##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525857_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12972
Sequences flagged as poor quality	0
Sequence length	40
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.28060437866173	30.0	27.0	33.0	4.0	34.0
2	16.432778291705212	17.0	10.0	23.0	4.0	28.0
3	18.33973172987974	19.0	12.0	26.0	4.0	30.0
4	20.6399938328708	23.0	15.0	28.0	4.0	32.0
5	16.797332716620414	15.0	11.0	24.0	4.0	32.0
6	21.337573234659267	24.0	14.0	30.0	4.0	33.0
7	23.091658957755165	27.0	19.0	30.0	4.0	33.0
8	20.94041011409189	24.0	11.0	29.0	4.0	33.0
9	22.098211532531607	27.0	14.0	31.0	4.0	33.0
10	17.196885599753315	17.0	8.0	26.0	4.0	31.0
11	23.24398704902868	28.0	11.0	34.0	4.0	36.0
12	23.825238976256554	29.0	12.0	35.0	4.0	38.0
13	24.832485353068147	30.0	13.0	35.0	4.0	38.0
14	24.99444958371878	31.0	10.0	35.0	4.0	38.0
15	23.01703669441875	29.0	4.0	35.0	4.0	37.0
16	20.477721245760097	24.0	4.0	32.0	4.0	36.0
17	21.10699969164354	26.0	4.0	33.0	4.0	36.0
18	20.788467468393463	25.0	4.0	33.0	4.0	36.0
19	17.77967930928153	18.0	4.0	30.0	4.0	35.0
20	19.030835646006786	22.0	4.0	32.0	4.0	35.0
21	15.869179771816219	13.0	4.0	28.0	4.0	33.0
22	16.209374036386063	12.0	4.0	30.0	4.0	34.0
23	15.667668825161886	10.0	4.0	29.0	4.0	33.0
24	15.566142460684551	4.0	4.0	29.0	4.0	34.0
25	14.547718162195498	4.0	4.0	28.0	4.0	33.0
26	12.427536231884059	4.0	4.0	24.0	4.0	33.0
27	11.852143077397471	4.0	4.0	22.0	4.0	32.0
28	11.502158495220474	4.0	4.0	21.0	4.0	32.0
29	10.680157261794635	4.0	4.0	17.0	4.0	31.0
30	9.774514338575393	4.0	4.0	13.0	4.0	30.0
31	9.53322540857231	4.0	4.0	8.0	4.0	30.0
32	9.01981190255936	4.0	4.0	4.0	4.0	28.0
33	9.23704902867715	4.0	4.0	4.0	4.0	29.0
34	8.871261177921678	4.0	4.0	4.0	4.0	29.0
35	8.65263644773358	4.0	4.0	4.0	4.0	29.0
36	8.707755164970706	4.0	4.0	4.0	4.0	31.0
37	8.759713228492137	4.0	4.0	4.0	4.0	31.0
38	8.423835954363243	4.0	4.0	4.0	4.0	30.0
39	8.452436016034536	4.0	4.0	4.0	4.0	30.0
40	8.382053654024052	4.0	4.0	4.0	4.0	30.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-17.21887364234062
1	2	-12.033354624968144
1	3	-14.574271372181283
1	4	-15.97937911117575
1	5	-12.852210658396466
1	6	-16.469317160207048
1	7	-18.338186200543277
1	8	-16.46073167383682
1	9	-17.19929343610298
1	10	-12.113911113130396
1	11	-18.377262826471874
1	12	-19.07489630949105
1	13	-20.555940172290097
1	14	-19.381017817225384
1	15	-19.06545646386718
1	16	-16.306589014525272
1	17	-17.38907161685192
1	18	-16.592988764218717
1	19	-14.000012375080924
1	20	-14.761575195248017
1	21	-12.200512943481808
1	22	-12.178866352958558
1	23	-12.004718446189216
1	24	-11.641719587463102
1	25	-10.50783667089598
1	26	-7.533069163431431
1	27	-7.278678249586701
1	28	-7.00409739900663
1	29	-6.1074452673275825
1	30	-5.288285049976903
1	31	-5.51655886982676
1	32	-5.110130880635879
1	33	-5.299712337844873
1	34	-4.851903512738865
1	35	-4.813249236521367
1	36	-4.8876542119689645
1	37	-4.552488186572164
1	38	-4.253237683884294
1	39	-3.9240183665252726
1	40	-3.99077205899518
3	1	5.436298771452485
3	2	16.41492123710082
3	3	6.253314834715269
3	4	4.468896750893215
3	5	12.940892789879396
3	6	8.772062150137778
3	7	9.903193109801553
3	8	13.022026946852836
3	9	11.973120357000468
3	10	12.920571645490295
3	11	8.243426828700539
3	12	12.0975174836124
3	13	11.271646034606455
3	14	2.722430458636687
3	15	4.934543536132821
3	16	-1.3065890145252723
3	17	-2.3890716168519184
3	18	-4.592988764218717
3	19	-2.0000123750809244
3	20	-2.7615751952480174
3	21	11.799487056518192
3	22	9.821133647041442
3	23	3.995281553810784
3	24	3.3582804125368977
3	25	15.49216332910402
3	26	-7.533069163431431
3	27	-7.278678249586701
3	28	-7.00409739900663
3	29	-6.1074452673275825
