##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525856_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1490 Sequences flagged as poor quality 0 Sequence length 40 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 15.664429530201343 4.0 4.0 28.0 4.0 32.0 2 10.843624161073825 4.0 4.0 19.0 4.0 26.0 3 10.126174496644296 4.0 4.0 17.0 4.0 23.0 4 11.301342281879194 4.0 4.0 19.0 4.0 26.0 5 8.796644295302013 4.0 4.0 13.0 4.0 15.0 6 10.384563758389262 4.0 4.0 19.0 4.0 23.0 7 12.302684563758389 4.0 4.0 23.0 4.0 29.0 8 12.07785234899329 4.0 4.0 23.0 4.0 27.0 9 11.400671140939597 4.0 4.0 22.0 4.0 27.0 10 9.296644295302013 4.0 4.0 15.0 4.0 23.0 11 11.153020134228187 4.0 4.0 18.0 4.0 31.0 12 11.116107382550336 4.0 4.0 18.0 4.0 31.0 13 11.322147651006711 4.0 4.0 20.0 4.0 33.0 14 10.91744966442953 4.0 4.0 17.0 4.0 31.0 15 10.425503355704699 4.0 4.0 15.0 4.0 31.0 16 8.211409395973154 4.0 4.0 4.0 4.0 23.0 17 7.654362416107382 4.0 4.0 4.0 4.0 20.0 18 7.384563758389262 4.0 4.0 4.0 4.0 19.0 19 7.706711409395973 4.0 4.0 4.0 4.0 22.0 20 7.839597315436242 4.0 4.0 4.0 4.0 22.0 21 8.10469798657718 4.0 4.0 4.0 4.0 24.0 22 7.82751677852349 4.0 4.0 4.0 4.0 22.0 23 8.102013422818793 4.0 4.0 4.0 4.0 23.0 24 7.721476510067114 4.0 4.0 4.0 4.0 22.0 25 7.831543624161074 4.0 4.0 4.0 4.0 22.0 26 8.540939597315436 4.0 4.0 4.0 4.0 31.0 27 8.619463087248322 4.0 4.0 4.0 4.0 30.0 28 8.49731543624161 4.0 4.0 4.0 4.0 29.0 29 8.481879194630872 4.0 4.0 4.0 4.0 30.0 30 8.376510067114094 4.0 4.0 4.0 4.0 28.0 31 8.521476510067114 4.0 4.0 4.0 4.0 31.0 32 8.442953020134228 4.0 4.0 4.0 4.0 31.0 33 8.521476510067114 4.0 4.0 4.0 4.0 31.0 34 8.583221476510067 4.0 4.0 4.0 4.0 32.0 35 8.412080536912752 4.0 4.0 4.0 4.0 31.0 36 8.37248322147651 4.0 4.0 4.0 4.0 32.0 37 8.259731543624161 4.0 4.0 4.0 4.0 31.0 38 8.202013422818792 4.0 4.0 4.0 4.0 31.0 39 8.16979865771812 4.0 4.0 4.0 4.0 31.0 40 8.179194630872484 4.0 4.0 4.0 4.0 31.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.5048237476808914 1 2 -6.510698824984539 1 3 -5.683271490414349 1 4 -6.567377860235004 1 5 -5.319820655534942 1 6 -5.673964131106988 1 7 -7.365491651205936 1 8 -7.009338280766853 1 9 -6.498423005565861 1 10 -4.245856524427953 1 11 -5.27612863327149 1 12 -5.762368583797155 1 13 -6.118181818181819 1 14 -5.855936920222636 1 15 -5.389424860853433 1 16 -3.639208410636983 1 17 -3.3871057513914664 1 18 -2.4683982683982677 1 19 -3.1348794063079755 1 20 -3.355040197897342 1 21 -2.810265924551639 1 22 -2.92581941867656 1 23 -3.356493506493507 1 24 -2.4529684601113173 1 25 -2.766110080395795 1 26 -2.8152442795299937 1 27 -2.884075448361161 1 28 -3.153555967841683 1 29 -3.174551638837353 1 30 -2.9847247990105137 1 31 -2.8267779839208416 1 32 -2.9816635745207174 1 33 -2.8445269016697594 1 34 -2.8501546072974646 1 35 -2.796691403834262 1 36 -2.4308905380333954 1 37 -2.3592455163883734 1 38 -2.2055658627087196 1 39 -1.6211502782931353 1 40 -1.8581632653061222 5 1 4.3285095856524425 5 2 8.989301175015461 5 3 2.8167285095856514 5 4 7.932622139764996 5 5 -5.319820655534942 5 6 -5.673964131106988 5 7 -7.365491651205936 5 8 -7.009338280766853 5 9 -6.498423005565861 5 10 -4.245856524427953 