FastQCFastQC Report
Fri 17 Jun 2016
SRR1525853_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525853_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1113
Sequences flagged as poor quality0
Sequence length40
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAAGGGATGAGATGGACTTAAATGAATTGACGGGTAC90.8086253369272237No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG80.7187780772686434No Hit
TCGTAAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC60.5390835579514826No Hit
TCGTAAGGGATGAGATGGCCTTAAATGAATTGACGGGTAC60.5390835579514826No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG50.44923629829290207No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC50.44923629829290207No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC40.3593890386343217No Hit
TCGTAAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC40.3593890386343217No Hit
TCGTAAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT30.2695417789757413No Hit
TCGTAAGGGCTCGTCTTAATCAGCAACATCTTTTGATACA30.2695417789757413No Hit
TCGTAAGGGGGGGTTGGGGATTTCGCTCAGTGGTAGAGCG30.2695417789757413No Hit
TCGTAAGGGATCCAGAGTCGATTTTTTTTTTTTTTTTTTT30.2695417789757413No Hit
TCGTAAGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT30.2695417789757413No Hit
TCGTAAGGGGGGGTTGGGGATTTCGCTCCGTGGTAGAGCC20.17969451931716085No Hit
TCGTAAGGGAAGTTTAATGTTCTTCCCTGGCACATAGTGG20.17969451931716085No Hit
TCGTAAGGGATGGTCACCCGAGTCGACTTTTTTTTTTTTT20.17969451931716085No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAG20.17969451931716085No Hit
TCGTAAGGGGTGTTCTTTATTAATATCCTAACACTCCTCG20.17969451931716085No Hit
TCGTAAGGGAGTGGTACGCAGAGCGACTTTTTTTTTTTTT20.17969451931716085No Hit
TCGTAAGGGGATCCAGTGACAGATACCATTAGATTCAAGA20.17969451931716085No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTCGAGCG20.17969451931716085No Hit
TCGTAAGGGGTATTGAGTGATGGGCACCTTCCCGGACCTC20.17969451931716085No Hit
TCGTAAGGGGAAAGAGGTGCCTGGTTTACCGACGTGACAG20.17969451931716085No Hit
TCGTAAGGGGGCTTCTTGGGTTGTCTACTTTGTTCCTATT20.17969451931716085No Hit
TCGTAAGGGGTGCCTACCATGGTGACCACGGGTGACGGGG20.17969451931716085No Hit
TCGTAAGGGGTCTTTTCCTCTGGCGCGGTGAGTGCGTTCG20.17969451931716085No Hit
TCGTAAGGGGGGTTTATCCTGTGCGGGGCAAAATGGAGCT20.17969451931716085No Hit
TCGTAAGGGCCTTCTGGGCTGAGGATGCTGGGGCTGCCCG20.17969451931716085No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC20.17969451931716085No Hit
TCGTAAGGGGGGGTTGGGCATTTAGCTCCGTGGTAGAGCG20.17969451931716085No Hit
TCGTAAGGGACCATTTGTGGATGACATGATAGTATGCATA20.17969451931716085No Hit
TCGTAAGGGGGGGGTTGGGGATTTAGCTCCGTGGTCGAGC20.17969451931716085No Hit
TCGTAAGGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.17969451931716085No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGCGCC20.17969451931716085No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGCGCG20.17969451931716085No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCCGTGGTAGACCC20.17969451931716085No Hit
TCGTAAGGGATCCGTCGAGTGACCCTTACTAGTCCTTCTT20.17969451931716085No Hit
TCGTAAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACT20.17969451931716085No Hit
TCGTAAGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTT20.17969451931716085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGGG455.9117156E-1034.0000044
TAAGGGC305.372249E-634.0000044
AAGGGGG200.001748993234.05
CGTAAGG1150.032.521742
TCGTAAG1150.032.521741
GTAAGGG1100.032.4545443
TAAGGGA250.00523709827.24
Produced by FastQC (version 0.11.5)