##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525855_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4692
Sequences flagged as poor quality	0
Sequence length	40
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	19.921355498721226	29.0	4.0	33.0	4.0	33.0
2	12.042625745950554	4.0	4.0	21.0	4.0	30.0
3	11.296675191815856	4.0	4.0	19.0	4.0	27.0
4	10.821824381926684	4.0	4.0	17.0	4.0	27.0
5	11.552642796248934	4.0	4.0	21.0	4.0	30.0
6	10.497016197783461	4.0	4.0	17.0	4.0	28.0
7	12.127024722932651	4.0	4.0	24.0	4.0	31.0
8	11.748508098891731	4.0	4.0	23.0	4.0	31.0
9	11.584185848252345	4.0	4.0	23.0	4.0	31.0
10	10.22847399829497	4.0	4.0	17.0	4.0	29.0
11	10.327365728900256	4.0	4.0	17.0	4.0	30.0
12	10.162617220801364	4.0	4.0	16.0	4.0	30.0
13	9.503623188405797	4.0	4.0	12.0	4.0	28.0
14	9.932651321398124	4.0	4.0	12.0	4.0	30.0
15	9.668584825234442	4.0	4.0	10.0	4.0	30.0
16	8.351875532821824	4.0	4.0	4.0	4.0	26.0
17	7.988917306052856	4.0	4.0	4.0	4.0	23.0
18	7.788576300085252	4.0	4.0	4.0	4.0	23.0
19	7.4884910485933505	4.0	4.0	4.0	4.0	21.0
20	7.961636828644501	4.0	4.0	4.0	4.0	25.0
21	7.019607843137255	4.0	4.0	4.0	4.0	19.0
22	7.091645353793691	4.0	4.0	4.0	4.0	20.0
23	7.11231884057971	4.0	4.0	4.0	4.0	20.0
24	6.827152600170503	4.0	4.0	4.0	4.0	18.0
25	7.120843989769821	4.0	4.0	4.0	4.0	21.0
26	7.20076726342711	4.0	4.0	4.0	4.0	21.0
27	7.365515771526002	4.0	4.0	4.0	4.0	22.0
28	7.529838022165388	4.0	4.0	4.0	4.0	24.0
29	6.958439897698209	4.0	4.0	4.0	4.0	19.0
30	7.205669224211424	4.0	4.0	4.0	4.0	21.0
31	7.6658141517476555	4.0	4.0	4.0	4.0	26.0
32	7.602088661551577	4.0	4.0	4.0	4.0	26.0
33	7.548593350383632	4.0	4.0	4.0	4.0	25.0
34	7.60464620630861	4.0	4.0	4.0	4.0	26.0
35	7.361892583120205	4.0	4.0	4.0	4.0	24.0
36	7.307544757033248	4.0	4.0	4.0	4.0	25.0
37	7.2591645353793695	4.0	4.0	4.0	4.0	25.0
38	7.141730605285592	4.0	4.0	4.0	4.0	23.0
39	7.015771526001705	4.0	4.0	4.0	4.0	22.0
40	7.09079283887468	4.0	4.0	4.0	4.0	23.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	12.86255613924153
1	2	12.401615675211179
1	3	2.4093482983932404
1	4	12.600932276213177
1	5	2.433329685295977
1	6	1.2766112164988588
1	7	-0.6824318627689365
1	8	-3.512272808340221
1	9	-6.7227957747620675
1	10	1.1259140360263942
1	11	-1.9163954958898763
1	12	0.8044842485853714
1	13	-5.8264271932811305
1	14	-6.036616566953647
1	15	-5.370702611994746
1	16	-4.034551777810204
1	17	-3.9162637612075795
1	18	-3.393417724597498
1	19	-3.762870275791623
1	20	-3.417265350130516
1	21	-3.043900886878416
1	22	-2.9607491447379077
1	23	-2.768770773546054
1	24	-2.965506185450006
1	25	-3.447580135220586
1	26	-3.2049252557679537
1	27	-3.7128893266533725
1	28	-3.845398608881754
1	29	-3.0072069004653272
1	30	-3.363840248390809
1	31	-3.7421153752614433
1	32	-3.8030307083677863
1	33	-3.9234730936978144
1	34	-3.7386420383611405
1	35	-3.464525674076235
1	36	-3.7333264425961046
1	37	-3.63839924120823
1	38	-2.961266760704963
1	39	-3.1732959612173097
1	40	-3.2811785592684473
4	1	-3.63744386075847
4	2	-5.223384324788821
4	3	3.7843482983932404
4	4	4.600932276213177
4	5	3.933329685295977
4	6	0.4016112164988588
4	7	-5.4324318627689365
4	8	4.112727191659779
4	9	4.4022042252379325
4	10	0.8759140360263942
4	11	-0.9163954958898763
4	12	2.4294842485853714
4	13	1.7985728067188695
4	14	6.213383433046353
4	15	1.629297388005254
4	16	4.465448222189796
4	17	6.4587362387924205
4	18	5.106582275402502
4	19	6.737129724208377
4	20	2.582734649869484
4	21	0.9560991131215841
4	22	0.5392508552620923
4	23	4.731229226453946
4	24	5.159493814549994
4	25	0.5524198647794138
4	26	6.920074744232046
4	27	3.6621106733466275
4	28	6.154601391118246
4	29	6.867793099534673
4	30	10.761159751609192
4	31	3.8828846247385567
4	32	4.571969291632214
4	33	9.326526906302185
4	34	4.5113579616388595
4	35	3.160474325923765
4	36	7.641673557403895
4	37	2.61160075879177
4	38	6.913733239295037
4	39	7.07670403878269
4	40	6.968821440731553
5	1	14.695889472574866
5	2	10.151615675211179
5	3	17.07601496505991
5	4	19.517598942879843
5	5	14.849996351962645
5	6	18.026611216498857
5	7	3.5675681372310635
5	8	7.321060524993111
5	9	16.02720422523793
5	10	19.542580702693062
5	11	14.166937837443456
5	12	10.22115091525204
5	13	15.006906140052202
5	14	13.296716766379685
5	15	17.629297388005256
5	16	-0.03455177781020424