5 11 -5.27612863327149 5 12 -5.762368583797155 5 13 -6.118181818181819 5 14 -5.855936920222636 5 15 -5.389424860853433 5 16 -3.639208410636983 5 17 -3.3871057513914664 5 18 -2.4683982683982677 5 19 -3.1348794063079755 5 20 -3.355040197897342 5 21 -2.810265924551639 5 22 -2.92581941867656 5 23 -3.356493506493507 5 24 -2.4529684601113173 5 25 -2.766110080395795 5 26 -2.8152442795299937 5 27 -2.884075448361161 5 28 -3.153555967841683 5 29 -3.174551638837353 5 30 -2.9847247990105137 5 31 -2.8267779839208416 5 32 -2.9816635745207174 5 33 -2.8445269016697594 5 34 -2.8501546072974646 5 35 -2.796691403834262 5 36 -2.4308905380333954 5 37 -2.3592455163883734 5 38 -2.2055658627087196 5 39 -1.6211502782931353 5 40 -1.8581632653061222 9 1 11.828509585652442 9 2 9.489301175015461 9 3 17.31672850958565 9 4 4.432622139764996 9 5 5.680179344465058 9 6 12.326035868893012 9 7 17.634508348794064 9 8 15.990661719233147 9 9 16.50157699443414 9 10 11.754143475572047 9 11 15.72387136672851 9 12 18.237631416202845 9 13 25.881818181818183 9 14 23.144063079777364 9 15 26.61057513914657 9 16 -3.639208410636983 9 17 -3.3871057513914664 9 18 -2.4683982683982677 9 19 -3.1348794063079755 9 20 -3.355040197897342 9 21 -2.810265924551639 9 22 -2.92581941867656 9 23 -3.356493506493507 9 24 -2.4529684601113173 9 25 -2.766110080395795 9 26 -2.8152442795299937 9 27 -2.884075448361161 9 28 -3.153555967841683 9 29 -3.174551638837353 9 30 -2.9847247990105137 9 31 -2.8267779839208416 9 32 -2.9816635745207174 9 33 -2.8445269016697594 9 34 -2.8501546072974646 9 35 -2.796691403834262 9 36 -2.4308905380333954 9 37 -2.3592455163883734 9 38 -2.2055658627087196 9 39 -1.6211502782931353 9 40 -1.8581632653061222 10 1 16.82850958565244 10 2 20.489301175015463 10 3 18.31672850958565 10 4 9.432622139764996 10 5 7.680179344465058 10 6 15.326035868893012 10 7 20.634508348794064 10 8 17.990661719233145 10 9 20.50157699443414 10 10 -4.245856524427953 10 11 -5.27612863327149 10 12 -5.762368583797155 10 13 -6.118181818181819 10 14 -5.855936920222636 10 15 -5.389424860853433 10 16 -3.639208410636983 10 17 -3.3871057513914664 10 18 -2.4683982683982677 10 19 -3.1348794063079755 10 20 -3.355040197897342 10 21 -2.810265924551639 10 22 -2.92581941867656 10 23 -3.356493506493507 10 24 -2.4529684601113173 10 25 -2.766110080395795 10 26 -2.8152442795299937 10 27 -2.884075448361161 10 28 -3.153555967841683 10 29 -3.174551638837353 10 30 -2.9847247990105137 10 31 -2.8267779839208416 10 32 -2.9816635745207174 10 33 -2.8445269016697594 10 34 -2.8501546072974646 10 35 -2.796691403834262 10 36 -2.4308905380333954 10 37 -2.3592455163883734 10 38 -2.2055658627087196 10 39 -1.6211502782931353 10 40 -1.8581632653061222 12 1 4.3285095856524425 12 2 1.739301175015461 12 3 4.066728509585651 12 4 3.4326221397649963 12 5 -0.06982065553494188 12 6 8.076035868893012 12 7 8.134508348794064 12 8 7.740661719233147 12 9 6.501576994434139 12 10 7.504143475572047 12 11 11.97387136672851 12 12 10.487631416202845 12 13 16.631818181818183 12 14 1.3940630797773643 12 15 -5.389424860853433 12 16 -3.639208410636983 12 17 -3.3871057513914664 12 18 -2.4683982683982677 12 19 -3.1348794063079755 12 