##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525860_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2100
Sequences flagged as poor quality	0
Sequence length	40
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.293333333333333	4.0	4.0	25.0	4.0	30.0
2	10.10047619047619	4.0	4.0	18.0	4.0	26.0
3	10.398571428571428	4.0	4.0	15.0	4.0	26.0
4	7.438095238095238	4.0	4.0	11.0	4.0	15.0
5	10.26047619047619	4.0	4.0	19.0	4.0	25.0
6	10.609047619047619	4.0	4.0	20.0	4.0	27.0
7	10.463333333333333	4.0	4.0	20.0	4.0	25.0
8	11.334761904761905	4.0	4.0	23.0	4.0	29.0
9	10.404285714285715	4.0	4.0	20.0	4.0	27.0
10	8.922380952380953	4.0	4.0	13.0	4.0	24.0
11	10.217619047619047	4.0	4.0	14.0	4.0	31.0
12	10.053809523809523	4.0	4.0	15.0	4.0	30.0
13	9.720952380952381	4.0	4.0	12.0	4.0	29.0
14	9.57	4.0	4.0	11.0	4.0	30.0
15	9.183333333333334	4.0	4.0	8.0	4.0	29.0
16	7.700952380952381	4.0	4.0	4.0	4.0	22.0
17	7.258095238095238	4.0	4.0	4.0	4.0	19.0
18	6.938571428571429	4.0	4.0	4.0	4.0	18.0
19	7.164761904761905	4.0	4.0	4.0	4.0	19.0
20	7.278095238095238	4.0	4.0	4.0	4.0	20.0
21	7.395238095238096	4.0	4.0	4.0	4.0	21.0
22	7.223333333333334	4.0	4.0	4.0	4.0	20.0
23	7.338571428571429	4.0	4.0	4.0	4.0	20.0
24	7.249047619047619	4.0	4.0	4.0	4.0	20.0
25	7.437142857142857	4.0	4.0	4.0	4.0	21.0
26	7.8566666666666665	4.0	4.0	4.0	4.0	25.0
27	8.012380952380953	4.0	4.0	4.0	4.0	27.0
28	7.8185714285714285	4.0	4.0	4.0	4.0	24.0
29	7.744761904761905	4.0	4.0	4.0	4.0	23.0
30	7.8185714285714285	4.0	4.0	4.0	4.0	25.0
31	8.08	4.0	4.0	4.0	4.0	30.0
32	7.9871428571428575	4.0	4.0	4.0	4.0	30.0
33	7.934285714285714	4.0	4.0	4.0	4.0	29.0
34	7.787142857142857	4.0	4.0	4.0	4.0	27.0
35	7.757142857142857	4.0	4.0	4.0	4.0	27.0
36	7.7352380952380955	4.0	4.0	4.0	4.0	30.0
37	7.620952380952381	4.0	4.0	4.0	4.0	29.0
38	7.623333333333333	4.0	4.0	4.0	4.0	30.0
39	7.663809523809523	4.0	4.0	4.0	4.0	31.0
40	7.6761904761904765	4.0	4.0	4.0	4.0	30.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-8.508943089430897
1	2	-5.199186991869919
1	3	-6.092682926829269
1	4	-3.447662601626017
1	5	-5.760670731707318
1	6	-6.379878048780489
1	7	-5.534857723577238
1	8	-5.886483739837399
1	9	-5.312804878048782
1	10	-4.346849593495936
1	11	-5.003861788617886
1	12	-5.1934959349593495
1	13	-4.497459349593495
1	14	-4.607520325203252
1	15	-4.025304878048781
1	16	-3.5981707317073175
1	17	-3.4524390243902427
1	18	-2.810365853658536
1	19	-3.157723577235772
1	20	-2.9955284552845525
1	21	-3.4579268292682936
1	22	-3.0235772357723567
1	23	-3.30569105691057
1	24	-3.072967479674795
1	25	-3.6640243902439034
1	26	-3.822967479674797
1	27	-4.042886178861787
1	28	-3.9455284552845535
1	29	-3.7678861788617892
1	30	-3.9347560975609754
1	31	-3.8554878048780497
1	32	-3.973780487804878
1	33	-3.810772357723577
1	34	-3.941260162601627
1	35	-3.6640243902439016
1	36	-4.164430894308943
1	37	-3.97418699186992
1	38	-4.09369918699187
1	39	-3.7128048780487815
1	40	-4.049593495934962
4	1	-8.508943089430897
4	2	-5.199186991869919
4	3	-6.092682926829269
4	4	-3.447662601626017
4	5	-5.760670731707318
4	6	-6.379878048780489
4	7	-5.534857723577238
4	8	-5.886483739837399
4	9	-5.312804878048782
4	10	-4.346849593495936