3	30	-5.288285049976903
3	31	-5.51655886982676
3	32	-5.110130880635879
3	33	-5.299712337844873
3	34	-4.851903512738865
3	35	-4.813249236521367
3	36	-4.8876542119689645
3	37	-4.552488186572164
3	38	-4.253237683884294
3	39	-3.9240183665252726
3	40	-3.99077205899518
5	1	1.6862987714524849
5	2	8.16492123710082
5	3	0.8783148347152689
5	4	-8.406103249106785
5	5	9.440892789879396
5	6	0.1470621501377778
5	7	4.653193109801553
5	8	6.5220269468528365
5	9	-9.026879642999532
5	10	2.795571645490295
5	11	-0.7565731712994612
5	12	3.5975174836123998
5	13	2.896646034606455
5	14	2.472430458636687
5	15	-3.6904564638671786
5	16	4.068410985474728
5	17	6.860928383148082
5	18	9.282011235781283
5	19	6.499987624919076
5	20	8.113424804751983
5	21	9.549487056518192
5	22	13.196133647041442
5	23	12.620281553810784
5	24	13.983280412536898
5	25	13.99216332910402
5	26	3.5919308365685687
5	27	3.096321750413299
5	28	-0.2540973990066302
5	29	-1.2324452673275825
5	30	-0.6632850499769027
5	31	-0.1415588698267598
5	32	2.0148691193641213
5	33	0.9502876621551266
5	34	-0.10190351273886478
5	35	2.686750763478633
5	36	2.4873457880310355
5	37	2.4475118134278357
5	38	1.6217623161157064
5	39	1.3259816334747274
5	40	1.63422794100482
6	1	2.186298771452485
6	2	4.9982545704341526
6	3	0.5033148347152689
6	4	0.7188967508932151
6	5	2.6908927898793955
6	6	5.772062150137778
6	7	0.9865264431348848
6	8	-0.9779730531471635
6	9	5.639787023667136
6	10	1.9205716454902948
6	11	3.9934268287005388
6	12	1.0975174836123998
6	13	4.688312701273123
6	14	4.305763791970019
6	15	2.1012102027994892
6	16	7.52674431880806
6	17	7.0275950498147495
6	18	8.990344569114615
6	19	7.666654291585743
6	20	7.65509147141865
6	21	13.549487056518192
6	22	10.071133647041442
6	23	11.411948220477452
6	24	12.024947079203566
6	25	12.49216332910402
6	26	13.716930836568569
6	27	13.2213217504133
6	28	16.41256926766004
6	29	14.72588806600575
6	30	13.29504828335643
6	31	10.48344113017324
6	32	13.973202452697453
6	33	9.866954328821794
6	34	7.564763153927803
6	35	13.353417430145301
6	36	11.195679121364368
6	37	10.614178480094504
6	38	13.913428982782374
6	39	13.325981633474727
6	40	11.592561274338152
7	1	4.404040706936357
7	2	9.640727688713724
7	3	7.059766447618493
7	4	8.017283847667407
7	5	-0.8010426939915725
7	6	7.09464279529907
7	7	6.419322142059617
7	8	6.24783339846574
7	9	6.58602358280692
7	10	10.04960390355481
7	11	6.501491344829571
7	12	7.355581999741432
7	13	7.8845492604129035
7	14	4.915978845733463
7	15	6.418414503874757
7	16	7.725669049990856
7	17	7.159315479922274
7	18	2.9231402680393472
7	19	5.903213431370688
7	20	8.851328030558435
7	21	9.83174512103432
7	22	11.756617518009183
7	23	11.83399123123014
7	24	12.229248154472382
7	25	7.49216332910402
7	26	2.1443501914072787
7	27	2.172934653639105
7	28	2.96364453647724
7	29	3.7312644100917716
7	30	1.1633278532489033
7	31	2.0640862914635623
7	32	1.5995465387189594
7	33	0.6680295976389967
7	34	1.3739029388740391
7	35	0.8319120538012132
7	36	1.4671844977084554
7	37	1.7055763295568678
7	38	1.7467623161157064
7	39	0.9792074399263395
7	40	1.00922794100482
8	1	2.890844225997938
8	2	4.142193964373547
8	3	2.4351330165334524
8	4	-1.5311032491067849
8	5	-2.695470846484241
8	6	9.862971241046868
8	7	7.357738564347006
8	8	6.3856633104892
8	9	6.1549385388186515
8	10	2.284208009126658
8	11	7.334335919609629
8	12	6.6429720290669465
8	13	6.5443733073337285
8	14	5.358794095000324
8	15	-2.429092827503542
8	16	1.4206837127474543
8	17	-6.661798889579192
8	18	-0.5020796733096269
8	19	1.3636239885554389
8	20	0.14751571384289264
8	21	3.6176688747000085
8	22	3.9120427379505323
8	23	6.4498270083562375
8	24	7.812825867082351
8	25	-1.1442003072596165
8	26	-0.16943279979506798