20 -3.355040197897342 12 21 -2.810265924551639 12 22 -2.92581941867656 12 23 -3.356493506493507 12 24 -2.4529684601113173 12 25 -2.766110080395795 12 26 -2.8152442795299937 12 27 -2.884075448361161 12 28 -3.153555967841683 12 29 -3.174551638837353 12 30 -2.9847247990105137 12 31 -2.8267779839208416 12 32 -2.9816635745207174 12 33 -2.8445269016697594 12 34 -2.8501546072974646 12 35 -2.796691403834262 12 36 -2.4308905380333954 12 37 -2.3592455163883734 12 38 -2.2055658627087196 12 39 -1.6211502782931353 12 40 -1.8581632653061222 13 1 5.3285095856524425 13 2 4.489301175015461 13 3 5.816728509585651 13 4 4.432622139764996 13 5 -0.06982065553494188 13 6 7.826035868893012 13 7 7.634508348794064 13 8 6.490661719233147 13 9 4.251576994434139 13 10 6.254143475572047 13 11 10.72387136672851 13 12 12.737631416202845 13 13 12.131818181818181 13 14 13.394063079777364 13 15 -2.139424860853433 13 16 1.360791589363017 13 17 0.6128942486085336 13 18 3.2816017316017323 13 19 0.8651205936920245 13 20 -0.35504019789734187 13 21 3.939734075448361 13 22 3.82418058132344 13 23 3.393506493506493 13 24 4.297031539888683 13 25 3.983889919604205 13 26 4.184755720470006 13 27 4.865924551638839 13 28 4.596444032158317 13 29 4.575448361162647 13 30 4.765275200989486 13 31 4.923222016079158 13 32 4.768336425479283 13 33 4.905473098330241 13 34 3.3998453927025354 13 35 3.703308596165738 13 36 -2.4308905380333954 13 37 -2.3592455163883734 13 38 -2.2055658627087196 13 39 -1.6211502782931353 13 40 -1.8581632653061222 14 1 5.0785095856524425 14 2 2.989301175015461 14 3 5.066728509585651 14 4 4.682622139764996 14 5 -0.06982065553494188 14 6 5.826035868893012 14 7 10.384508348794064 14 8 4.240661719233147 14 9 7.251576994434139 14 10 4.504143475572047 14 11 13.72387136672851 14 12 12.237631416202845 14 13 13.381818181818181 14 14 11.394063079777364 14 15 8.610575139146567 14 16 8.360791589363018 14 17 6.112894248608534 14 18 6.281601731601732 14 19 2.3651205936920245 14 20 1.1449598021026581 14 21 1.689734075448361 14 22 -0.6758194186765598 14 23 3.393506493506493 14 24 -0.2029684601113173 14 25 3.983889919604205 14 26 -1.3152442795299937 14 27 -0.1340754483611608 14 28 -1.4035559678416831 14 29 1.575448361162647 14 30 -1.7347247990105137 14 31 4.923222016079158 14 32 4.268336425479283 14 33 -2.8445269016697594 14 34 -2.8501546072974646 14 35 -2.796691403834262 14 36 -2.4308905380333954 14 37 -2.3592455163883734 14 38 -2.2055658627087196 14 39 -1.6211502782931353 14 40 -1.8581632653061222 16 1 -2.1714904143475575 16 2 5.989301175015461 16 3 5.316728509585651 16 4 2.4326221397649963 16 5 -0.8198206555349419 16 6 5.326035868893012 16 7 1.6345083487940641 16 8 1.990661719233147 16 9 3.0015769944341386 16 10 1.7541434755720466 16 11 8.22387136672851 16 12 3.737631416202845 16 13 -1.618181818181819 16 14 7.144063079777364 16 15 8.110575139146567 16 16 -3.639208410636983 16 17 -3.3871057513914664 16 18 -2.4683982683982677 16 19 -3.1348794063079755 16 20 -3.355040197897342 16 21 -2.810265924551639 16 22 -2.92581941867656 16 23 -3.356493506493507 16 24 -2.4529684601113173 16 25 -2.766110080395795 16 26 -2.8152442795299937 16 27 -2.884075448361161 