4	11	-5.003861788617886
4	12	-5.1934959349593495
4	13	-4.497459349593495
4	14	-4.607520325203252
4	15	-4.025304878048781
4	16	-3.5981707317073175
4	17	-3.4524390243902427
4	18	-2.810365853658536
4	19	-3.157723577235772
4	20	-2.9955284552845525
4	21	-3.4579268292682936
4	22	-3.0235772357723567
4	23	-3.30569105691057
4	24	-3.072967479674795
4	25	-3.6640243902439034
4	26	-3.822967479674797
4	27	-4.042886178861787
4	28	-3.9455284552845535
4	29	-3.7678861788617892
4	30	-3.9347560975609754
4	31	-3.8554878048780497
4	32	-3.973780487804878
4	33	-3.810772357723577
4	34	-3.941260162601627
4	35	-3.6640243902439016
4	36	-4.164430894308943
4	37	-3.97418699186992
4	38	-4.09369918699187
4	39	-3.7128048780487815
4	40	-4.049593495934962
9	1	1.4910569105691032
9	2	0.6341463414634152
9	3	3.2406504065040647
9	4	0.05233739837398321
9	5	3.572662601626016
9	6	1.6201219512195113
9	7	5.465142276422762
9	8	6.946849593495934
9	9	1.853861788617884
9	10	2.4864837398373982
9	11	1.9961382113821138
9	12	2.4731707317073166
9	13	2.5025406504065053
9	14	2.392479674796748
9	15	1.3080284552845534
9	16	-2.4315040650406505
9	17	-2.4524390243902427
9	18	-1.4770325203252028
9	19	-2.157723577235772
9	20	-1.8288617886178855
9	21	-1.2912601626016267
9	22	0.30975609756097633
9	23	-1.139024390243903
9	24	1.2603658536585387
9	25	1.5026422764227627
9	26	0.677032520325203
9	27	0.790447154471547
9	28	1.0544715447154465
9	29	1.2321138211382108
9	30	1.0652439024390246
9	31	0.8111788617886164
9	32	-1.807113821138211
9	33	1.355894308943089
9	34	0.725406504065039
9	35	1.5026422764227645
9	36	0.5022357723577233
9	37	-2.140853658536587
9	38	0.5729674796747961
9	39	0.9538617886178846
9	40	-0.7162601626016292
10	1	14.991056910569103
10	2	16.80081300813008
10	3	24.90731707317073
10	4	22.052337398373982
10	5	21.239329268292682
10	6	19.12012195121951
10	7	25.96514227642276
10	8	25.6135162601626
10	9	26.18719512195122
10	10	15.153150406504064
10	11	13.996138211382114
10	12	11.30650406504065
10	13	22.502540650406505
10	14	19.892479674796746
10	15	21.97469512195122
10	16	24.401829268292683
10	17	27.047560975609755
10	18	22.689634146341465
10	19	17.34227642276423
10	20	28.004471544715447
10	21	-3.4579268292682936
10	22	-3.0235772357723567
10	23	-3.30569105691057
10	24	-3.072967479674795
10	25	-3.6640243902439034
10	26	-3.822967479674797
10	27	-4.042886178861787
10	28	-3.9455284552845535
10	29	-3.7678861788617892
10	30	-3.9347560975609754
10	31	-3.8554878048780497
10	32	-3.973780487804878
10	33	-3.810772357723577
10	34	-3.941260162601627
10	35	-3.6640243902439016
10	36	-4.164430894308943
10	37	-3.97418699186992
10	38	-4.09369918699187
10	39	-3.7128048780487815
10	40	-4.049593495934962
12	1	8.491056910569103
12	2	2.3008130081300813
12	3	11.65731707317073
12	4	1.8023373983739832
12	5	8.239329268292682
12	6	11.120121951219511
12	7	10.965142276422762
12	8	12.113516260162601
12	9	5.687195121951218
12	10	8.403150406504064
12	11	5.246138211382114
12	12	15.05650406504065
12	13	11.002540650406505
12	14	8.892479674796748
12	15	14.97469512195122
12	16	-2.0981707317073175
12	17	3.5475609756097573
12	18	3.939634146341464
12	19	0.8422764227642281
12	20	3.7544715447154475