5	17	-2.2495970945409125
5	18	2.273248942069168
5	19	1.9037963908750433
5	20	0.24940131653615083
5	21	2.456099113121584
5	22	0.5392508552620923
5	23	2.231229226453946
5	24	2.534493814549994
5	25	3.71908653144608
5	26	4.295074744232046
5	27	2.9537773400132936
5	28	3.321268057784912
5	29	3.659459766201339
5	30	2.969493084942525
5	31	4.091217958071891
5	32	6.030302624965548
5	33	5.4098602396355195
5	34	2.9280246283055256
5	35	5.535474325923765
5	36	1.4333402240705615
5	37	1.86160075879177
5	38	5.038733239295037
5	39	4.493370705449356
5	40	3.385488107398219
6	1	2.5768418535272453
6	2	8.080187103782606
6	3	0.9093482983932404
6	4	1.9937894190703194
6	5	-0.5309560289897366
6	6	-2.90196021207257
6	7	3.9961395658024905
6	8	9.059155763088352
6	9	9.527204225237933
6	10	1.7330568931692518
6	11	3.047890218395837
6	12	8.054484248585371
6	13	7.1735728067188695
6	14	7.39195486161778
6	15	8.200725959433827
6	16	8.536876793618369
6	17	11.798021953078136
6	18	8.035153703973931
6	19	10.094272581351234
6	20	14.868448935583768
6	21	7.527527684550155
6	22	7.039250855262092
6	23	8.231229226453946
6	24	8.177350957407135
6	25	6.4095627219222715
6	26	10.080789029946331
6	27	10.144253530489486
6	28	11.583172819689672
6	29	10.278507385248957
6	30	11.636159751609192
6	31	8.115027481881414
6	32	11.625540720203642
6	33	8.219384049159327
6	34	8.832786533067432
6	35	4.964045754495194
6	36	7.409530700261038
6	37	8.504457901648912
6	38	6.181590382152179
6	39	8.683846895925548
6	40	6.433107155017266
7	1	2.291127567812957
7	2	-0.7769557533602498
7	3	-6.662080273035331
7	4	-5.577639152358252
7	5	-5.5309560289897375
7	6	-6.044817354929712
7	7	-7.57528900562608
7	8	-5.512272808340221
7	9	-4.187081489047782
7	10	-5.124085963973606
7	11	-4.094966924461305
7	12	-2.8026586085574854
7	13	-2.540712907566845
7	14	-5.608045138382218
7	15	-3.0849883262804605
7	16	-1.463123206381633
7	17	-1.7734066183504362
7	18	-0.9648462960260691
7	19	-2.6200131329344796
7	20	-1.274408207273373
7	21	-1.9010437440212726
7	22	-0.10360628759505097
7	23	-1.1973422021174827
7	24	-2.536934756878577
7	25	-0.16186584950630056
7	26	-1.4906395414822393
7	27	-1.5700321837962292
7	28	-1.273970037453183
7	29	-1.864349757608184
7	30	0.35044546589490544
7	31	0.11502748188141343
7	32	-0.23160213693921516
7	33	-0.06633023655495762
7	34	-0.7386420383611405
7	35	-2.464525674076235
7	36	-0.30475501402467575
7	37	-1.0669706697796588
7	38	1.1815903821521792
7	39	1.255275467354119
7	40	-1.138321416411304
9	1	15.86255613924153
9	2	10.651615675211179
9	3	23.90934829839324
9	4	25.85093227621318
9	5	25.183329685295977
9	6	19.526611216498857
9	7	23.567568137231063
9	8	3.487727191659779
9	9	22.52720422523793
9	10	3.875914036026394
9	11	1.3336045041101237
9	12	21.05448424858537
9	13	12.17357280671887
9	14	13.963383433046353
9	15	16.629297388005256
9	16	-0.03455177781020424
9	17	0.08373623879242054
9	18	-0.3934177245974979
9	19	2.237129724208377
9	20	10.582734649869483
9	21	14.956099113121585
9	22	14.039250855262093
9	23	2.231229226453946
9	24	20.034493814549993
9	25	15.552419864779413
9	26	6.795074744232046
9	27	25.28711067334663
9	28	24.154601391118245
9	29	26.992793099534673
9	30	10.636159751609192
9	31	12.257884624738557
9	32	19.196969291632215
9	33	28.076526906302185
9	34	12.26135796163886
9	35	12.535474325923765
9	36	19.266673557403895
9	37	28.36160075879177
9	38	10.038733239295038
9	39	22.82670403878269
9	40	9.718821440731553
10	1	3.418111694797087
10	2	-1.4594954358999317
10	3	3.9093482983932404
10	4	2.0731544984353985
10	5	6.9611074630737555
10	6	-0.6956110057233627
10	7	8.678679248342174
10	8	7.709949413882001
10	9	10.638315336349043
10	10	6.431469591581951
10	11	6.555826726332345
10	12	2.498928693029816
10	13	4.729128362274425
10	14	4.296716766379687
10	15	8.184852943560811
10	16	6.521003777745351
10	17	5.639291794347976
10	18	10.051026719846945
10	19	10.126018613097266
10	20	9.360512427647262
10	21	8.400543557566028
10	22	4.594806410817648
10	23	9.897895893120614
10	24	5.145604925661105
10	25	8.441308753668302
10	26	5.572852522009825
10	27	5.731555117791072
10	28	8.599045835562688
10	29	3.548348655090228
10	30	4.191715307164746
10	31	5.702329069183001
10	32	8.419191513854436
10	33	6.4098602396355195
10	34	7.928024628305526
10	35	6.7576965481459865