16 28 -3.153555967841683 16 29 -3.174551638837353 16 30 -2.9847247990105137 16 31 -2.8267779839208416 16 32 -2.9816635745207174 16 33 -2.8445269016697594 16 34 -2.8501546072974646 16 35 -2.796691403834262 16 36 -2.4308905380333954 16 37 -2.3592455163883734 16 38 -2.2055658627087196 16 39 -1.6211502782931353 16 40 -1.8581632653061222 17 1 6.8285095856524425 17 2 10.489301175015461 17 3 9.316728509585651 17 4 15.432622139764996 17 5 5.680179344465058 17 6 9.326035868893012 17 7 11.634508348794064 17 8 15.990661719233147 17 9 13.501576994434139 17 10 18.754143475572047 17 11 1.7238713667285097 17 12 25.237631416202845 17 13 22.881818181818183 17 14 23.144063079777364 17 15 17.61057513914657 17 16 27.360791589363018 17 17 27.612894248608534 17 18 28.531601731601732 17 19 25.865120593692026 17 20 27.644959802102658 17 21 8.18973407544836 17 22 26.07418058132344 17 23 22.643506493506493 17 24 4.547031539888683 17 25 23.233889919604206 17 26 -2.8152442795299937 17 27 -2.884075448361161 17 28 -3.153555967841683 17 29 -3.174551638837353 17 30 -2.9847247990105137 17 31 -2.8267779839208416 17 32 -2.9816635745207174 17 33 -2.8445269016697594 17 34 -2.8501546072974646 17 35 -2.796691403834262 17 36 -2.4308905380333954 17 37 -2.3592455163883734 17 38 -2.2055658627087196 17 39 -1.6211502782931353 17 40 -1.8581632653061222 18 1 1.3285095856524425 18 2 -4.010698824984539 18 3 1.8167285095856514 18 4 3.9326221397649963 18 5 0.18017934446505812 18 6 0.32603586889301184 18 7 1.6345083487940641 18 8 2.990661719233147 18 9 5.001576994434139 18 10 2.2541434755720466 18 11 10.72387136672851 18 12 6.237631416202845 18 13 9.881818181818181 18 14 8.644063079777364 18 15 10.110575139146567 18 16 12.360791589363018 18 17 11.112894248608534 18 18 13.531601731601732 18 19 10.865120593692025 18 20 10.644959802102658 18 21 -2.810265924551639 18 22 -2.92581941867656 18 23 -3.356493506493507 18 24 -2.4529684601113173 18 25 -2.766110080395795 18 26 -2.8152442795299937 18 27 -2.884075448361161 18 28 -3.153555967841683 18 29 -3.174551638837353 18 30 -2.9847247990105137 18 31 -2.8267779839208416 18 32 -2.9816635745207174 18 33 -2.8445269016697594 18 34 -2.8501546072974646 18 35 -2.796691403834262 18 36 -2.4308905380333954 18 37 -2.3592455163883734 18 38 -2.2055658627087196 18 39 -1.6211502782931353 18 40 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19 40 -1.8581632653061222 22 1 13.828509585652442 22 2 7.489301175015461 22 3 15.316728509585651 22 4 5.432622139764996 22 5 1.6801793444650581 22 6 12.326035868893012 22 7 15.634508348794064 22 8 15.990661719233147 22 9 16.50157699443414 22 10 4.754143475572047 22 11 1.7238713667285097 22 12 6.237631416202845 22 13 18.881818181818183 22 14 16.144063079777364 22 15 19.61057513914657 22 16 18.360791589363018 22 17 21.612894248608534 22 18 16.531601731601732 22 19 17.865120593692026 22 20 13.644959802102658 22 21 28.18973407544836 22 22 28.07418058132344 22 23 27.643506493506493 22 24 19.547031539888682 22 25 22.233889919604206 22 26 28.184755720470008 22 27 28.11592455163884 22 28 28.846444032158317 22 29 28.825448361162646 22 30 29.015275200989485 22 31 29.17322201607916 22 32 