##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525862_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	105477
Sequences flagged as poor quality	0
Sequence length	56
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.77505048493985	39.0	37.0	40.0	30.0	40.0
2	35.25862510310304	39.0	35.0	40.0	22.0	40.0
3	36.468907913573574	39.0	37.0	40.0	30.0	40.0
4	36.45743621832248	39.0	37.0	40.0	30.0	40.0
5	36.792514007793166	39.0	38.0	40.0	31.0	40.0
6	36.80431752893996	39.0	38.0	40.0	31.0	40.0
7	36.78097594736293	39.0	38.0	40.0	31.0	40.0
8	36.56923310295135	39.0	36.0	40.0	31.0	40.0
9	36.5071721797169	39.0	37.0	40.0	31.0	40.0
10	35.87806820444268	39.0	36.0	40.0	28.0	40.0
11	35.13517638916541	38.0	35.0	40.0	26.0	40.0
12	34.28317073864444	38.0	33.0	40.0	22.0	40.0
13	33.759909743356374	38.0	32.0	40.0	20.0	40.0
14	34.207040397432614	38.0	33.0	40.0	22.0	40.0
15	33.09713018003925	38.0	31.0	39.0	17.0	40.0
16	32.58511334224523	37.0	30.0	39.0	18.0	40.0
17	31.816348587843795	36.0	28.0	39.0	15.0	40.0
18	31.251789489651774	36.0	27.0	39.0	14.0	40.0
19	32.24064013955649	36.0	29.0	39.0	17.0	40.0
20	32.75637342738227	37.0	30.0	39.0	19.0	40.0
21	33.10842174123269	38.0	31.0	39.0	19.0	40.0
22	32.51328725693753	38.0	30.0	39.0	16.0	40.0
23	33.15890668107739	38.0	31.0	39.0	19.0	40.0
24	32.548555609279745	38.0	30.0	39.0	17.0	40.0
25	32.926467381514456	38.0	31.0	39.0	18.0	40.0
26	33.3108165761256	38.0	32.0	39.0	18.0	40.0
27	33.5904699602757	38.0	33.0	39.0	20.0	40.0
28	33.439764119191864	38.0	33.0	39.0	20.0	40.0
29	33.02702958938916	38.0	32.0	39.0	17.0	40.0
30	32.775448676014676	38.0	31.0	39.0	17.0	40.0
31	33.41210880097083	38.0	33.0	39.0	18.0	40.0
32	33.3529584648786	38.0	33.0	39.0	18.0	40.0
33	33.13145993913365	38.0	33.0	39.0	18.0	40.0
34	32.9552698692606	38.0	33.0	39.0	17.0	40.0
35	32.94410155768556	38.0	32.0	39.0	17.0	40.0
36	32.15254510461997	37.0	31.0	39.0	10.0	40.0
37	31.916986641637514	36.0	31.0	39.0	4.0	40.0
38	31.220029011064025	36.0	29.0	38.0	4.0	40.0
39	30.13186761094836	35.0	27.0	38.0	4.0	40.0
40	28.226343183822067	34.0	20.0	38.0	4.0	40.0
41	25.935388757738654	31.0	14.0	38.0	4.0	39.0
42	27.064829299278514	32.0	19.0	38.0	4.0	39.0
43	27.205608805711197	33.0	19.0	38.0	4.0	40.0
44	26.15029816926913	31.0	16.0	38.0	4.0	39.0
45	27.05670430520398	33.0	19.0	38.0	4.0	40.0
46	25.143832304673058	30.0	14.0	36.0	4.0	39.0
47	24.261232306569205	29.0	10.0	36.0	4.0	39.0
48	24.88244830626582	30.0	12.0	36.0	4.0	39.0
49	23.869545019293305	28.0	7.0	36.0	4.0	39.0
50	24.49458175716033	29.0	10.0	36.0	4.0	39.0
51	24.758231652398152	30.0	4.0	36.0	4.0	39.0
52	24.61545170985144	30.0	4.0	36.0	4.0	39.0
53	24.4339524256473	30.0	4.0	36.0	4.0	39.0
54	24.178162063767456	30.0	4.0	36.0	4.0	39.0
55	23.910151028186238	30.0	4.0	36.0	4.0	39.0
56	23.10394683201077	29.0	4.0	36.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	3.0262620712388326
1	2	4.989935981464974
1	3	3.8488594512516627
1	4	3.3892948378999534
1	5	3.2437826327629153
1	6	3.110258812881874
1	7	2.982931721825892
1	8	2.9335976357355023
1	9	3.483270783861215
1	10	3.5487179689210677
1	11	0.583941702840896
1	12	-7.21300218982141
1	13	-17.48952816726552
1	14	-6.964489512474785
1	15	1.0584978174666766
1	16	-0.8778007770262128
1	17	-5.669128583884454
1	18	-3.8514719940825337
1	19	0.5273748718402729
1	20	0.052107841188384185
1	21	5.863924156752422
1	22	6.782722896566323
1	23	5.97107881122313
1	24	6.402220195608365
1	25	5.904623342070803
1	26	5.5150067022633
1	27	5.080393799931699
1	28	5.807112790961725
1	29	5.356026381591658
1	30	4.917686683491041
1	31	4.453402771002569
1	32	4.747523133824593
1	33	4.156622998167602
1	34	4.314344538907239
1	35	4.35833386498059
1	36	3.3571109313241294
1	37	3.3786479798004088
1	38	4.197177507562692
1	39	4.2496054568651935
1	40	-10.535823667764154
1	41	-0.22353035254786136
1	42	-1.2415644727056048
1	43	-9.737688711908609
1	44	-15.08101699722335
1	45	-2.403814876647875
1	46	-5.888212860716656
1	47	-10.86693355661798