10	36	8.933340224070562
10	37	10.139378536569549
10	38	9.26095546151726
10	39	9.382259594338247
10	40	9.052154774064885
11	1	10.084778361463751
11	2	8.651615675211179
11	3	3.7982371872821297
11	4	9.073154498435398
11	5	10.62777412974042
11	6	0.6377223276099695
11	7	13.900901470564396
11	8	5.265504969437558
11	9	5.749426447460154
11	10	7.875914036026394
11	11	6.889160059665681
11	12	8.054484248585371
11	13	7.1735728067188695
11	14	5.852272321935242
11	15	5.29596405467192
11	16	8.521003777745353
11	17	6.305958461014642
11	18	9.27324894206917
11	19	6.570463057541711
11	20	11.13829020542504
11	21	6.178321335343806
11	22	4.372584188595426
11	23	6.342340337565057
11	24	5.70116048121666
11	25	5.107975420334969
11	26	9.350630299787603
11	27	12.50933289556885
11	28	9.710156946673802
11	29	11.215015321756894
11	30	11.191715307164749
11	31	11.035662402516335
11	32	10.641413736076657
11	33	7.187638017413296
11	34	8.483580183861081
11	35	4.646585437034876
11	36	10.044451335181673
11	37	10.028267425458438
11	38	7.260955461517258
11	39	10.82670403878269
11	40	10.941043662953774
12	1	4.86255613924153
12	2	5.818282341877847
12	3	5.40934829839324
12	4	-0.4824010571201569
12	5	-0.9833369813706891
12	6	3.693277883165525
12	7	14.067568137231063
12	8	14.654393858326447
12	9	14.6938708919046
12	10	15.209247369359726
12	11	13.166937837443456
12	12	12.887817581918704
12	13	8.840239473385537
12	14	10.63005009971302
12	15	7.129297388005254
12	16	11.632114888856464
12	17	7.9170695721257545
12	18	7.773248942069168
12	19	6.570463057541711
12	20	6.582734649869484
12	21	6.62276577978825
12	22	6.039250855262092
12	23	6.397895893120612
12	24	4.20116048121666
12	25	7.052419864779414
12	26	8.461741410898712
12	27	5.2871106733466275
12	28	5.654601391118246
12	29	0.49279309953467276
12	30	6.469493084942525
12	31	8.924551291405225
12	32	8.030302624965547
12	33	8.409860239635517
12	34	9.928024628305527
12	35	8.868807659257097
12	36	2.1000068907372293
12	37	8.36160075879177
12	38	7.205399905961703
12	39	5.993370705449356
12	40	9.552154774064885
14	1	12.86255613924153
14	2	17.65161567521118
14	3	13.90934829839324
14	4	21.85093227621318
14	5	22.183329685295977
14	6	8.526611216498859
14	7	14.567568137231063
14	8	20.48772719165978
14	9	17.52720422523793
14	10	22.875914036026394
14	11	9.333604504110124
14	12	0.054484248585371375
14	13	0.17357280671886954
14	14	-0.03661656695364712
14	15	0.6292973880052539
14	16	10.965448222189796
14	17	8.08373623879242
14	18	9.606582275402502
14	19	10.237129724208376
14	20	8.582734649869483
14	21	6.956099113121584
14	22	9.039250855262093
14	23	2.231229226453946
14	24	3.034493814549994
14	25	19.552419864779413
14	26	8.795074744232046
14	27	21.28711067334663
14	28	22.154601391118245
14	29	11.992793099534673
14	30	19.636159751609192
14	31	17.25788462473856
14	32	24.196969291632215
14	33	21.076526906302185
14	34	24.261357961638858
14	35	15.535474325923765
14	36	19.266673557403895
14	37	25.36160075879177
14	38	11.038733239295038
14	39	10.82670403878269
14	40	19.718821440731553
15	1	9.86255613924153
15	2	3.151615675211179
15	3	4.40934829839324
15	4	10.850932276213177
15	5	-3.316670314704023
15	6	2.526611216498859
15	7	18.567568137231063
15	8	16.98772719165978
15	9	20.52720422523793
15	10	20.875914036026394
15	11	19.333604504110124
15	12	13.054484248585371
15	13	16.67357280671887
15	14	10.463383433046353
15	15	14.629297388005254
15	16	22.965448222189796
15	17	14.58373623879242
15	18	16.606582275402502
15	19	20.737129724208376
15	20	14.582734649869483
15	21	15.956099113121585
15	22	18.539250855262093
15	23	15.231229226453946
15	24	18.034493814549993
15	25	12.052419864779413
15	26	3.7950747442320463
15	27	19.78711067334663
15	28	21.154601391118245
15	29	9.492793099534673
15	30	13.136159751609192
15	31	12.757884624738557
15	32	13.696969291632215
15	33	4.076526906302186
15	34	12.76135796163886
15	35	17.535474325923765
15	36	27.766673557403895
15	37	27.86160075879177
15	38	18.538733239295038
15	39	23.82670403878269
15	40	28.218821440731553
16	1	13.86255613924153
16	2	12.151615675211179
16	3	18.90934829839324
16	4	21.85093227621318
16	5	22.183329685295977
16	6	26.526611216498857
16	7	20.567568137231063
16	8	25.98772719165978
16	9	25.02720422523793
16	10	23.375914036026394
