##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525861_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9524
Sequences flagged as poor quality	0
Sequence length	40
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.340193196136077	30.0	28.0	33.0	19.0	35.0
2	19.186686266274673	20.0	12.0	26.0	4.0	31.0
3	16.568143637127257	15.0	12.0	17.0	4.0	33.0
4	20.458420831583368	21.0	12.0	29.0	4.0	33.0
5	23.35720285594288	26.0	18.0	31.0	4.0	34.0
6	23.7814993700126	27.0	18.0	31.0	4.0	35.0
7	22.998740025199496	26.0	16.0	31.0	4.0	34.0
8	23.96157076858463	28.0	18.0	32.0	4.0	35.0
9	22.85027299454011	27.0	15.0	31.0	4.0	34.0
10	19.988870222595548	22.0	10.0	29.0	4.0	33.0
11	24.373687526249476	29.0	15.0	35.0	4.0	38.0
12	23.62620747585048	28.0	13.0	34.0	4.0	37.0
13	24.62505249895002	30.0	15.0	35.0	4.0	38.0
14	24.886287274254514	30.0	15.0	35.0	4.0	38.0
15	23.42482150356993	28.0	11.0	35.0	4.0	38.0
16	21.093238135237296	24.0	4.0	33.0	4.0	36.0
17	21.673036539269216	26.0	4.0	33.0	4.0	36.0
18	21.078958420831583	25.0	4.0	33.0	4.0	36.0
19	18.505039899202014	19.0	4.0	30.0	4.0	35.0
20	20.00314993700126	23.0	4.0	32.0	4.0	36.0
21	17.517009659806803	17.0	4.0	30.0	4.0	35.0
22	17.34932801343973	17.0	4.0	30.0	4.0	34.0
23	17.241915161696767	17.0	4.0	30.0	4.0	34.0
24	16.964510709785806	16.0	4.0	30.0	4.0	34.0
25	16.6817513649727	14.0	4.0	30.0	4.0	34.0
26	14.726270474590509	4.0	4.0	28.0	4.0	33.0
27	14.610982780344393	4.0	4.0	28.0	4.0	33.0
28	14.279609407811844	4.0	4.0	28.0	4.0	33.0
29	13.539584208315834	4.0	4.0	26.0	4.0	33.0
30	12.550188996220076	4.0	4.0	23.0	4.0	32.0
31	12.21955060898782	4.0	4.0	24.0	4.0	33.0
32	11.519844603107938	4.0	4.0	21.0	4.0	32.0
33	11.16747165056699	4.0	4.0	19.0	4.0	31.0
34	10.771629567408652	4.0	4.0	17.0	4.0	31.0
35	10.297984040319193	4.0	4.0	15.0	4.0	31.0
36	10.271419571608568	4.0	4.0	13.0	4.0	32.0
37	10.169991600167997	4.0	4.0	11.0	4.0	32.0
38	9.904661906761865	4.0	4.0	4.0	4.0	32.0
39	9.610142797144057	4.0	4.0	4.0	4.0	31.0
40	9.465770684586309	4.0	4.0	4.0	4.0	31.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	6.192094249468745
1	2	12.901502566681398
1	3	17.65646689571711
1	4	11.914063681944583
1	5	13.877294398845816
1	6	11.544280533057766
1	7	11.799515291256363
1	8	3.989102501820355
1	9	4.237755385471669
1	10	5.789757220504608
1	11	-7.936491210119744
1	12	-6.190577882727585
1	13	-9.511627019188715
1	14	-4.467293448646636
1	15	-6.39854694285728
1	16	-2.632089794371435
1	17	-4.453270631515153
1	18	-4.77287363830505
1	19	-6.208187803051576
1	20	-4.867544708410804
1	21	-2.9632797985721844
1	22	-4.540260908256723
1	23	-1.6030903804456198
1	24	-5.8544354442028315
1	25	-1.1873174720719888
1	26	-4.544017016086368
1	27	-2.5501262794370767
1	28	-2.6030059781556414
1	29	-2.0594082106496785
1	30	-1.322232333513849
1	31	0.32748244103159685
1	32	0.10913916887478692
1	33	1.8308955860959184
1	34	1.5853708295956093
1	35	1.4205708974133096
1	36	0.7766734295226048
1	37	-0.8184355616936756
1	38	0.017574040837615712
1	39	0.553931252268125
1	40	0.9844766752704857
2	1	4.317094249468745
2	2	10.276502566681398
2	3	-1.5310331042828906
2	4	14.851563681944583
2	5	10.127294398845816
2	6	-5.143219466942234
2	7	5.362015291256363
2	8	-16.948397498179645
2	9	-15.262244614528331
2	10	3.852257220504608
2	11	-0.686491210119744
2	12	-7.690577882727585
2	13	-7.949127019188715
2	14	-14.842293448646636
2	15	-12.71104694285728
