FastQCFastQC Report
Fri 17 Jun 2016
SRR1525862_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525862_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105477
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG40563.845388094086863No Hit
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG9870.9357490258539776No Hit
GATCTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT9840.9329048038908957No Hit
GATCTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC7180.6807171231642918No Hit
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG4930.46740047593314177No Hit
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG4630.4389582563023219No Hit
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG3420.32424130379134786No Hit
GATCTTGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT2660.2521876807266039No Hit
GATCTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC2430.2303819790096419No Hit
GATCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC2160.20478398134190393No Hit
GATCTTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC2050.1943551674772699No Hit
GATCTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT1940.18392635361263593No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1850.17539368772338992No Hit
GATCTTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT1830.17349753974800194No Hit
GATCTTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC1580.14979569005565194No Hit
GATCTTGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA1550.14695146809256993No Hit
GATCTTGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC1530.14505532011718195No Hit
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC1460.13841880220332395No Hit
GATCTTGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT1450.13747072821562994No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1280.12135347042483194No Hit
GATCTTGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC1180.11187273054789196No Hit
GATCTTGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC1080.10239199067095196No Hit
GATCTTGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG1060.10049584269556397No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACAA203.9704287E-450.4901341
CATGAGG150.00809149350.4901341
CGTCTCA1300.050.4901341
CATTATA150.00809149350.4901341
CCCCATT150.00809149350.4901341
AATTATA309.710402E-750.4901341
CTGTTTC251.9635492E-550.4658340
GCGCATT354.8774382E-850.4415638
CACGTGC150.00812235550.4415639
ATATCAT150.00815330550.3930837
GGCGCAT354.9145456E-850.3930837
TGCACGT150.00815330550.3930837
ATGCTCG1250.050.24820336
TCTAGCG252.0201767E-550.22413635
ACATCCT204.0850163E-450.20009614
TTCTAGC301.0137974E-650.1760834
ACTATTA507.2759576E-1250.1760847
ATATGTC150.00829366850.1760834
CACGATT355.084803E-850.1760847
ATATGCA252.031663E-550.1760834
Produced by FastQC (version 0.11.5)