16	11	21.833604504110124
16	12	19.05448424858537
16	13	10.17357280671887
16	14	24.463383433046353
16	15	11.629297388005254
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106	11	-2.266395495889876
106	12	-1.5455157514146283
106	13	-1.2264271932811308
106	14	-4.636616566953647
106	15	0.42929738800525463
106	16	-1.0345517778102042
106	17	2.0837362387924205
106	18	1.0065822754025024
106	19	0.6371297242083775
106	20	0.9827346498694842
106	21	1.5560991131215838
106	22	1.839250855262093
106	23	2.6312292264539465
106	24	1.2344938145499933
106	25	2.3524198647794146
106	26	-3.2049252557679537
106	27	-3.7128893266533725
106	28	-3.845398608881754
106	29	-3.0072069004653272
106	30	-3.363840248390809
106	31	-3.7421153752614433
106	32	-3.8030307083677863
106	33	-3.9234730936978144
106	34	-3.7386420383611405
106	35	-3.464525674076235
106	36	-3.7333264425961046
106	37	-3.63839924120823
106	38	-2.961266760704963
106	39	-3.1732959612173097
106	40	-3.2811785592684473
107	1	7.11255613924153
107	2	6.526615675211179
107	3	9.15934829839324
107	4	-1.149067723786823
107	5	1.933329685295977
107	6	4.151611216498859
107	7	6.9425681372310635
107	8	4.987727191659779
107	9	5.0272042252379325
107	10	-0.6240859639736058
107	11	1.7086045041101237
107	12	3.6794842485853714
107	13	3.0485728067188695
107	14	0.9633834330463529
107	15	5.504297388005254
107	16	-1.2845517778102042
107	17	0.9587362387924205
107	18	-1.518417724597498
107	19	-3.012870275791623
107	20	-3.167265350130516
107	21	-1.6689008868784159
107	22	-1.0857491447379077
107	23	-0.26877077354605383
107	24	-1.965506185450006
107	25	-1.6975801352205862
107	26	-2.7049252557679537
107	27	-3.2128893266533725
107	28	-1.2203986088817542
107	29	-1.2572069004653272
107	30	-1.113840248390809
107	31	-0.8671153752614433
107	32	-0.9280307083677863
107	33	-1.4234730936978144
107	34	-1.1136420383611405
107	35	-0.5895256740762349
107	36	0.14167355740389542
107	37	0.36160075879177
107	38	0.9137332392950368
107	39	0.7017040387826903
107	40	0.5938214407315527
108	1	2.291127567812957
108	2	6.937329960925464
108	3	1.4807768698218116
108	4	1.5652179904988905
108	5	8.183329685295977
108	6	2.669468359356001
108	7	11.281853851516779
108	8	10.630584334516922
108	9	8.95577565380936
108	10	12.447342607454967
108	11	0.33360450411012366
108	12	-4.231230037128914
108	13	-2.8264271932811305
108	14	-2.7509022812393615
108	15	-1.0849883262804596
108	16	0.9654482221897958
108	17	-0.7734066183504362
108	18	1.1780108468310733
108	19	0.8085582956369484
108	20	0.2970203641551983
108	21	2.813241970264442
108	22	4.324965140976379
108	23	1.659800655025375
108	24	0.6059223859785652
108	25	1.6952770076365562
108	26	-0.06206811291081049
108	27	-1.4271750409390869
108	28	2.297458533975388
108	29	3.7070788138203863
108	30	0.35044546589490544
108	31	1.2578846247385567
108	32	0.9112550059179272
108	33	3.362241192016472
108	34	3.404215104496002
108	35	3.2497600402094786
108	36	4.838102128832467
108	37	4.218743615934628
108	38	5.181590382152179
108	39	3.8267040387826903
108	40	4.861678583588695
109	1	1.5292228059081978
109	2	7.540504564100068
109	3	4.687126076171019
109	4	-0.8157343904534891
109	5	5.072218574184866
109	6	6.971055660943302
109	7	9.012012581675506
109	8	7.043282747215336
109	9	6.416093114126822
109	10	2.5425807026930602
109	11	2.4447156152212344
109	12	3.165595359696482
109	13	0.06246169560775883
109	14	2.0744945441574636
109	15	7.4070751657830325
109	16	-1.3678851111435373
109	17	-0.8051526500964687
109	18	-0.1711955023752756
109	19	-1.5406480535694005
109	20	-0.1950431279082938
109	21	-0.9327897757673052
109	22	0.2614730774843146
109	23	-0.10210410687938687
109	24	-0.632172852116673
109	25	-0.5586912463316969
109	26	-1.8715919224346207
109	27	-2.3795559933200394
109	28	-2.956509719992865
109	29	-2.3405402337986603
109	30	-2.2527291372796983
109	31	-3.186559819705888
109	32	-3.247475152812231
109	33	-2.5901397603644813
109	34	-2.62753092725003
109	35	-0.9089701185206795
109	36	-0.8444375537072153
109	37	-0.4161770189860077
109	38	0.2609554615172591
109	39	-2.728851516772865
109	40	-0.05895633704622494
110	1	-7.470777194091804
110	2	-7.348384324788821
110	3	-4.59065170160676
110	4	-2.649067723786823
110	5	-7.816670314704023
110	6	-6.473388783501141
110	7	-8.432431862768937