8	27	-7.278678249586701
8	28	-7.00409739900663
8	29	-6.1074452673275825
8	30	-5.288285049976903
8	31	-5.51655886982676
8	32	-5.110130880635879
8	33	-5.299712337844873
8	34	-4.851903512738865
8	35	-4.813249236521367
8	36	-4.8876542119689645
8	37	-4.552488186572164
8	38	-4.253237683884294
8	39	-3.9240183665252726
8	40	-3.99077205899518
9	1	-8.897034561880847
9	2	-9.251745429565847
9	3	-10.746685165284731
9	4	-9.864436582440119
9	5	-9.392440543453937
9	6	-12.227937849862222
9	7	-11.430140223531781
9	8	-9.311306386480497
9	9	-10.360212976332866
9	10	-10.412761687843037
9	11	-11.089906504632795
9	12	-11.9024825163876
9	13	-11.061687298726879
9	14	-17.610902874696645
9	15	-11.065456463867179
9	16	-8.639922347858606
9	17	-7.722404950185252
9	18	-6.259655430885383
9	19	-7.000012375080924
9	20	-5.094908528581351
9	21	-12.200512943481808
9	22	-12.178866352958558
9	23	-12.004718446189216
9	24	-11.641719587463102
9	25	-10.50783667089598
9	26	-7.533069163431431
9	27	-7.278678249586701
9	28	-7.00409739900663
9	29	-6.1074452673275825
9	30	-5.288285049976903
9	31	-5.51655886982676
9	32	-5.110130880635879
9	33	-5.299712337844873
9	34	-4.851903512738865
9	35	-4.813249236521367
9	36	-4.8876542119689645
9	37	-4.552488186572164
9	38	-4.253237683884294
9	39	-3.9240183665252726
9	40	-3.99077205899518
10	1	2.6505844857382
10	2	0.057778379957962755
10	3	3.824743406143842
10	4	5.68318246517893
10	5	-1.7376786386920333
10	6	6.700633578709205
10	7	4.26033596694441
10	8	5.343455518281409
10	9	4.187406071286183
10	10	0.7062859312045795
10	11	9.314855400129108
10	12	8.990374626469546
10	13	8.37878889174931
10	14	9.329573315779541
10	15	6.327400678989964
10	16	10.729125271189012
10	17	11.003785526005228
10	18	11.049868378638426
10	19	13.96427333920479
10	20	12.41699623332341
10	21	12.513772770803907
10	22	14.642562218470015
10	23	13.602424410953642
10	24	16.393994698251184
10	25	12.706449043389735
10	26	8.681216550854284
10	27	7.471321750413299
10	28	5.960188315279085
10	29	9.606840446958133
10	30	4.961714950023097
10	31	6.269155415887527
10	32	0.9612976907926924
10	33	2.1288590907265554
10	34	1.6123822015468487
10	35	2.686750763478633
10	36	3.969488645173893
10	37	4.626083241999265
10	38	3.532476601829993
10	39	3.7188387763318698
10	40	5.152085083861962
11	1	-5.563701228547515
11	2	-6.58507876289918
11	3	-2.746685165284731
11	4	-1.5311032491067849
11	5	-2.0591072101206045
11	6	5.772062150137778
11	7	2.9031931098015527
11	8	6.0220269468528365
11	9	4.973120357000468
11	10	-1.0794283545097052
11	11	12.243426828700539
11	12	8.0975174836124
11	13	10.271646034606455
11	14	13.722430458636687
11	15	14.934543536132821
11	16	11.693410985474728
11	17	8.610928383148082
11	18	13.407011235781283
11	19	9.999987624919076
11	20	15.238424804751983
11	21	20.799487056518192
11	22	19.821133647041442
11	23	21.995281553810784
11	24	20.3582804125369
11	25	23.49216332910402
11	26	-7.533069163431431
11	27	-7.278678249586701
11	28	-7.00409739900663
11	29	-6.1074452673275825
11	30	-5.288285049976903
11	31	-5.51655886982676
11	32	-5.110130880635879
11	33	-5.299712337844873
11	34	-4.851903512738865
11	35	-4.813249236521367
11	36	-4.8876542119689645
11	37	-4.552488186572164
11	38	-4.253237683884294
11	39	-3.9240183665252726
11	40	-3.99077205899518
12	1	-1.5160821809284677
12	2	-0.7279359057563219
12	3	-1.413351831951399
12	4	-1.5311032491067849
12	5	-4.154345305358699
12	6	1.9625383406139676
12	7	2.712716919325363
12	8	3.8315507563766467
12	9	2.0207394046195155
12	10	3.253904978823627
12	11	3.7196173048910133
12	12	6.192755578850495
12	13	4.98593174889217
12	14	5.865287601493829
12	15	6.315495917085201
12	16	9.883887175950917
12	17	10.229976002195702