12	21	4.292073170731706
12	22	4.726422764227643
12	23	4.44430894308943
12	24	4.677032520325205
12	25	4.085975609756097
12	26	3.927032520325203
12	27	3.707113821138213
12	28	3.8044715447154465
12	29	-0.7678861788617892
12	30	2.8152439024390246
12	31	3.8945121951219503
12	32	3.776219512195122
12	33	3.939227642276423
12	34	3.808739837398373
12	35	4.085975609756098
12	36	3.5855691056910572
12	37	3.77581300813008
12	38	3.65630081300813
12	39	4.0371951219512185
12	40	3.700406504065038
13	1	-3.708943089430896
13	2	-3.9991869918699186
13	3	-1.2926829268292686
13	4	-2.0476626016260164
13	5	-0.36067073170731767
13	6	-1.579878048780488
13	7	-0.7348577235772371
13	8	-0.6864837398373993
13	9	0.08719512195121837
13	10	-3.546849593495936
13	11	-3.603861788617886
13	12	0.6065040650406512
13	13	0.302540650406506
13	14	0.7924796747967484
13	15	-4.025304878048781
13	16	-3.5981707317073175
13	17	-3.4524390243902427
13	18	-2.810365853658536
13	19	-3.157723577235772
13	20	-2.9955284552845525
13	21	-3.4579268292682936
13	22	-3.0235772357723567
13	23	-3.30569105691057
13	24	-3.072967479674795
13	25	-3.6640243902439034
13	26	-3.822967479674797
13	27	-4.042886178861787
13	28	-3.9455284552845535
13	29	-3.7678861788617892
13	30	-3.9347560975609754
13	31	-3.8554878048780497
13	32	-3.973780487804878
13	33	-3.810772357723577
13	34	-3.941260162601627
13	35	-3.6640243902439016
13	36	-4.164430894308943
13	37	-3.97418699186992
13	38	-4.09369918699187
13	39	-3.7128048780487815
13	40	-4.049593495934962
14	1	9.491056910569103
14	2	9.800813008130081
14	3	16.90731707317073
14	4	5.552337398373983
14	5	8.239329268292682
14	6	12.620121951219511
14	7	14.465142276422762
14	8	16.1135162601626
14	9	17.68719512195122
14	10	10.653150406504064
14	11	24.996138211382114
14	12	20.80650406504065
14	13	5.502540650406505
14	14	24.392479674796746
14	15	24.97469512195122
14	16	20.401829268292683
14	17	20.547560975609755
14	18	8.189634146341465
14	19	14.842276422764229
14	20	17.004471544715447
14	21	18.542073170731705
14	22	7.976422764227643
14	23	7.69430894308943
14	24	15.927032520325206
14	25	18.335975609756098
14	26	14.177032520325202
14	27	4.957113821138213
14	28	13.054471544715447
14	29	22.23211382113821
14	30	-3.9347560975609754
14	31	-3.8554878048780497
14	32	-3.973780487804878
14	33	-3.810772357723577
14	34	-3.941260162601627
14	35	-3.6640243902439016
14	36	-4.164430894308943
14	37	-3.97418699186992
14	38	-4.09369918699187
14	39	-3.7128048780487815
14	40	-4.049593495934962
15	1	14.824390243902435
15	2	11.134146341463413
15	3	16.90731707317073
15	4	5.552337398373983
15	5	12.572662601626014
15	6	15.28678861788618
15	7	9.798475609756094
15	8	16.78018292682927
15	9	8.02052845528455
15	10	5.31981707317073
15	11	15.329471544715446
15	12	9.80650406504065
15	13	10.502540650406505
15	14	15.72581300813008
15	15	11.308028455284552
15	16	8.735162601626016
15	17	13.547560975609757
15	18	3.189634146341464
15	19	1.842276422764228
15	20	2.0044715447154475
15	21	-0.12459349593496061
15	22	-1.3569105691056897
15	23	-0.9723577235772369
15	24	2.593699186991871
15	25	-0.9973577235772364
15	26	1.177032520325203
15	27	2.623780487804879
15	28	1.3878048780487804