110	8	-7.512272808340221
110	9	-7.4727957747620675
110	10	-6.124085963973606
110	11	-6.666395495889876
110	12	-5.945515751414629
110	13	-5.8264271932811305
110	14	-6.036616566953647
110	15	-5.370702611994746
110	16	-4.034551777810204
110	17	-3.9162637612075795
110	18	-3.393417724597498
110	19	-3.762870275791623
110	20	-3.417265350130516
110	21	-3.043900886878416
110	22	-2.9607491447379077
110	23	-2.768770773546054
110	24	-2.965506185450006
110	25	-3.447580135220586
110	26	-3.2049252557679537
110	27	-3.7128893266533725
110	28	-3.845398608881754
110	29	-3.0072069004653272
110	30	-3.363840248390809
110	31	-3.7421153752614433
110	32	-3.8030307083677863
110	33	-3.9234730936978144
110	34	-3.7386420383611405
110	35	-3.464525674076235
110	36	-3.7333264425961046
110	37	-3.63839924120823
110	38	-2.961266760704963
110	39	-3.1732959612173097
110	40	-3.2811785592684473
111	1	6.195889472574862
111	2	0.48494900854451295
111	3	-2.2573183682734257
111	4	-1.982401057120157
111	5	0.016663018629310855
111	6	-2.973388783501141
111	7	1.0675681372310635
111	8	1.821060524993113
111	9	1.8605375585712665
111	10	-1.290752630640272
111	11	-5.666395495889876
111	12	-2.1121824180812956
111	13	-0.9930938599477965
111	14	-2.036616566953647
111	15	-2.204035945328079
111	16	-2.201218444476871
111	17	-2.9162637612075795
111	18	-0.3934177245974979
111	19	-1.2628702757916228
111	20	-1.9172653501305161
111	21	-0.3772342202117489
111	22	-0.29408247807124077
111	23	-0.6021041068793869
111	24	-1.965506185450006
111	25	-0.11424680188725311
111	26	-0.8715919224346207
111	27	0.12044400667996058
111	28	-0.8453986088817542
111	29	-2.5072069004653272
111	30	-1.530506915057476
111	31	-1.7421153752614433
111	32	-2.1363640417011194
111	33	-0.5901397603644813
111	34	-1.9053087050278075
111	35	-1.297859007409568
111	36	-0.23332644259610458
111	37	0.86160075879177
111	38	1.8720665726283707
111	39	0.1600373721160233
111	40	1.5521547740648867
112	1	4.86255613924153
112	2	-2.348384324788821
112	3	13.90934829839324
112	4	8.850932276213177
112	5	7.183329685295977
112	6	10.526611216498859
112	7	2.5675681372310635
112	8	-2.512272808340221
112	9	-4.4727957747620675
112	10	-1.1240859639736058
112	11	19.333604504110124
112	12	3.0544842485853714
112	13	16.17357280671887
112	14	8.963383433046353
112	15	15.629297388005254
112	16	6.965448222189796
112	17	21.08373623879242
112	18	5.606582275402502
112	19	7.237129724208377
112	20	6.582734649869484
112	21	2.956099113121584
112	22	13.039250855262093
112	23	12.231229226453946
112	24	16.034493814549993
112	25	19.552419864779413
112	26	14.795074744232046
112	27	12.287110673346628
112	28	19.154601391118245
112	29	14.992793099534673
112	30	14.636159751609192
112	31	25.25788462473856
112	32	27.196969291632215
112	33	27.076526906302185
112	34	26.261357961638858
112	35	7.535474325923765
112	36	25.266673557403895
112	37	-3.63839924120823
112	38	-2.961266760704963
112	39	-3.1732959612173097
112	40	-3.2811785592684473
114	1	13.36255613924153
114	2	-0.848384324788821
114	3	3.4093482983932404
114	4	7.350932276213177
114	5	7.683329685295977
114	6	9.026611216498859
114	7	7.0675681372310635
114	8	7.987727191659779
114	9	8.027204225237933
114	10	-6.124085963973606
114	11	-6.666395495889876
114	12	-5.945515751414629
114	13	-5.8264271932811305
114	14	-6.036616566953647
114	15	-5.370702611994746
114	16	-4.034551777810204
114	17	-3.9162637612075795
114	18	-3.393417724597498
114	19	-3.762870275791623
114	20	-3.417265350130516
114	21	-3.043900886878416
114	22	-2.9607491447379077
114	23	-2.768770773546054
114	24	-2.965506185450006
114	25	-3.447580135220586
114	26	-3.2049252557679537
114	27	-3.7128893266533725
114	28	-3.845398608881754
114	29	-3.0072069004653272
114	30	-3.363840248390809
114	31	-3.7421153752614433
114	32	-3.8030307083677863
114	33	-3.9234730936978144
114	34	-3.7386420383611405
114	35	-3.464525674076235
114	36	-3.7333264425961046
114	37	-3.63839924120823
114	38	-2.961266760704963
114	39	-3.1732959612173097
114	40	-3.2811785592684473
116	1	-7.970777194091804
116	2	-4.015050991455487
116	3	-8.09065170160676
116	4	-7.149067723786823
116	5	-7.816670314704023
116	6	-6.473388783501141
116	7	-8.432431862768937
116	8	-7.512272808340221
116	9	-7.4727957747620675
116	10	-6.124085963973606