1	48	-5.360660736603293
1	49	-14.328222441988915
1	50	-3.875004802931624
1	51	1.9250999416213759
1	52	2.01793663261164
1	53	2.205401627010371
1	54	2.7300971602107076
1	55	2.278376962552194
1	56	5.094672502129541
2	1	3.812220325508278
2	2	5.063180763248653
2	3	4.317739906659632
2	4	3.7773403786969126
2	5	3.7898926896889122
2	6	3.7161411658230463
2	7	3.8117742265697245
2	8	3.742136535166246
2	9	4.236401713652491
2	10	4.252513035334729
2	11	4.503676048191942
2	12	0.8118554951880768
2	13	2.1738494418426413
2	14	5.391867223768095
2	15	5.844266318415446
2	16	5.372483852954812
2	17	7.484571605868869
2	18	5.790653243109119
2	19	6.950714530284298
2	20	6.256661920884778
2	21	6.991438388251471
2	22	7.600369955389855
2	23	7.078668944050449
2	24	7.505825508701342
2	25	7.133465846814634
2	26	6.704570269625734
2	27	6.427262870140424
2	28	6.250566301398159
2	29	7.076329986904749
2	30	6.940457081973015
2	31	5.580347742539569
2	32	5.535189167980192
2	33	5.412979734410488
2	34	5.714154785586555
2	35	6.152451512039416
2	36	6.603410741570805
2	37	7.027224829895285
2	38	7.325640505665156
2	39	7.906910959711492
2	40	6.001557736410419
2	41	11.130169837205461
2	42	9.72010535651641
2	43	8.538592123006005
2	44	-0.7952484962745814
2	45	9.228443187868255
2	46	8.142716930554691
2	47	4.462098701446536
2	48	7.83592370362441
2	49	1.4176978616163964
2	50	8.397670718890009
2	51	10.738382674069193
2	52	10.900289573788111
2	53	11.16270712985667
2	54	10.635979513151884
2	55	10.739667285132839
2	56	11.807765481256677
3	1	3.541632090214158
3	2	5.333768998542773
3	3	4.247151671365515
3	4	3.5596933198733822
3	5	3.6545985720418486
3	6	3.639670577587758
3	7	3.470597755981487
3	8	3.6715482998721285
3	9	3.895225243064253
3	10	4.211336564746496
3	11	4.67426428348606
3	12	5.4059731422469
3	13	5.80326120654852
3	14	5.497749576709275
3	15	0.5266192595919144
3	16	6.601895617660698
3	17	7.849277488221805
3	18	8.190653243109118
3	19	7.350714530284296
3	20	6.274308979708305
3	21	7.020850152957355
3	22	7.659193484801616
3	23	6.896316002873981
3	24	7.641119626348406
3	25	7.021701140932283
3	26	6.633982034331616
3	27	6.027262870140426
3	28	6.344683948456982
3	29	6.676329986904751
3	30	6.528692376090667
3	31	5.262700683716044
3	32	5.117542109156666
3	33	4.73062679323402
3	34	4.8847430208806735
3	35	5.005392688510003
3	36	6.021057800394338
3	37	6.521342476954107
3	38	7.30211109390045
3	39	8.377499195005615
3	40	9.307440089351598
3	41	12.177228660734873
3	42	10.361281827104644
3	43	10.668003887711883
3	44	11.240045621372477
3	45	10.53432554080943
3	46	11.6662463423194
3	47	12.367981054387712
3	48	11.812394291859704
3	49	11.888286096910516
3	50	11.297670718890007
3	51	11.138382674069192
3	52	11.047348397317524
3	53	11.021530659268436
3	54	11.365391277857771
3	55	11.127902579250481
3	56	11.325412540080205
4	1	3.2528946440161732
4	2	4.668417492086533
4	3	3.6319436369322347
4	4	3.2562499913224556
4	5	3.4200218145095675
4	6	3.479771008003823
4	7	3.356967913800716
4	8	3.287688902454626
4	9	3.598022947511886
4	10	3.926688071202733
4	11	4.358410624949471
4	12	5.060994663050344
4	13	5.512084735960286
4	14	5.212025042993346
4	15	5.593548958300669
4	16	7.358351858693691
4	17	8.155733729254802
4	18	8.464971751000078
4	19	7.5406714886774076
4	20	6.834208549292239
4	21	6.115183008050611
4	22	7.679064359980959
4	23	5.966043406030366
4	24	6.33129179277595
4	25	6.145732704777327
4	26	5.503637701476521
4	27	5.4839343192078545
4	28	4.364626559647803
4	29	5.319873745871757
4	30	5.595263394742027
4	31	4.742112448421924
4	32	4.925217862384784
4	33	5.08191804144062
4	34	5.106909448427299
4	35	4.818089962541567
4	36	4.429809593794623
4	37	5.134470167054541
4	38	5.449026445406908
4	39	5.7925637574159445
4	40	-0.7601352334604563
4	41	9.535478302054816
4	42	8.243132616200771
4	43	8.319510343952913
4	44	8.705253655805759
4	45	8.067467578112154
4	46	10.913950789952107
4	47	10.903562116080685
4	48	10.234775927440765
4	49	10.397898722448534
