FastQCFastQC Report
Fri 17 Jun 2016
SRR1525859_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525859_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7881
Sequences flagged as poor quality0
Sequence length40
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT3754.75827940616673No Hit
GATCTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT1061.3450069788097956No Hit
GATCTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG961.218119527978683No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC320.40603984265956095No Hit
GATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.40603984265956095No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG300.3806623524933384No Hit
GATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.317218627077782No Hit
GATCTTGGGACTAATCCTAGCCCTAGCCCTACACAAATAT250.317218627077782No Hit
GATCTTGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT250.317218627077782No Hit
GATCTTGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT230.29184113691155944No Hit
GATCTTGGGATGCCACAACTAGATACATCAACATGATTTA230.29184113691155944No Hit
GATCTTGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT230.29184113691155944No Hit
GATCTTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT230.29184113691155944No Hit
GATCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG210.26646364674533685No Hit
GATCTTGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACT170.21570866641289177No Hit
GATCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG150.1903311762466692No Hit
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC120.15226494099733537No Hit
GATCTTGGGTCACCGATCCTCTTCTGAAGGAGATTGGGCG120.15226494099733537No Hit
GATCTTGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT110.13957619591422407No Hit
GATCTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCG100.1268874508311128No Hit
GATCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.1268874508311128No Hit
GATCTTGGGTACCTGGTTGATACTGCCAGTAGCATATGCT100.1268874508311128No Hit
GATCTTGGGTACCTGGTTAATCCTGCCAGTAGCATATGCT100.1268874508311128No Hit
GATCTTGGGTACCTGGTTGACCCTGCCAGTAGCATATGCT100.1268874508311128No Hit
GATCTTGGGCGGGGAGGTAGTGACGAAAAATAACAATACA90.11419870574800152No Hit
GATCTTGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG90.11419870574800152No Hit
GATCTTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA90.11419870574800152No Hit
GATCTTGGGATGCCATCTACCTTCTTCAACCTCACCATAG80.10150996066489024No Hit
GATCTTGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT80.10150996066489024No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT80.10150996066489024No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGC80.10150996066489024No Hit
GATCTTGGGGAAACTGCGAATGGCTCATTAAATCAGTTAT80.10150996066489024No Hit
GATCTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA80.10150996066489024No Hit
GATCTTGGGTCATAGATCCTCTTCTGAGATGAGTTTTTGT80.10150996066489024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGGGG3900.033.9554184
TGGGGTT200.00264137733.9554186
GTTGATC601.8189894E-1233.95541416
TGGTTGA601.8189894E-1233.95541414
GTACCTG601.8189894E-1233.9554149
GGGTACC601.8189894E-1233.9554147
CTGGTTG552.1827873E-1133.95541413
ACCTGGT601.8189894E-1233.95541411
CTTGGGC359.2940354E-733.9554144
TTGGGGT552.1827873E-1133.9554145
TTGGGGG1650.033.9554145
TTGGGGC301.3172417E-533.9554145
TGGGGGT251.8654889E-433.9554146
TGGGGGG750.033.9554146
TGGGGGC301.3172417E-533.9554146
TGGGGGA359.2940354E-733.9554146
TTGGGAG359.2940354E-733.9554145
GGGGGGG251.8654889E-433.9554147
TGGGTAC601.8189894E-1233.9554146
TACCTGG601.8189894E-1233.95541410
Produced by FastQC (version 0.11.5)