##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525859_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7881
Sequences flagged as poor quality	0
Sequence length	40
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.987945692171046	30.0	4.0	33.0	4.0	35.0
2	12.713615023474178	9.0	4.0	20.0	4.0	30.0
3	14.575688364420758	12.0	4.0	26.0	4.0	33.0
4	14.83694962568202	7.0	4.0	26.0	4.0	33.0
5	14.46631138180434	4.0	4.0	27.0	4.0	33.0
6	13.529501332318233	4.0	4.0	25.0	4.0	31.0
7	13.657276995305164	4.0	4.0	27.0	4.0	32.0
8	13.469991117878442	4.0	4.0	26.0	4.0	33.0
9	12.728841517573912	4.0	4.0	25.0	4.0	32.0
10	11.765511990864104	4.0	4.0	22.0	4.0	31.0
11	11.106331683796473	4.0	4.0	19.0	4.0	32.0
12	10.908133485598274	4.0	4.0	19.0	4.0	31.0
13	10.097703337139956	4.0	4.0	15.0	4.0	29.0
14	10.163938586473797	4.0	4.0	13.0	4.0	30.0
15	10.277629742418474	4.0	4.0	13.0	4.0	31.0
16	8.329907372160893	4.0	4.0	4.0	4.0	26.0
17	8.160512625301358	4.0	4.0	4.0	4.0	25.0
18	7.75371145793681	4.0	4.0	4.0	4.0	23.0
19	7.635579241213044	4.0	4.0	4.0	4.0	22.0
20	7.642304276107093	4.0	4.0	4.0	4.0	22.0
21	7.565664255805101	4.0	4.0	4.0	4.0	23.0
22	7.402233219134628	4.0	4.0	4.0	4.0	22.0
23	7.508691790381931	4.0	4.0	4.0	4.0	23.0
24	7.276107093008502	4.0	4.0	4.0	4.0	21.0
25	7.452099987311255	4.0	4.0	4.0	4.0	23.0
26	7.448547138687983	4.0	4.0	4.0	4.0	23.0
27	7.408323816774521	4.0	4.0	4.0	4.0	22.0
28	7.4990483441187665	4.0	4.0	4.0	4.0	23.0
29	7.054307828955716	4.0	4.0	4.0	4.0	19.0
30	7.3409465803832	4.0	4.0	4.0	4.0	22.0
31	7.660068519223449	4.0	4.0	4.0	4.0	26.0
32	7.595102144397919	4.0	4.0	4.0	4.0	26.0
33	7.548661337393732	4.0	4.0	4.0	4.0	25.0
34	7.418728587742672	4.0	4.0	4.0	4.0	24.0
35	7.379901027788351	4.0	4.0	4.0	4.0	25.0
36	7.275726430656008	4.0	4.0	4.0	4.0	24.0
37	7.088313665778455	4.0	4.0	4.0	4.0	22.0
38	7.01484583174724	4.0	4.0	4.0	4.0	22.0
39	6.918665144017257	4.0	4.0	4.0	4.0	20.0
40	6.931227001649537	4.0	4.0	4.0	4.0	21.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	10.380962822070359
1	2	14.069396667466519
1	3	1.4821536850948593
1	4	-0.5163961234503471
1	5	10.41448511659909
1	6	3.0172853902633268
1	7	-3.435023599086101
1	8	-10.11933737048627
1	9	-9.575456094320986
1	10	-8.054812568966979
1	11	-7.017124296421171
1	12	-6.268536005967073
1	13	-5.408686369968537
1	14	-5.626235427154542
1	15	-6.130198348764525
1	16	-4.425851000000817
1	17	-4.04484108790175
1	18	-3.8514284411802784
1	19	-3.527706834967863
1	20	-3.540590244450538
1	21	-3.7961095787382595
1	22	-3.3890837495157333
1	23	-3.306075077904123
1	24	-3.3127473663319265
1	25	-3.5068216599236806
1	26	-3.6260367177416812
1	27	-3.3999103667302215
1	28	-3.5997143923614523
1	29	-3.2919244817682314
1	30	-3.65100724098886
1	31	-3.6907832822814433
1	32	-3.627058974692246
1	33	-3.757478523654995
1	34	-3.7064934546322377
1	35	-3.72320771378676
1	36	-3.375965039522023
1	37	-3.457390427519104
1	38	-3.1775354394380138
1	39	-3.102419647686191
1	40	-3.1134409673931707
3	1	10.130962822070359
3	2	11.819396667466519
3	3	1.9821536850948593
3	4	16.98360387654965
3	5	18.91448511659909
3	6	18.267285390263325
3	7	19.8149764009139
3	8	17.88066262951373
3	9	14.424543905679014
3	10	20.94518743103302
3	11	2.982875703578829
3	12	-0.26853600596707317
3	13	0.591313630031463
3	14	12.373764572845458
3	15	13.869801651235475
3	16	9.574148999999183