2	16	12.992910205628565
2	17	9.921729368484847
2	18	7.91462636169495
2	19	-6.645687803051576
2	20	11.569955291589196
2	21	-12.338279798572184
2	22	-12.102760908256723
2	23	-11.79059038044562
2	24	-11.979435444202831
2	25	-12.062317472071989
2	26	-10.169017016086368
2	27	-9.800126279437077
2	28	-9.728005978155641
2	29	-9.121908210649678
2	30	-8.072232333513849
2	31	-7.797517558968403
2	32	-7.078360831125213
2	33	-6.981604413904082
2	34	-6.414629170404391
2	35	-6.3919291025866904
2	36	-6.223326570477395
2	37	-6.318435561693676
2	38	-6.107425959162384
2	39	-5.821068747731875
2	40	-5.578023324729514
3	1	6.317094249468745
3	2	-14.723497433318602
3	3	-12.53103310428289
3	4	-16.148436318055417
3	5	-18.872705601154184
3	6	-19.143219466942234
3	7	-18.637984708743637
3	8	-18.948397498179645
3	9	-18.26224461452833
3	10	-15.147742779495392
3	11	-19.686491210119744
3	12	-18.690577882727585
3	13	-19.949127019188715
3	14	-19.842293448646636
3	15	-18.71104694285728
3	16	-16.007089794371435
3	17	-17.078270631515153
3	18	-16.08537363830505
3	19	-13.645687803051576
3	20	-15.430044708410804
3	21	-12.338279798572184
3	22	-12.102760908256723
3	23	-11.79059038044562
3	24	-11.979435444202831
3	25	-12.062317472071989
3	26	-10.169017016086368
3	27	-9.800126279437077
3	28	-9.728005978155641
3	29	-9.121908210649678
3	30	-8.072232333513849
3	31	-7.797517558968403
3	32	-7.078360831125213
3	33	-6.981604413904082
3	34	-6.414629170404391
3	35	-6.3919291025866904
3	36	-6.223326570477395
3	37	-6.318435561693676
3	38	-6.107425959162384
3	39	-5.821068747731875
3	40	-5.578023324729514
5	1	3.459951392325891
5	2	-0.4377831476043177
5	3	16.611824038574255
5	4	-5.291293460912559
5	5	8.127294398845816
5	6	1.1424948187720503
5	7	-8.923698994457924
5	8	-9.091254641036787
5	9	-10.119387471671189
5	10	4.852257220504608
5	11	2.1706516470231136
5	12	-5.833435025584727
5	13	-5.949127019188715
5	14	-3.413722020075209
5	15	-1.8539040857144222
5	16	6.421481634199992
5	17	4.0645865113419894
5	18	2.3431977902663768
5	19	3.782883625519851
5	20	1.4270981487320533
5	21	13.233148772856389
5	22	9.611524806028992
5	23	6.9236953338400955
5	24	2.4491359843685956
5	25	7.6519682422137265
5	26	15.116697269627917
5	27	10.342730863420066
5	28	9.271994021844359
5	29	8.592377503636037
5	30	9.213481952200436
5	31	7.916768155317312
5	32	0.6359248831605004
5	33	-0.5530329853326528
5	34	6.013942258167036
5	35	-5.249071959729547
5	36	-5.3661837133345385
5	37	-2.604149847407961
5	38	-1.964568816305242
5	39	-5.821068747731875
5	40	-5.578023324729514
6	1	-0.4829057505312555
6	2	-0.2234974333186024
6	3	-4.13103310428289
6	4	-0.8484363180554162
6	5	0.32729439884581524
6	6	2.756780533057764
6	7	-5.837984708743637
6	8	-7.648397498179644
6	9	-0.7622446145283313
6	10	4.852257220504608
6	11	3.6135087898802567
6	12	4.309422117272415
6	13	0.750872980811284
6	14	2.3577065513533633
6	15	3.9889530571427194
6	16	5.5929102056285664
6	17	4.621729368484846
6	18	2.3146263616949483
6	19	3.2543121969484226
6	20	-2.5300447084108058
6	21	6.161720201427816
6	22	8.497239091743278
6	23	5.20940961955438
6	24	4.820564555797169
6	25	3.737682527928012
6	26	7.230982983913631
6	27	4.899873720562923
6	28	5.771994021844359
6	29	8.27809178935032
6	30	10.527767666486152
6	31	5.502482441031598
6	32	6.3216391688747855
6	33	3.618395586095918
6	34	4.685370829595609
6	35	1.4080708974133103
6	36	3.976673429522604
