FastQCFastQC Report
Fri 17 Jun 2016
SRR1525860_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525860_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2100
Sequences flagged as poor quality0
Sequence length40
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT100.4761904761904762No Hit
GATCTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT50.2380952380952381No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG40.19047619047619047No Hit
GATCTTGGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTT40.19047619047619047No Hit
GATCTTGGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTT40.19047619047619047No Hit
GATCTTGGGGCGTCGACTTTTTTTTTTTTTTTTTTTTTTT40.19047619047619047No Hit
GATCTTGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTTT30.14285714285714285No Hit
GATCTTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT30.14285714285714285No Hit
GATCTTGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.14285714285714285No Hit
GATCTTGGGGACTTTTCTTTTTCCTTTTTCTTTTTCTTTT30.14285714285714285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGGG307.772347E-634.658545
TGGGCAG200.00199428534.658546
TGGGAGT200.00199428534.658546
TTGGGAG251.2579857E-434.6585355
CTTGGGA650.031.9924954
CTTGGGC504.485628E-931.1926824
TTGGGGT401.3390072E-630.3262215
TCTTGGG2400.028.160063
TTGGGCA250.005958939427.7268285
ATCTTGG2600.027.3269212
GATCTTG2600.027.3269211
CTTGGGG1100.025.206214
Produced by FastQC (version 0.11.5)