FastQCFastQC Report
Fri 17 Jun 2016
SRR1525858_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525858_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69669
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC12211.7525728803341516No Hit
TAGTCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT9251.3277067275258725No Hit
TAGTCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC3820.5483069945025765No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2420.34735678709325524No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2350.33730927672278915No Hit
TAGTCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT1780.2554938351347084No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1450.20812700053108268No Hit
TAGTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC1440.20669164190673042No Hit
TAGTCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC1180.16937231767357075No Hit
TAGTCGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGAGCTCGTATGCCGT1160.16650160042486617Illumina Single End Adapter 2 (95% over 24bp)
TAGTCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA1110.15932480730310467No Hit
TAGTCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC1080.1550187314300478No Hit
TAGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1020.14640657968393403No Hit
TAGTCGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT970.13922978656217255No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA870.1248762003186496No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG780.11195797269947895No Hit
TAGTCGGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC760.10908725545077438No Hit
TAGTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCA730.10478117957771749No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTCA150.00815438450.37309341
GATTGAC550.050.37309341
CGATTGA354.953654E-850.29992740
GCGCAGA150.00820141650.29992738
GCCGATT354.953654E-850.29992738
ATTTATC150.00822500850.26342446
ACTATTA150.00822500850.26342447
ACCGCTC150.00822500850.26342445
AGACCAC150.00822500850.26342437
AGTACAC150.00822500850.26342447
AAGAGAC150.00827234250.19058235
TAAGGGC150.00827234250.19058214
GTGCATT204.1084003E-450.11794742
TTCCGCC150.00831987950.11794744
CATTCCG150.00831987950.11794742
GCATTTA150.00831987950.11794744
TCATCCG150.00831987950.11794742
ATGCAGT150.00831987950.11794743
ATGATTT150.00841556249.97330529
CTAAATG150.00841556249.97330528
Produced by FastQC (version 0.11.5)