##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525858_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	69669
Sequences flagged as poor quality	0
Sequence length	56
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.8318046763984	38.0	37.0	39.0	33.0	40.0
2	34.1478132311358	38.0	35.0	39.0	17.0	40.0
3	35.583085733970634	38.0	35.0	39.0	28.0	40.0
4	35.66708292066773	38.0	35.0	39.0	27.0	40.0
5	36.04135268196759	38.0	36.0	39.0	29.0	40.0
6	36.01030587492285	38.0	35.0	39.0	30.0	40.0
7	36.34574918543398	38.0	36.0	39.0	31.0	40.0
8	36.1811566119795	38.0	36.0	39.0	31.0	40.0
9	36.140220184012975	38.0	36.0	39.0	31.0	40.0
10	35.18874965910233	38.0	35.0	39.0	27.0	40.0
11	34.67144641088576	38.0	34.0	39.0	26.0	40.0
12	33.67549412220643	38.0	32.0	39.0	21.0	40.0
13	33.125034089767325	37.0	31.0	39.0	19.0	40.0
14	33.86443037792993	38.0	33.0	39.0	22.0	40.0
15	32.42674647260618	36.0	30.0	39.0	17.0	40.0
16	32.28890898390963	36.0	30.0	39.0	18.0	40.0
17	31.209605419914165	35.0	27.0	38.0	15.0	40.0
18	30.785313410555627	35.0	26.0	38.0	14.0	40.0
19	31.998076619443367	36.0	29.0	38.0	17.0	40.0
20	32.613744994186796	36.0	30.0	39.0	20.0	40.0
21	33.06110321663868	38.0	31.0	39.0	19.0	40.0
22	32.342246910390564	37.0	30.0	39.0	17.0	40.0
23	33.023611649370594	38.0	31.0	39.0	19.0	40.0
24	32.33585956451219	37.0	30.0	39.0	17.0	40.0
25	32.829565516944406	37.0	31.0	39.0	19.0	40.0
26	33.03678824154215	38.0	32.0	39.0	18.0	40.0
27	33.34514633481175	38.0	33.0	39.0	20.0	40.0
28	33.43754036946131	38.0	33.0	39.0	21.0	40.0
29	32.84604343395197	37.0	31.0	39.0	18.0	40.0
30	32.711808695402546	37.0	31.0	39.0	17.0	40.0
31	33.259024817350614	38.0	33.0	39.0	19.0	40.0
32	33.239259929093286	38.0	33.0	39.0	19.0	40.0
33	32.99207682039358	37.0	33.0	39.0	18.0	40.0
34	32.86259311889076	37.0	32.0	39.0	18.0	40.0
35	32.89587908538948	37.0	33.0	39.0	17.0	40.0
36	32.085489959666425	36.0	31.0	38.0	11.0	40.0
37	31.878209820723708	36.0	31.0	38.0	10.0	40.0
38	31.16297061820896	36.0	30.0	38.0	4.0	40.0
39	29.978957642566996	35.0	27.0	38.0	4.0	40.0
40	27.933083580932696	34.0	19.0	38.0	4.0	39.0
41	25.850650935136144	31.0	14.0	37.0	4.0	39.0
42	26.568100589932396	31.0	18.0	37.0	4.0	39.0
43	26.960412809140365	32.0	19.0	38.0	4.0	39.0
44	26.00786576526145	31.0	15.0	37.0	4.0	39.0
45	26.755515365514075	32.0	19.0	37.0	4.0	39.0
46	24.84423488208529	30.0	13.0	36.0	4.0	39.0
47	23.925117340567542	28.0	9.0	35.0	4.0	39.0
48	24.494538460434338	29.0	11.0	36.0	4.0	39.0
49	23.59330548737602	28.0	6.0	35.0	4.0	39.0
50	24.272043520073492	29.0	4.0	36.0	4.0	39.0
51	24.562430923366204	30.0	4.0	36.0	4.0	39.0
52	24.35862435229442	30.0	4.0	36.0	4.0	39.0
53	24.21761472103805	30.0	4.0	36.0	4.0	39.0
54	23.794456644992753	30.0	4.0	36.0	4.0	39.0
55	23.67869497193874	30.0	4.0	36.0	4.0	39.0
56	22.534154358466463	28.0	4.0	36.0	4.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	2.8176025604941444
1	2	5.92859891964077
1	3	3.8656167839592044
1	4	3.7673234107345976
1	5	3.6850946419483037
1	6	3.7670223300253554
1	7	3.260699989213876
1	8	3.4327605899638485
1	9	3.4176216196094558
1	10	4.280808845192837
1	11	-0.7569383421919937
1	12	-7.696894466312848
1	13	-18.673008814844057
1	14	-6.6314527664673975
1	15	0.78364626022789
1	16	-1.7779530896525202
1	17	-5.565887106026182
1	18	-4.45477234884579
1	19	0.1026259436985093
1	20	-0.6878420664348575
1	21	6.143560175539399
1	22	6.984998640705779
1	23	6.38915484452151
1	24	6.591070932498905
1	25	5.974053434787148
1	26	6.4094336096510744
1	27	6.430946795627676
1	28	5.72173322553407
1	29	6.923284259533208
1	30	7.159699971054568
1	31	4.375563418094444
1	32	3.6988968990182904
1	33	4.858605794582008
1	34	4.102796294269368
1	35	4.855366214806281
1	36	4.405390732327955
1	37	4.364031788614252
1	38	4.322133875923527
1	39	3.7987597558935278
1	40	-9.178578148114486
1	41	0.7267540909849153
1	42	-0.8686134563169006
1	43	-8.987149224048524
1	44	-14.211293456210177
1	45	-2.324214355904779
1	46	-4.179129164593689
