666666668 5 20 -5.575980392156863 5 21 -4.8014705882352935 5 22 -4.742647058823529 5 23 -4.943627450980392 5 24 -4.727941176470589 5 25 -5.377450980392158 5 26 -3.272058823529412 5 27 -3.090686274509804 5 28 -3.4607843137254903 5 29 -2.306372549019608 5 30 -4.029411764705882 5 31 -3.813725490196079 5 32 -3.9485294117647056 5 33 -4.044117647058824 5 34 -3.799019607843136 5 35 -3.6911764705882355 5 36 -4.137254901960784 5 37 -3.5882352941176467 5 38 -4.186274509803923 5 39 -3.8872549019607847 5 40 -4.286764705882353 7 1 7.281862745098039 7 2 0.7316176470588225 7 3 6.792892156862743 7 4 -1.4877450980392144 7 5 4.44362745098039 7 6 0.5612745098039209 7 7 4.232843137254903 7 8 9.764705882352942 7 9 3.6495098039215694 7 10 11.86029411764706 7 11 7.08578431372549 7 12 7.637254901960786 7 13 0.7107843137254903 7 14 3.254901960784313 7 15 -5.529411764705882 7 16 -1.409313725490195 7 17 -0.00245098039215641 7 18 3.9264705882352935 7 19 6.833333333333332 7 20 -2.575980392156863 7 21 10.198529411764707 7 22 6.257352941176471 7 23 1.556372549019608 7 24 9.272058823529411 7 25 7.122549019607842 7 26 7.227941176470588 7 27 1.409313725490196 7 28 8.53921568627451 7 29 0.6936274509803919 7 30 9.470588235294118 7 31 5.686274509803921 7 32 8.051470588235293 7 33 4.955882352941176 7 34 9.700980392156865 7 35 8.308823529411764 7 36 12.862745098039216 7 37 13.411764705882353 7 38 12.813725490196077 7 39 13.112745098039216 7 40 11.213235294117647 8 1 -17.71813725490196 8 2 -13.768382352941178 8 3 -11.207107843137257 8 4 -7.987745098039214 8 5 -11.55637254901961 8 6 -9.438725490196079 8 7 -9.767156862745097 8 8 -9.735294117647058 8 9 -9.35049019607843 8 10 -9.63970588235294 8 11 -8.41421568627451 8 12 -8.362745098039214 8 13 -7.28921568627451 8 14 -7.245098039215687 8 15 -7.529411764705882 8 16 -5.909313725490195 8 17 -6.002450980392156 8 18 -5.5735294117647065 8 19 -5.166666666666668 8 20 -5.575980392156863 8 21 -4.8014705882352935 8 22 -4.742647058823529 8 23 -4.943627450980392 8 24 -4.727941176470589 8 25 -5.377450980392158 8 26 -3.272058823529412 8 27 -3.090686274509804 8 28 -3.4607843137254903 8 29 -2.306372549019608 8 30 -4.029411764705882 8 31 -3.813725490196079 8 32 -3.9485294117647056 8 33 -4.044117647058824 8 34 -3.799019607843136 8 35 -3.6911764705882355 8 36 -4.137254901960784 8 37 -3.5882352941176467 8 38 -4.186274509803923 8 39 -3.8872549019607847 8 40 -4.286764705882353 9 1 16.28186274509804 9 2 19.231617647058822 9 3 19.79289215686274 9 4 22.012254901960787 9 5 21.94362745098039 9 6 22.561274509803923 9 7 22.232843137254903 9 8 16.764705882352942 9 9 23.649509803921568 9 10 10.36029411764706 9 11 11.08578431372549 9 12 16.137254901960787 9 13 3.7107843137254903 9 14 19.754901960784313 9 15 8.970588235294118 9 16 6.090686274509805 9 17 -1.5024509803921564 9 18 10.926470588235293 9 19 8.333333333333332 9 20 4.424019607843137 9 21 11.698529411764707 9 22 7.257352941176471 9 23 14.556372549019608 9 24 16.772058823529413 9 25 19.122549019607842 9 26 8.727941176470587 9 27 22.409313725490197 9 28 9.53921568627451 9 29 13.693627450980392 9 30 12.470588235294118 9 31 13.186274509803921 9 32 23.551470588235293 9 33 20.955882352941174 9 34 13.700980392156865 9 35 15.808823529411764 9 36 15.862745098039216 9 37 13.411764705882353 9 38 16.313725490196077 9 39 14.612745098039216 