3	17	7.95515891209825
3	18	12.148571558819722
3	19	1.472293165032137
3	20	5.459409755549462
3	21	9.20389042126174
3	22	2.6109162504842667
3	23	3.693924922095877
3	24	2.6872526336680735
3	25	20.49317834007632
3	26	27.37396328225832
3	27	9.600089633269778
3	28	27.400285607638548
3	29	30.70807551823177
3	30	27.34899275901114
3	31	24.309216717718556
3	32	24.372941025307753
3	33	16.242521476345004
3	34	18.293506545367762
3	35	24.27679228621324
3	36	-3.375965039522023
3	37	-3.457390427519104
3	38	-3.1775354394380138
3	39	-3.102419647686191
3	40	-3.1134409673931707
4	1	8.130962822070359
4	2	-9.180603332533481
4	3	-11.01784631490514
4	4	-11.016396123450347
4	5	-11.08551488340091
4	6	-10.732714609736673
4	7	-10.1850235990861
4	8	-10.11933737048627
4	9	-9.575456094320986
4	10	-8.054812568966979
4	11	-7.017124296421171
4	12	-6.268536005967073
4	13	-5.408686369968537
4	14	-5.626235427154542
4	15	-6.130198348764525
4	16	-4.425851000000817
4	17	-4.04484108790175
4	18	-3.8514284411802784
4	19	-3.527706834967863
4	20	-3.540590244450538
4	21	-3.7961095787382595
4	22	-3.3890837495157333
4	23	-3.306075077904123
4	24	-3.3127473663319265
4	25	-3.5068216599236806
4	26	-3.6260367177416812
4	27	-3.3999103667302215
4	28	-3.5997143923614523
4	29	-3.2919244817682314
4	30	-3.65100724098886
4	31	-3.6907832822814433
4	32	-3.627058974692246
4	33	-3.757478523654995
4	34	-3.7064934546322377
4	35	-3.72320771378676
4	36	-3.375965039522023
4	37	-3.457390427519104
4	38	-3.1775354394380138
4	39	-3.102419647686191
4	40	-3.1134409673931707
5	1	5.464296155403691
5	2	3.374952223022076
5	3	5.648820351761527
5	4	14.872492765438542
5	5	11.358929561043533
5	6	3.156174279152216
5	7	0.25942084535834375
5	8	0.32510707395817384
5	9	1.3134327945679036
5	10	5.167409653255243
5	11	-1.5726798519767264
5	12	-0.4907582281892946
5	13	-0.1864641477463156
5	14	0.7070979061787916
5	15	-4.9079761265423025
5	16	-3.8702954444452615
5	17	-2.378174421235083
5	18	-1.1847617745136114
5	19	-1.19437350163453
5	20	-1.207256911117205
5	21	-0.7961095787382595
5	22	-0.9446393050712887
5	23	-0.306075077904123
5	24	-3.3127473663319265
5	25	-3.5068216599236806
5	26	-3.6260367177416812
5	27	-3.3999103667302215
5	28	-3.5997143923614523
5	29	-3.2919244817682314
5	30	-3.65100724098886
5	31	-3.6907832822814433
5	32	-3.627058974692246
5	33	-3.757478523654995
5	34	-3.7064934546322377
5	35	-3.72320771378676
5	36	-3.375965039522023
5	37	-3.457390427519104
5	38	-3.1775354394380138
5	39	-3.102419647686191
5	40	-3.1134409673931707
6	1	10.85318504429258
6	2	17.65273000079985
6	3	13.426598129539302
6	4	14.205826098771874
6	5	16.970040672154646
6	6	1.822840945818884
6	7	11.870531956469456
6	8	17.158440407291508
6	9	16.813432794567902
6	10	4.334076319921911
6	11	3.649542370245495
6	12	8.287019549588484
6	13	6.313535852253684
6	14	5.595986795067679
6	15	10.480912762346586
6	16	10.463037888888072
6	17	10.288492245431582
6	18	9.537460447708611
6	19	6.083404276143248
6	20	10.62607642221613
6	21	6.815001532372851
6	22	6.610916250484267
6	23	8.360591588762542
6	24	7.0205859670014075
6	25	6.882067228965209
6	26	8.540629948924988
6	27	6.766756299936445
6	28	7.122507829860769
6	29	6.096964407120658
6	30	7.626770536788919
6	31	9.531438939940777
6	32	10.150718803085532
6	33	11.464743698567226
6	34	9.737950989812205
6	35	9.88790339732435