6	37	2.0815644383063248
6	38	5.792574040837616
6	39	1.4789312522681257
6	40	1.621976675270485
7	1	-0.22836029598580154
7	2	4.458320748499581
7	3	-0.3492149224647072
7	4	5.215200045580946
7	5	3.536385307936726
7	6	-1.7341285578513244
7	7	-0.0925301632890907
7	8	0.4152388654567183
7	9	1.3286644763807587
7	10	1.1704390386864247
7	11	7.858963335334803
7	12	5.173058480908779
7	13	7.823600253538558
7	14	7.384979278626091
7	15	10.743498511688173
7	16	5.629273841992202
7	17	6.921729368484847
7	18	4.4600809071494965
7	19	6.308857651493877
7	20	9.251773473407379
7	21	6.616265655973269
7	22	6.306330000834187
7	23	6.1639550740998335
7	24	7.975110010342622
7	25	4.346773437018921
7	26	-1.0781079251772763
7	27	-0.66376264307344
7	28	0.544721294571632
7	29	-0.6673627561042235
7	30	-1.8904141516956674
7	31	-3.388426649877495
7	32	-2.2147244674888498
7	33	-1.6179680502677183
7	34	-2.323720079495299
7	35	-4.210110920768509
7	36	-3.0415083886592136
7	37	-3.045708288966403
7	38	-2.789244140980566
7	39	-3.5483414750046025
7	40	-1.5780233247295143
8	1	-2.0829057505312534
8	2	-1.8234974333186038
8	3	-2.1310331042828903
8	4	-4.0484363180554155
8	5	-0.27270560115418263
8	6	5.356780533057766
8	7	1.1620152912563633
8	8	-1.7483974981796457
8	9	1.0377553854716695
8	10	-5.347742779495391
8	11	1.813508789880256
8	12	4.809422117272415
8	13	6.950872980811283
8	14	4.957706551353365
8	15	-1.3110469428572813
8	16	6.892910205628564
8	17	-0.17827063151515432
8	18	6.41462636169495
8	19	8.554312196948423
8	20	6.869955291589196
8	21	9.561720201427814
8	22	10.397239091743277
8	23	9.20940961955438
8	24	10.12056455579717
8	25	3.937682527928011
8	26	7.230982983913631
8	27	7.899873720562923
8	28	7.671994021844357
8	29	3.978091789350323
8	30	4.927767666486151
8	31	7.902482441031596
8	32	5.721639168874788
8	33	7.918395586095917
8	34	0.1853708295956089
8	35	8.008070897413308
8	36	6.676673429522603
8	37	2.8815644383063237
8	38	6.292574040837614
8	39	10.078931252268124
8	40	10.821976675270484
9	1	3.317094249468745
9	2	1.6098359000147298
9	3	-0.19769977094955848
9	4	3.1848970152779152
9	5	6.460627732179148
9	6	8.856780533057766
9	7	8.695348624589695
9	8	7.051602501820355
9	9	10.071088718805004
9	10	11.518923887171276
9	11	1.9801754565469238
9	12	12.97608878393908
9	13	12.717539647477949
9	14	7.157706551353364
9	15	5.955619723809388
9	16	-1.007089794371435
9	17	11.588396035151511
9	18	12.247959695028285
9	19	1.354312196948424
9	20	7.569955291589196
9	21	14.995053534761148
9	22	5.230572425076609
9	23	16.876076286221043
9	24	14.3538978891305
9	25	15.937682527928011
9	26	7.830982983913632
9	27	9.199873720562923
9	28	2.2719940218443586
9	29	5.211425122683654
9	30	6.927767666486151
9	31	10.535815774364929
9	32	11.254972502208119
9	33	11.35172891942925
9	34	8.918704162928941
9	35	11.274737564079977
9	36	13.443340096189273
9	37	11.014897771639657
9	38	11.225907374170948
9	39	11.512264585601457
9	40	8.088643341937154
10	1	6.474988986310848
10	2	1.6449236193129764
10	3	19.258440579927637
10	4	16.114721576681426
10	5	13.337820714635289
10	6	12.33046474358408
10	7	12.362015291256363
10	8	13.314760396557197
10	9	12.316702753892724
10	10	13.799625641557238
10	11	3.7345614214592047
10	12	8.8357379067461
10	13	5.840346665021812
10	14	7.31560128819547
10	15	6.973163583458511
10	16	9.992910205628565
10	17	10.500676736905898
10	18	12.177784256431792