9 40 22.213235294117645 10 1 -17.71813725490196 10 2 -13.768382352941178 10 3 -11.207107843137257 10 4 -7.987745098039214 10 5 -11.55637254901961 10 6 -9.438725490196079 10 7 -9.767156862745097 10 8 -9.735294117647058 10 9 -9.35049019607843 10 10 -9.63970588235294 10 11 -8.41421568627451 10 12 -8.362745098039214 10 13 -7.28921568627451 10 14 -7.245098039215687 10 15 -7.529411764705882 10 16 -5.909313725490195 10 17 -6.002450980392156 10 18 -5.5735294117647065 10 19 -5.166666666666668 10 20 -5.575980392156863 10 21 -4.8014705882352935 10 22 -4.742647058823529 10 23 -4.943627450980392 10 24 -4.727941176470589 10 25 -5.377450980392158 10 26 -3.272058823529412 10 27 -3.090686274509804 10 28 -3.4607843137254903 10 29 -2.306372549019608 10 30 -4.029411764705882 10 31 -3.813725490196079 10 32 -3.9485294117647056 10 33 -4.044117647058824 10 34 -3.799019607843136 10 35 -3.6911764705882355 10 36 -4.137254901960784 10 37 -3.5882352941176467 10 38 -4.186274509803923 10 39 -3.8872549019607847 10 40 -4.286764705882353 12 1 8.281862745098039 12 2 12.231617647058822 12 3 5.792892156862743 12 4 14.012254901960786 12 5 2.44362745098039 12 6 6.561274509803921 12 7 17.232843137254903 12 8 11.264705882352942 12 9 11.64950980392157 12 10 15.36029411764706 12 11 18.58578431372549 12 12 14.637254901960786 12 13 6.71078431372549 12 14 8.754901960784313 12 15 19.470588235294116 12 16 -5.909313725490195 12 17 -6.002450980392156 12 18 -5.5735294117647065 12 19 -5.166666666666668 12 20 -5.575980392156863 12 21 -4.8014705882352935 12 22 -4.742647058823529 12 23 -4.943627450980392 12 24 -4.727941176470589 12 25 -5.377450980392158 12 26 -3.272058823529412 12 27 -3.090686274509804 12 28 -3.4607843137254903 12 29 -2.306372549019608 12 30 -4.029411764705882 12 31 -3.813725490196079 12 32 -3.9485294117647056 12 33 -4.044117647058824 12 34 -3.799019607843136 12 35 -3.6911764705882355 12 36 -4.137254901960784 12 37 -3.5882352941176467 12 38 -4.186274509803923 12 39 -3.8872549019607847 12 40 -4.286764705882353 14 1 5.281862745098039 14 2 -6.7683823529411775 14 3 -0.2071078431372566 14 4 -0.9877450980392144 14 5 11.44362745098039 14 6 -2.438725490196079 14 7 -3.7671568627450966 14 8 8.264705882352942 14 9 -4.350490196078431 14 10 1.3602941176470598 14 11 12.58578431372549 14 12 12.637254901960786 14 13 9.71078431372549 14 14 9.754901960784313 14 15 20.470588235294116 14 16 19.090686274509807 14 17 21.997549019607845 14 18 16.426470588235293 14 19 20.833333333333332 14 20 2.424019607843137 14 21 8.198529411764707 14 22 16.25735294117647 14 23 -0.9436274509803919 14 24 18.272058823529413 14 25 16.622549019607842 14 26 20.727941176470587 14 27 4.909313725490196 14 28 24.53921568627451 14 29 6.693627450980392 14 30 23.970588235294116 14 31 28.186274509803923 14 32 27.051470588235293 14 33 26.955882352941174 14 34 18.200980392156865 14 35 7.3088235294117645 14 36 8.862745098039216 14 37 2.4117647058823533 14 38 21.813725490196077 14 39 9.112745098039216 14 40 24.713235294117645 15 1 -17.71813725490196 15 2 -13.768382352941178 15 3 -11.207107843137257 15 4 -7.987745098039214 15 5 -11.55637254901961 15 6 -9.438725490196079 15 7 -9.767156862745097 15 8 -9.735294117647058 15 9 -9.35049019607843 15 10 -9.63970588235294 15 11 -8.41421568627451 15 12 -8.362745098039214 15 13 -7.28921568627451 15 14 -7.245098039215687 15 15 -7.529411764705882 15 16 -5.909313725490195 15 17 -6.002450980392156 15 18 -5.5735294117647065 15 19 -5.166666666666668 15 20 -5.575980392156863 15 21 -4.8014705882352935 15 22 -4.742647058823529 15 23 -4.943627450980392 15 24 -4.727941176470589 15 25 -5.377450980392158 15 26 -3.272058823529412 15 27 -3.090686274509804 15 28 -3.4607843137254903 15 29 -2.306372549019608 15 30 -4.029411764705882 15 31 -3.813725490196079 15 32 -3.9485294117647056 15 33 -4.044117647058824 15 34 -3.799019607843136 15 35 -3.6911764705882355 15 36 -4.137254901960784 15 37 -3.5882352941176467 15 38 -4.186274509803923 15 39 -3.8872549019607847 15 40 -4.286764705882353 16 1 10.948529411764703 16 2 -1.4350490196078454 16 3 12.792892156862743 16 4 18.012254901960787 16 5 12.44362745098039 16 6 8.894607843137253 16 7 15.232843137254903 16 8 21.264705882352942 16 9 20.316176470588232 16 10 18.360294117647058 16 11 20.252450980392155 16 12 18.30392156862745 16 13 15.71078431372549 16 14 23.08823529411765 16 15 20.80392156862745 16 16 12.424019607843137 16 17 11.997549019607844 16 18 16.09313725490196 16 19 9.833333333333332 16 20 13.424019607843137 16 21 20.53186274509804 16 22 13.924019607843139 16 23 14.38970588235294 16 24 9.272058823529411 16 25 10.955882352941174 16 26 17.727941176470587 16 27 21.575980392156865 16 28 22.53921568627451 16 29 13.693627450980392 16 30 12.970588235294118 16 31 18.186274509803923 16 32 23.384803921568626 16 33 16.955882352941174 16 34 17.867647058823533 16 35 22.642156862745097 16 36 16.529411764705884 16 37 25.411764705882355 16 38 21.14705882352941 16 39 15.779411764705884 16 40 14.713235294117647 17 1 8.281862745098039 17 2 -5.7683823529411775 17 3 14.792892156862743 17 4 -0.9877450980392144 17 5 5.44362745098039 17 6 9.561274509803921 17 7 10.232843137254903 17 8 5.264705882352942 17 9 3.6495098039215694 17 10 15.36029411764706 17 11 25.58578431372549 17 12 25.637254901960787 17 13 24.71078431372549 17 14 26.754901960784313 17 15 26.470588235294116 17 16 20.090686274509807 17 17 20.997549019607845 17 18 26.426470588235293 17 19 22.833333333333332 17 20 22.424019607843135 17 21 19.198529411764707 17 22 8.257352941176471 17 23 9.056372549019608 17 24 19.272058823529413 17 25 23.622549019607842 17 26 16.727941176470587 17 27 21.909313725490197 17 28 7.53921568627451 17 29 6.693627450980392 17 30 13.970588235294118 17 31 17.186274509803923 17 32 17.051470588235293 17 33 22.955882352941174 17 34 19.200980392156865 17 35 24.308823529411764 17 36 14.862745098039216 17 37 22.411764705882355 17 38 21.813725490196077 17 39 11.112745098039216 17 40 24.713235294117645 18 1 -10.384803921568627 18 2 -6.7683823529411775 18 3 -8.54044117647059 18 4 -5.654411764705881 18 5 -4.223039215686276 18 6 -6.105392156862746 18 7 -6.433823529411764 18 8 -6.735294117647058 18 9 -6.350490196078431 18 10 -6.973039215686273 18 11 0.5857843137254903 18 12 0.6372549019607856 18 13 1.7107843137254903 18 14 1.7549019607843128 18 15 1.4705882352941178 18 16 0.42401960784313886 18 17 3.9975490196078436 18 18 4.759803921568627 18 19 -5.166666666666668 18 20 -5.575980392156863 18 21 -4.8014705882352935 18 22 -4.742647058823529 18 23 -4.943627450980392 18 24 -4.727941176470589 18 25 -5.377450980392158 18 26 -3.272058823529412 18 27 -3.090686274509804 18 28 -3.4607843137254903 18 29 -2.306372549019608 18 30 -4.029411764705882 18 31 -3.813725490196079 18 32 -3.9485294117647056 18 33 -4.044117647058824 18 34 -3.799019607843136 18 35 -3.6911764705882355 18 36 -4.137254901960784 18 37 -3.5882352941176467 18 38 -4.186274509803923 18 39 -3.8872549019607847 18 40 -4.286764705882353 19 1 0.9485294117647065 19 2 -3.1017156862745114 19 3 2.1262254901960755 19 4 1.0122549019607856 19 5 2.7769607843137223 19 6 8.227941176470589 19 7 9.232843137254903 19 8 10.598039215686274 19 9 11.316176470588237 19 10 9.36029411764706 19 11 7.252450980392158 19 12 9.303921568627453 19 13 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-3.8872549019607847 116 40 -4.286764705882353 117 1 8.281862745098039 117 2 12.231617647058822 117 3 -11.207107843137257 117 4 -7.987745098039214 117 5 -11.55637254901961 117 6 -9.438725490196079 117 7 -9.767156862745097 117 8 -9.735294117647058 117 9 -9.35049019607843 117 10 -9.63970588235294 117 11 -8.41421568627451 117 12 -8.362745098039214 117 13 -7.28921568627451 117 14 -7.245098039215687 117 15 -7.529411764705882 117 16 -5.909313725490195 117 17 -6.002450980392156 117 18 -5.5735294117647065 117 19 -5.166666666666668 117 20 -5.575980392156863 117 21 -4.8014705882352935 117 22 -4.742647058823529 117 23 -4.943627450980392 117 24 -4.727941176470589 117 25 -5.377450980392158 117 26 -3.272058823529412 117 27 -3.090686274509804 117 28 -3.4607843137254903 117 29 -2.306372549019608 117 30 -4.029411764705882 117 31 -3.813725490196079 117 32 -3.9485294117647056 117 33 -4.044117647058824 117 34 -3.799019607843136 117 35 -3.6911764705882355 117 36 -4.137254901960784 117 37 -3.5882352941176467 117 38 -4.186274509803923 117 39 -3.8872549019607847 117 40 -4.286764705882353 119 1 -17.71813725490196 119 2 -13.768382352941178 119 3 -11.207107843137257 119 4 -7.987745098039214 119 5 -11.55637254901961 119 6 -9.438725490196079 119 7 -9.767156862745097 119 8 -9.735294117647058 119 9 -9.35049019607843 119 10 -9.63970588235294 119 11 -8.41421568627451 119 12 -8.362745098039214 119 13 -7.28921568627451 119 14 -7.245098039215687 119 15 -7.529411764705882 119 16 -5.909313725490195 119 17 -6.002450980392156 119 18 -5.5735294117647065 119 19 -5.166666666666668 119 20 -5.575980392156863 119 21 -4.8014705882352935 119 22 -4.742647058823529 119 23 -4.943627450980392 119 24 -4.727941176470589 119 25 -5.377450980392158 119 26 -3.272058823529412 119 27 -3.090686274509804 119 28 -3.4607843137254903 119 29 -2.306372549019608 119 30 -4.029411764705882 119 31 -3.813725490196079 119 32 -3.9485294117647056 119 33 -4.044117647058824 119 34 -3.799019607843136 119 35 -3.6911764705882355 119 36 -4.137254901960784 119 37 -3.5882352941176467 119 38 -4.186274509803923 119 39 -3.8872549019607847 119 40 -4.286764705882353 120 1 -17.71813725490196 120 2 -13.768382352941178 120 3 -11.207107843137257 120 4 -7.987745098039214 120 5 -11.55637254901961 120 6 -9.438725490196079 120 7 -9.767156862745097 120 8 -9.735294117647058 120 9 -9.35049019607843 120 10 -9.63970588235294 120 11 -8.41421568627451 120 12 -8.362745098039214 120 13 -7.28921568627451 120 14 -7.245098039215687 120 15 -7.529411764705882 120 16 -5.909313725490195 120 17 -6.002450980392156 120 18 -5.5735294117647065 120 19 -5.166666666666668 120 20 -5.575980392156863 120 21 -4.8014705882352935 