6	36	8.401812738255757
6	37	7.87594290581423
6	38	9.378020116117543
6	39	7.3420247967582535
6	40	7.942114588162385
7	1	-0.6190371779296413
7	2	4.236063334133187
7	3	-2.9345129815718067
7	4	2.733603876549653
7	5	1.8311517832657582
7	6	-5.399381276403339
7	7	-2.518356932419435
7	8	4.047329296180397
7	9	2.841210572345682
7	10	6.361854097699689
7	11	3.482875703578829
7	12	11.231463994032927
7	13	10.091313630031463
7	14	4.290431239512124
7	15	8.036468317902143
7	16	8.740815666665851
7	17	11.70515891209825
7	18	10.481904892153054
7	19	10.888959831698806
7	20	7.626076422216128
7	21	10.787223754595072
7	22	7.944249583817601
7	23	7.610591588762543
7	24	6.2705859670014075
7	25	9.909845006742987
7	26	9.040629948924988
7	27	8.93342296660311
7	28	9.900285607638548
7	29	8.291408851565102
7	30	10.182326092344471
7	31	12.559216717718556
7	32	10.872941025307753
7	33	7.242521476345005
7	34	8.71017321203443
7	35	8.02679228621324
7	36	7.957368293811311
7	37	9.625942905814227
7	38	6.65579789389532
7	39	8.89758035231381
7	40	7.969892365940163
9	1	-2.3690371779296413
9	2	0.31939666746651874
9	3	1.9821536850948593
9	4	5.483603876549653
9	5	-3.0855148834009096
9	6	-2.7327146097366732
9	7	-2.185023599086101
9	8	-2.119337370486271
9	9	-1.5754560943209857
9	10	-2.0548125689669785
9	11	7.482875703578829
9	12	5.231463994032927
9	13	6.091313630031463
9	14	-2.6262354271545423
9	15	5.869801651235475
9	16	8.574148999999183
9	17	-4.04484108790175
9	18	-3.8514284411802784
9	19	-3.527706834967863
9	20	-3.540590244450538
9	21	-3.7961095787382595
9	22	-3.3890837495157333
9	23	-3.306075077904123
9	24	-3.3127473663319265
9	25	-3.5068216599236806
9	26	-3.6260367177416812
9	27	-3.3999103667302215
9	28	-3.5997143923614523
9	29	-3.2919244817682314
9	30	-3.65100724098886
9	31	-3.6907832822814433
9	32	-3.627058974692246
9	33	-3.757478523654995
9	34	-3.7064934546322377
9	35	-3.72320771378676
9	36	-3.375965039522023
9	37	-3.457390427519104
9	38	-3.1775354394380138
9	39	-3.102419647686191
9	40	-3.1134409673931707
10	1	-6.751390119106112
10	2	-3.651191567827599
10	3	-6.253140432552199
10	4	-3.5458078881562294
10	5	-6.379632530459734
10	6	-5.32094990385432
10	7	-1.1850235990861009
10	8	0.41007439421961145
10	9	-4.222514917850397
10	10	-0.1724596277905075
10	11	1.982875703578829
10	12	1.3196992881505736
10	13	1.6501371594432275
10	14	1.1972939846101642
10	15	3.105095768882533
10	16	3.16238429411683
10	17	1.8375118532747212
10	18	4.266218617643251
10	19	3.1781755179733127
10	20	4.518233284961227
10	21	4.4391845389087985
10	22	2.257975074013678
10	23	1.7527484515076415
10	24	3.099017339550427
10	25	3.0225901047822017
10	26	5.491610341081848
10	27	4.070677868563896
10	28	4.812050313520901
10	29	5.708075518231769
10	30	6.172522170775847
10	31	6.191569658895028
10	32	6.902352790013636
10	33	4.5366391234038295
10	34	6.175859486544233
10	35	6.335615815625005
10	36	4.271093784007388
10	37	4.777903690127954
10	38	3.5871704429149283
10	39	3.9564038817255733
10	40	2.827735503195065
11	1	5.318462822070359
11	2	-2.0556033325334813
11	3	5.107153685094859
11	4	6.046103876549653
11	5	4.66448511659909
11	6	-0.10771460973667324
11	7	-0.5600235990861009
11	8	-8.61933737048627
11	9	-8.137956094320986
11	10	-4.9298125689669785
11	11	-3.079624296421171
11	12	-3.456036005967073
11	13	-2.971186369968537