12	18	9.930820759590809
12	19	9.333320958252408
12	20	11.524139090466267
12	21	12.84710610413724
12	22	13.154466980374774
12	23	10.51909107762031
12	24	12.786851841108325
12	25	11.444544281484973
12	26	4.371692741330474
12	27	0.10227413136568053
12	28	7.472093077183844
12	29	3.7973166374343226
12	30	2.330762569070716
12	31	-0.5641779174458073
12	32	-1.252988023493022
12	33	0.12885909072655544
12	34	-0.32809398892934105
12	35	0.043893620621490825
12	36	0.7313934070786541
12	37	-1.2667739008578787
12	38	-0.11038054102715122
12	39	0.6474102049032986
12	40	-0.3241053923285131
13	1	4.345389680543395
13	2	-1.4941696719900879
13	3	-4.655776074375639
13	4	-5.803830521834058
13	5	7.577256426243032
13	6	0.5902439683195944
13	7	-1.2786250720166308
13	8	-1.250700325874437
13	9	-2.3905160066358953
13	10	-1.7157919908733419
13	11	-1.7565731712994612
13	12	1.6429720290669465
13	13	0.08982785278827166
13	14	0.8133395495457769
13	15	-1.1563655547762686
13	16	1.5115928036565442
13	17	3.1563829286026284
13	18	0.4979203266903731
13	19	0.8181694431008921
13	20	0.056606622933799144
13	21	2.1631234201545553
13	22	-1.7243208984131027
13	23	-4.550172991643761
13	24	-0.9144468601903757
13	25	-2.5987457618050716
13	26	-5.533069163431431
13	27	-2.551405522313974
13	28	-3.3677337626429935
13	29	-1.4710816309639458
13	30	-2.4701032317950844
13	31	-2.7892861425540323
13	32	-3.1101308806358787
13	33	-1.8451668832994192
13	34	-1.306448967284319
13	35	-3.3587037819759127
13	36	-2.069472393787146
13	37	-1.734306368390346
13	38	-1.4350558657024752
13	39	-1.1058365483434542
13	40	-1.1725902408133617
14	1	4.769632104785821
14	2	6.7482545704341526
14	3	9.586648168048601
14	4	7.468896750893215
14	5	0.27422612321272766
14	6	12.438728816804442
14	7	11.569859776468217
14	8	12.6886936135195
14	9	2.973120357000468
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111	22	-2.8455330196252238
111	23	-2.671385112855882
111	24	-2.3083862541297684
111	25	-1.174503337562646
111	26	-7.533069163431431
111	27	-7.278678249586701
111	28	-7.00409739900663
111	29	-6.1074452673275825
111	30	-5.288285049976903
111	31	-5.51655886982676
111	32	-5.110130880635879
111	33	-5.299712337844873
111	34	-4.851903512738865
111	35	-4.813249236521367
111	36	-4.8876542119689645
111	37	-4.552488186572164
111	38	-4.253237683884294
111	39	-3.9240183665252726
111	40	-3.99077205899518
112	1	-0.9137012285475166
112	2	0.4149212371008204
112	3	-3.4466851652847303
112	4	-1.6811032491067834
112	5	-3.959107210120605
112	6	0.5720621501377785
112	7	0.9531931098015534
112	8	-3.077973053147165
112	9	0.27312035700046877
112	10	2.920571645490295
112	11	0.5434268287005395
112	12	3.197517483612401
112	13	2.6716460346064537
112	14	4.022430458636688
112	15	2.08454353613282
112	16	-1.0065890145252716
112	17	-2.0890716168519177
112	18	-4.1429887642187175
112	19	-3.5000123750809244
112	20	-2.9615751952480167
112	21	-7.100512943481808
112	22	-5.478866352958558
112	23	-5.8047184461892165
112	24	-4.191719587463103
112	25	-2.8578366708959795
112	26	0.8169308365685684
112	27	-1.4786782495867001
112	28	-0.7040973990066295
112	29	-1.4074452673275832
112	30	0.11171495002309761
112	31	0.8334411301732398
112	32	-1.1601308806358785
112	33	-0.8497123378448741
112	34	0.8980964872611352
112	35	0.33675076347863353
112	36	1.2623457880310358
112	37	-0.1524881865721639
112	38	1.4967623161157064
112	39	0.7259816334747278
112	40	1.1092279410048196
113	1	5.436298771452485
113	2	8.41492123710082
113	3	4.253314834715269
113	4	-1.5311032491067849
113	5	10.940892789879396
113	6	-7.227937849862222
113	7	-0.09680689019844735
113	8	-1.9779730531471635
113	9	-1.026879642999532
113	10	-11.079428354509705
113	11	-1.7565731712994612