120 22 -4.742647058823529 120 23 -4.943627450980392 120 24 -4.727941176470589 120 25 -5.377450980392158 120 26 -3.272058823529412 120 27 -3.090686274509804 120 28 -3.4607843137254903 120 29 -2.306372549019608 120 30 -4.029411764705882 120 31 -3.813725490196079 120 32 -3.9485294117647056 120 33 -4.044117647058824 120 34 -3.799019607843136 120 35 -3.6911764705882355 120 36 -4.137254901960784 120 37 -3.5882352941176467 120 38 -4.186274509803923 120 39 -3.8872549019607847 120 40 -4.286764705882353 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 414.0 5 62.0 6 46.0 7 40.0 8 33.0 9 43.0 10 42.0 11 35.0 12 20.0 13 18.0 14 23.0 15 19.0 16 11.0 17 6.0 18 6.0 19 13.0 20 15.0 21 30.0 22 40.0 23 31.0 24 27.0 25 29.0 26 17.0 27 16.0 28 8.0 29 3.0 30 3.0 31 1.0 32 1.0 33 0.0 34 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 99.05033238366572 0.7597340930674265 0.0949667616334283 0.0949667616334283 8 97.8116079923882 1.0466222645099905 0.6660323501427212 0.47573739295908657 9 94.00570884871551 1.8078020932445291 3.2350142721217887 0.9514747859181731 10 38.84140550807217 23.266856600189932 26.875593542260205 11.016144349477683 11 25.166191832858498 23.076923076923077 19.943019943019944 31.813865147198477 12 27.54036087369421 32.95346628679962 20.322886989553655 19.183285849952515 13 18.043684710351375 23.36182336182336 22.41215574548908 36.182336182336186 14 17.094017094017094 45.77397910731244 23.076923076923077 14.055080721747387 15 39.12630579297246 26.495726495726498 23.646723646723647 10.731244064577398 16 23.741690408357073 41.02564102564102 24.216524216524217 11.016144349477683 17 22.31718898385565 22.41215574548908 39.69610636277303 15.57454890788224 18 20.417853751187085 23.36182336182336 24.406457739791072 31.813865147198477 19 18.708452041785375 22.507122507122507 24.691358024691358 34.09306742640076 20 19.943019943019944 26.305792972459642 38.65147198480532 15.0997150997151 21 16.714150047483383 21.557454890788225 28.49002849002849 33.238366571699906 22 16.334283000949668 21.367521367521366 45.77397910731244 16.524216524216524 23 17.28395061728395 23.741690408357073 40.83570750237417 18.138651471984804 24 15.004748338081672 28.8698955365622 24.976258309591643 31.14909781576448 25 20.437262357414447 24.809885931558938 36.40684410646388 18.346007604562736 26 39.25501432664756 26.456542502387776 19.961795606494746 14.326647564469914 27 19.029495718363464 41.674595623215986 19.790675547098 19.50523311132255 28 28.517110266159694 34.31558935361217 19.866920152091254 17.300380228136884 29 27.645376549094376 33.74642516682555 22.4976167778837 16.11058150619638 30 24.596391263057928 27.350427350427353 33.71320037986705 14.339981006647673 31 29.182509505703425 31.1787072243346 24.23954372623574 15.399239543726237 32 27.411652340019106 34.47946513849093 24.164278892072588 13.944603629417381 33 33.33333333333333 31.14909781576448 21.27255460588794 14.245014245014245 34 19.943019943019944 24.406457739791072 41.88034188034188 13.770180436847104 35 16.80911680911681 25.8309591642925 42.64007597340931 14.719848053181387 36 22.88698955365622 25.546058879392213 35.51756885090219 16.049382716049383 37 27.376425855513308 26.140684410646386 31.55893536121673 14.923954372623575 38 26.685660018993353 24.406457739791072 33.80816714150047 15.0997150997151 39 