1	47	-8.240731584971709
1	48	-4.364601379602458
1	49	-12.86962447243553
1	50	-3.178815375490469
1	51	2.802341832431079
1	52	2.6400359822642407
1	53	2.9787960988818973
1	54	3.0681639476284843
1	55	3.0890439165798433
1	56	5.6725545970276805
2	1	3.3568472238767413
2	2	6.656349330149801
2	3	5.039262104156251
2	4	4.6887519821631685
2	5	4.4601356928514235
2	6	4.484427912948178
2	7	4.0978921074404795
2	8	4.303942855973695
2	9	4.236176627819638
2	10	5.236638073435863
2	11	4.163751312980416
2	12	0.5568822167741843
2	13	1.938074928998308
2	14	5.084310780330632
2	15	5.995797327551372
2	16	5.088385169789188
2	17	7.485508624679898
2	18	5.457132413058972
2	19	6.742773726949739
2	20	5.813882071496174
2	21	7.260062638593588
2	22	7.768003566814155
2	23	6.6586950744065625
2	24	7.884831195224681
2	25	7.071836686018674
2	26	6.297446745940071
2	27	6.112227583805016
2	28	6.581503340476605
2	29	6.935763734081643
2	30	6.963476654141598
2	31	5.65856834420282
2	32	6.0291924655207545
2	33	6.021003167324206
2	34	5.732599249934388
2	35	6.29723813598855
2	36	7.840201898173607
2	37	7.542356911766955
2	38	8.275910559010555
2	39	8.273472399571688
2	40	6.5625548568116265
2	41	10.597443746157328
2	42	9.164966182435155
2	43	8.439944372010586
2	44	-3.049142388886697
2	45	8.972419470039394
2	46	6.259704989757708
2	47	3.271173176933054
2	48	7.101162167195568
2	49	-0.49737488294456256
2	50	6.791956381488184
2	51	11.070404229803824
2	52	10.737737131689528
2	53	10.563443061115063
2	54	10.323746870452787
2	55	9.73707347323009
2	56	11.192423234137696
3	1	3.4508048322285276
3	2	6.783207919203896
3	3	4.600872354077161
3	4	4.782788679410878
3	5	4.283609277160188
3	6	4.5959432561939835
3	7	4.052574170078884
3	8	3.8791563959926805
3	9	4.120548661986042
3	10	4.816676036105903
3	11	4.391685511019013
3	12	4.979296009877629
3	13	5.379153701538645
3	14	5.321102934712158
3	15	0.5971892921195447
3	16	6.473073559539269
3	17	8.029482050810866
3	18	8.28511406443512
3	19	7.11599422679156
3	20	6.402066190445879
3	21	6.830847200441099
3	22	7.9302148923440505
3	23	6.5793689117997936
3	24	7.125103261026641
3	25	6.946006252611529
3	26	6.2835271002583255
3	27	5.172493322495299
3	28	5.779225580274133
3	29	5.647880152936441
3	30	6.223046410547791
3	31	4.852889761475836
3	32	4.791688511075964
3	33	5.090759573524775
3	34	4.5491921002982
3	35	4.6485510116608
3	36	6.430205061729442
3	37	6.21619430499695
3	38	6.712085820003907
3	39	7.825671070878236
3	40	9.024829453458256
3	41	12.39647886162712
3	42	11.314523284618009
3	43	11.524253135060256
3	44	10.939864254580563
3	45	11.151713997087793
3	46	12.914798314654888
3	47	13.089980516382596
3	48	12.84483505552205
3	49	13.42622524359767
3	50	12.19436068392412
3	51	12.52492811465039
3	52	12.123216410398797
3	53	12.24257941037163
3	54	12.387255253875757
3	55	13.05208454567552
3	56	12.505931617560666
4	1	3.1397557696038803
4	2	6.514520244692527
4	3	4.003504982716969
4	4	4.4952737212936
4	5	4.07722714712429
4	6	4.205117568120592
4	7	3.541445330828786
4	8	3.8426984781626032
4	9	3.774557437414842
4	10	4.641885449747704
4	11	4.895235570851483
4	12	5.54451340118198
4	13	6.333202365280169
4	14	5.616994438501543
4	15	6.272259096666815
4	16	7.316870926910628
4	17	8.644878939522474
4	18	8.828871543493754
4	19	7.608216005810309
4	20	7.312157933565139
4	21	7.154740299762999
4	22	7.589967584805162
4	23	7.305096873507015
4	24	7.447799710966809
4	25	6.729332937892739
4	26	6.219785576524778
4	27	5.722872261466186
4	28	5.510139022635524
4	29	6.131565833032173
4	30	5.557629577679823
4	31	4.415314970889476
4	32	5.231816153676679
4	33	5.0217527925116165
4	34	4.65186461725073
4	35	4.891391059526775
4	36	6.342450773735823
4	37	6.22241688178195
4	38	6.328759134722695
4	39	6.436440915313817
4	40	0.7208007338730944
4	41	10.961536699680568
4	42	10.04360186459407
4	43	9.455086800796195
4	44	10.059928075880919
4	45	8.633963697925449
4	46	11.99561203623447
4	47	12.399434046498271
4	48	11.901237129714307