29.018336425479283 22 33 28.15547309833024 22 34 29.149845392702534 22 35 29.203308596165737 22 36 24.569109461966605 22 37 24.640754483611627 22 38 15.79443413729128 22 39 10.378849721706864 22 40 30.14183673469388 23 1 19.82850958565244 23 2 20.489301175015463 23 3 23.31672850958565 23 4 16.432622139764995 23 5 25.68017934446506 23 6 19.326035868893012 23 7 23.634508348794064 23 8 21.990661719233145 23 9 22.50157699443414 23 10 -4.245856524427953 23 11 -5.27612863327149 23 12 -5.762368583797155 23 13 -6.118181818181819 23 14 -5.855936920222636 23 15 -5.389424860853433 23 16 -3.639208410636983 23 17 -3.3871057513914664 23 18 -2.4683982683982677 23 19 -3.1348794063079755 23 20 -3.355040197897342 23 21 -2.810265924551639 23 22 -2.92581941867656 23 23 -3.356493506493507 23 24 -2.4529684601113173 23 25 -2.766110080395795 23 26 -2.8152442795299937 23 27 -2.884075448361161 23 28 -3.153555967841683 23 29 -3.174551638837353 23 30 -2.9847247990105137 23 31 -2.8267779839208416 23 32 -2.9816635745207174 23 33 -2.8445269016697594 23 34 -2.8501546072974646 23 35 -2.796691403834262 23 36 -2.4308905380333954 23 37 -2.3592455163883734 23 38 -2.2055658627087196 23 39 -1.6211502782931353 23 40 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-5.855936920222636 117 15 -5.389424860853433 117 16 -3.639208410636983 117 17 -3.3871057513914664 117 18 -2.4683982683982677 117 19 -3.1348794063079755 117 20 -3.355040197897342 117 21 -2.810265924551639 117 22 -2.92581941867656 117 23 -3.356493506493507 117 24 -2.4529684601113173 117 25 -2.766110080395795 117 26 -2.8152442795299937 117 27 -2.884075448361161 117 28 -3.153555967841683 117 29 -3.174551638837353 117 30 -2.9847247990105137 117 31 -2.8267779839208416 117 32 -2.9816635745207174 117 33 -2.8445269016697594 117 34 -2.8501546072974646 117 35 -2.796691403834262 117 36 -2.4308905380333954 117 37 -2.3592455163883734 117 38 -2.2055658627087196 117 39 -1.6211502782931353 117 40 -1.8581632653061222 120 1 17.82850958565244 120 2 -6.510698824984539 120 3 -5.683271490414349 120 4 -6.567377860235004 120 5 -5.319820655534942 120 6 -5.673964131106988 120 7 -7.365491651205936 120 8 -7.009338280766853 120 9 -6.498423005565861 120 10 -4.245856524427953 120 11 -5.27612863327149 120 12 -5.762368583797155 120 13 -6.118181818181819 120 14 -5.855936920222636 120 15 -5.389424860853433 120 16 -3.639208410636983 120 17 -3.3871057513914664 120 18 -2.4683982683982677 120 19 -3.1348794063079755 120 20 -3.355040197897342 120 21 -2.810265924551639 120 22 -2.92581941867656 120 23 -3.356493506493507 120 24 -2.4529684601113173 120 25 -2.766110080395795 120 26 -2.8152442795299937 120 27 -2.884075448361161 120 28 -3.153555967841683 120 29 -3.174551638837353 120 30 -2.9847247990105137 120 31 -2.8267779839208416 120 32 -2.9816635745207174 120 33 -2.8445269016697594 120 34 -2.8501546072974646 120 35 -2.796691403834262 120 36 -2.4308905380333954 120 37 -2.3592455163883734 120 38 -2.2055658627087196 120 39 -1.6211502782931353 120 40 -1.8581632653061222 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 864.0 5 12.0 6 26.0 7 19.0 8 18.0 9 32.0 10 47.0 11 73.0 12 46.0 13 24.0 14 16.0 15 17.0 16 15.0 17 11.0 