29.34472934472934 24.691358024691358 24.691358024691358 21.27255460588794 40 23.36182336182336 24.121557454890787 37.70180436847104 14.814814814814813 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 3.0 20 6.0 21 7.0 22 8.0 23 8.0 24 9.0 25 10.0 26 9.0 27 8.0 28 8.0 29 10.0 30 12.0 31 10.5 32 9.0 33 9.0 34 10.0 35 11.0 36 13.5 37 16.0 38 16.0 39 23.5 40 31.0 41 38.0 42 45.0 43 45.0 44 50.5 45 56.0 46 74.5 47 93.0 48 93.0 49 116.5 50 140.0 51 119.0 52 98.0 53 98.0 54 92.5 55 87.0 56 89.5 57 92.0 58 92.0 59 93.5 60 95.0 61 90.0 62 85.0 63 85.0 64 71.5 65 58.0 66 51.5 67 45.0 68 45.0 69 34.5 70 24.0 71 19.0 72 14.0 73 14.0 74 10.0 75 6.0 76 5.0 77 4.0 78 4.0 79 2.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.1899335232668566 9 0.1899335232668566 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0949667616334283 26 0.5698005698005698 27 0.1899335232668566 28 0.0949667616334283 29 0.3798670465337132 30 0.0 31 0.0949667616334283 32 0.5698005698005698 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0949667616334283 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 1053.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.80531813865147 #Duplication Level Percentage of deduplicated Percentage of total 1 94.62486002239642 80.24691358024691 2 3.5834266517357225 6.077872744539412 3 0.33594624860022393 0.8547008547008548 4 0.4479283314669653 1.519468186134853 5 0.33594624860022393 1.4245014245014245 6 0.4479283314669653 2.2792022792022792 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.11198208286674133 2.564102564102564 >50 0.11198208286674133 5.0332383665717 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCGACGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 53 5.0332383665717 No Hit CCGACGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG 27 2.564102564102564 No Hit CCGACGGGGTCCCAGATCCTCTTCTGAAGGAGATTGGGCG 6 0.5698005698005698 No Hit CCGACGGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT 6 0.5698005698005698 No Hit CCGACGGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT 6 0.5698005698005698 No Hit CCGACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.5698005698005698 No Hit CCGACGGGGTCACAGATACTCTTCTGAAGGAGATTGGGCG 5 0.4748338081671415 No Hit CCGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT 5 0.4748338081671415 No Hit CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 5 0.4748338081671415 No Hit CCGACGGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT 4 0.3798670465337132 No Hit CCGACGGGGGTTCATTTCCATGATGTTTATGCCACTATTA 4 0.3798670465337132 No Hit CCGACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT 4 0.3798670465337132 No Hit CCGACGGGGGTTTATGCCACTATTAAACCAGTGGTCATGT 4 0.3798670465337132 No Hit CCGACGGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCG 3 0.2849002849002849 No Hit CCGACGGGGATGGGATTCAGGTTGAGCCCCCAAACTGTGA 3 0.2849002849002849 No Hit CCGACGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT 3 0.2849002849002849 No Hit CCGACGGGGGTCCTCACACTAGCACTGATGGGGGCTGCGG 2 0.1899335232668566 No Hit CCGACGGGGTCAAAGATACTCTTCTGAGATGAGTTTTTGT 2 0.1899335232668566 No Hit CCGACGGGGGGTTCATCAGTGGCAACAACTCCTAGAGTGT 2 0.1899335232668566 No Hit CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG 2 0.1899335232668566 No Hit CCGACGGGGAAAGATGTGGGTGTATTTTACAGCTACACAA 2 0.1899335232668566 