113	12	3.0975174836123998
113	13	-11.728353965393545
113	14	-16.277569541363313
113	15	-4.065456463867179
113	16	3.6934109854747277
113	17	5.610928383148082
113	18	-2.592988764218717
113	19	12.999987624919076
113	20	14.238424804751983
113	21	-3.200512943481808
113	22	14.821133647041442
113	23	15.995281553810784
113	24	-11.641719587463102
113	25	-10.50783667089598
113	26	-7.533069163431431
113	27	-7.278678249586701
113	28	-7.00409739900663
113	29	-6.1074452673275825
113	30	-5.288285049976903
113	31	-5.51655886982676
113	32	-5.110130880635879
113	33	-5.299712337844873
113	34	-4.851903512738865
113	35	-4.813249236521367
113	36	-4.8876542119689645
113	37	-4.552488186572164
113	38	-4.253237683884294
113	39	-3.9240183665252726
113	40	-3.99077205899518
114	1	-1.2065583714046575
114	2	2.6292069513865357
114	3	4.610457691858127
114	4	5.968896750893215
114	5	-2.773392924406318
114	6	-1.013652135576507
114	7	-1.953949747341305
114	8	2.9505983754242635
114	9	-6.812593928713818
114	10	1.2777145026331524
114	11	5.314855400129112
114	12	2.240374626469542
114	13	1.4859317488921704
114	14	2.151001887208114
114	15	-1.8511707495814633
114	16	4.83626812833187
114	17	4.182356954576655
114	18	2.692725521495568
114	19	4.357130482061933
114	20	4.66699623332341
114	21	5.513772770803907
114	22	1.5354193613271576
114	23	2.495281553810784
114	24	3.8582804125368977
114	25	3.6350204719611625
114	26	2.252645122282855
114	27	1.2213217504132992
114	28	2.567331172421941
114	29	2.249697589815275
114	30	4.068857807165955
114	31	4.197726844458954
114	32	5.532726262221264
114	33	3.271716233583698
114	34	1.7909536301182776
114	35	-0.02753495080708035
114	36	0.5409172166024643
114	37	2.090368956284978
114	38	2.1753337446871353
114	39	1.5045530620461562
114	40	1.50922794100482
116	1	-1.7637012285475144
116	2	-0.3850787628991803
116	3	-1.5466851652847318
116	4	-6.431103249106785
116	5	-5.759107210120604
116	6	0.8720621501377792
116	7	1.003193109801554
116	8	1.2220269468528357
116	9	-0.8268796429995326
116	10	6.120571645490294
116	11	5.143426828700537
116	12	3.697517483612401
116	13	3.271646034606455
116	14	5.522430458636688
116	15	5.034543536132823
116	16	1.9934109854747284
116	17	4.310928383148081
116	18	2.6070112357812825
116	19	-0.400012375080923
116	20	4.438424804751982
116	21	4.399487056518193
116	22	2.9211336470414437
116	23	1.1952815538107835
116	24	1.6582804125368984
116	25	3.6921633291040195
116	26	2.2669308365685694
116	27	1.6213217504132995
116	28	-1.7040973990066295
116	29	3.792554732672418
116	30	5.111714950023098
116	31	0.6834411301732395
116	32	2.489869119364121
116	33	2.5002876621551273
116	34	-0.5519035127388641
116	35	-2.4132492365213665
116	36	0.2123457880310351
116	37	1.0475118134278354
116	38	1.6467623161157068
116	39	2.175981633474727
116	40	2.2092279410048192
117	1	1.4362987714524849
117	2	-8.08507876289918
117	3	6.253314834715269
117	4	0.9688967508932151
117	5	-1.5591072101206045
117	6	8.272062150137778
117	7	7.403193109801553
117	8	-8.477973053147164
117	9	6.473120357000468
117	10	3.920571645490295
117	11	-0.2565731712994612
117	12	3.0975174836123998
117	13	4.771646034606455
117	14	-17.277569541363313
117	15	-5.565456463867179
117	16	-6.806589014525272
117	17	-14.389071616851918
117	18	-8.092988764218717
117	19	-5.500012375080924
117	20	-1.7615751952480174
117	21	-8.200512943481808
117	22	-5.678866352958558
117	23	1.4952815538107842
117	24	-11.641719587463102
117	25	-10.50783667089598
117	26	-7.533069163431431
117	27	-7.278678249586701
117	28	-7.00409739900663
117	29	-6.1074452673275825
117	30	-5.288285049976903
117	31	-5.51655886982676
117	32	-5.110130880635879
117	33	-5.299712337844873
117	34	-4.851903512738865