15	29	3.2321138211382108
15	30	3.0652439024390246
15	31	5.14451219512195
15	32	4.692886178861788
15	33	5.189227642276423
15	34	5.058739837398373
15	35	-3.6640243902439016
15	36	-4.164430894308943
15	37	-3.97418699186992
15	38	-4.09369918699187
15	39	-3.7128048780487815
15	40	-4.049593495934962
16	1	-8.508943089430897
16	2	-5.199186991869919
16	3	-6.092682926829269
16	4	-3.447662601626017
16	5	-5.760670731707318
16	6	-6.379878048780489
16	7	-5.534857723577238
16	8	-5.886483739837399
16	9	-5.312804878048782
16	10	-4.346849593495936
16	11	-5.003861788617886
16	12	-5.1934959349593495
16	13	-4.497459349593495
16	14	-4.607520325203252
16	15	-4.025304878048781
16	16	-3.5981707317073175
16	17	-3.4524390243902427
16	18	-2.810365853658536
16	19	-3.157723577235772
16	20	-2.9955284552845525
16	21	-3.4579268292682936
16	22	-3.0235772357723567
16	23	-3.30569105691057
16	24	-3.072967479674795
16	25	-3.6640243902439034
16	26	-3.822967479674797
16	27	-4.042886178861787
16	28	-3.9455284552845535
16	29	-3.7678861788617892
16	30	-3.9347560975609754
16	31	-3.8554878048780497
16	32	-3.973780487804878
16	33	-3.810772357723577
16	34	-3.941260162601627
16	35	-3.6640243902439016
16	36	-4.164430894308943
16	37	-3.97418699186992
16	38	-4.09369918699187
16	39	-3.7128048780487815
16	40	-4.049593495934962
17	1	-8.508943089430897
17	2	-5.199186991869919
17	3	-6.092682926829269
17	4	-3.447662601626017
17	5	-5.760670731707318
17	6	-6.379878048780489
17	7	-5.534857723577238
17	8	-5.886483739837399
17	9	-5.312804878048782
17	10	-4.346849593495936
17	11	-5.003861788617886
17	12	-5.1934959349593495
17	13	-4.497459349593495
17	14	-4.607520325203252
17	15	-4.025304878048781
17	16	-3.5981707317073175
17	17	-3.4524390243902427
17	18	-2.810365853658536
17	19	-3.157723577235772
17	20	-2.9955284552845525
17	21	-3.4579268292682936
17	22	-3.0235772357723567
17	23	-3.30569105691057
17	24	-3.072967479674795
17	25	-3.6640243902439034
17	26	-3.822967479674797
17	27	-4.042886178861787
17	28	-3.9455284552845535
17	29	-3.7678861788617892
17	30	-3.9347560975609754
17	31	-3.8554878048780497
17	32	-3.973780487804878
17	33	-3.810772357723577
17	34	-3.941260162601627
17	35	-3.6640243902439016
17	36	-4.164430894308943
17	37	-3.97418699186992
17	38	-4.09369918699187
17	39	-3.7128048780487815
17	40	-4.049593495934962
18	1	14.241056910569103
18	2	10.550813008130081
18	3	-0.09268292682926926
18	4	3.302337398373983
18	5	5.989329268292682
18	6	9.120121951219511
18	7	2.715142276422762
18	8	5.363516260162601
18	9	4.937195121951218
18	10	1.1531504065040643
18	11	-0.2538617886178862
18	12	-2.6934959349593495
18	13	1.7525406504065053
18	14	1.642479674796748
18	15	0.9746951219512194
18	16	-0.0981707317073175
18	17	1.2975609756097573
18	18	2.689634146341464
18	19	4.842276422764228
18	20	4.2544715447154475
18	21	4.042073170731706
18	22	3.2264227642276433
18	23	4.19430894308943
18	24	-0.07296747967479522
18	25	3.3359756097560966
18	26	0.927032520325203
18	27	5.457113821138213
18	28	9.304471544715447
18	29	7.482113821138211
18	30	9.565243902439025
18	31	11.644512195121951
18	32	11.526219512195123
18	33	10.939227642276423
18	34	11.058739837398374