4	50	10.789708021615976
4	51	9.32726359229014
4	52	9.725253705782372
4	53	9.395920903170875
4	54	9.06790203825949
4	55	8.999423382693813
4	56	8.424766915976903
5	1	3.3023189736631906
5	2	5.164971044578579
5	3	4.1363369106786365
5	4	3.263748855130963
5	5	3.361394333824826
5	6	3.3177122290309384
5	7	3.439834146189746
5	8	3.538446363445381
5	9	3.695773288369331
5	10	3.7888301708846015
5	11	4.834512730691024
5	12	5.565198571549054
5	13	5.345935667637306
5	14	5.468008984820344
5	15	6.8524869979916225
5	16	7.468903290294964
5	17	8.27404913601136
5	18	8.864456677526363
5	19	7.724810255530915
5	20	7.185708322053937
5	21	7.040762465708543
5	22	7.528064511473158
5	23	6.537017500864479
5	24	7.198773992442668
5	25	6.8633891204719255
5	26	5.788859637546814
5	27	5.501431668094391
5	28	5.570807441332391
5	29	6.004353370171099
5	30	5.8726456095579636
5	31	5.119441640968507
5	32	4.230512514710192
5	33	4.93197863831988
5	34	4.183829612038874
5	35	4.703675479887426
5	36	5.915796565465584
5	37	5.939537581082714
5	38	6.616652562661869
5	39	7.371945669247484
5	40	9.260088974992808
5	41	12.476169106478384
5	42	10.55342651106518
5	43	10.576516858117436
5	44	11.531605723674264
5	45	10.31167300153285
5	46	11.203111523174346
5	47	12.341601807036596
5	48	10.706219648089153
5	49	12.179371226614574
5	50	11.878671814980617
5	51	11.175430536692502
5	52	10.75706706739425
5	53	11.705381590945457
5	54	10.528854924185861
5	55	10.826002688859546
5	56	10.55486084113976
6	1	3.5427633119336193
6	2	5.039274277577462
6	3	4.107709740747119
6	4	3.475605838727077
6	5	3.4253376368985613
6	6	3.491028043651106
6	7	3.3393006217431775
6	8	3.5419102908223508
6	9	3.890549526623829
6	10	3.805831285711804
6	11	4.807597616819393
6	12	5.848205419773294
6	13	6.063366787242337
6	14	5.647297088021489
6	15	6.799771597450135
6	16	7.175425029425401
6	17	8.229217156396768
6	18	8.987485822294637
6	19	7.626506385487918
6	20	7.1819258726193205
6	21	7.179598267587817
6	22	7.705799819643243
6	23	7.092997752496906
6	24	7.693080410662127
6	25	6.919966600962443
6	26	6.542880978524678
6	27	5.711576595630618
6	28	6.036765396420783
6	29	6.669467241806711
6	30	6.683443507312383
6	31	5.812625268934742
6	32	6.188507418357268
6	33	5.84661472686901
6	34	5.8762965653753945
6	35	5.913462070108796
6	36	6.957483139760853
6	37	7.153544588567986
6	38	7.510783793749624
6	39	8.436247309636077
6	40	5.718601476983572
6	41	12.282507695425668
6	42	11.218898720015655
6	43	11.225545365841597
6	44	11.77435934686267
6	45	11.237417546842611
6	46	13.011042722409893
6	47	13.606970496318333
6	48	12.273706509054271
6	49	12.676823050153349
6	50	12.94480495720072
6	51	11.341022191414595
6	52	11.902552017227027
6	53	11.871530659268437
6	54	12.13137921149276
6	55	11.979410874876429
6	56	12.07767498351912
7	1	3.5872105865133435
7	2	5.68671017501336
7	3	4.18976951320424
7	4	3.913413615938829
7	5	3.771661291169238
7	6	3.7817819916898188
7	7	3.59634765859154
7	8	3.578716200144818
7	9	3.9194169064845923
7	10	4.234554328673255
7	11	5.1166483894463255
7	12	5.944656430131587
7	13	6.291886060463597
7	14	6.196074469580331
7	15	7.087935971707228
7	16	7.677080658564474
7	17	8.850290343695125
7	18	9.27760298989525
7	19	7.687255239283129
7	20	7.409681009314848
7	21	6.956767566280295
7	22	7.855570578685526
7	23	7.1692415969526735
7	24	7.624095863200758
7	25	7.2716816629424095
7	26	6.399817640097098
7	27	6.650870193864613
7	28	6.308415931316354
7	29	6.51107872083541
7	30	6.349261133394911
7	31	5.114629004713311
7	32	5.42848873946442
7	33	5.709239960355173
7	34	5.4556818599924775
7	35	5.031181547099795
7	36	7.333056242155145
7	37	6.990995768734397
7	38	7.960817755372986
7	39	7.796198065282201
7	40	8.128008846655842
7	41	12.109133608144301
7	42	11.729766439492646
7	43	10.910933377388552
7	44	11.450407911984083
7	45	10.966191532239112
7	46	12.693671352058391
7	47	12.618390092175012
7	48	12.031093162136294
7	49	13.048278305714565
7	50	11.902734996256587
7	51	11.491090114661326
7	52	11.858996235260644