4	49	11.109671593817055
4	50	11.588058330513672
4	51	10.540644109863788
4	52	10.098006996756997
4	53	10.12123916306409
4	54	10.146010738518754
4	55	10.466683668132639
4	56	10.195121884812359
5	1	2.9414120843036713
5	2	6.2524084434502925
5	3	4.294188212530635
5	4	4.405418648829837
5	5	3.9422374990911635
5	6	4.119403282406303
5	7	3.6987952273091125
5	8	3.908951066154323
5	9	3.5604787624666017
5	10	4.956999321383314
5	11	4.995442610188952
5	12	5.522153152734774
5	13	5.184134042298798
5	14	5.730451995437363
5	15	7.2884081649897965
5	16	7.736332624633196
5	17	8.843636703497626
5	18	9.459513365439925
5	19	7.769292610365174
5	20	7.074062695469905
5	21	7.295941127920351
5	22	7.565951021658162
5	23	6.8558215111881715
5	24	7.667261408689381
5	25	7.00262486335858
5	26	5.599909800127264
5	27	5.745232509913386
5	28	5.53125703505788
5	29	5.637569973818923
5	30	6.140652352006949
5	31	4.9469919895230205
5	32	4.308420708542105
5	33	4.782415318391532
5	34	4.540891532364604
5	35	4.950604310044376
5	36	5.814914541851767
5	37	6.259269883852344
5	38	6.826895780685426
5	39	7.846378803512579
5	40	8.888088518552184
5	41	11.069611233842057
5	42	10.464719877016432
5	43	10.422374585475282
5	44	10.712516067599346
5	45	10.047214215523791
5	46	11.506585121120594
5	47	11.930696986456866
5	48	11.854446239445156
5	49	11.787518384707326
5	50	10.687851291176198
5	51	10.640437070526314
5	52	10.582893125121387
5	53	11.293081813167607
5	54	11.068163947628484
5	55	11.612853440389365
5	56	11.024935549408632
6	1	3.036650179541766
6	2	6.170775790389072
6	3	3.7737800492653264
6	4	4.081609125020314
6	5	3.800740900451707
6	6	3.8704236905695666
6	7	3.5280469279893865
6	8	3.908270794045478
6	9	3.628505973350954
6	10	4.663802042471751
6	11	4.032857576175353
6	12	5.081336826204158
6	13	6.1718891443396195
6	14	5.162424784553011
6	15	5.906775511928572
6	16	6.975788406946119
6	17	8.147718336150692
6	18	8.849989555916117
6	19	7.267932066147491
6	20	6.450933443769223
6	21	6.6986622163557215
6	22	7.384998640705785
6	23	5.8939167492834095
6	24	7.204676374675774
6	25	6.255686087848371
6	26	5.473379187882365
6	27	4.755436591546044
6	28	5.645542749343594
6	29	5.183828477220278
6	30	6.21752310030627
6	31	4.846991989523019
6	32	4.9519581235080885
6	33	4.475612597303094
6	34	4.282388131004055
6	35	4.275774378071588
6	36	5.561173045253124
6	37	5.457909339634657
6	38	6.269752923542569
6	39	7.287195130043187
6	40	2.9561157294365366
6	41	10.869611233842061
6	42	9.815740285179697
6	43	9.025095673910656
6	44	10.764897019980296
6	45	9.119323059061205
6	46	10.926313012277056
6	47	12.449744605504485
6	48	10.643561885703665
6	49	11.743300697632499
6	50	11.056558774169392
6	51	11.533634349437879
6	52	11.643437342808461
6	53	11.67199337779346
6	54	11.171565308172703
6	55	11.444145957396174
6	56	10.249425345327001
7	1	2.836383156844839
7	2	5.873236213810777
7	3	3.927032004252929
7	4	3.7051071223500855
7	5	3.317048358014169
7	6	3.8854913998962886
7	7	3.4911405766638097
7	8	3.468541631352366
7	9	3.313037729978838
7	10	3.6612271807513608
7	11	4.108482218555665
7	12	4.773688533242115
7	13	4.900288915463019
7	14	4.302325604694147
7	15	5.062506963387655
7	16	5.541495063440497
7	17	6.765399053297362
7	18	7.4454501700683196
7	19	5.992612592563663
7	20	5.512157933565142
7	21	4.951570856447276
7	22	6.3729826193439685
7	23	4.911273224583812
7	24	5.956535997029388
7	25	5.188739683118261
7	26	5.378013938979066
7	27	4.897435447163055
7	28	4.573446621172703
7	29	4.867120485612421
7	30	5.290096054721676
7	31	3.65139786402235
7	32	3.7368141219822455
7	33	4.0749831866603365
7	34	3.3068016347233
7	35	4.193950994512555
7	36	4.978777470200672
7	37	5.42891830396805
7	38	5.955333697908394
7	39	6.476018322871724
7	40	6.401662172312751
7	41	9.764137268555007
7	42	9.906909463131253
7	43	9.651836089703146
7	44	10.379716779659873
7	45	8.98829120706807
7	46	10.686469411285259
7	47	11.21276196197978
7	48	10.999083533615167
7	49	12.066112065170167