10	19	12.670101670632633
10	20	10.780481607378668
10	21	12.293299148796237
10	22	17.844607512795907
10	23	18.630462251133324
10	24	17.17845929263927
10	25	16.463998317401696
10	26	13.462561931282053
10	27	12.620926352141872
10	28	11.377257179739095
10	29	13.983354947245058
10	30	10.664609771749308
10	31	9.676166651557912
10	32	8.13216548466426
10	33	7.807869270306446
10	34	8.480107671700873
10	35	8.397544581623837
10	36	7.881936587417341
10	37	7.260511806727376
10	38	5.997837198732352
10	39	8.021036515426019
10	40	7.948292464744171
11	1	-0.6829057505312548
11	2	-8.723497433318602
11	3	-1.5310331042828906
11	4	9.851563681944583
11	5	-8.872705601154184
11	6	-2.1432194669422344
11	7	-1.6379847087436374
11	8	-5.948397498179645
11	9	2.7377553854716687
11	10	-4.147742779495392
11	11	11.313508789880256
11	12	12.309422117272415
11	13	11.050872980811285
11	14	1.157706551353364
11	15	12.28895305714272
11	16	14.992910205628565
11	17	11.921729368484847
11	18	14.91462636169495
11	19	7.354312196948424
11	20	15.569955291589196
11	21	14.661720201427816
11	22	18.897239091743277
11	23	19.20940961955438
11	24	19.02056455579717
11	25	16.93768252792801
11	26	-10.169017016086368
11	27	-9.800126279437077
11	28	-9.728005978155641
11	29	-9.121908210649678
11	30	-8.072232333513849
11	31	-7.797517558968403
11	32	-7.078360831125213
11	33	-6.981604413904082
11	34	-6.414629170404391
11	35	-6.3919291025866904
11	36	-6.223326570477395
11	37	-6.318435561693676
11	38	-6.107425959162384
11	39	-5.821068747731875
11	40	-5.578023324729514
12	1	4.745665678040176
12	2	6.490788280967113
12	3	6.897538324288536
12	4	-0.1484363180554169
12	5	10.698722970274385
12	6	9.785351961629196
12	7	7.147729576970647
12	8	11.1944596446775
12	9	7.952041099757384
12	10	13.137971506218893
12	11	8.742080218451687
12	12	8.237993545843842
12	13	7.979444409382712
12	14	7.871992265639079
12	15	9.646095914285574
12	16	10.77862449134285
12	17	12.136015082770562
12	18	12.34319779026638
12	19	10.497169339805566
12	20	13.284241005874911
12	21	12.376005915713531
12	22	11.611524806028992
12	23	8.852266762411523
12	24	13.520564555797169
12	25	10.580539670785154
12	26	7.473840126770774
12	27	5.342730863420066
12	28	6.557708307558643
12	29	8.520948932207464
12	30	9.642053380771866
12	31	5.77391101246017
12	32	5.064496311731929
12	33	2.304109871810205
12	34	2.2282279724527516
12	35	2.5366423259847384
12	36	-1.151897999048824
12	37	2.7529930097348956
12	38	-0.46456881630524194
12	39	4.250359823696696
12	40	-2.7208804675866576
13	1	-5.882905750531254
13	2	-1.9234974333186017
13	3	-3.931033104282891
13	4	-5.548436318055417
13	5	0.12729439884581595
13	6	-3.943219466942235
13	7	-6.437984708743638
13	8	-4.348397498179644
13	9	-3.4622446145283305
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111	21	-12.338279798572184
111	22	-12.102760908256723
111	23	-11.79059038044562
111	24	-11.979435444202831
111	25	-12.062317472071989
111	26	-10.169017016086368
111	27	-9.800126279437077
111	28	-9.728005978155641
111	29	-9.121908210649678
111	30	-8.072232333513849
111	31	-7.797517558968403
111	32	-7.078360831125213
111	33	-6.981604413904082
111	34	-6.414629170404391
111	35	-6.3919291025866904
111	36	-6.223326570477395
111	37	-6.318435561693676
111	38	-6.107425959162384
111	39	-5.821068747731875
111	40	-5.578023324729514
112	1	5.245665678040176
112	2	9.84793113810997