7	53	11.518881652645923
7	54	11.296984577429253
7	55	10.89132291427191
7	56	9.648357612148768
8	1	3.626599410475599
8	2	5.4644879527911385
8	3	4.262184351104082
8	4	3.533549529023709
8	5	3.520611643937272
8	6	3.516794760594294
8	7	3.622231742909598
8	8	3.6408293456237573
8	9	3.907643543717846
8	10	4.200879048406627
8	11	5.036355786754029
8	12	5.675254187998533
8	13	6.2261370235419875
8	14	5.363109053833455
8	15	6.880214030833745
8	16	7.516928297399257
8	17	8.512022586261022
8	18	8.594574811736571
8	19	7.815420412637234
8	20	7.310256692126611
8	21	6.939150806552124
8	22	7.776186948853901
8	23	7.219191819867447
8	24	7.67183858059677
8	25	6.8798710755728045
8	26	5.863393799037496
8	27	5.329223654454147
8	28	5.39108917721515
8	29	5.729924758146584
8	30	5.597973421842298
8	31	4.975772579140877
8	32	4.6913983183069945
8	33	4.8084045710117955
8	34	5.214154785586555
8	35	5.167484191777977
8	36	5.806025120655775
8	37	6.1383359410063925
8	38	6.204725472985423
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115	42	7.085538952635265
115	43	6.258786180010247
115	44	-0.7041629899070863
115	45	6.308855559002275
115	46	1.7564828492933202
115	47	-8.350272432574082
115	48	0.8892893797917836
115	49	-2.716383399754129
115	50	1.3452146849300313
115	51	2.963610084621042
115	52	3.1210294161167944
115	53	3.7400873602993663
115	54	3.134220871550916
115	55	3.309770377916344
115	56	5.60800805251198
116	1	3.3837889529592573
116	2	5.161710175013361
116	3	3.7711712792086516
116	4	3.1454776335988726
116	5	3.2496966112575336
116	6	3.1769254795485367
116	7	3.0764801089226665
116	8	3.1720384959505594
116	9	3.535421321495626
116	10	3.3250620549425705
116	11	3.390930950152722
116	12	3.252051573619447
116	13	4.0537514026269506
116	14	4.465886831611236
116	15	-5.5694591717806325
116	16	3.833758362758733
116	17	5.756140233319844
116	18	5.2009473607561745
116	19	5.325224334205867
116	20	4.670387411080856
116	21	6.034085447074997
116	22	6.557722896566322
116	23	5.94876698326614
116	24	6.084747077328792
116	25	6.331505062500906
116	26	4.5217271323708275
116	27	4.294909928963953
116	28	4.641252575907963
116	29	5.669467241806711
116	30	4.900751199620075
116	31	4.125445781755257
116	32	3.6077381875880405
116	33	4.361999342253629
116	34	4.387194001272828
116	35	3.8333338649805917
116	36	5.590175447453163
116	37	5.337518947542346
116	38	6.136424819390648
116	39	7.007401155789925
116	40	8.879498912881008
116	41	8.460071797989773
116	42	5.9406935918105255
116	43	1.5268274171236484
116	44	-0.1423073198039937
116	45	5.253443187868253
116	46	0.11232477369194527
116	47	-5.796234631886797
116	48	-0.7327037473559805
116	49	-2.282792334462034
116	50	-0.22634888895312955
116	51	4.30897090936331
116	52	3.8262699659449737
116	53	3.7215306592684385
116	54	3.6884304935440433
116	55	4.156333951799503
116	56	6.368059598903734
117	1	2.7706683855833703
117	2	4.463305919694214
117	3	3.144398229563265
117	4	2.8218960733151874
117	5	2.5238100864348425
117	6	2.7017481745839973
117	7	2.789246066369472
117	8	2.904839914390273
117	9	3.082584442063002
117	10	3.1796719840205867
117	11	1.0806472622094603
117	12	-6.898657646238707
117	13	-6.206000370422693
117	14	-0.5364181329277713
117	15	-7.451728675326734
117	16	-6.691596247170342
117	17	0.962168602114172
117	18	-2.527244128605524
117	19	0.598273979595934
117	20	0.35691223377589054
117	21	4.864404596011173
117	22	5.325276088055684
117	23	4.564901735039186
117	24	5.446626509952907
117	25	5.064129175976088
117	26	5.61871294797367
117	27	5.002356737474592
117	28	5.175472434063991
117	29	4.635779298544303
117	30	5.3863894974924165
117	31	4.077218831400646
117	32	3.3495821592192456
117	33	4.018736930906105
117	34	4.308647901982052
117	35	4.075355141576335
117	36	5.674749915538264
117	37	5.592306181584899
117	38	6.619758152723982
117	39	7.41892597848496
117	40	8.750243593732069
117	41	7.920532790897575
117	42	7.2396297620232914
117	43	-3.2284917318125217
117	44	1.208756509983239