7	50	10.5019144865478
7	51	10.562911480851195
7	52	10.484272742388853
7	53	10.818760495855638
7	54	10.6512525101563
7	55	10.243116012708015
7	56	9.72319990187859
8	1	2.645596788488376
8	2	5.926290117331966
8	3	4.100826019168437
8	4	4.322590366001549
8	5	4.090578047431713
8	6	4.190976153979179
8	7	3.2811906097044954
8	8	3.1349250921283485
8	9	3.746625948613783
8	10	4.138240302624297
8	11	5.174374789121131
8	12	6.211619242200861
8	13	5.801450059614819
8	14	4.967104232089596
8	15	6.313804990386622
8	16	7.186260474561049
8	17	7.8245890844500074
8	18	9.31319301911958
8	19	6.76236620343877
8	20	7.128319549726761
8	21	7.099404331383553
8	22	7.423959679666819
8	23	6.524508379875041
8	24	7.587896329324298
8	25	6.75269701343073
8	26	5.84464284486031
8	27	5.231523996204878
8	28	4.586379690180539
8	29	6.216502152751101
8	30	6.656092467447063
8	31	4.279170921701947
8	32	4.737569337690733
8	33	5.207523543499757
8	34	4.602940594413667
8	35	4.616116575556639
8	36	6.354308481245706
8	37	6.628389652972114
8	38	6.935698089487737
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115	25	5.00262486335858
115	26	4.742766942984403
115	27	4.088089652770535
115	28	4.845542749343597
115	29	4.361379497628445
115	30	4.7501761615307565
115	31	3.8327062752373067
115	32	4.356039756161149
115	33	3.8347962707724825
115	34	3.474224865697934
115	35	3.9553662148062827
115	36	4.7815812085184355
115	37	4.349746074328532
115	38	5.255467209256857
115	39	6.217807374941145
115	40	8.149993280456943
115	41	9.212468376699203
115	42	8.264719877016436
115	43	6.227136490237189
115	44	-1.106531551448274
115	45	6.7091189774285525
115	46	1.95896607350155
115	47	-7.935969680209803
115	48	1.8068271918261125
115	49	-3.3505768533879134
115	50	2.8545179578428623
115	51	4.09281802290727
115	52	5.035274077502336
115	53	4.488319908405707
115	54	4.191973471438008
115	55	4.574758202294131
115	56	6.05350697798006
116	1	2.912725911039182
116	2	5.243802591470946
116	3	3.753740708227703
116	4	3.8544146327655753
116	5	3.627148571954045
116	6	3.7737922669157697
116	7	3.2097533454961464
116	8	3.780781244008594
116	9	3.322383524371361
116	10	4.380177749381019
116	11	3.3563146698561965
116	12	3.581705648431843
116	13	4.028540238512225
116	14	4.090750331639313
116	15	-2.761161542411241
116	16	3.9932459923899337
116	17	6.2784043454941845
116	18	6.032433617878247
116	19	5.578357441116754
116	20	4.716977210673576
116	21	6.203456905315647
116	22	6.13370776290888
116	23	5.912849623637971
116	24	6.697668752349738
116	25	5.869406274718305
116	26	5.083902915445677
116	27	5.418210134698242
116	28	4.969122783767002
116	29	5.28599223428937
116	30	6.238300085799256
116	31	4.861621937887904
116	32	5.631082785421047
116	33	4.802266150290553
116	34	4.655636225422548
116	35	5.138326627887174
116	36	6.190014943458191
116	37	6.829436263657275
116	38	6.415191821993517
116	39	8.5802858603112
116	40	9.810062127272776
116	41	8.979766139177688
116	42	7.218248276327966
116	43	2.7588060255211815
116	44	3.9378746447651523
116	45	6.051459769166936
116	46	2.064129584689155
116	47	-6.719790678488632
116	48	-0.20866334173670964
116	49	-2.8860329635428172
116	50	1.7462563399426898
116	51	4.7518541674855825
116	52	4.800678552545367
116	53	4.609662421314482
116	54	4.582679151300315
116	55	3.9885275654610837
116	56	7.062801298117755
117	1	1.854622529772179
117	2	3.9344360932198796
117	3	3.151331069673489
117	4	2.5032681111954247
117	5	2.6559087740527616
117	6	2.4276982132818787
117	7	2.3795939984304653
117	8	2.6373688848947197
117	9	2.5481899759842648
117	10	3.1009317330576636
117	11	-0.7472609228371567
117	12	-8.165865280444951
117	13	-5.7112576627703255
117	14	-1.8678582964213177
117	15	-7.619886765885784
117	16	-6.574573673369876
117	17	-0.7775614531843971
117	18	-3.8089966191990925
117	19	-0.718572212983517
117	20	-1.1007452922413101
117	21	4.339873539594699
117	22	5.3711737559131585
117	23	4.290230113338712
117	24	4.853129304234695
117	25	4.233961268888532