No Hit CCGACGGGGGTATCAACGCAGAGTTGACTTTTTTTTTTTT 2 0.1899335232668566 No Hit CCGACGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 2 0.1899335232668566 No Hit CCGACGGGGTCACAGATCCTCTTATGAGATGAGTTTTTGT 2 0.1899335232668566 No Hit CCGACGGGGTCACAGACCCTCTTCTGAGATGAGTTTTTGT 2 0.1899335232668566 No Hit CCGACGGGGATTCTATTTCTTTCAAAGGCTACCAGCCATT 2 0.1899335232668566 No Hit CCGACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.1899335232668566 No Hit CCGACGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGA 2 0.1899335232668566 No Hit CCGACGGGGTCTTGGTGTGTGGGGGATCTATGGCCATCTC 2 0.1899335232668566 No Hit CCGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA 2 0.1899335232668566 No Hit CCGACGGGGCAAATGAGAAATGTTCTTTTATATTGTGTTG 2 0.1899335232668566 No Hit CCGACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 2 0.1899335232668566 No Hit CCGACGGGGACCAAACATGCTGCTGTTGTTGTTATTATCG 2 0.1899335232668566 No Hit CCGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG 2 0.1899335232668566 No Hit CCGACGGGGGAGATGGCCCGGATCCTCCGGCCAGGGGGCT 2 0.1899335232668566 No Hit CCGACGGGGTCCCAGATCCTCTTCTGAGATGAGTTTTTGT 2 0.1899335232668566 No Hit CCGACGGGGATCTTAGTGTATATTTATTTATATGACAGAT 2 0.1899335232668566 No Hit CCGACGGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG 2 0.1899335232668566 No Hit CCGACGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.1899335232668566 No Hit CCGACGGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTG 2 0.1899335232668566 No Hit CCGACGGGGCTCCGACGGGGTCACAGCTCCTCTTCTGAGA 2 0.1899335232668566 No Hit CCGACGGGGAAGCAGTGGCATCAACGCAAAAAAAAAAAAA 2 0.1899335232668566 No Hit CCGACGGGGACTAATCCTAGCCCTAGCCCTACACAAATAT 2 0.1899335232668566 No Hit CCGACGGGGGGCCTGGGCCTTTCAGGAATACCACGACGCT 2 0.1899335232668566 No Hit CCGACGGGGGATCATCTTCTGAGATGAGTTTTTGTTCAAT 2 0.1899335232668566 No Hit CCGACGGGGATTGATGCTCAGTTTCAAAGTGAGCCACCAG 2 0.1899335232668566 No Hit CCGACGGGGGCTGAGACCAGTGTTTCTGTTGTGATCCTGA 2 0.1899335232668566 No Hit CCGACGGGGGTTGGTGCCTGTACTTGAACACCTTACAGGA 2 0.1899335232668566 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0949667616334283 0.0 0.0 0.0 0.0 15 0.0949667616334283 0.0 0.0 0.0 0.0 16 0.0949667616334283 0.0 0.0 0.0 0.0 17 0.0949667616334283 0.0 0.0 0.0 0.0 18 0.0949667616334283 0.0 0.0 0.0 0.0 19 0.0949667616334283 0.0 0.0 0.0 0.0 20 0.0949667616334283 0.0 0.0 0.0 0.0 21 0.0949667616334283 0.0 0.0 0.0 0.0 22 0.0949667616334283 0.0 0.0 0.0 0.0 23 0.0949667616334283 0.0 0.0 0.0 0.0 24 0.0949667616334283 0.0 0.0 0.0 0.0 25 0.0949667616334283 0.0 0.0 0.0 0.0 26 0.0949667616334283 0.0 0.0 0.0 0.0 27 0.0949667616334283 0.0 0.0 0.0 0.0 28 0.0949667616334283 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCCTCT 30 5.0846265E-6 34.0 16 TCCTCTT 30 5.0846265E-6 34.0 17 GGGTCAC 25 9.5802614E-5 34.0 7 CTGAGAT 30 5.0846265E-6 34.0 24 GTCACAG 30 5.0846265E-6 34.0 9 ACAGATC 25 9.5802614E-5 34.0 12 CGGGGTC 35 2.539673E-7 34.0 5 GACGGGG 85 0.0 34.0 3 CTCTTCT 40 1.1868906E-8 34.0 19 TTTTTGT 20 0.0017072165 34.0 34 GTTTTTG 20 0.0017072165 34.0 33 TTCTGAG 30 5.0846265E-6 34.0 22 GGTCACA 30 5.0846265E-6 34.0 8 GAGTTTT 25 9.5802614E-5 34.0 31 GATGAGT 30 5.0846265E-6 34.0 28 CCTCTTC 30 5.0846265E-6 34.0 18 GATCCTC 30 5.0846265E-6 34.0 15 TCTGAGA 30 5.0846265E-6 34.0 23 ACGGGGT 35 2.539673E-7 34.0 4 ACGGGGG 40 1.1868906E-8 34.0 4 >>END_MODULE