112	3	6.040395467145682
112	4	7.922992253373156
112	5	7.198722970274389
112	6	5.14249481877205
112	7	-1.5665561373150645
112	8	3.9087453589632126
112	9	1.5948982426145264
112	10	3.852257220504608
112	11	2.0992230755945407
112	12	-0.9048635970132999
112	13	0.5508729808112847
112	14	3.729135122781937
112	15	2.574667342857005
112	16	0.8500530627714227
112	17	-2.50684206008658
112	18	-0.871087924019335
112	19	-2.9314020887658625
112	20	1.284241005874911
112	21	-0.4811369414293267
112	22	-1.7456180511138655
112	23	-1.6477332375884775
112	24	1.5205645557971685
112	25	-2.705174614929131
112	26	3.0452686981993473
112	27	0.4141594348486368
112	28	-0.9422916924413549
112	29	1.3780917893503215
112	30	2.0706248093432933
112	31	2.5596252981744545
112	32	2.921639168874787
112	33	1.0898241575244896
112	34	-0.5574863132615331
112	35	2.6080708974133096
112	36	-0.2947551419059664
112	37	0.6101358668777532
112	38	-2.464568816305241
112	39	-4.106783033446161
112	40	-3.8637376104438
114	1	2.983760916135413
114	2	6.054280344459176
114	3	8.69118911793933
114	4	-5.259547429166528
114	5	5.460627732179148
114	6	0.30122497750220845
114	7	-5.08242915318808
114	8	4.496046946264798
114	9	-6.373355725639442
114	10	1.1855905538379403
114	11	-0.5753800990086333
114	12	-6.023911216060917
114	13	-6.171349241410937
114	14	-5.620071226424415
114	15	2.1778419460316094
114	16	5.992910205628565
114	17	-3.3004928537373743
114	18	3.6924041394727283
114	19	2.687645530281756
114	20	-1.6522669306330258
114	21	6.1061646458722585
114	22	2.786127980632166
114	23	6.0982985084432695
114	24	6.0205645557971685
114	25	2.4932380834835683
114	26	-2.835683682753034
114	27	0.42209594278514473
114	28	-1.9502282003778628
114	29	-1.1219082106496785
114	30	-0.2944545557360705
114	31	1.5358157743649308
114	32	-3.8561386089029908
114	33	-3.537159969459637
114	34	-3.4146291704043907
114	35	-2.947484658142246
114	36	-3.66777101492184
114	37	-3.4295466728047863
114	38	-2.9963148480512736
114	39	-2.5988465255096527
114	40	-2.2446899913961813
116	1	-0.34957241719792265
116	2	1.7209470111258405
116	3	4.468966895717109
116	4	-4.5928807624998615
116	5	-0.09492782337640548
116	6	0.5234471997244334
116	7	2.584237513478584
116	8	0.3849358351536871
116	9	-0.04002239230610982
116	10	3.7411461093934975
116	11	-5.686491210119744
116	12	0.42053322838352614
116	13	-1.2824603525220475
116	14	-6.0645156708688575
116	15	0.5111752793649416
116	16	-2.8959786832603243
116	17	-3.1893817426262636
116	18	-2.863151416082829
116	19	-7.534576691940465
116	20	-3.096711375077472
116	21	-1.3382797985721844
116	22	-1.5472053527011678
116	23	-4.679479269334509
116	24	-3.9794354442028315
116	25	-3.728984138738655
116	26	-3.9467947938641466
116	27	-6.577904057214854
116	28	-7.061339311488974
116	29	-3.899685988427457
116	30	-3.6277878890694044
116	31	-3.6864064478572924
116	32	-3.078360831125213
116	33	-2.537159969459637
116	34	-3.4146291704043907
116	35	-2.947484658142246
116	36	-2.1122154593662845
116	37	-0.9851022283603417
116	38	-3.7740926258290513
116	39	-0.5988465255096536
116	40	-1.2446899913961804
117	1	7.317094249468745
117	2	9.276502566681398
117	3	19.46896689571711
117	4	15.851563681944583
117	5	13.127294398845816
117	6	10.856780533057766
117	7	11.362015291256363
117	8	4.051602501820355
117	9	11.737755385471669
117	10	5.852257220504608
117	11	-13.686491210119744
117	12	-7.690577882727585