117	45	7.702911272974635
117	46	-0.6256184887194003
117	47	-0.4485395964258032
117	48	-0.1227746693417977
117	49	-0.2858065188591965
117	50	1.9834028840965168
117	51	5.53237516468246
117	52	6.2413408879307894
117	53	5.249190233736524
117	54	6.276905670849008
117	55	6.405624731941348
117	56	9.347314918052671
118	1	1.9548859993727632
118	2	4.117089921848802
118	3	2.87507423279515
118	4	2.0310261568056234
118	5	2.313198720962177
118	6	2.244436028071746
118	7	2.044834539302414
118	8	2.2987262596636384
118	9	2.4744508573606083
118	10	2.6457371604278066
118	11	-0.3105458430962216
118	12	-7.208918890515573
118	13	-8.288548175432119
118	14	-8.892868442650368
118	15	-10.93570389751903
118	16	-11.00495471741004
118	17	-1.6284589227982984
118	18	-7.34114124683876
118	19	-1.7802608978616448
118	20	-1.8481779897630268
118	21	2.98250316859399
118	22	3.782722896566323
118	23	2.965117194236605
118	24	3.7131226047549575
118	25	3.22222236207897
118	26	3.901368482581802
118	27	4.000606131495601
118	28	3.354438229916404
118	29	4.569256271342578
118	30	3.7934727186074184
118	31	2.226289663611798
118	32	2.806894305731497
118	33	2.7826744477388594
118	34	3.2573416805977224
118	35	2.983650320676791
118	36	3.863276713275944
118	37	3.3384683146309477
118	38	4.600770810951829
118	39	5.46700031190807
118	40	7.572220431868349
118	41	5.288025384487664
118	42	4.793225237380145
118	43	-1.2706409373067302
118	44	-2.465092129930575
118	45	4.476544453691037
118	46	-2.389152019556999
118	47	-3.507521551718021
118	48	-2.563927376047964
118	49	-1.9461889589346058
118	50	-0.2884796906408944
118	51	4.3659329346797655
118	52	5.195151822485059
118	53	3.5898850896481846
118	54	4.380730071603114
118	55	4.5637179180442295
118	56	6.5063507381442385
119	1	2.6829298464300493
119	2	4.71763801006491
119	3	3.425380901201784
119	4	2.740408905076535
119	5	2.6076003913262653
119	6	2.561805204634453
119	7	2.1641089749020495
119	8	2.7272790457787366
119	9	3.1527752733856644
119	10	3.2872613676573508
119	11	1.4227178917334768
119	12	-7.066676948717319
119	13	-1.6090492846582691
119	14	-5.298975711344092
119	15	-9.127534773155205
119	16	-8.296224455110682
119	17	0.6274460752442437
119	18	-4.437970165017017
119	19	0.4661178049962409
119	20	0.2418650743110966
119	21	5.449291632642012
119	22	6.3084960924426134
119	23	5.371876948901878
119	24	5.20940343471711
119	25	5.174460388961389
119	26	5.904630912439558
119	27	5.895425392881478
119	28	5.509207902368445
119	29	6.407439750397781
119	30	6.355132642919045
119	31	5.265308324710585
119	32	5.303958118859512
119	33	5.321105871463249
119	34	5.5767988122693914
119	35	4.927406029929042
119	36	6.355381633020173
119	37	6.448085957851621
119	38	7.274397327981717
119	39	7.994600468504704
119	40	10.092643242777914
119	41	7.749332966374652
119	42	6.518528643356916
119	43	-2.0401828921547036
119	44	-1.796946495061725
119	45	5.43256689920846
119	46	-0.85176457338709
119	47	-2.226561092367895
119	48	-4.677720929486565
119	49	-1.0256617502695917
119	50	1.3349054065795158
119	51	6.623403899054033
119	52	7.028245910962156
119	53	6.585448185041631
119	54	5.278550768458132
119	55	6.309770377916344
119	56	8.535843104058372
120	1	2.678886992174945
120	2	4.294553312268263
120	3	2.680485004698852
120	4	2.4420462610498532
120	5	2.3996966112575393
120	6	2.373003910921085
120	7	2.32157814813835
120	8	2.653901241048594
120	9	2.410911517574057
120	10	2.874081662785713
120	11	-0.006127873376684079
120	12	-6.586183720498198
120	13	-5.433993695412262
120	14	-5.0846033644671955
120	15	-0.3420081913884765
120	16	-10.731437715672637
120	17	-0.6154283941311363
120	18	-7.689738913753626
120	19	-1.2434031167745268
120	20	-0.45118121637012365
120	21	2.599281525506374
120	22	4.968997406370246
120	23	3.8629826695406493
120	24	4.629354920466049
120	25	3.7099364350499293
120	26	5.412413406880631
120	27	4.750792281905127
120	28	4.59370355630012
120	29	5.076329986904749
120	30	5.3365355133455665