11	14	-2.2512354271545423
11	15	-1.3801983487645249
11	16	-3.113351000000817
11	17	-0.6698410879017498
11	18	-1.8514284411802784
11	19	-0.5277068349678631
11	20	-0.9155902444505379
11	21	-2.0461095787382595
11	22	-0.014083749515733324
11	23	0.693924922095877
11	24	0.6872526336680735
11	25	0.055678340076319444
11	26	0.8114632822583188
11	27	0.3500896332697785
11	28	0.4002856076385477
11	29	1.7080755182317686
11	30	1.2239927590111401
11	31	0.8092167177185567
11	32	1.560441025307754
11	33	0.6175214763450052
11	34	0.35600654536776233
11	35	-0.47320771378675985
11	36	0.12403496047797713
11	37	0.16760957248089614
11	38	-0.8025354394380138
11	39	-0.35241964768619116
11	40	0.6365590326068293
12	1	1.7976294887370265
12	2	2.9527300007998516
12	3	-4.351179648238475
12	4	3.0502705432163193
12	5	3.9144851165990904
12	6	0.7339520569299935
12	7	0.6816430675805663
12	8	-0.3193373704862701
12	9	-0.975456094320986
12	10	1.2785207643663554
12	11	3.2495423702454964
12	12	-0.8685360059670728
12	13	1.257980296698129
12	14	1.8404312395121245
12	15	0.6031349845688077
12	16	0.7741489999991824
12	17	1.2884922454315841
12	18	2.548571558819722
12	19	0.6722931650321362
12	20	0.9260764222161288
12	21	1.0038904212617412
12	22	1.6109162504842667
12	23	2.7605915887625434
12	24	1.0205859670014075
12	25	1.6265116734096523
12	26	3.5072966155916516
12	27	2.533422966603112
12	28	1.8669522743052145
12	29	1.0414088515651025
12	30	0.6823260923444741
12	31	2.5758833843852242
12	32	1.372941025307754
12	33	1.5758548096783391
12	34	0.02683987870109572
12	35	1.5434589528799076
12	36	2.5573682938113107
12	37	1.5426095724808961
12	38	0.8224645605619862
12	39	2.1642470189804763
12	40	1.0865590326068286
13	1	10.130962822070359
13	2	19.81939666746652
13	3	-3.0178463149051407
13	4	12.983603876549653
13	5	11.91448511659909
13	6	20.267285390263325
13	7	15.8149764009139
13	8	18.88066262951373
13	9	16.424543905679016
13	10	22.94518743103302
13	11	0.9828757035788289
13	12	13.731463994032927
13	13	4.591313630031463
13	14	16.37376457284546
13	15	18.869801651235477
13	16	23.57414899999918
13	17	24.95515891209825
13	18	18.14857155881972
13	19	4.472293165032137
13	20	6.459409755549462
13	21	22.20389042126174
13	22	27.610916250484266
13	23	18.693924922095878
13	24	6.6872526336680735
13	25	9.49317834007632
13	26	3.3739632822583188
13	27	2.6000896332697785
13	28	25.400285607638548
13	29	12.70807551823177
13	30	20.34899275901114
13	31	19.309216717718556
13	32	20.372941025307753
13	33	25.242521476345004
13	34	20.293506545367762
13	35	25.27679228621324
13	36	22.624034960477978
13	37	28.542609572480895
13	38	11.822464560561986
13	39	28.89758035231381
13	40	-3.1134409673931707
14	1	13.130962822070359
14	2	5.819396667466519
14	3	19.982153685094858
14	4	19.98360387654965
14	5	17.91448511659909
14	6	-10.732714609736673
14	7	-10.1850235990861
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116	27	-3.3999103667302215
116	28	-3.5997143923614523
116	29	-3.2919244817682314
116	30	-3.65100724098886
116	31	-3.6907832822814433
116	32	-3.627058974692246
116	33	-3.757478523654995
116	34	-3.7064934546322377
116	35	-3.72320771378676
116	36	-3.375965039522023
116	37	-3.457390427519104
116	38	-3.1775354394380138
116	39	-3.102419647686191
116	40	-3.1134409673931707
117	1	9.630962822070359
117	2	6.486063334133187
117	3	7.3154870184281915
