FastQCFastQC Report
Fri 17 Jun 2016
SRR1525856_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525856_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1490
Sequences flagged as poor quality0
Sequence length40
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCGGGGTACCTGGTTGATCCTCCCAGTACCATATGCT50.33557046979865773No Hit
TAGTCGGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.33557046979865773No Hit
TAGTCGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT40.2684563758389262No Hit
TAGTCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT40.2684563758389262No Hit
TAGTCGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.20134228187919465No Hit
TAGTCGGGGCTGGTTTCGTTTTTTTTTTTTTTTTTTTTTT30.20134228187919465No Hit
TAGTCGGGGGGGCTGGTGAGATGGCCCCGTGGGTAAGAGC30.20134228187919465No Hit
TAGTCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT20.1342281879194631No Hit
TAGTCGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTT20.1342281879194631No Hit
TAGTCGGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT20.1342281879194631No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1342281879194631No Hit
TAGTCGGGGCAAAAAAAAACAAAAAAAAACAAAAAAAACA20.1342281879194631No Hit
TAGTCGGGAAAAAAAAAACAAAAAAAAAAACAAAAAAAAA20.1342281879194631No Hit
TAGTCGGGGTACCTGGTTCATCCCCCCAGTACCATATGCT20.1342281879194631No Hit
TAGTCGGGGTACCTGGTTGATCCTCCCCGTACCATATGCT20.1342281879194631No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT20.1342281879194631No Hit
TAGTCGGGGGCGTCGACTTTTTTTTTTTTTTTTTTTTTTT20.1342281879194631No Hit
TAGTCGGGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTT20.1342281879194631No Hit
TAGTCGGGGAAAAAAAAACAAAAAAAAAAAAAAACACCCC20.1342281879194631No Hit
TAGTCGGGGTACCTGGTTGATCCTCCCAGTAGCATCTGCT20.1342281879194631No Hit
TAGTCGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1342281879194631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGGG1300.034.0000043
CGGGGGA251.1973548E-434.05
TCGGGGT200.001971197834.04
TCGGGGG600.034.04
TCGGGGA353.9735096E-734.04
TAGTCGG1500.031.7333341
AGTCGGG1500.031.7333342
Produced by FastQC (version 0.11.5)