120	31	3.8940732327356464
120	32	3.521463677784112
120	33	3.765920910881075
120	34	4.449448903233613
120	35	3.683824061059024
120	36	5.387724467061005
120	37	5.448793457346266
120	38	5.668777760567117
120	39	6.561812920495807
120	40	8.056459697194729
120	41	-4.516888986323952
120	42	-2.7524436630914373
120	43	-6.639839249543019
120	44	-13.446228888431445
120	45	-1.6950862238964532
120	46	-6.800420324347268
120	47	-5.310450318161305
120	48	-3.0660370806893162
120	49	-13.737204099167915
120	50	-2.357231241894304
120	51	2.9266179681868394
120	52	2.7434268286900725
120	53	3.288197325935105
120	54	3.32029323864208
120	55	3.080843755721073
120	56	7.619530187139027
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	2057.0
5	159.0
6	285.0
7	454.0
8	609.0
9	634.0
10	538.0
11	413.0
12	348.0
13	289.0
14	339.0
15	410.0
16	489.0
17	560.0
18	715.0
19	910.0
20	1140.0
21	1508.0
22	1897.0
23	2239.0
24	2726.0
25	2984.0
26	3222.0
27	3362.0
28	3530.0
29	3716.0
30	4182.0
31	4579.0
32	5362.0
33	6084.0
34	6548.0
35	7195.0
36	7683.0
37	8591.0
38	9404.0
39	10312.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.66912217829478	0.23227812698502992	0.07015747508935598	0.028442219630819988
8	99.5752628535131	0.2882144922589759	0.08911895484323597	0.04740369938469998
9	97.66293114890874	0.733830138233119	0.9680110738191403	0.6352276390390049
10	47.716563800639	22.00479725437773	21.607554253533944	8.671084691449321
11	30.52798240374679	31.482692909354647	16.397887691155418	21.59143699574315
12	27.15473515553154	20.814016325834068	18.848658949344408	33.18258956928999
13	21.56583899807541	15.163495359177832	21.433108639798252	41.837557002948515
14	20.749547294670876	26.497719882059595	31.231453302615737	21.521279520653792
15	37.371870594063175	17.741874023549137	21.701025035247497	23.18523034714019
16	33.86549041927528	25.189717321191424	21.234111174350218	19.710681085183076
17	25.424130638874924	17.987501303923146	36.68338849323383	19.904979563968098
18	22.214321605658107	17.99823658238289	30.550736179451444	29.236705632507558
19	32.16541781276677	20.099592146447883	20.507445698567768	27.227544342217584
20	23.686533587721048	29.81936573929056	25.709295592827786	20.784805080160606
21	21.889132227879063	19.472491633247056	31.114840202129372	27.5235359367445
22	20.801691363994046	18.0759786493738	29.216796078765988	31.905533907866168
23	20.80263943798174	20.322914000208574	26.414289371142523	32.46015719066716
24	20.497942620930274	20.36615658835353	30.618920302633825	28.51698048808237
25	32.13828546504461	20.91349572835971	25.58148355347373	21.36673525312195
26	30.12066479471807	20.420050087273278	19.468581619488504	29.990703498520148
27	22.426732194191707	25.450422671511657	18.994127190444114	33.12871794385253
28	25.73097197466722	32.64249687132617	19.261253744927757	22.365277409078843
29	37.51756751623808	23.430318684240515	18.51806130588369	20.53405249363771
30	27.59601976835735	20.29386934292029	31.978448316749038	20.131662571973326
31	27.88397716713129	32.33012838747606	20.32959739052929	19.456297054863363
32	37.66737472973485	23.860144900049313	18.92899138944733	19.5434889807685
33	28.338459201883282	23.456544025515434	19.313133614306867	28.891863158294417
34	22.44112813595387	29.72138575676112	27.997264203816812	19.84022190346819
35	22.27950741067783	23.094159759212314	36.7998784667825	17.82645436332735
36	26.873500203924845	30.762299513425845	22.555035995105804	19.809164287543513
37	26.026928993296238	21.761589152439374	32.527489222705434	19.683992631558958
38	35.77870059772125	22.58987199832752	21.979797211901207	19.651630192050025
39	25.97747858946849	21.711513264085376	19.783141224055754	32.52786692239039
40	21.92657041782197	17.317143130318385	30.14341715269146	30.61286929916818
41	19.102049419858005	15.584788878136646	26.927019957651073	38.386141744354276
42	28.99669973274493	24.101461818666007	22.54643675755875	24.355401691030313
43	18.417650525675395	20.60875682047187	33.344740394304075	27.62885225954866