117	26	4.067190906118043
117	27	3.904218685028596
117	28	3.546464408329772
117	29	4.805619128964853
117	30	4.926213027890203
117	31	3.471415952656656
117	32	2.9694038114606904
117	33	3.5154414320628042
117	34	3.9203078150066943
117	35	2.985780961350059
117	36	4.468677982711984
117	37	4.690299069720236
117	38	6.472517900501096
117	39	5.080480186001054
117	40	7.735246736678139
117	41	5.759012155501043
117	42	3.154120798675418
117	43	-5.527241389947143
117	44	-2.043858740388366
117	45	3.362575198626711
117	46	-1.1115408389408472
117	47	-0.382052629518558
117	48	0.9957672839920093
117	49	-0.7754616460146408
117	50	0.5144257919442445
117	51	3.637979313229849
117	52	3.2099284553825207
117	53	3.787859078912618
117	54	4.650498817060129
117	55	4.186739769114407
117	56	5.624013890422457
118	1	1.447364465256051
118	2	4.466694157736008
118	3	3.1156167839592044
118	4	2.849466267877453
118	5	2.6910470229006833
118	6	2.3551175681205905
118	7	2.0535571320710204
118	8	2.27561773282099
118	9	2.161669238657076
118	10	2.597475511859507
118	11	-1.2783669136205695
118	12	-7.092132561550944
118	13	-6.876580243415486
118	14	-5.013595623610257
118	15	-9.761591835010208
118	16	-6.633905470604901
118	17	-0.4492204393595145
118	18	-4.060724729798171
118	19	-1.2295169134443498
118	20	-1.3271277807205735
118	21	3.479274461253681
118	22	5.0457129264200695
118	23	4.19748817785484
118	24	4.143451884879855
118	25	3.2097677205014357
118	26	3.42490980012726
118	27	3.1380896527705318
118	28	3.145542749343594
118	29	4.186379497628447
118	30	3.6180333043878967
118	31	3.007706275237304
118	32	3.406039756161153
118	33	1.8276534136296299
118	34	3.4456534371265093
118	35	3.4517947862348493
118	36	3.8851526370898632
118	37	3.424746074328535
118	38	3.662610066399715
118	39	5.139235946369716
118	40	6.542850423314089
118	41	5.083896948127773
118	42	4.389719877016432
118	43	-0.7835777954770968
118	44	-1.467245837162558
118	45	3.3948332631428393
118	46	-2.3338910693555945
118	47	-3.103826823066946
118	48	-3.0681728081738875
118	49	-0.486291139102196
118	50	-0.6704820421571362
118	51	3.0463894514786958
118	52	3.8067026489309086
118	53	3.006177051262849
118	54	3.3169734714380077
118	55	3.706901059436987
118	56	5.417792692265774
119	1	1.5685183114098962
119	2	4.582078773120621
119	3	2.905177223519644
119	4	2.892598136009319
119	5	2.402310759164422
119	6	2.297425260428284
119	7	2.196963725477616
119	8	2.562430919634174
119	9	2.4608450628329024
119	10	3.510112874496869
119	11	-0.28001526526892206
119	12	-7.2360886055069855
119	13	-2.9938879357231762
119	14	-6.682002217016851
119	15	-8.87422919764757
119	16	-8.725938437637868
119	17	-0.6420775822166576
119	18	-5.0620983561717985
119	19	-2.2292421881696214
119	20	-2.241688220281013
119	21	4.787516219495444
119	22	5.34983380554095
119	23	3.953257408624072
119	24	3.4049903464183195
119	25	4.2905369512706635
119	26	5.15155815177561
119	27	4.903474268155151
119	28	4.588399892200737
119	29	5.464676200925151
119	30	5.497428908783501
119	31	3.5557831983142236
119	32	3.1890067891281824
119	33	3.8732578092340226
119	34	3.970928162401229
119	35	4.061959621399687
119	36	5.296965823903047
119	37	5.33546036004282
119	38	5.3060166598063105
119	39	6.209016166149937
119	40	8.010432840896502
119	41	4.588292552523377
119	42	3.444939657236212
119	43	-3.439896476795777
119	44	-1.961476606393326
119	45	3.248679416988992
119	46	-0.6003745858391092
119	47	0.35194240770228546
119	48	-5.968996983998064
119	49	-0.5692581720692296
119	50	0.7017707050956083
119	51	4.915894945984192
119	52	5.5374718797001385
119	53	4.601506721592518
119	54	3.9480174274819646
119	55	5.154977982513909
119	56	6.697463021936105
120	1	1.937749080640664
120	2	5.120540311582161
120	3	3.3205618389042613
120	4	3.0925981360093218
120	5	2.956156913010574
120	6	3.0820406450436693
120	7	2.643117571631457
120	8	2.9778155350187916
120	9	3.0146912166790543
120	10	3.5716513360353304
120	11	-0.37232295757661404