116	11	-6.666395495889876
116	12	-5.945515751414629
116	13	-5.8264271932811305
116	14	-6.036616566953647
116	15	-5.370702611994746
116	16	-4.034551777810204
116	17	-3.9162637612075795
116	18	-3.393417724597498
116	19	-3.762870275791623
116	20	-3.417265350130516
116	21	-3.043900886878416
116	22	-2.9607491447379077
116	23	-2.768770773546054
116	24	-2.965506185450006
116	25	-3.447580135220586
116	26	-3.2049252557679537
116	27	-3.7128893266533725
116	28	-3.845398608881754
116	29	-3.0072069004653272
116	30	-3.363840248390809
116	31	-3.7421153752614433
116	32	-3.8030307083677863
116	33	-3.9234730936978144
116	34	-3.7386420383611405
116	35	-3.464525674076235
116	36	-3.7333264425961046
116	37	-3.63839924120823
116	38	-2.961266760704963
116	39	-3.1732959612173097
116	40	-3.2811785592684473
117	1	5.462556139241531
117	2	-1.9483843247888206
117	3	-1.8906517016067603
117	4	0.45093227621317666
117	5	2.7833296852959766
117	6	-1.6733887835011405
117	7	-4.832431862768937
117	8	3.487727191659779
117	9	-2.072795774762067
117	10	-6.124085963973606
117	11	-6.666395495889876
117	12	-5.945515751414629
117	13	-5.8264271932811305
117	14	-6.036616566953647
117	15	-5.370702611994746
117	16	-4.034551777810204
117	17	-3.9162637612075795
117	18	-3.393417724597498
117	19	-3.762870275791623
117	20	-3.417265350130516
117	21	-3.043900886878416
117	22	-2.9607491447379077
117	23	-2.768770773546054
117	24	-2.965506185450006
117	25	-3.447580135220586
117	26	-3.2049252557679537
117	27	-3.7128893266533725
117	28	-3.845398608881754
117	29	-3.0072069004653272
117	30	-3.363840248390809
117	31	-3.7421153752614433
117	32	-3.8030307083677863
117	33	-3.9234730936978144
117	34	-3.7386420383611405
117	35	-3.464525674076235
117	36	-3.7333264425961046
117	37	-3.63839924120823
117	38	-2.961266760704963
117	39	-3.1732959612173097
117	40	-3.2811785592684473
119	1	2.11255613924153
119	2	-4.098384324788821
119	3	3.9093482983932404
119	4	13.350932276213177
119	5	0.43332968529597693
119	6	7.276611216498859
119	7	1.0675681372310635
119	8	6.237727191659779
119	9	-2.2227957747620675
119	10	-0.6240859639736058
119	11	-0.16639549588987634
119	12	0.5544842485853714
119	13	1.4235728067188695
119	14	-6.036616566953647
119	15	-5.370702611994746
119	16	-4.034551777810204
119	17	-3.9162637612075795
119	18	-3.393417724597498
119	19	-3.762870275791623
119	20	-3.417265350130516
119	21	-3.043900886878416
119	22	-2.9607491447379077
119	23	-2.768770773546054
119	24	-2.965506185450006
119	25	-3.447580135220586
119	26	-3.2049252557679537
119	27	-3.7128893266533725
119	28	-3.845398608881754
119	29	-3.0072069004653272
119	30	-3.363840248390809
119	31	-3.7421153752614433
119	32	-3.8030307083677863
119	33	-3.9234730936978144
119	34	-3.7386420383611405
119	35	-3.464525674076235
119	36	-3.7333264425961046
119	37	-3.63839924120823
119	38	-2.961266760704963
119	39	-3.1732959612173097
119	40	-3.2811785592684473
120	1	3.11255613924153
120	2	-5.098384324788821
120	3	-4.09065170160676
120	4	-2.399067723786823
120	5	-0.5666703147040231
120	6	-6.473388783501141
120	7	-8.432431862768937
120	8	-7.512272808340221
120	9	-7.4727957747620675
120	10	-6.124085963973606
120	11	-6.666395495889876
120	12	-5.945515751414629
120	13	-5.8264271932811305
120	14	-6.036616566953647
120	15	-5.370702611994746
120	16	-4.034551777810204
120	17	-3.9162637612075795
120	18	-3.393417724597498
120	19	-3.762870275791623
120	20	-3.417265350130516
120	21	-3.043900886878416
120	22	-2.9607491447379077
120	23	-2.768770773546054
120	24	-2.965506185450006
120	25	-3.447580135220586
120	26	-3.2049252557679537
120	27	-3.7128893266533725
120	28	-3.845398608881754
120	29	-3.0072069004653272
120	30	-3.363840248390809
120	31	-3.7421153752614433
120	32	-3.8030307083677863
120	33	-3.9234730936978144
120	34	-3.7386420383611405
120	35	-3.464525674076235
120	36	-3.7333264425961046
120	37	-3.63839924120823
120	38	-2.961266760704963
120	39	-3.1732959612173097
120	40	-3.2811785592684473
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	2588.0
5	178.0
6	146.0
7	114.0
8	129.0
9	133.0
10	137.0
11	129.0
12	102.0
13	92.0
14	68.0
15	47.0
16	45.0
17	31.0
18	36.0
19	53.0
20	76.0
21	102.0
22	115.0
23	103.0
24	96.0
25	53.0
26	51.0
27	29.0
28	16.0
29	11.0
30	6.0
31	2.0
32	3.0
