FastQCFastQC Report
Fri 17 Jun 2016
SRR1525855_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525855_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4692
Sequences flagged as poor quality0
Sequence length40
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG861.8329070758738277No Hit
TAGTCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT721.5345268542199488No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA511.0869565217391304No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC310.6606990622335891No Hit
TAGTCGGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT200.4262574595055414No Hit
TAGTCGGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG200.4262574595055414No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT170.36231884057971014No Hit
TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.319693094629156No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGTCACAGATCC120.2557544757033248No Hit
TAGTCGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGA110.23444160272804776No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAA100.2131287297527707No Hit
TAGTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT100.2131287297527707No Hit
TAGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG90.1918158567774936No Hit
TAGTCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC90.1918158567774936No Hit
TAGTCGGGGCAGAGTGCAGTGCTTAGTCGGGGTCACAGAT80.17050298380221654No Hit
TAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.17050298380221654No Hit
TAGTCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC80.17050298380221654No Hit
TAGTCGGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCG80.17050298380221654No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT70.14919011082693948No Hit
TAGTCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.14919011082693948No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC70.14919011082693948No Hit
TAGTCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG60.1278772378516624No Hit
TAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.1278772378516624No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGTCACAGATCCTC60.1278772378516624No Hit
TAGTCGGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT60.1278772378516624No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGTCACAGATCCT50.10656436487638535No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA50.10656436487638535No Hit
TAGTCGGGGTCACAGATACTCTTCTGAGATGAGTTTTTGT50.10656436487638535No Hit
TAGTCGGGGACTAATCCTAGCCCTAGCCCTACACAAATAT50.10656436487638535No Hit
TAGTCGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG50.10656436487638535No Hit
TAGTCGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAG50.10656436487638535No Hit
TAGTCGGGGTGTCTGCTTGTCTGTGTATACCACCTGTGTG50.10656436487638535No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTA200.002514300234.0000045
CGGGGGA358.1432154E-734.0000045
GTCGGGT200.002514300234.0000043
GGTAAAA200.002514300234.0000048
CGGGGGG850.034.05
CGGGGGC301.1919767E-534.05
CGGGGAT453.7271093E-934.05
CGGGGAG301.1919767E-534.05
TCGGGGC453.7271093E-934.04
TCGGGGA1300.034.04
TCGGGGG1750.033.0285724
TAGTCGG4800.032.93751
AGTCGGG4750.032.9263152
GTCGGGG4400.032.8409083
TGAAGGA556.9485395E-1030.9090925
CGGGGAA501.0435542E-830.5999985
TCTGAAG501.0435542E-830.59999823
AAGGAGA501.0435542E-830.59999827
TCGGGGT900.030.2222214
GGAGATT402.31396E-629.75000229
Produced by FastQC (version 0.11.5)