117	35	-4.813249236521367
117	36	-4.8876542119689645
117	37	-4.552488186572164
117	38	-4.253237683884294
117	39	-3.9240183665252726
117	40	-3.99077205899518
119	1	10.436298771452485
119	2	18.41492123710082
119	3	19.25331483471527
119	4	11.468896750893215
119	5	20.940892789879396
119	6	13.772062150137778
119	7	6.903193109801553
119	8	9.022026946852836
119	9	7.973120357000468
119	10	12.920571645490295
119	11	-6.756573171299461
119	12	9.0975174836124
119	13	10.271646034606455
119	14	10.722430458636687
119	15	11.934543536132821
119	16	-13.306589014525272
119	17	9.610928383148082
119	18	-16.592988764218717
119	19	-14.000012375080924
119	20	-14.761575195248017
119	21	-12.200512943481808
119	22	-12.178866352958558
119	23	-12.004718446189216
119	24	-11.641719587463102
119	25	-10.50783667089598
119	26	-7.533069163431431
119	27	-7.278678249586701
119	28	-7.00409739900663
119	29	-6.1074452673275825
119	30	-5.288285049976903
119	31	-5.51655886982676
119	32	-5.110130880635879
119	33	-5.299712337844873
119	34	-4.851903512738865
119	35	-4.813249236521367
119	36	-4.8876542119689645
119	37	-4.552488186572164
119	38	-4.253237683884294
119	39	-3.9240183665252726
119	40	-3.99077205899518
120	1	5.436298771452485
120	2	10.41492123710082
120	3	10.253314834715269
120	4	14.468896750893215
120	5	14.940892789879396
120	6	11.772062150137778
120	7	-0.09680689019844735
120	8	15.022026946852836
120	9	5.973120357000468
120	10	10.920571645490295
120	11	3.2434268287005388
120	12	5.0975174836124
120	13	5.271646034606455
120	14	-6.277569541363313
120	15	3.9345435361328214
120	16	-14.306589014525272
120	17	4.610928383148082
120	18	11.407011235781283
120	19	14.999987624919076
120	20	-14.761575195248017
120	21	-12.200512943481808
120	22	-12.178866352958558
120	23	-12.004718446189216
120	24	-11.641719587463102
120	25	-10.50783667089598
120	26	-7.533069163431431
120	27	-7.278678249586701
120	28	-7.00409739900663
120	29	-6.1074452673275825
120	30	-5.288285049976903
120	31	-5.51655886982676
120	32	-5.110130880635879
120	33	-5.299712337844873
120	34	-4.851903512738865
120	35	-4.813249236521367
120	36	-4.8876542119689645
120	37	-4.552488186572164
120	38	-4.253237683884294
120	39	-3.9240183665252726
120	40	-3.99077205899518
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	2370.0
5	32.0
6	178.0
7	114.0
8	85.0
9	160.0
10	299.0
11	435.0
12	524.0
13	484.0
14	587.0
15	640.0
16	712.0
17	654.0
18	699.0
19	647.0
20	591.0
21	507.0
22	494.0
23	493.0
24	487.0
25	471.0
26	403.0
27	311.0
28	225.0
29	155.0
30	114.0
31	54.0
32	29.0
33	11.0
34	4.0
35	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	99.0196835198765	0.6329602470088769	0.138942493245851	0.20841373986877654
8	96.76125848241826	1.2723627390499692	0.7325724861196792	1.2338062924120914
9	92.16230810769112	4.597701149425287	2.0365656098125435	1.2034251330710484
10	33.15343099460293	38.4425597532768	19.2212798766384	9.18272937548188
11	24.622263336416896	37.48843663274746	18.231575701510945	19.6577243293247
12	31.583005628807154	20.317680623024135	17.935075950343126	30.16423779782558
13	20.214324261814816	19.358569115719682	22.943489322334436	37.48361730013106
14	26.456984273820538	25.74005550416281	31.036077705827935	16.766882516188712
15	32.95559666975023	22.69503546099291	20.505704594511254	23.843663274745605
16	33.556891766882515	27.76749922910885	21.500154178230034	17.175454825778598
17	32.27721245760098	19.78877582485353	28.946962688868332	18.98704902867715
18	26.958063521430773	21.43077397471477	31.521739130434785	20.089423373419674
19	31.109393119443368	15.45419404715887	17.433320448395826	36.003092385001935
20	19.04872032069072	38.56768424298489	22.65649090348443	19.727104532839963
