FastQCFastQC Report
Fri 17 Jun 2016
SRR1525861_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525861_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9524
Sequences flagged as poor quality0
Sequence length40
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT590.6194876102477951No Hit
GATCTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT190.19949601007979842No Hit
GATCTTGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT170.1784964300713986No Hit
GATCTTGGGGCTTAATTCTCATTCTCTCTCTCTCTCTCTC150.15749685006299874No Hit
GATCTTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT130.1364972700545989No Hit
GATCTTGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT120.125997480050399No Hit
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT120.125997480050399No Hit
GATCTTGGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAA110.11549769004619909No Hit
GATCTTGGGCTCGTCTTAATCAGCAACATCTTTTGATACA100.10499790004199916No Hit
GATCTTGGGTACCTGGTTGATCCTGCCCGTAGCCTCTGCT100.10499790004199916No Hit
GATCTTGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.10499790004199916No Hit
GATCTTGGGGAGTGCAGTGCTGATCTTGGGACACGCAGGG100.10499790004199916No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTGA200.0025441134.2872352
CTTGGGT1250.034.2872354
TTGGGTG503.074092E-1034.2872355
TTGGGTA552.1827873E-1134.2872355
TGGGGGC200.0025441134.2872356
TGGGGCT200.0025441134.2872356
TTGGGCA406.2378604E-834.2872355
TGGGGAG552.1827873E-1134.2872356
TGGGGAC406.2378604E-834.2872356
TGGGTGG251.789022E-434.2872356
TGGGTGC200.0025441134.2872356
TGGGCAG200.0025441134.2872356
TGGGTAG200.0025441134.2872356
GGGAAGC454.3837645E-934.2872357
TGGGATC200.0025441134.2872356
TGGGAAT251.789022E-434.2872356
GGGGAGT358.8644447E-734.2872357
GGGGAGC200.0025441134.2872357
GGGTGGT251.789022E-434.2872357
GGGTACC301.2591205E-534.287237
Produced by FastQC (version 0.11.5)