18	35	11.085975609756098
18	36	11.585569105691057
18	37	11.52581300813008
18	38	11.65630081300813
18	39	11.787195121951218
18	40	11.700406504065038
19	1	9.491056910569103
19	2	12.800813008130081
19	3	4.907317073170731
19	4	7.552337398373983
19	5	10.239329268292682
19	6	12.620121951219511
19	7	10.465142276422762
19	8	12.113516260162601
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110	36	2.5855691056910572
110	37	2.77581300813008
110	38	2.65630081300813
110	39	3.0371951219512185
110	40	2.700406504065038
112	1	14.991056910569103
112	2	4.300813008130081
112	3	13.90731707317073
112	4	12.052337398373982
112	5	4.739329268292682
112	6	16.12012195121951
112	7	2.465142276422762
112	8	3.6135162601626014
112	9	4.187195121951218
112	10	3.1531504065040643
112	11	10.496138211382114
112	12	10.30650406504065
112	13	11.002540650406505
112	14	7.892479674796748
112	15	11.47469512195122
112	16	-3.5981707317073175
112	17	-3.4524390243902427
112	18	-2.810365853658536
112	19	-3.157723577235772
112	20	-2.9955284552845525
112	21	-3.4579268292682936
112	22	-3.0235772357723567
112	23	-3.30569105691057
112	24	-3.072967479674795
112	25	-3.6640243902439034
112	26	-3.822967479674797
112	27	-4.042886178861787
112	28	-3.9455284552845535
112	29	-3.7678861788617892
112	30	-3.9347560975609754
112	31	-3.8554878048780497
112	32	-3.973780487804878
112	33	-3.810772357723577
112	34	-3.941260162601627
112	35	-3.6640243902439016
112	36	-4.164430894308943
112	37	-3.97418699186992
112	38	-4.09369918699187
112	39	-3.7128048780487815
112	40	-4.049593495934962
118	1	-8.508943089430897
118	2	-5.199186991869919
118	3	-6.092682926829269
118	4	-3.447662601626017
118	5	-5.760670731707318
118	6	-6.379878048780489
118	7	-5.534857723577238
118	8	-5.886483739837399
118	9	-5.312804878048782
118	10	-4.346849593495936
118	11	-5.003861788617886
118	12	-5.1934959349593495
118	13	-4.497459349593495
118	14	-4.607520325203252
118	15	-4.025304878048781
118	16	-3.5981707317073175
118	17	-3.4524390243902427
118	18	-2.810365853658536
118	19	-3.157723577235772
118	20	-2.9955284552845525
118	21	-3.4579268292682936
118	22	-3.0235772357723567
118	23	-3.30569105691057
118	24	-3.072967479674795
118	25	-3.6640243902439034
118	26	-3.822967479674797
118	27	-4.042886178861787
118	28	-3.9455284552845535
118	29	-3.7678861788617892
118	30	-3.9347560975609754
118	31	-3.8554878048780497
118	32	-3.973780487804878
118	33	-3.810772357723577
118	34	-3.941260162601627
118	35	-3.6640243902439016
118	36	-4.164430894308943
118	37	-3.97418699186992
118	38	-4.09369918699187
118	39	-3.7128048780487815
118	40	-4.049593495934962
120	1	-8.508943089430897
120	2	-5.199186991869919
120	3	-6.092682926829269
120	4	-3.447662601626017
120	5	-5.760670731707318
120	6	-6.379878048780489
120	7	-5.534857723577238
120	8	-5.886483739837399
120	9	-5.312804878048782
120	10	-4.346849593495936
120	11	-5.003861788617886
120	12	-5.1934959349593495
120	13	-4.497459349593495
120	14	-4.607520325203252
120	15	-4.025304878048781
120	16	-3.5981707317073175
120	17	-3.4524390243902427
120	18	-2.810365853658536
120	19	-3.157723577235772
120	20	-2.9955284552845525
120	21	-3.4579268292682936
120	22	-3.0235772357723567