117	13	3.0508729808112847
117	14	2.157706551353364
117	15	4.28895305714272
117	16	2.992910205628565
117	17	9.921729368484847
117	18	-1.0853736383050503
117	19	15.354312196948424
117	20	13.569955291589196
117	21	-12.338279798572184
117	22	-12.102760908256723
117	23	-11.79059038044562
117	24	-11.979435444202831
117	25	-12.062317472071989
117	26	-10.169017016086368
117	27	-9.800126279437077
117	28	-9.728005978155641
117	29	-9.121908210649678
117	30	-8.072232333513849
117	31	-7.797517558968403
117	32	-7.078360831125213
117	33	-6.981604413904082
117	34	-6.414629170404391
117	35	-6.3919291025866904
117	36	-6.223326570477395
117	37	-6.318435561693676
117	38	-6.107425959162384
117	39	-5.821068747731875
117	40	-5.578023324729514
118	1	6.317094249468745
118	2	7.276502566681398
118	3	18.46896689571711
118	4	9.851563681944583
118	5	7.127294398845816
118	6	8.856780533057766
118	7	11.362015291256363
118	8	7.051602501820355
118	9	11.737755385471669
118	10	-15.147742779495392
118	11	-19.686491210119744
118	12	-18.690577882727585
118	13	-19.949127019188715
118	14	-19.842293448646636
118	15	-18.71104694285728
118	16	-16.007089794371435
118	17	-17.078270631515153
118	18	-16.08537363830505
118	19	-13.645687803051576
118	20	-15.430044708410804
118	21	-12.338279798572184
118	22	-12.102760908256723
118	23	-11.79059038044562
118	24	-11.979435444202831
118	25	-12.062317472071989
118	26	-10.169017016086368
118	27	-9.800126279437077
118	28	-9.728005978155641
118	29	-9.121908210649678
118	30	-8.072232333513849
118	31	-7.797517558968403
118	32	-7.078360831125213
118	33	-6.981604413904082
118	34	-6.414629170404391
118	35	-6.3919291025866904
118	36	-6.223326570477395
118	37	-6.318435561693676
118	38	-6.107425959162384
118	39	-5.821068747731875
118	40	-5.578023324729514
119	1	4.817094249468745
119	2	11.776502566681398
119	3	13.46896689571711
119	4	4.851563681944583
119	5	0.12729439884581595
119	6	-2.1432194669422344
119	7	-1.1379847087436374
119	8	0.551602501820355
119	9	-1.7622446145283313
119	10	9.352257220504608
119	11	0.813508789880256
119	12	0.3094221172724154
119	13	-1.9491270191887153
119	14	-2.342293448646636
119	15	1.2889530571427201
119	16	-7.507089794371435
119	17	-9.078270631515153
119	18	-6.58537363830505
119	19	-0.645687803051576
119	20	-6.930044708410804
119	21	-2.8382797985721844
119	22	-5.602760908256723
119	23	-5.29059038044562
119	24	-3.4794354442028315
119	25	0.4376825279280112
119	26	0.830982983913632
119	27	-7.300126279437077
119	28	-0.7280059781556414
119	29	3.8780917893503215
119	30	-8.072232333513849
119	31	-7.797517558968403
119	32	-7.078360831125213
119	33	-6.981604413904082
119	34	-6.414629170404391
119	35	-6.3919291025866904
119	36	-6.223326570477395
119	37	-6.318435561693676
119	38	-6.107425959162384
119	39	-5.821068747731875
119	40	-5.578023324729514
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	1287.0
5	148.0
6	62.0
7	78.0
8	102.0
9	142.0
10	175.0
11	284.0
12	306.0
13	335.0
14	355.0
15	363.0
16	401.0
17	417.0
18	402.0
19	510.0
20	467.0
21	438.0
22	475.0
23	482.0
24	494.0
25	442.0
26	347.0
27	332.0
28	234.0
29	177.0
30	113.0
31	74.0
32	40.0
33	23.0
34	9.0
35	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.41151744430434	0.2942412778478352	0.16813787305590583	0.12610340479192939
8	97.74159663865547	0.7457983193277311	0.6302521008403361	0.8823529411764706
