FastQCFastQC Report
Fri 17 Jun 2016
SRR1525857_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525857_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12972
Sequences flagged as poor quality0
Sequence length40
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT2451.8886833179155103No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT1050.8094357076780758No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT1030.794017884674684No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT490.37773666358310204No Hit
TAGTCGGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT350.2698119025593586No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCCGTCGCCTATGCT270.20814061054579094No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC240.18501387604070307No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATCTGCT240.18501387604070307No Hit
TAGTCGGGGAAAAAAAAACAAAAAAAAAAAAAAAAAAAAA200.1541782300339192No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCCGTAGCCTCTGCT200.1541782300339192No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCCTCTGCT200.1541782300339192No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTCGCATATGCT190.14646931853222325No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.13105149552883133No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC170.13105149552883133No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAA160.12334258402713535No Hit
TAGTCGGGGTACCTGGTTCATCCTGCCCGTAGCCTATGCT160.12334258402713535No Hit
TAGTCGGGGTACCTGGTTCATCCTGCCCGTAGCATATGCT160.12334258402713535No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGACACGCAGGG160.12334258402713535No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCC160.12334258402713535No Hit
TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.11563367252543941No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGACACGCCGGG150.11563367252543941No Hit
TAGTCGGGGACACGCAGGGATGAGATGGGCAAAAAAAAAA150.11563367252543941No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCCGTAGCATCTGCT140.10792476102374346No Hit
TAGTCGGGGTACCTGGTTCATCCTGCCAGTAGCCTATGCT140.10792476102374346No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAA140.10792476102374346No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC140.10792476102374346No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCCGTAGCATATGCC130.10021584952204748No Hit
TAGTCGGGGACACGCAGGGATGAGATGGGGAAAAAAAAAA130.10021584952204748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTGC1400.034.31640620
CCAGTAG601.8189894E-1234.31640626
CCTGCCC650.034.31640622
CCTGCCA750.034.31640622
ATGGCCA200.002568336734.31640625
GCCAGTA650.034.31640625
TGCCCGT650.034.31640624
CCCGTAG406.506161E-834.31640626
TGCCAGT750.034.31640624
CTGCCCG650.034.31640623
CTGCCAG750.034.31640623
GCCCGTA503.2923708E-1034.31640625
CGTAGCC251.9470484E-433.91892228
TAGCATA503.7289283E-1033.91892230
CTGGTTG1000.033.91892213
GGAAGCA503.7289283E-1033.9189228
AGCATAT503.7289283E-1033.91892231
GGGAAGC503.7289283E-1033.9189227
GTTGATC1100.033.9189216
GGTTGAT1050.033.9189215
Produced by FastQC (version 0.11.5)