21	17.969472710453285	22.587110699969166	39.546716003700276	19.896700585877277
22	22.44410177332305	19.4834232845027	21.017733230531995	37.05474171164225
23	19.25686093123651	28.91612704286155	22.286463151403023	29.540548874498924
24	26.20461028448077	20.915889291496413	30.0285251715365	22.850975252486315
25	30.8303750578793	26.47785152029634	21.399907393116223	21.291866028708135
26	20.950977350294757	20.260626745268382	25.20943220601924	33.578963698417624
27	26.86969930609098	19.76869699306091	21.071703932151117	32.289899768697
28	25.790529076045043	28.189109979947556	20.661730680240627	25.358630263766774
29	31.953987493244806	23.81687639928974	21.330965799428704	22.898170308036747
30	18.75820526681597	22.395551780060234	32.488995289211516	26.35724766391227
31	16.224986479177932	36.49849339411265	25.511859692497872	21.764660434211542
32	26.05074419680728	24.03794246934526	24.73201203053906	25.1793013033084
33	15.583314056596501	26.193230010023903	25.129154136787722	33.09430179659187
34	13.660191181005244	32.88621646623497	27.582485353068147	25.871106999691644
35	13.723978411719354	27.486507324595223	37.00077101002313	21.7887432536623
36	13.50705419782592	35.594788374065224	26.728856680286796	24.169300747822064
37	14.608561511762439	27.527959892016966	36.2051677593521	21.65831083686849
38	23.417379905929526	27.342123525329633	27.534890893669516	21.705605675071325
39	14.230650632130743	26.42614862781375	25.832562442183164	33.51063829787234
40	13.946496029604502	27.993215634877807	35.841492560326884	22.21879577519081
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	2.0
8	2.0
9	4.5
10	7.0
11	15.5
12	24.0
13	24.0
14	43.0
15	62.0
16	84.0
17	106.0
18	106.0
19	116.5
20	127.0
21	130.0
22	133.0
23	133.0
24	145.0
25	157.0
26	165.0
27	173.0
28	173.0
29	186.5
30	200.0
31	215.5
32	231.0
33	231.0
34	265.5
35	300.0
36	335.5
37	371.0
38	371.0
39	431.0
40	491.0
41	502.0
42	513.0
43	513.0
44	609.0
45	705.0
46	765.0
47	825.0
48	825.0
49	857.5
50	890.0
51	1031.0
52	1172.0
53	1172.0
54	1224.0
55	1276.0
56	1183.0
57	1090.0
58	1090.0
59	1050.0
60	1010.0
61	974.0
62	938.0
63	938.0
64	870.5
65	803.0
66	693.0
67	583.0
68	583.0
69	478.0
70	373.0
71	292.0
72	211.0
73	211.0
74	158.0
75	105.0
76	79.0
77	53.0
78	53.0
79	40.0
80	27.0
81	18.0
82	9.0
83	9.0
84	5.5
85	2.0
86	2.0
87	2.0
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.13105149552883133
8	0.03083564600678384
9	0.06938020351526364
10	0.01541782300339192
11	0.0
12	0.023126734505087884
13	0.00770891150169596
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.28522972556275056
20	0.0
21	0.0
22	0.01541782300339192
23	0.0
24	0.00770891150169596
25	0.10792476102374346
26	0.6167129201356768
27	0.01541782300339192
28	0.04625346901017577
29	0.14646931853222325
30	0.1773049645390071
31	0.22355843354918284
32	0.0385445575084798
33	0.023126734505087884
34	0.0
35	0.01541782300339192
36	0.00770891150169596
37	0.05396238051187173
38	0.023126734505087884
39	0.0
40	0.00770891150169596
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	12972.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.66358310206599
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.65913538516278	82.90163428923836
2	2.7041451698985948	4.687018193031144
3	0.684931506849315	1.7807585568917668
4	0.311332503113325	1.0792476102374344
5	0.12453300124533001	0.5396238051187172
6	0.07116171499733144	0.37002775208140615
7	0.05337128624799858	0.32377428307123035
8	0.07116171499733144	0.4933703361085414
9	0.01779042874933286	0.13876040703052728
>10	0.2757516456146593	4.193647856922603
>50	0.0	0.0
>100	0.02668564312399929	3.4921369102682704