9	95.41920571548644	2.36394200462282	0.9981088463963018	1.2187434334944316
10	37.19806763285024	36.27389203948751	12.864944339424492	13.663095988237764
11	25.209995800084002	35.762284754304915	22.112557748845024	16.915161696766067
12	41.131996219678676	16.139871889110573	19.542161083692115	23.18597080751864
13	25.766484670306593	18.311633767324654	24.181016379672407	31.74086518269635
14	23.82402351952961	29.24191516169677	25.3044939101218	21.629567408651827
15	32.108357832843346	23.908021839563208	22.66904661906762	21.31457370852583
16	28.118437631247374	22.574548509029817	27.67744645107098	21.629567408651827
17	31.667366652666946	20.569088618227635	25.692986140277196	22.070558588828224
18	26.742965140697184	22.921041579168417	24.9685006299874	25.367492650146993
19	23.18306298714978	15.809985253844532	20.433958289445968	40.57299346955972
20	25.661486770264595	27.078958420831583	21.50356992860143	25.755984880302396
21	21.25157496850063	24.254514909701804	30.01889962200756	24.475010499790002
22	22.77404451910962	19.099118017639647	25.913481730365394	32.21335573288534
23	23.393532129357414	23.79252414951701	25.892482150356994	26.921461570768585
24	20.579588408231835	21.766064678706424	31.45737085258295	26.19697606047879
25	25.10504201680672	21.31302521008403	27.86764705882353	25.71428571428571
26	21.043203371970495	21.801896733403584	31.875658587987353	25.279241306638568
27	24.401511969760605	18.112137757244856	30.291894162116755	27.194456110877784
28	25.404326822096195	21.970174333123293	29.101029195547156	23.524469649233353
29	27.04719857037738	20.561337117628508	29.64364553768527	22.74781877430884
30	20.31759385844989	21.264065622042274	34.157114312756335	24.2612262067515
31	19.520655944497005	27.257437191212023	33.97456112687901	19.247345737411965
32	19.739605207895842	21.388072238555228	35.13229735405292	23.74002519949601
33	16.04367912641747	23.225535489290213	36.61276774464511	24.118017639647206
34	13.786224275514488	25.545989080218394	39.174716505669885	21.49307013859723
35	14.249711225454165	24.782106479050718	39.78788196996745	21.18030032552767
36	12.538065735587525	27.806363540900975	37.06815079281739	22.587419930694107
37	12.588000420300515	24.72417778711779	41.60975097194494	21.078070820636754
38	15.64797311489183	23.923545473639994	37.56563747111951	22.862843940348665
39	14.615707685846283	24.9055018899622	37.1797564048719	23.299034019319613
40	12.536749265014699	27.887442251154976	40.32969340613188	19.246115077698445
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.0
9	0.5
10	0.0
11	4.5
12	9.0
13	9.0
14	28.0
15	47.0
16	50.0
17	53.0
18	53.0
19	68.5
20	84.0
21	107.5
22	131.0
23	131.0
24	142.0
25	153.0
26	173.0
27	193.0
28	193.0
29	203.5
30	214.0
31	246.5
32	279.0
33	279.0
34	331.0
35	383.0
36	430.5
37	478.0
38	478.0
39	540.5
40	603.0
41	624.0
42	645.0
43	645.0
44	673.0
45	701.0
46	659.5
47	618.0
48	618.0
49	642.5
50	667.0
51	688.0
52	709.0
53	709.0
54	733.5
55	758.0
56	729.5
57	701.0
58	701.0
59	680.0
60	659.0
61	612.0
62	565.0
63	565.0
64	463.0
65	361.0
66	292.0
67	223.0
68	223.0
69	183.5
70	144.0
71	114.0
72	84.0
73	84.0
74	61.5
75	39.0
76	26.5
77	14.0
78	14.0
79	9.0
80	4.0
81	3.5
82	3.0
83	3.0
84	2.0
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.08399832003359933
8	0.041999160016799666
9	0.0629987400251995
10	0.020999580008399833
11	0.0
12	0.010499790004199917