120	23	-3.30569105691057
120	24	-3.072967479674795
120	25	-3.6640243902439034
120	26	-3.822967479674797
120	27	-4.042886178861787
120	28	-3.9455284552845535
120	29	-3.7678861788617892
120	30	-3.9347560975609754
120	31	-3.8554878048780497
120	32	-3.973780487804878
120	33	-3.810772357723577
120	34	-3.941260162601627
120	35	-3.6640243902439016
120	36	-4.164430894308943
120	37	-3.97418699186992
120	38	-4.09369918699187
120	39	-3.7128048780487815
120	40	-4.049593495934962
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	1318.0
5	40.0
6	19.0
7	25.0
8	30.0
9	42.0
10	74.0
11	81.0
12	39.0
13	22.0
14	20.0
15	19.0
16	17.0
17	12.0
18	14.0
19	25.0
20	36.0
21	55.0
22	46.0
23	62.0
24	39.0
25	23.0
26	10.0
27	15.0
28	7.0
29	2.0
30	3.0
31	2.0
32	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.1866028708134	0.19138755980861244	0.4784688995215311	0.14354066985645933
8	98.66666666666667	0.5238095238095238	0.4761904761904762	0.33333333333333337
9	95.52380952380952	2.2857142857142856	0.8571428571428572	1.3333333333333335
10	38.285714285714285	30.238095238095237	11.666666666666666	19.80952380952381
11	27.95238095238095	26.380952380952383	24.0	21.666666666666668
12	41.80952380952381	13.857142857142858	20.238095238095237	24.095238095238095
13	23.904761904761905	17.952380952380953	26.285714285714285	31.857142857142858
14	23.15388280133397	24.106717484516434	24.821343496903285	27.918056217246306
15	29.23809523809524	16.57142857142857	27.57142857142857	26.61904761904762
16	27.047619047619047	19.0	30.38095238095238	23.57142857142857
17	30.619047619047617	16.523809523809526	27.0	25.857142857142858
18	19.19047619047619	21.285714285714285	30.523809523809526	28.999999999999996
19	18.776875298614428	18.20353559483994	28.905876731963687	34.11371237458194
20	23.523809523809526	21.904761904761905	25.476190476190474	29.095238095238095
21	18.80952380952381	24.666666666666668	29.619047619047617	26.904761904761905
22	18.0	21.761904761904763	30.476190476190478	29.761904761904763
23	18.714285714285715	20.38095238095238	28.904761904761905	32.0
24	16.857142857142858	22.857142857142858	34.38095238095238	25.9047619047619
25	18.589132507149667	17.683508102955194	30.695900857959963	33.03145853193518
26	19.580752739399713	19.05669366364936	29.20438303954264	32.15817055740829
27	22.933588150979457	18.20353559483994	29.718107978977542	29.14476827520306
28	22.449952335557676	22.6882745471878	26.596758817921828	28.265014299332698
29	21.316165951359086	22.317596566523605	28.898426323319025	27.467811158798284
30	18.333333333333332	22.238095238095237	34.23809523809524	25.19047619047619
31	16.238095238095237	24.428571428571427	32.904761904761905	26.42857142857143
32	15.95238095238095	21.142857142857142	33.476190476190474	29.428571428571427
33	13.190476190476192	19.857142857142858	36.57142857142857	30.38095238095238
34	12.19047619047619	21.761904761904763	39.61904761904762	26.42857142857143
35	9.761904761904763	22.76190476190476	40.476190476190474	27.0
36	11.270296084049667	26.07449856733524	38.25214899713467	24.40305635148042
37	11.688931297709923	26.669847328244273	40.17175572519084	21.469465648854964