44	20.346728358038444	22.328849284551453	24.765087253305914	32.55933510410419
45	23.570831274468812	20.983313694933294	33.233874339579614	22.211980691018283
46	19.444919847110505	25.263848479662276	23.03801319717801	32.25321847604921
47	17.969338072052672	28.688626120761118	22.454288191632283	30.887747615553923
48	23.750343898528588	29.483250955800738	22.562589532203134	24.20381561346754
49	19.62776726899423	29.79572825869985	21.697099892588614	28.87940457971731
50	28.7445854548218	24.369252982749448	22.87027889657269	24.01588266585607
51	20.46189552182019	32.22425141726591	24.181980748012023	23.131872312901873
52	20.926388585433507	27.35468181515926	31.334010962391588	20.384918637015645
53	20.823707253073216	25.654335776693955	31.352975290683855	22.168981679548978
54	21.0376748783455	29.34819799270073	26.634732360097324	22.979394768856448
55	20.057810613191847	28.891994941571348	26.615702047142275	24.43449239809453
56	27.850808406284756	20.96533500622582	27.374604351421482	23.809252236067945
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	3.5
5	7.0
6	7.5
7	8.0
8	66.0
9	124.0
10	169.5
11	215.0
12	239.0
13	252.0
14	241.0
15	241.5
16	242.0
17	238.5
18	235.0
19	247.5
20	260.0
21	253.5
22	281.5
23	316.0
24	366.0
25	416.0
26	434.5
27	453.0
28	530.5
29	706.5
30	805.0
31	904.5
32	1004.0
33	1164.5
34	1325.0
35	1412.0
36	1499.0
37	1531.0
38	1751.5
39	1940.0
40	2226.0
41	2512.0
42	3340.5
43	4169.0
44	7546.5
45	10924.0
46	10109.0
47	7743.5
48	6193.0
49	5904.5
50	5616.0
51	5704.5
52	5793.0
53	5927.5
54	5939.5
55	5817.0
56	6017.5
57	6218.0
58	6364.5
59	6511.0
60	6118.5
61	5726.0
62	5190.5
63	4381.5
64	4108.0
65	3514.5
66	2921.0
67	2636.0
68	2351.0
69	2045.0
70	1739.0
71	1551.5
72	1005.0
73	646.0
74	505.5
75	365.0
76	332.0
77	299.0
78	307.0
79	194.5
80	74.0
81	50.5
82	27.0
83	16.5
84	6.0
85	3.0
86	0.0
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.002844221963081999
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.4787773637854698
16	0.05404021729855798
17	0.02464992368004399
18	0.0
19	0.044559477421617984
20	0.11945732244944396
21	0.0
22	0.0
23	0.0
24	0.002844221963081999
25	0.013273035827715995
26	0.05783251324933397
27	0.07205362306474397
28	9.480739876939995E-4
29	0.16022450392028595
30	0.05309214331086398
31	0.014221109815409994
32	0.02370184969234999
33	0.12230154441252596
34	0.19530324146496392
35	0.14789954208026393
36	0.04361140343392398
37	0.15453605999412195
38	0.2313300529973359
39	0.14695146809256993
40	0.8409416270845778
41	1.0485698303895636
42	0.3166567118897959
43	0.2645126425666259
44	0.4152564066099719
45	0.22848583103425393
46	0.2872664182712819
47	0.49868691752704386
48	0.06446903116319197
49	0.2597722726281559
50	0.1943551674772699
51	0.3270855257544299
52	0.19719938944035192
53	0.2787337523820359
54	0.2474473107881339
55	0.2901106402343639
56	0.25597997667737993
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	105477.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.80792969083306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.36192827848497	59.03277491775458
2	6.749258518171626	9.018079770945324
3	2.003774816580811	4.016041411871782
4	0.8159847872053585	2.180570171696199
5	0.5066201200561965	1.6923120680337893
6	0.3192983949934012	1.2798998833868995
7	0.24692409212823024	1.1547541170112914
8	0.16461606141882015	0.8798126605800316
9	0.11494742219762442	0.6911459370289258
>10	0.6400158939645507	7.767570181176938
>50	0.04257311933245349	1.9975918920712572
>100	0.028382079554968992	3.8946879414469504
>500	0.004257311933245349	2.549370952909165
>1k	0.0014191039777484496	3.845388094086863
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	4056	3.845388094086863	No Hit
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	987	0.9357490258539776	No Hit
GATCTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	984	0.9329048038908957	No Hit
GATCTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	718	0.6807171231642918	No Hit