117	4	9.483603876549653
117	5	9.747818449932423
117	6	2.7672853902633268
117	7	3.6483097342472313
117	8	13.38066262951373
117	9	14.091210572345682
117	10	0.4451874310330215
117	11	4.982875703578829
117	12	8.398130660699595
117	13	6.091313630031463
117	14	5.873764572845458
117	15	6.869801651235475
117	16	0.907482333332517
117	17	4.95515891209825
117	18	-2.8514284411802784
117	19	0.638959831698803
117	20	-2.540590244450538
117	21	-0.46277624540492646
117	22	0.9442495838176006
117	23	0.52725825542921
117	24	0.5205859670014066
117	25	0.3265116734096525
117	26	1.5406299489249848
117	27	1.9334229666031124
117	28	-0.0997143923614523
117	29	1.8747421848984347
117	30	0.8489927590111401
117	31	1.4758833843852228
117	32	0.5396076919744202
117	33	1.4091881430116713
117	34	1.4601732120344284
117	35	1.610125619546574
117	36	0.12403496047797713
117	37	1.7092762391475622
117	38	2.15579789389532
117	39	2.064247018980475
117	40	2.0532256992734954
118	1	-3.8690371779296413
118	2	-6.291714443644592
118	3	-11.01784631490514
118	4	-11.016396123450347
118	5	-11.08551488340091
118	6	-10.732714609736673
118	7	-10.1850235990861
118	8	-10.11933737048627
118	9	-9.575456094320986
118	10	-8.054812568966979
118	11	-7.017124296421171
118	12	-6.268536005967073
118	13	-5.408686369968537
118	14	-5.626235427154542
118	15	-6.130198348764525
118	16	-4.425851000000817
118	17	-4.04484108790175
118	18	-3.8514284411802784
118	19	-3.527706834967863
118	20	-3.540590244450538
118	21	-3.7961095787382595
118	22	-3.3890837495157333
118	23	-3.306075077904123
118	24	-3.3127473663319265
118	25	-3.5068216599236806
118	26	-3.6260367177416812
118	27	-3.3999103667302215
118	28	-3.5997143923614523
118	29	-3.2919244817682314
118	30	-3.65100724098886
118	31	-3.6907832822814433
118	32	-3.627058974692246
118	33	-3.757478523654995
118	34	-3.7064934546322377
118	35	-3.72320771378676
118	36	-3.375965039522023
118	37	-3.457390427519104
118	38	-3.1775354394380138
118	39	-3.102419647686191
118	40	-3.1134409673931707
119	1	-2.3690371779296413
119	2	-4.680603332533481
119	3	4.982153685094859
119	4	-2.391396123450347
119	5	1.4144851165990904
119	6	-0.48271460973667324
119	7	-3.810023599086101
119	8	2.630662629513729
119	9	-2.8254560943209857
119	10	-3.4298125689669785
119	11	1.857875703578829
119	12	3.856463994032927
119	13	1.466313630031463
119	14	-0.3762354271545423
119	15	0.7448016512354751
119	16	-4.425851000000817
119	17	-4.04484108790175
119	18	-3.8514284411802784
119	19	-3.527706834967863
119	20	-3.540590244450538
119	21	-3.7961095787382595
119	22	-3.3890837495157333
119	23	-3.306075077904123
119	24	-3.3127473663319265
119	25	-3.5068216599236806
119	26	-3.6260367177416812
119	27	-3.3999103667302215
119	28	-3.5997143923614523
119	29	-3.2919244817682314
119	30	-3.65100724098886
119	31	-3.6907832822814433
119	32	-3.627058974692246
119	33	-3.757478523654995
119	34	-3.7064934546322377
119	35	-3.72320771378676
119	36	-3.375965039522023
119	37	-3.457390427519104
119	38	-3.1775354394380138
119	39	-3.102419647686191
119	40	-3.1134409673931707
120	1	-6.035703844596309
120	2	-7.013936665866814
120	3	-4.351179648238475
120	4	-5.683062790117013
120	5	-3.5855148834009096
120	6	-4.899381276403339
120	7	-8.018356932419433
120	8	-7.452670703819604
120	9	-1.2421227609876517
120	10	-1.7214792356336446