38	12.761904761904763	23.57142857142857	38.952380952380956	24.714285714285715
39	12.523809523809524	22.666666666666664	39.19047619047619	25.61904761904762
40	10.666666666666668	22.714285714285715	39.38095238095238	27.23809523809524
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	2.0
12	3.0
13	3.0
14	3.5
15	4.0
16	17.0
17	30.0
18	30.0
19	30.0
20	30.0
21	39.0
22	48.0
23	48.0
24	46.0
25	44.0
26	51.5
27	59.0
28	59.0
29	60.5
30	62.0
31	67.0
32	72.0
33	72.0
34	69.5
35	67.0
36	67.5
37	68.0
38	68.0
39	75.5
40	83.0
41	98.5
42	114.0
43	114.0
44	120.5
45	127.0
46	118.0
47	109.0
48	109.0
49	123.0
50	137.0
51	142.0
52	147.0
53	147.0
54	151.0
55	155.0
56	156.5
57	158.0
58	158.0
59	153.0
60	148.0
61	140.5
62	133.0
63	133.0
64	114.0
65	95.0
66	93.5
67	92.0
68	92.0
69	76.5
70	61.0
71	42.5
72	24.0
73	24.0
74	17.5
75	11.0
76	11.0
77	11.0
78	11.0
79	7.5
80	4.0
81	2.5
82	1.0
83	1.0
84	1.0
85	1.0
86	0.5
87	0.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.4761904761904762
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.047619047619047616
15	0.0
16	0.0
17	0.0
18	0.0
19	0.33333333333333337
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.09523809523809523
26	0.047619047619047616
27	0.33333333333333337
28	0.09523809523809523
29	0.14285714285714285
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.2857142857142857
37	0.19047619047619047
38	0.0
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	2100.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.23809523809524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.28599412340843	95.57142857142857
2	1.2242899118511263	2.380952380952381
3	0.1958863858961802	0.5714285714285714
4	0.1958863858961802	0.7619047619047619
5	0.04897159647404505	0.2380952380952381
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.04897159647404505	0.4761904761904762
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCTTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT	10	0.4761904761904762	No Hit
GATCTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	5	0.2380952380952381	No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	4	0.19047619047619047	No Hit
GATCTTGGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTT	4	0.19047619047619047	No Hit
GATCTTGGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTT	4	0.19047619047619047	No Hit
GATCTTGGGGCGTCGACTTTTTTTTTTTTTTTTTTTTTTT	4	0.19047619047619047	No Hit
GATCTTGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTTT	3	0.14285714285714285	No Hit
GATCTTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT	3	0.14285714285714285	No Hit
GATCTTGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.14285714285714285	No Hit
GATCTTGGGGACTTTTCTTTTTCCTTTTTCTTTTTCTTTT	3	0.14285714285714285	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGGGGG	30	7.772347E-6	34.65854	5
TGGGCAG	20	0.001994285	34.65854	6
TGGGAGT	20	0.001994285	34.65854	6
TTGGGAG	25	1.2579857E-4	34.658535	5
CTTGGGA	65	0.0	31.992495	4
CTTGGGC	50	4.485628E-9	31.192682	4
TTGGGGT	40	1.3390072E-6	30.326221	5
TCTTGGG	240	0.0	28.16006	3
TTGGGCA	25	0.0059589394	27.726828	5
ATCTTGG	260	0.0	27.326921	2
GATCTTG	260	0.0	27.326921	1
CTTGGGG	110	0.0	25.20621	4
>>END_MODULE