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.3149937001259975
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.041999160016799666
26	0.3569928601427972
27	0.0
28	0.020999580008399833
29	0.11549769004619909
30	0.15749685006299874
31	0.11549769004619909
32	0.0
33	0.0
34	0.0
35	0.010499790004199917
36	0.010499790004199917
37	0.07349853002939942
38	0.020999580008399833
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	9524.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.92566148677027
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.80810965162763	88.07223855522889
2	2.7755568246716162	5.102897942041159
3	0.7424328954882925	2.0474590508189836
4	0.2055968018275271	0.7559848803023939
5	0.125642490005711	0.5774884502309954
6	0.045688178183894916	0.251994960100798
7	0.09137635636778983	0.5879882402351954
8	0.045688178183894916	0.33599328013439733
9	0.022844089091947458	0.18899622007559846
>10	0.125642490005711	1.4594708105837884
>50	0.011422044545973729	0.6194876102477951
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT	59	0.6194876102477951	No Hit
GATCTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	19	0.19949601007979842	No Hit
GATCTTGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT	17	0.1784964300713986	No Hit
GATCTTGGGGCTTAATTCTCATTCTCTCTCTCTCTCTCTC	15	0.15749685006299874	No Hit
GATCTTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT	13	0.1364972700545989	No Hit
GATCTTGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT	12	0.125997480050399	No Hit
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT	12	0.125997480050399	No Hit
GATCTTGGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAA	11	0.11549769004619909	No Hit
GATCTTGGGCTCGTCTTAATCAGCAACATCTTTTGATACA	10	0.10499790004199916	No Hit
GATCTTGGGTACCTGGTTGATCCTGCCCGTAGCCTCTGCT	10	0.10499790004199916	No Hit
GATCTTGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10	0.10499790004199916	No Hit
GATCTTGGGGAGTGCAGTGCTGATCTTGGGACACGCAGGG	10	0.10499790004199916	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.020999580008399833	0.0	0.0	0.0	0.0
15	0.020999580008399833	0.0	0.0	0.0	0.0
16	0.020999580008399833	0.0	0.0	0.0	0.0
17	0.020999580008399833	0.0	0.0	0.0	0.0
18	0.041999160016799666	0.0	0.0	0.0	0.0
19	0.041999160016799666	0.0	0.0	0.0	0.0
20	0.05249895002099958	0.0	0.0	0.0	0.0
21	0.05249895002099958	0.0	0.0	0.0	0.0
22	0.05249895002099958	0.0	0.0	0.0	0.0
23	0.05249895002099958	0.0	0.0	0.0	0.0
24	0.05249895002099958	0.0	0.0	0.0	0.0
25	0.06299874002519949	0.0	0.0	0.0	0.0
26	0.06299874002519949	0.0	0.0	0.0	0.0
27	0.06299874002519949	0.0	0.0	0.0	0.0
28	0.06299874002519949	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTTGA	20	0.00254411	34.287235	2
CTTGGGT	125	0.0	34.287235	4
TTGGGTG	50	3.074092E-10	34.287235	5
TTGGGTA	55	2.1827873E-11	34.287235	5
TGGGGGC	20	0.00254411	34.287235	6
TGGGGCT	20	0.00254411	34.287235	6
TTGGGCA	40	6.2378604E-8	34.287235	5
TGGGGAG	55	2.1827873E-11	34.287235	6
TGGGGAC	40	6.2378604E-8	34.287235	6
TGGGTGG	25	1.789022E-4	34.287235	6
TGGGTGC	20	0.00254411	34.287235	6
TGGGCAG	20	0.00254411	34.287235	6
TGGGTAG	20	0.00254411	34.287235	6
GGGAAGC	45	4.3837645E-9	34.287235	7
TGGGATC	20	0.00254411	34.287235	6
TGGGAAT	25	1.789022E-4	34.287235	6
GGGGAGT	35	8.8644447E-7	34.287235	7
GGGGAGC	20	0.00254411	34.287235	7
GGGTGGT	25	1.789022E-4	34.287235	7
GGGTACC	30	1.2591205E-5	34.28723	7
>>END_MODULE