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	493	0.46740047593314177	No Hit
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG	463	0.4389582563023219	No Hit
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG	342	0.32424130379134786	No Hit
GATCTTGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	266	0.2521876807266039	No Hit
GATCTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	243	0.2303819790096419	No Hit
GATCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	216	0.20478398134190393	No Hit
GATCTTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	205	0.1943551674772699	No Hit
GATCTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	194	0.18392635361263593	No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	185	0.17539368772338992	No Hit
GATCTTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT	183	0.17349753974800194	No Hit
GATCTTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC	158	0.14979569005565194	No Hit
GATCTTGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA	155	0.14695146809256993	No Hit
GATCTTGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC	153	0.14505532011718195	No Hit
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC	146	0.13841880220332395	No Hit
GATCTTGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT	145	0.13747072821562994	No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	128	0.12135347042483194	No Hit
GATCTTGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC	118	0.11187273054789196	No Hit
GATCTTGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC	108	0.10239199067095196	No Hit
GATCTTGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG	106	0.10049584269556397	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	9.480739876939996E-4	0.0	0.0	0.0	0.0
10	0.006636517913857998	0.0	0.0	0.0	0.0
11	0.012324961840021995	0.0	0.0	0.0	0.0
12	0.012324961840021995	0.0	0.0	0.0	0.0
13	0.015169183803103994	0.0	0.0	0.0	0.0
14	0.03507873754467799	0.0	0.0	0.0	0.0
15	0.036026811532371986	0.0	0.0	0.0	0.0
16	0.039819107483147986	0.0	0.0	0.0	0.0
17	0.04266332944622998	0.0	0.0	0.0	0.0
18	0.048351773372393984	0.0	0.0	0.0	0.0
19	0.05498829128625198	0.0	0.0	0.0	0.0
20	0.06257288318780398	0.0	0.0	0.0	0.0
21	0.06920940110166197	0.0	0.0	0.0	0.0
22	0.07679399300321398	0.0	0.0	0.0	0.0
23	0.08248243692937797	0.0	0.0	0.0	0.0
24	0.08817088085554196	0.0	0.0	0.0	0.0
25	0.09670354674478797	0.0	0.0	0.0	0.0
26	0.10334006465864597	0.0	0.0	0.0	0.0
27	0.11471695251097395	0.0	0.0	0.0	0.0
28	0.12230154441252596	0.0	0.0	0.0	0.0
29	0.12704191435099596	0.0	0.0	0.0	0.0
30	0.12988613631407794	0.0	0.0	0.0	0.0
31	0.13462650625254796	0.0	0.0	0.0	0.0
32	0.13652265422793594	0.0	0.0	0.0	0.0
33	0.14031495017871196	0.0	0.0	0.0	0.0
34	0.14410724612948794	0.0	0.0	0.0	0.0
35	0.14695146809256995	0.0	0.0	0.0	0.0
36	0.14884761606795793	0.0	0.0	0.0	0.0
37	0.15927642993259195	0.0	0.0	0.0	0.0
38	0.16401679987106194	0.0	0.0	0.0	0.0
39	0.16591294784644994	0.0	0.0	0.0	0.0
40	0.16875716980953193	0.0	0.0	0.0	0.0
41	0.16875716980953193	0.0	0.0	0.0	0.0
42	0.17160139177261394	0.0	0.0	0.0	0.0
43	0.18582250158802394	0.0	0.0	0.0	0.0
44	0.19625131545265792	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACAA	20	3.9704287E-4	50.49013	41
CATGAGG	15	0.008091493	50.49013	41
CGTCTCA	130	0.0	50.49013	41
CATTATA	15	0.008091493	50.49013	41
CCCCATT	15	0.008091493	50.49013	41
AATTATA	30	9.710402E-7	50.49013	41
CTGTTTC	25	1.9635492E-5	50.46583	40
GCGCATT	35	4.8774382E-8	50.44156	38
CACGTGC	15	0.008122355	50.44156	39
ATATCAT	15	0.008153305	50.39308	37
GGCGCAT	35	4.9145456E-8	50.39308	37
TGCACGT	15	0.008153305	50.39308	37
ATGCTCG	125	0.0	50.248203	36
TCTAGCG	25	2.0201767E-5	50.224136	35
ACATCCT	20	4.0850163E-4	50.200096	14
TTCTAGC	30	1.0137974E-6	50.17608	34
ACTATTA	50	7.2759576E-12	50.17608	47
ATATGTC	15	0.008293668	50.17608	34
CACGATT	35	5.084803E-8	50.17608	47
ATATGCA	25	2.031663E-5	50.17608	34
>>END_MODULE