18 8.0 19 26.0 20 25.0 21 36.0 22 40.0 23 32.0 24 25.0 25 17.0 26 27.0 27 16.0 28 11.0 29 2.0 30 3.0 31 1.0 32 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 97.7088948787062 0.8086253369272237 0.40431266846361186 1.078167115902965 8 96.24161073825503 2.0805369127516777 0.738255033557047 0.9395973154362416 9 88.0456682337139 6.044325050369376 2.887844190732035 3.022162525184688 10 27.181208053691275 35.77181208053691 16.44295302013423 20.604026845637584 11 23.154362416107382 35.369127516778526 16.308724832214764 25.16778523489933 12 31.409395973154364 22.348993288590606 16.845637583892618 29.395973154362416 13 18.926174496644297 24.22818791946309 23.557046979865774 33.288590604026844 14 20.28206850235057 30.423102753525853 29.28139691067831 20.013431833445267 15 28.187919463087248 21.409395973154364 22.684563758389263 27.71812080536913 16 29.664429530201343 25.57046979865772 23.221476510067113 21.543624161073826 17 20.80536912751678 25.369127516778523 28.993288590604028 24.832214765100673 18 19.19463087248322 27.91946308724832 28.389261744966444 24.496644295302016 19 19.784656796769852 24.09152086137281 21.265141318977122 34.85868102288022 20 14.630872483221477 31.946308724832218 24.697986577181208 28.7248322147651 21 12.751677852348994 29.261744966442954 34.02684563758389 23.95973154362416 22 14.496644295302014 27.181208053691275 25.100671140939596 33.22147651006711 23 16.711409395973153 29.06040268456376 23.557046979865774 30.671140939597315 24 17.315436241610737 28.389261744966444 28.389261744966444 25.906040268456376 25 14.90222521915037 23.533378287255562 22.31962238705327 39.244774106540795 26 13.593539703903096 23.014804845222073 23.687752355316285 39.703903095558545 27 19.147496617050066 23.545331529093367 25.16914749661705 32.138024357239516 28 17.183288409703504 27.695417789757414 23.8544474393531 31.266846361185983 29 22.282241728561782 26.67116812964213 25.725860904794057 25.320729237002027 30 13.978494623655912 24.193548387096776 33.266129032258064 28.561827956989248 31 13.642473118279568 28.225806451612907 28.49462365591398 29.63709677419355 32 14.29530201342282 25.63758389261745 29.664429530201343 30.402684563758392 33 11.006711409395974 21.20805369127517 32.48322147651007 35.30201342281879 34 8.187919463087248 28.523489932885905 34.29530201342282 28.993288590604028 35 8.657718120805368 24.966442953020135 40.33557046979866 26.040268456375838 36 8.131720430107526 29.368279569892476 36.76075268817204 25.739247311827956 37 11.238223418573352 26.17765814266487 40.84791386271871 21.736204576043068 38 13.89261744966443 25.503355704697988 34.83221476510067 25.771812080536915 39 10.335570469798657 22.550335570469798 34.36241610738255 32.75167785234899 40 8.590604026845638 23.758389261744966 40.20134228187919 27.449664429530202 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 1.0 12 1.0 13 1.0 14 7.0 15 13.0 16 15.5 17 18.0 18 18.0 19 22.5 20 27.0 21 35.0 22 43.0 23 43.0 24 43.0 25 43.0 26 46.0 27 49.0 28 49.0 29 51.5 30 54.0 31 47.5 32 41.0 33 41.0 34 43.5 35 46.0 36 42.5 37 39.0 38 39.0 39 45.0 40 51.0 41 52.5 42 54.0 43 54.0 44 61.5 45 69.0 46 77.5 47 86.0 48 86.0 49 86.5 50 87.0 