120	11	2.316209036912163
120	12	-1.9352026726337392
120	13	-1.74201970330187
120	14	-0.9595687604878762
120	15	-6.130198348764525
120	16	-4.425851000000817
120	17	-4.04484108790175
120	18	-3.8514284411802784
120	19	-3.527706834967863
120	20	-3.540590244450538
120	21	-3.7961095787382595
120	22	-3.3890837495157333
120	23	-3.306075077904123
120	24	-3.3127473663319265
120	25	-3.5068216599236806
120	26	-3.6260367177416812
120	27	-3.3999103667302215
120	28	-3.5997143923614523
120	29	-3.2919244817682314
120	30	-3.65100724098886
120	31	-3.6907832822814433
120	32	-3.627058974692246
120	33	-3.757478523654995
120	34	-3.7064934546322377
120	35	-3.72320771378676
120	36	-3.375965039522023
120	37	-3.457390427519104
120	38	-3.1775354394380138
120	39	-3.102419647686191
120	40	-3.1134409673931707
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	3639.0
5	305.0
6	272.0
7	344.0
8	268.0
9	351.0
10	335.0
11	294.0
12	227.0
13	169.0
14	117.0
15	86.0
16	73.0
17	72.0
18	72.0
19	71.0
20	134.0
21	160.0
22	173.0
23	194.0
24	142.0
25	144.0
26	95.0
27	74.0
28	35.0
29	19.0
30	14.0
31	1.0
32	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.39039878079757	0.2159004318008636	0.25400050800101603	0.13970027940055882
8	98.18228041184696	0.851658828015762	0.49574170585992117	0.4703190542773611
9	96.87738004569688	0.8758568164508759	1.5105356689515106	0.7362274689007363
10	43.846056141242215	24.056903340530926	21.122824844404928	10.974215673821924
11	28.64576722934383	32.986419596395486	18.746033760629523	19.62177941363117
12	28.701941377997713	26.049993655627457	20.060905976398935	25.187158989975895
13	20.454834201499175	21.992122983102526	22.90687333248634	34.64616948291195
14	18.95151053566895	32.584412287382584	31.34044173648134	17.123635440467126
15	37.90128156325339	23.854840756249207	22.636721228270524	15.607156452226874
16	33.637863215328004	30.275345768303513	22.29412511102652	13.792665905341961
17	25.009516558812333	23.220403502093642	37.48255297551072	14.287526963583302
18	22.576142131979697	23.388324873096447	32.34771573604061	21.68781725888325
19	29.063413394332187	26.115135341212355	22.861863006735287	21.959588257720167
20	21.98959522903185	34.72909529247558	28.257835300088825	15.023474178403756
21	19.16000507549803	24.222814363659435	33.13031341200355	23.48686714883898
22	18.817408958254028	23.030072325846973	31.30313412003553	26.84938459586347
23	18.075653719218078	26.224930185326222	29.3983244478294	26.3010916476263
24	18.157594213932242	26.088059890876792	32.71158482426088	23.042761070930084
25	29.04495808991618	25.71755143510287	27.266954533909065	17.970535941071883
26	26.982714794102698	25.533807829181494	21.568378240976106	25.915099135739705
27	18.62033855161003	31.296932671503118	21.48402698230877	28.598701794578083
28	22.82884713052311	38.115794819705435	19.908583037074656	19.1467750126968
29	33.86378103119033	29.560789306174414	19.592616168045833	16.982813494589433
30	24.606598984771573	26.30710659898477	32.931472081218274	16.154822335025383
31	24.01015228426396	37.15736040609137	22.18274111675127	16.6497461928934
32	33.324853726787076	28.211650979394555	21.394047316204528	17.069447977613837
33	25.37749016622256	29.640908514147952	21.10138307321406	23.88021824641543
34	19.80713107473671	33.48559827433067	30.757518081461743	15.94975256947088