120	12	-5.1899347593531395
120	13	-4.563118704953947
120	14	-3.035848370863004
120	15	-0.08961381303218374
120	16	-5.602861514560942
120	17	1.1733070331679585
120	18	-1.785175279248719
120	19	0.4169116579842225
120	20	0.9429271643343711
120	21	5.279823911803135
120	22	6.026756882464024
120	23	5.553257408624074
120	24	5.528067269495242
120	25	5.075152335886049
120	26	5.828481228698692
120	27	4.965012729693612
120	28	4.957630661431512
120	29	4.649291585540535
120	30	5.8358904472450455
120	31	4.140398582929613
120	32	3.7428529429743413
120	33	4.411719347695563
120	34	4.724774316247384
120	35	3.9234980829381456
120	36	5.066196593133814
120	37	5.227768052350509
120	38	5.65986281365246
120	39	6.747477704611477
120	40	8.225817456281117
120	41	-2.242476678245854
120	42	-0.7396757273791721
120	43	-4.039896476795779
120	44	-13.530707375624097
120	45	-2.643628275318701
120	46	-8.215759201223726
120	47	-5.5557498999900226
120	48	-4.830535445536526
120	49	-13.830796633607692
120	50	-2.544383141058237
120	51	2.4851257152149593
120	52	3.4451641873924466
120	53	3.1091990292848273
120	54	2.8403251197896573
120	55	3.108824136360063
120	56	7.205155329628411
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1411.0
5	87.0
6	182.0
7	247.0
8	316.0
9	378.0
10	302.0
11	176.0
12	193.0
13	191.0
14	207.0
15	279.0
16	342.0
17	416.0
18	519.0
19	666.0
20	835.0
21	1092.0
22	1429.0
23	1672.0
24	1957.0
25	2195.0
26	2327.0
27	2360.0
28	2473.0
29	2641.0
30	2861.0
31	3221.0
32	3637.0
33	4152.0
34	4599.0
35	4797.0
36	5272.0
37	5800.0
38	5795.0
39	4638.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	99.36126541216322	0.37319324233159656	0.2296573798963671	0.035883965608807356
8	98.88903242475132	0.7363389742927271	0.2483170420129469	0.12631155894300192
9	94.00163630883176	2.2319826608678177	2.451592530393719	1.3147884999067017
10	44.34827541661284	24.432674503724755	22.38154702952533	8.837503050137077
11	29.066012143133964	25.111599133043388	16.34729937274828	29.475089351074367
12	29.38896783361323	29.107637543240177	17.84294305932337	23.66045156382322
13	20.872985115331065	16.714751180582468	21.2059883161808	41.20627538790566
14	21.41842139258494	34.975383599592355	22.502117153970918	21.104077853851784
15	36.72830602354028	19.55486960535426	20.703034848834527	23.013789522270944
16	26.617269059004293	34.578325363661165	20.755611079998275	18.048794497336264
17	25.458330342401837	20.136386476204148	35.73038547125116	18.674897710142847
18	22.154760366877664	20.49405043850206	21.213165109302558	36.13802408531772
19	23.732318517986645	21.647160192431965	20.366195160479645	34.25432612910175
20	22.03024379024839	22.705841766329346	35.55370285188593	19.71021159153634
21	21.702622400206693	20.641892376810347	23.917380757582283	33.73810446540068
22	20.331854913950252	20.4868736453803	38.45469290502232	20.726578535647132
23	21.392584937346594	21.886348304123786	35.540914897587164	21.180151860942455
24	22.055846672887807	21.09109180963319	22.27693632905032	34.57612518842868
25	23.691123903587478	22.090469286093686	33.16728635208659	21.051120458232244
26	37.7112984532307	22.58685317899151	18.58421060190438	21.117637765873415
27	22.7857737941337	34.81858140136171	19.209112585825505	23.186532218679076
28	28.92885852090032	31.656235645383557	18.1944763435921	21.220429490124022
29	33.90900463029536	28.069368151620605	19.302004544016565	18.71962267406747
30	31.8110010053138	22.350998132988654	27.426396668102832	18.411604193594716
31	31.6301039453282	30.14988801470166	19.504106127605812	18.715901912364323
32	33.73056325288231	28.350730089448522	20.1194561300234	17.799250527645764
33	31.380326219731263	29.38851764029604	21.026083207587842	18.20507293238485
34	22.881514406133576	23.167767085257267	36.11818351817489	17.832534990434272
35	21.117789152815323	26.545695059527237	35.578305630643584	16.75821015701386
36	28.38699485883333	25.80205072234828	27.30275440158543	18.508200017232962