33	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	98.89078498293516	0.469283276450512	0.44795221843003413	0.19197952218430034
8	96.69156883671292	1.6008537886872998	0.96051227321238	0.7470651013874066
9	92.64078498293516	2.8796928327645053	2.9223549488054608	1.5571672354948805
10	41.98847877106891	27.522935779816514	19.820780883294216	10.667804565820354
11	28.976545842217483	27.67590618336887	19.658848614072493	23.68869936034115
12	30.41160162081467	32.30966090850927	21.28385583280017	15.99488163787588
13	21.262529323949668	26.103646833013432	23.352527191298783	29.28129665173811
14	20.48167092924126	37.89428815004263	25.66069906223359	15.963341858482524
15	35.208866155157715	27.237851662404093	24.126172208013642	13.427109974424553
16	27.088661551577154	36.29582267689685	23.017902813299234	13.597612958226769
17	25.575447570332482	27.195225916453538	33.5464620630861	13.682864450127877
18	24.04092071611253	28.04774083546462	23.891730605285595	24.019607843137255
19	21.568627450980394	30.818414322250636	23.380221653878944	24.232736572890026
20	21.270247229326515	28.72975277067349	36.10400682011935	13.895993179880648
21	19.30946291560102	29.198635976129584	26.51321398124467	24.978687127024724
22	17.71099744245524	25.383631713554987	38.27791986359762	18.627450980392158
23	19.71861010445534	27.691323811554042	36.175655510552126	16.4144105734385
24	22.122762148337596	27.91986359761296	23.99829497016198	25.95907928388747
25	21.643543223052294	29.541088580576307	30.693703308431164	18.121664887940234
26	31.781290046988463	28.919265271251604	21.37975224263135	17.91969243912858
27	19.85451433461703	37.76208814719726	21.908429610611897	20.474967907573813
28	25.378545532096396	36.894860311367026	20.38814246107912	17.338451695457454
29	28.97216274089936	33.190578158458244	22.52676659528908	15.310492505353318
30	26.119402985074625	28.187633262260125	29.12579957356077	16.567164179104477
31	27.152600170502982	34.20716112531969	22.03751065643649	16.602728047740836
32	28.589743589743588	32.17948717948718	23.824786324786324	15.405982905982906
33	26.854219948849106	33.43989769820972	23.891730605285595	15.814151747655583
34	18.712702472293266	29.58226768968457	36.70076726342711	15.004262574595057
35	18.286445012787723	32.11849957374254	35.42199488491048	14.173060528559251
36	22.07264957264957	32.64957264957265	27.564102564102566	17.713675213675213
37	25.916453537936913	30.477408354646208	28.41005967604433	15.196078431372548
38	25.682011935208866	31.47911338448423	26.705029838022163	16.133844842284738
39	23.721227621483376	31.223358908780902	22.37851662404092	22.6768968456948
40	23.06052855924979	29.092071611253196	29.198635976129584	18.648763853367434
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	2.0
8	2.0
9	3.0
10	4.0
11	14.0
12	24.0
13	24.0
14	56.0
15	88.0
16	74.0
17	60.0
18	60.0
19	58.0
20	56.0
21	47.0
22	38.0
23	38.0
24	38.5
25	39.0
26	46.5
27	54.0
28	54.0
29	56.0
30	58.0
31	78.5
32	99.0
33	99.0
34	117.0
35	135.0
36	167.5
37	200.0
38	200.0
39	221.0
40	242.0
41	276.0
42	310.0
43	310.0
44	355.0
45	400.0
46	370.5
47	341.0
48	341.0
49	369.5
50	398.0
51	431.0
52	464.0
53	464.0
54	491.0
55	518.0
56	444.5
57	371.0
58	371.0
59	362.0
60	353.0
61	295.0
62	237.0
63	237.0
64	173.0
65	109.0
66	79.0
67	49.0
68	49.0
69	39.5
70	30.0
71	18.5
72	7.0
73	7.0
74	5.0
75	3.0
76	3.0
77	3.0
78	3.0
79	1.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.08525149190110827
8	0.14919011082693948
9	0.08525149190110827
10	0.10656436487638535
11	0.042625745950554135
12	0.0639386189258312
13	0.0639386189258312
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.021312872975277068
24	0.0
25	0.14919011082693948
26	0.2131287297527707
27	0.3836317135549872
28	0.0639386189258312
29	0.4688832054560955
30	0.042625745950554135
31	0.0
32	0.2557544757033248
33	0.0
34	0.0
35	0.0
36	0.2557544757033248
37	0.0
38	0.0
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	4692.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.6760443307758
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.9088849735716	79.51832907075874
2	4.127863075761389	6.990622335890878
3	0.9061162849232317	2.3017902813299234
4	0.25169896803423103	0.8525149190110828
5	0.17618927762396175	0.7459505541346974
6	0.10067958721369243	0.5115089514066496