35	17.485090724527343	29.120669965740387	38.2438776804974	15.150361629234869
36	21.675565725908974	37.12178998220188	24.25629290617849	16.946351385710653
37	21.408629441624367	28.51522842639594	34.23857868020305	15.83756345177665
38	30.54568527918782	27.436548223350254	25.291878172588834	16.725888324873097
39	21.967005076142133	28.66751269035533	21.63705583756345	27.72842639593909
40	21.16751269035533	26.129441624365484	35.32994923857868	17.373096446700508
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.5
7	3.0
8	3.0
9	3.0
10	3.0
11	29.0
12	55.0
13	55.0
14	71.5
15	88.0
16	79.0
17	70.0
18	70.0
19	66.0
20	62.0
21	57.5
22	53.0
23	53.0
24	59.0
25	65.0
26	76.0
27	87.0
28	87.0
29	105.0
30	123.0
31	140.5
32	158.0
33	158.0
34	201.0
35	244.0
36	297.5
37	351.0
38	351.0
39	432.5
40	514.0
41	567.0
42	620.0
43	620.0
44	619.5
45	619.0
46	683.5
47	748.0
48	748.0
49	904.0
50	1060.0
51	940.0
52	820.0
53	820.0
54	726.0
55	632.0
56	610.0
57	588.0
58	588.0
59	517.5
60	447.0
61	353.0
62	259.0
63	259.0
64	187.5
65	116.0
66	85.0
67	54.0
68	54.0
69	41.0
70	28.0
71	18.5
72	9.0
73	9.0
74	6.0
75	3.0
76	2.5
77	2.0
78	2.0
79	1.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.08882121558177897
8	0.17764243116355793
9	0.03806623524933384
10	0.10150996066489024
11	0.02537749016622256
12	0.0
13	0.1268874508311128
14	0.03806623524933384
15	0.0
16	0.0
17	0.0
18	0.01268874508311128
19	0.15226494099733537
20	0.0
21	0.0
22	0.0
23	0.03806623524933384
24	0.0
25	0.08882121558177897
26	0.16495368608044664
27	0.30452988199467074
28	0.0634437254155564
29	0.3299073721608933
30	0.01268874508311128
31	0.01268874508311128
32	0.24108615657911434
33	0.0
34	0.0
35	0.0
36	0.1903311762466692
37	0.01268874508311128
38	0.01268874508311128
39	0.01268874508311128
40	0.01268874508311128
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	7881.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.42913335871083
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.29898074745186	72.38929069914985
2	5.080084128781751	7.968531912193884
3	1.0030739362562693	2.360106585458698
4	0.6633230868791458	2.08095419363025
5	0.24267917812651674	0.9516558812333461
6	0.0970716712506067	0.4567948229920061
7	0.06471444750040446	0.35528486232711587
8	0.11325028312570783	0.7105697246542317
9	0.04853583562530335	0.34259611724400457
>10	0.33975084937712347	5.062809288161401
>50	0.016178611875101116	1.218119527978683
>100	0.03235722375020223	6.103286384976526
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT	375	4.75827940616673	No Hit
GATCTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT	106	1.3450069788097956	No Hit
GATCTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG	96	1.218119527978683	No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	32	0.40603984265956095	No Hit
GATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32	0.40603984265956095	No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	30	0.3806623524933384	No Hit
GATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25	0.317218627077782	No Hit
GATCTTGGGACTAATCCTAGCCCTAGCCCTACACAAATAT	25	0.317218627077782	No Hit
GATCTTGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT	25	0.317218627077782	No Hit
GATCTTGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT	23	0.29184113691155944	No Hit
GATCTTGGGATGCCACAACTAGATACATCAACATGATTTA	23	0.29184113691155944	No Hit
GATCTTGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT	23	0.29184113691155944	No Hit
GATCTTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT	23	0.29184113691155944	No Hit
GATCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG	21	0.26646364674533685	No Hit
GATCTTGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACT	17	0.21570866641289177	No Hit
GATCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG	15	0.1903311762466692	No Hit
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC	12	0.15226494099733537	No Hit
GATCTTGGGTCACCGATCCTCTTCTGAAGGAGATTGGGCG	12	0.15226494099733537	No Hit
GATCTTGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT	11	0.13957619591422407	No Hit
GATCTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCG	10	0.1268874508311128	No Hit
GATCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10	0.1268874508311128	No Hit
GATCTTGGGTACCTGGTTGATACTGCCAGTAGCATATGCT	10	0.1268874508311128	No Hit
GATCTTGGGTACCTGGTTAATCCTGCCAGTAGCATATGCT	10	0.1268874508311128	No Hit
GATCTTGGGTACCTGGTTGACCCTGCCAGTAGCATATGCT	10	0.1268874508311128	No Hit
GATCTTGGGCGGGGAGGTAGTGACGAAAAATAACAATACA	9	0.11419870574800152	No Hit
GATCTTGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG	9	0.11419870574800152	No Hit
GATCTTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA	9	0.11419870574800152	No Hit
GATCTTGGGATGCCATCTACCTTCTTCAACCTCACCATAG	8	0.10150996066489024	No Hit
GATCTTGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT	8	0.10150996066489024	No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT	8	0.10150996066489024	No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGC	8	0.10150996066489024	No Hit
GATCTTGGGGAAACTGCGAATGGCTCATTAAATCAGTTAT	8	0.10150996066489024	No Hit
GATCTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA	8	0.10150996066489024	No Hit
GATCTTGGGTCATAGATCCTCTTCTGAGATGAGTTTTTGT	8	0.10150996066489024	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.01268874508311128	0.0	0.0	0.0	0.0
15	0.01268874508311128	0.0	0.0	0.0	0.0
16	0.01268874508311128	0.0	0.0	0.0	0.0
17	0.01268874508311128	0.0	0.0	0.0	0.0
18	0.02537749016622256	0.0	0.0	0.0	0.0
19	0.02537749016622256	0.0	0.0	0.0	0.0
20	0.02537749016622256	0.0	0.0	0.0	0.0
21	0.03806623524933384	0.0	0.0	0.0	0.0
22	0.03806623524933384	0.0	0.0	0.0	0.0
23	0.03806623524933384	0.0	0.0	0.0	0.0
24	0.0634437254155564	0.0	0.0	0.0	0.0
25	0.0634437254155564	0.0	0.0	0.0	0.0
26	0.0634437254155564	0.0	0.0	0.0	0.0
27	0.07613247049866768	0.0	0.0	0.0	0.0
28	0.07613247049866768	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGGGG	390	0.0	33.955418	4
TGGGGTT	20	0.002641377	33.955418	6
GTTGATC	60	1.8189894E-12	33.955414	16
TGGTTGA	60	1.8189894E-12	33.955414	14
GTACCTG	60	1.8189894E-12	33.955414	9
GGGTACC	60	1.8189894E-12	33.955414	7
CTGGTTG	55	2.1827873E-11	33.955414	13
ACCTGGT	60	1.8189894E-12	33.955414	11
CTTGGGC	35	9.2940354E-7	33.955414	4
TTGGGGT	55	2.1827873E-11	33.955414	5
TTGGGGG	165	0.0	33.955414	5
TTGGGGC	30	1.3172417E-5	33.955414	5
TGGGGGT	25	1.8654889E-4	33.955414	6
TGGGGGG	75	0.0	33.955414	6
TGGGGGC	30	1.3172417E-5	33.955414	6
TGGGGGA	35	9.2940354E-7	33.955414	6
TTGGGAG	35	9.2940354E-7	33.955414	5
GGGGGGG	25	1.8654889E-4	33.955414	7
TGGGTAC	60	1.8189894E-12	33.955414	6
TACCTGG	60	1.8189894E-12	33.955414	10
>>END_MODULE