37	30.28826108834735	24.163611530443536	26.468262526058517	19.0798648551506
38	31.834273543633433	24.496315910660833	24.798526364264333	18.8708841814414
39	28.5615699805909	24.540291855366256	20.460067572424702	26.438070591618146
40	24.359289755871668	19.482511613099287	24.943924286933996	31.214274344095045
41	20.471755592305684	18.168102822990107	22.28073229465866	39.079409290045554
42	20.365437502699745	31.393356467149502	22.18254596766065	26.0586600624901
43	17.676273284772407	26.110615265021735	28.95229320818818	27.260818242017677
44	19.612000576950813	26.363767488821576	29.061012548680225	24.963219385547383
45	27.40800437491905	23.456186049189057	27.28999669002835	21.845812885863545
46	19.223345720623396	31.39313801745744	23.517126148705096	25.86639011321407
47	17.654865979976343	27.254104273060385	21.06090770074151	34.03012204622176
48	25.559546042235308	24.552506823732223	24.081310156586696	25.80663697744577
49	19.708333933173055	28.700171314224836	22.748801520233794	28.842693232368312
50	19.159221373386853	31.40690865668206	24.10260837037996	25.331261599551127
51	19.509700836850215	30.482377173145895	25.76663257810362	24.24128941190027
52	20.010642428774826	35.41627716336128	24.368285562251017	20.204794845612874
53	19.172185430463575	32.78289663115462	25.704002303484017	22.340915634897783
54	18.76987724675848	24.585186144569644	29.560074256357122	27.084862352314754
55	19.603777931352223	22.802925593181296	30.98508408200875	26.60821239345773
56	19.87883323020247	24.165719301780086	30.339180613316834	25.61626685470061
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	2.0
5	4.0
6	5.5
7	7.0
8	43.0
9	79.0
10	228.5
11	378.0
12	363.5
13	307.5
14	266.0
15	223.0
16	180.0
17	187.5
18	195.0
19	197.5
20	200.0
21	195.0
22	193.5
23	197.0
24	221.5
25	246.0
26	279.0
27	312.0
28	350.0
29	421.5
30	455.0
31	533.5
32	612.0
33	712.0
34	812.0
35	931.0
36	1050.0
37	1131.5
38	1290.5
39	1368.0
40	1439.5
41	1511.0
42	1723.5
43	1936.0
44	2408.0
45	2880.0
46	4133.5
47	5904.0
48	6421.0
49	5425.0
50	4429.0
51	4211.5
52	3994.0
53	4183.0
54	4513.0
55	4654.0
56	4401.0
57	4148.0
58	4257.5
59	4367.0
60	4372.0
61	4377.0
62	4142.0
63	3309.0
64	2711.0
65	2348.5
66	1986.0
67	1690.0
68	1394.0
69	1156.0
70	918.0
71	797.5
72	601.0
73	525.0
74	391.5
75	258.0
76	195.5
77	133.0
78	103.5
79	76.5
80	79.0
81	51.5
82	24.0
83	15.0
84	6.0
85	3.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.48945729090413237
16	0.04306075873056883
17	0.020095020740932123
18	0.0
19	0.04880219322797801
20	0.14497122105958174
21	0.0
22	0.0
23	0.0
24	0.020095020740932123
25	0.014353586243522943
26	0.05741434497409177
27	0.07320328984196701
28	0.0071767931217614715
29	0.1822905452927414
30	0.055978986349739475
31	0.024401096613989006
32	0.028707172487045886
33	0.12057012444559273
34	0.21530379365284416
35	0.17367839354662762
36	0.05023755185233031
37	0.16363088317616156
38	0.2597999110077653
39	0.16363088317616156
40	0.8124129813833987
41	1.0549885888989363
42	0.31290818010880017
43	0.29137780074351577
44	0.4865865736554278
45	0.2612352696321176
46	0.3487921457176075
47	0.5038108771476553
48	0.08468615883678537
49	0.29568387661657264
50	0.2325280971450717
51	0.34735678709325524
52	0.19520877291191205
53	0.2999899524896295
54	0.258364552383413
55	0.3057313869870387
56	0.2554938351347084
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	69669.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.52481017382193
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.54005934718101	65.83415866454233
2	6.286115883179759	9.243709540828776
3	1.7569889114477588	3.875468285751195
4	0.7164610338903639	2.107106460549168
5	0.40410744963298456	1.4855961762046246
6	0.2869748555364673	1.2659863066787238
7	0.2030298297672966	1.0449410785284703
8	0.1307980634077776	0.7693522226528299
9	0.11908480399812588	0.7880118847694095
>10	0.5056223645166329	6.503609926940246
>50	0.025378728720912076	1.301870272287531