7	0.07550969041026932	0.4475703324808184
8	0.10067958721369243	0.6820119352088662
9	0.05033979360684621	0.3836317135549872
>10	0.22652907123080793	3.111679454390452
>50	0.07550969041026932	4.454390451832907
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TAGTCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG	86	1.8329070758738277	No Hit
TAGTCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT	72	1.5345268542199488	No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	51	1.0869565217391304	No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	31	0.6606990622335891	No Hit
TAGTCGGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT	20	0.4262574595055414	No Hit
TAGTCGGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG	20	0.4262574595055414	No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT	17	0.36231884057971014	No Hit
TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15	0.319693094629156	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGTCACAGATCC	12	0.2557544757033248	No Hit
TAGTCGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGA	11	0.23444160272804776	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAA	10	0.2131287297527707	No Hit
TAGTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT	10	0.2131287297527707	No Hit
TAGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	9	0.1918158567774936	No Hit
TAGTCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC	9	0.1918158567774936	No Hit
TAGTCGGGGCAGAGTGCAGTGCTTAGTCGGGGTCACAGAT	8	0.17050298380221654	No Hit
TAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8	0.17050298380221654	No Hit
TAGTCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC	8	0.17050298380221654	No Hit
TAGTCGGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCG	8	0.17050298380221654	No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT	7	0.14919011082693948	No Hit
TAGTCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7	0.14919011082693948	No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC	7	0.14919011082693948	No Hit
TAGTCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG	6	0.1278772378516624	No Hit
TAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6	0.1278772378516624	No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGTCACAGATCCTC	6	0.1278772378516624	No Hit
TAGTCGGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT	6	0.1278772378516624	No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGTCACAGATCCT	5	0.10656436487638535	No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA	5	0.10656436487638535	No Hit
TAGTCGGGGTCACAGATACTCTTCTGAGATGAGTTTTTGT	5	0.10656436487638535	No Hit
TAGTCGGGGACTAATCCTAGCCCTAGCCCTACACAAATAT	5	0.10656436487638535	No Hit
TAGTCGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG	5	0.10656436487638535	No Hit
TAGTCGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAG	5	0.10656436487638535	No Hit
TAGTCGGGGTGTCTGCTTGTCTGTGTATACCACCTGTGTG	5	0.10656436487638535	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.021312872975277068	0.0	0.0	0.0	0.0
15	0.021312872975277068	0.0	0.0	0.0	0.0
16	0.021312872975277068	0.0	0.0	0.0	0.0
17	0.042625745950554135	0.0	0.0	0.0	0.0
18	0.042625745950554135	0.0	0.0	0.0	0.0
19	0.08525149190110827	0.0	0.0	0.0	0.0
20	0.08525149190110827	0.0	0.0	0.0	0.0
21	0.08525149190110827	0.0	0.0	0.0	0.0
22	0.08525149190110827	0.0	0.0	0.0	0.0
23	0.08525149190110827	0.0	0.0	0.0	0.0
24	0.08525149190110827	0.0	0.0	0.0	0.0
25	0.08525149190110827	0.0	0.0	0.0	0.0
26	0.10656436487638533	0.0	0.0	0.0	0.0
27	0.10656436487638533	0.0	0.0	0.0	0.0
28	0.10656436487638533	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGGTA	20	0.0025143002	34.000004	5
CGGGGGA	35	8.1432154E-7	34.000004	5
GTCGGGT	20	0.0025143002	34.000004	3
GGTAAAA	20	0.0025143002	34.000004	8
CGGGGGG	85	0.0	34.0	5
CGGGGGC	30	1.1919767E-5	34.0	5
CGGGGAT	45	3.7271093E-9	34.0	5
CGGGGAG	30	1.1919767E-5	34.0	5
TCGGGGC	45	3.7271093E-9	34.0	4
TCGGGGA	130	0.0	34.0	4
TCGGGGG	175	0.0	33.028572	4
TAGTCGG	480	0.0	32.9375	1
AGTCGGG	475	0.0	32.926315	2
GTCGGGG	440	0.0	32.840908	3
TGAAGGA	55	6.9485395E-10	30.90909	25
CGGGGAA	50	1.0435542E-8	30.599998	5
TCTGAAG	50	1.0435542E-8	30.599998	23
AAGGAGA	50	1.0435542E-8	30.599998	27
TCGGGGT	90	0.0	30.222221	4
GGAGATT	40	2.31396E-6	29.750002	29
>>END_MODULE