SRR1525854_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1525854_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	37132
Sequences flagged as poor quality	0
Sequence length	56
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TCGTAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	227	0.611332543358828	No Hit
TCGTAAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	197	0.5305396962188947	No Hit
TCGTAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	150	0.4039642356996661	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	144	0.3878056662716794	No Hit
TCGTAAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	133	0.3581816223203706	No Hit
TCGTAAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT	103	0.27738877518043736	No Hit
TCGTAAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA	99	0.2666163955617796	No Hit
TCGTAAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC	96	0.2585371108477863	No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	89	0.23968544651513518	No Hit
TCGTAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	85	0.22891306689647745	No Hit
TCGTAAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAAAGCACTGACTGCTCTTTC	84	0.22621997199181298	No Hit
TCGTAAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC	84	0.22621997199181298	No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	71	0.19120973823117526	No Hit
TCGTAAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC	70	0.18851664332651083	No Hit
TCGTAAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGACCTAATACATGCCGAC	68	0.18313045351718193	No Hit
TCGTAAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT	67	0.1804373586125175	No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	65	0.1750511688031886	No Hit
TCGTAAGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG	62	0.1669718840891953	No Hit
TCGTAAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG	61	0.16427878918453087	No Hit
TCGTAAGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC	58	0.15619950447053754	No Hit
TCGTAAGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT	56	0.15081331466120868	No Hit
TCGTAAGGGCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG	54	0.14542712485187978	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC	54	0.14542712485187978	No Hit
TCGTAAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGAC	52	0.14004093504255088	No Hit
TCGTAAGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG	51	0.13734784013788645	No Hit
TCGTAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	49	0.13196165032855758	No Hit
TCGTAAGGGGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAAT	45	0.12118927070989981	No Hit
TCGTAAGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG	44	0.11849617580523537	No Hit
TCGTAAGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTG	43	0.11580308090057094	No Hit
TCGTAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT	42	0.11310998599590649	No Hit
TCGTAAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTCATGGTTCCTTTGGTC	41	0.11041689109124206	No Hit
TCGTAAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC	39	0.10503070128191318	No Hit
TCGTAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	39	0.10503070128191318	No Hit
TCGTAAGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC	38	0.10233760637724874	No Hit
TCGTAAGGGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCG	38	0.10233760637724874	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAATCC	20	3.700764E-4	51.12361	41
CCCCGAC	25	1.7948705E-5	51.12361	41
GCTCATC	15	0.0076617333	51.12361	41
CACGGGC	15	0.0076617333	51.12361	41
TCCAATC	20	3.7777025E-4	50.91148	40
CCCCCGA	20	3.7777025E-4	50.91148	40
GCCCACG	15	0.007831741	50.84116	38
ACCACTG	15	0.007831741	50.84116	39
CGGATTG	35	4.472713E-8	50.84116	39
CCCACGG	15	0.007831741	50.84116	39
GCGGATT	35	4.472713E-8	50.84116	38
TGTCCAA	25	1.8548888E-5	50.841156	38
GTCCAAT	25	1.8548888E-5	50.841156	39
AAAGCAC	15	0.007874678	50.771034	37
TAGAGCA	15	0.008048179	50.49246	35
CATGCCC	15	0.008048179	50.49246	35
TGGGCGG	35	4.723188E-8	50.492455	35
CATCACT	15	0.008135988	50.35431	44
TTGAACA	30	9.715513E-7	50.35431	43
TCATCAC	15	0.008135988	50.35431	43

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content