>100	0.02147430891769483	2.699909572406666
>500	0.001952209901608621	1.3277067275258725
>1k	0.001952209901608621	1.7525728803341516
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TAGTCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	1221	1.7525728803341516	No Hit
TAGTCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	925	1.3277067275258725	No Hit
TAGTCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	382	0.5483069945025765	No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	242	0.34735678709325524	No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	235	0.33730927672278915	No Hit
TAGTCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	178	0.2554938351347084	No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	145	0.20812700053108268	No Hit
TAGTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC	144	0.20669164190673042	No Hit
TAGTCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	118	0.16937231767357075	No Hit
TAGTCGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGAGCTCGTATGCCGT	116	0.16650160042486617	Illumina Single End Adapter 2 (95% over 24bp)
TAGTCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	111	0.15932480730310467	No Hit
TAGTCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC	108	0.1550187314300478	No Hit
TAGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	102	0.14640657968393403	No Hit
TAGTCGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	97	0.13922978656217255	No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA	87	0.1248762003186496	No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	78	0.11195797269947895	No Hit
TAGTCGGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC	76	0.10908725545077438	No Hit
TAGTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCA	73	0.10478117957771749	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.010047510370466061	0.0	0.0	0.0	0.0
12	0.010047510370466061	0.0	0.0	0.0	0.0
13	0.01578894486787524	0.0	0.0	0.0	0.0
14	0.06746185534455784	0.0	0.0	0.0	0.0
15	0.07176793121761472	0.0	0.0	0.0	0.0
16	0.08325080021243307	0.0	0.0	0.0	0.0
17	0.08899223470984226	0.0	0.0	0.0	0.0
18	0.09329831058289914	0.0	0.0	0.0	0.0
19	0.09616902783160372	0.0	0.0	0.0	0.0
20	0.1004751037046606	0.0	0.0	0.0	0.0
21	0.10621653820206979	0.0	0.0	0.0	0.0
22	0.10765189682642208	0.0	0.0	0.0	0.0
23	0.11339333132383125	0.0	0.0	0.0	0.0
24	0.11769940719688814	0.0	0.0	0.0	0.0
25	0.1277469175673542	0.0	0.0	0.0	0.0
26	0.13492371068911568	0.0	0.0	0.0	0.0
27	0.14210050381087716	0.0	0.0	0.0	0.0
28	0.15645409005440009	0.0	0.0	0.0	0.0
29	0.15788944867875238	0.0	0.0	0.0	0.0
30	0.16363088317616156	0.0	0.0	0.0	0.0
31	0.17080767629792304	0.0	0.0	0.0	0.0
32	0.17224303492227533	0.0	0.0	0.0	0.0
33	0.17654911079533223	0.0	0.0	0.0	0.0
34	0.17798446941968452	0.0	0.0	0.0	0.0
35	0.18229054529274138	0.0	0.0	0.0	0.0
36	0.18372590391709367	0.0	0.0	0.0	0.0
37	0.18372590391709367	0.0	0.0	0.0	0.0
38	0.18372590391709367	0.0	0.0	0.0	0.0
39	0.185161262541446	0.0	0.0	0.0	0.0
40	0.19090269703885515	0.0	0.0	0.0	0.0
41	0.19233805566320744	0.0	0.0	0.0	0.0
42	0.20238556603367353	0.0	0.0	0.0	0.0
43	0.2267866626476625	0.0	0.0	0.0	0.0
44	0.24257560751553775	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTCA	15	0.008154384	50.373093	41
GATTGAC	55	0.0	50.373093	41
CGATTGA	35	4.953654E-8	50.299927	40
GCGCAGA	15	0.008201416	50.299927	38
GCCGATT	35	4.953654E-8	50.299927	38
ATTTATC	15	0.008225008	50.263424	46
ACTATTA	15	0.008225008	50.263424	47
ACCGCTC	15	0.008225008	50.263424	45
AGACCAC	15	0.008225008	50.263424	37
AGTACAC	15	0.008225008	50.263424	47
AAGAGAC	15	0.008272342	50.190582	35
TAAGGGC	15	0.008272342	50.190582	14
GTGCATT	20	4.1084003E-4	50.117947	42
TTCCGCC	15	0.008319879	50.117947	44
CATTCCG	15	0.008319879	50.117947	42
GCATTTA	15	0.008319879	50.117947	44
TCATCCG	15	0.008319879	50.117947	42
ATGCAGT	15	0.008319879	50.117947	43
ATGATTT	15	0.008415562	49.973305	29
CTAAATG	15	0.008415562	49.973305	28
>>END_MODULE