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT	245	1.8886833179155103	No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT	105	0.8094357076780758	No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT	103	0.794017884674684	No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT	49	0.37773666358310204	No Hit
TAGTCGGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT	35	0.2698119025593586	No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCCGTCGCCTATGCT	27	0.20814061054579094	No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC	24	0.18501387604070307	No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATCTGCT	24	0.18501387604070307	No Hit
TAGTCGGGGAAAAAAAAACAAAAAAAAAAAAAAAAAAAAA	20	0.1541782300339192	No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCCGTAGCCTCTGCT	20	0.1541782300339192	No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCCTCTGCT	20	0.1541782300339192	No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTCGCATATGCT	19	0.14646931853222325	No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17	0.13105149552883133	No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	17	0.13105149552883133	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAA	16	0.12334258402713535	No Hit
TAGTCGGGGTACCTGGTTCATCCTGCCCGTAGCCTATGCT	16	0.12334258402713535	No Hit
TAGTCGGGGTACCTGGTTCATCCTGCCCGTAGCATATGCT	16	0.12334258402713535	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGACACGCAGGG	16	0.12334258402713535	No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCC	16	0.12334258402713535	No Hit
TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15	0.11563367252543941	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGACACGCCGGG	15	0.11563367252543941	No Hit
TAGTCGGGGACACGCAGGGATGAGATGGGCAAAAAAAAAA	15	0.11563367252543941	No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCCGTAGCATCTGCT	14	0.10792476102374346	No Hit
TAGTCGGGGTACCTGGTTCATCCTGCCAGTAGCCTATGCT	14	0.10792476102374346	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAA	14	0.10792476102374346	No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC	14	0.10792476102374346	No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCCGTAGCATATGCC	13	0.10021584952204748	No Hit
TAGTCGGGGACACGCAGGGATGAGATGGGGAAAAAAAAAA	13	0.10021584952204748	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.015417823003391921	0.0	0.0	0.0	0.0
12	0.015417823003391921	0.0	0.0	0.0	0.0
13	0.015417823003391921	0.0	0.0	0.0	0.0
14	0.030835646006783842	0.0	0.0	0.0	0.0
15	0.0385445575084798	0.0	0.0	0.0	0.0
16	0.04625346901017576	0.0	0.0	0.0	0.0
17	0.04625346901017576	0.0	0.0	0.0	0.0
18	0.04625346901017576	0.0	0.0	0.0	0.0
19	0.04625346901017576	0.0	0.0	0.0	0.0
20	0.05396238051187172	0.0	0.0	0.0	0.0
21	0.05396238051187172	0.0	0.0	0.0	0.0
22	0.05396238051187172	0.0	0.0	0.0	0.0
23	0.05396238051187172	0.0	0.0	0.0	0.0
24	0.05396238051187172	0.0	0.0	0.0	0.0
25	0.05396238051187172	0.0	0.0	0.0	0.0
26	0.05396238051187172	0.0	0.0	0.0	0.0
27	0.05396238051187172	0.0	0.0	0.0	0.0
28	0.05396238051187172	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTGC	140	0.0	34.316406	20
CCAGTAG	60	1.8189894E-12	34.316406	26
CCTGCCC	65	0.0	34.316406	22
CCTGCCA	75	0.0	34.316406	22
ATGGCCA	20	0.0025683367	34.316406	25
GCCAGTA	65	0.0	34.316406	25
TGCCCGT	65	0.0	34.316406	24
CCCGTAG	40	6.506161E-8	34.316406	26
TGCCAGT	75	0.0	34.316406	24
CTGCCCG	65	0.0	34.316406	23
CTGCCAG	75	0.0	34.316406	23
GCCCGTA	50	3.2923708E-10	34.316406	25
CGTAGCC	25	1.9470484E-4	33.918922	28
TAGCATA	50	3.7289283E-10	33.918922	30
CTGGTTG	100	0.0	33.918922	13
GGAAGCA	50	3.7289283E-10	33.918922	8
AGCATAT	50	3.7289283E-10	33.918922	31
GGGAAGC	50	3.7289283E-10	33.918922	7
GTTGATC	110	0.0	33.91892	16
GGTTGAT	105	0.0	33.91892	15
>>END_MODULE