51 88.5 52 90.0 53 90.0 54 104.5 55 119.0 56 109.5 57 100.0 58 100.0 59 95.0 60 90.0 61 82.5 62 75.0 63 75.0 64 70.0 65 65.0 66 68.5 67 72.0 68 72.0 69 60.0 70 48.0 71 45.0 72 42.0 73 42.0 74 33.0 75 24.0 76 21.0 77 18.0 78 18.0 79 15.0 80 12.0 81 8.0 82 4.0 83 4.0 84 4.0 85 4.0 86 3.0 87 2.0 88 2.0 89 1.5 90 1.0 91 1.5 92 2.0 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.4026845637583893 8 0.0 9 0.06711409395973154 10 0.0 11 0.0 12 0.0 13 0.0 14 0.06711409395973154 15 0.0 16 0.0 17 0.0 18 0.0 19 0.2684563758389262 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.4697986577181208 26 0.2684563758389262 27 0.8053691275167786 28 0.4026845637583893 29 0.6040268456375839 30 0.1342281879194631 31 0.1342281879194631 32 0.0 33 0.0 34 0.0 35 0.0 36 0.1342281879194631 37 0.2684563758389262 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 1490.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.71812080536913 #Duplication Level Percentage of deduplicated Percentage of total 1 98.5576923076923 96.30872483221476 2 0.9615384615384616 1.8791946308724832 3 0.20604395604395606 0.6040268456375839 4 0.13736263736263737 0.5369127516778524 5 0.13736263736263737 0.6711409395973155 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TAGTCGGGGTACCTGGTTGATCCTCCCAGTACCATATGCT 5 0.33557046979865773 No Hit TAGTCGGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.33557046979865773 No Hit TAGTCGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT 4 0.2684563758389262 No Hit TAGTCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT 4 0.2684563758389262 No Hit TAGTCGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3 0.20134228187919465 No Hit TAGTCGGGGCTGGTTTCGTTTTTTTTTTTTTTTTTTTTTT 3 0.20134228187919465 No Hit TAGTCGGGGGGGCTGGTGAGATGGCCCCGTGGGTAAGAGC 3 0.20134228187919465 No Hit TAGTCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.1342281879194631 No Hit TAGTCGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 2 0.1342281879194631 No Hit TAGTCGGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT 2 0.1342281879194631 No Hit TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.1342281879194631 No Hit TAGTCGGGGCAAAAAAAAACAAAAAAAAACAAAAAAAACA 2 0.1342281879194631 No Hit TAGTCGGGAAAAAAAAAACAAAAAAAAAAACAAAAAAAAA 2 0.1342281879194631 No Hit TAGTCGGGGTACCTGGTTCATCCCCCCAGTACCATATGCT 2 0.1342281879194631 No Hit TAGTCGGGGTACCTGGTTGATCCTCCCCGTACCATATGCT 2 0.1342281879194631 No Hit TAGTCGGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT 2 0.1342281879194631 No Hit TAGTCGGGGGCGTCGACTTTTTTTTTTTTTTTTTTTTTTT 2 0.1342281879194631 No Hit TAGTCGGGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTT 2 0.1342281879194631 No Hit TAGTCGGGGAAAAAAAAACAAAAAAAAAAAAAAACACCCC 2 0.1342281879194631 No Hit TAGTCGGGGTACCTGGTTGATCCTCCCAGTAGCATCTGCT 2 0.1342281879194631 No Hit TAGTCGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.1342281879194631 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGGGG 130 0.0 34.000004 3 CGGGGGA 25 1.1973548E-4 34.0 5 TCGGGGT 20 0.0019711978 34.0 4 TCGGGGG 60 0.0 34.0 4 TCGGGGA 35 3.9735096E-7 34.0 4 TAGTCGG 150 0.0 31.733334 1 AGTCGGG 150 0.0 31.733334 2 >>END_MODULE