##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525853_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1113
Sequences flagged as poor quality	0
Sequence length	40
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.47798742138365	29.0	27.0	32.0	4.0	33.0
2	14.088948787061994	11.0	11.0	16.0	4.0	28.0
3	18.461814914645103	19.0	12.0	25.0	4.0	30.0
4	20.762803234501348	24.0	16.0	28.0	4.0	30.0
5	16.761904761904763	17.0	10.0	23.0	4.0	28.0
6	20.168912848158133	22.0	12.0	28.0	4.0	33.0
7	22.955974842767297	27.0	19.0	30.0	4.0	33.0
8	22.00179694519317	26.0	16.0	29.0	4.0	33.0
9	22.155435759209343	26.0	17.0	29.0	4.0	33.0
10	17.22641509433962	18.0	8.0	25.0	4.0	31.0
11	24.20934411500449	29.0	13.0	35.0	4.0	38.0
12	24.22012578616352	29.0	13.0	34.0	4.0	38.0
13	24.716082659478886	29.0	16.0	35.0	4.0	38.0
14	25.13027852650494	30.0	17.0	35.0	4.0	38.0
15	22.92452830188679	27.0	11.0	33.0	4.0	37.0
16	21.11769991015274	25.0	4.0	33.0	4.0	36.0
17	21.863432165318958	26.0	4.0	33.0	4.0	36.0
18	21.29380053908356	26.0	4.0	33.0	4.0	36.0
19	18.073674752920034	18.0	4.0	30.0	4.0	34.0
20	19.990116801437555	23.0	4.0	32.0	4.0	36.0
21	17.4177897574124	15.0	4.0	30.0	4.0	35.0
22	17.98921832884097	19.0	4.0	30.0	4.0	35.0
23	17.78346810422282	18.0	4.0	30.0	4.0	35.0
24	17.519317160826596	17.0	4.0	30.0	4.0	35.0
25	17.00449236298293	15.0	4.0	31.0	4.0	35.0
26	14.03863432165319	4.0	4.0	27.0	4.0	34.0
27	13.752021563342318	4.0	4.0	26.0	4.0	33.0
28	13.324348607367476	4.0	4.0	26.0	4.0	33.0
29	12.771787960467206	4.0	4.0	24.0	4.0	33.0
30	12.2911051212938	4.0	4.0	22.0	4.0	33.0
31	11.629829290206649	4.0	4.0	22.0	4.0	32.0
32	10.978436657681941	4.0	4.0	18.0	4.0	31.0
33	10.78796046720575	4.0	4.0	19.0	4.0	31.0
34	10.778975741239892	4.0	4.0	17.0	4.0	31.0
35	10.215633423180593	4.0	4.0	14.0	4.0	31.0
36	10.382749326145552	4.0	4.0	11.0	4.0	33.0
37	10.171608265947889	4.0	4.0	8.0	4.0	32.0
38	9.940700808625337	4.0	4.0	4.0	4.0	31.0
39	10.133872416891284	4.0	4.0	4.0	4.0	32.0
40	9.790655884995507	4.0	4.0	4.0	4.0	31.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-21.11216216216216
1	2	-9.362612612612612
1	3	-13.347747747747746
1	4	-14.661261261261263
1	5	-11.675225225225226
1	6	-14.795945945945949
1	7	-17.22747747747748
1	8	-16.133783783783784
1	9	-15.70045045045045
1	10	-10.617117117117115
1	11	-16.855855855855854
1	12	-18.154504504504505
1	13	-18.355855855855854
1	14	-18.438288288288287
1	15	-17.402252252252254
1	16	-14.95765765765766
1	17	-14.892342342342342
1	18	-14.451801801801803
1	19	-11.672522522522522
1	20	-13.073873873873875
1	21	-10.914414414414413
1	22	-11.37927927927928
1	23	-11.24099099099099
1	24	-11.949099099099099
1	25	-10.876576576576579
1	26	-8.161711711711712
1	27	-7.237837837837839
1	28	-7.263513513513514
1	29	-7.2779279279279265
1	30	-6.358108108108109
1	31	-6.034234234234233
1	32	-4.8112612612612615
1	33	-5.264414414414414
1	34	-5.000450450450449
1	35	-4.9707207207207205
1	36	-5.0360360360360374
1	37	-5.556306306306308
1	38	-4.612612612612612
1	39	-4.684684684684685
1	40	-4.5472972972972965
5	1	3.8878378378378393
5	2	1.6373873873873883
5	3	-4.347747747747746
5	4	-12.661261261261263
5	5	-2.675225225225226
5	6	-3.795945945945949
5	7	7.772522522522522
5	8	8.866216216216216
5	9	-13.70045045045045
5	10	5.382882882882885
5	11	7.144144144144146
5	12	7.845495495495495
5	13	4.644144144144146
5	14	1.5617117117117125
5	15	3.5977477477477464
5	16	11.04234234234234
5	17	17.107657657657658
5	18	17.548198198198197
5	19	14.327477477477478
5	20	5.926126126126125
5	21	18.085585585585587
5	22	14.62072072072072
5	23	20.75900900900901
5	24	13.050900900900901
5	25	18.12342342342342
5	26	-8.161711711711712
5	27	-7.237837837837839
5	28	-7.263513513513514
5	29	-7.2779279279279265
5	30	-6.358108108108109
5	31	-6.034234234234233
5	32	-4.8112612612612615
5	33	-5.264414414414414
5	34	-5.000450450450449
5	35	-4.9707207207207205
5	36	-5.0360360360360374
5	37	-5.556306306306308
5	38	-4.612612612612612
5	39	-4.684684684684685
5	40	-4.5472972972972965
6	1	4.887837837837839
6	2	0.6373873873873883
6	3	9.652252252252254
6	4	2.338738738738737
6	5	14.324774774774774
6	6	14.204054054054051
6	7	-1.2274774774774784
6	8	-0.13378378378378386
6	9	10.29954954954955
6	10	10.382882882882885
6	11	-10.855855855855854
6	12	4.845495495495495
6	13	-6.355855855855854
6	14	5.5617117117117125
6	15	11.597747747747746
6	16	-5.957657657657659
6	17	12.107657657657658
6	18	7.548198198198197
6	19	16.32747747747748
6	20	-4.073873873873875
6	21	15.085585585585587
6	22	-4.379279279279279
6	23	10.75900900900901
6	24	6.050900900900901
6	25	9.123423423423421
6	26	14.838288288288288
6	27	7.762162162162161
6	28	21.736486486486484
6	29	12.722072072072073
6	30	6.641891891891891
6	31	22.965765765765767
6	32	2.1887387387387385
6	33	23.735585585585586
6	34	0.9995495495495508
6	35	21.02927927927928
6	36	-5.0360360360360374
6	37	-5.556306306306308
6	38	-4.612612612612612
6	39	-4.684684684684685
6	40	-4.5472972972972965
7	1	5.887837837837839
7	2	3.6373873873873883
7	3	5.652252252252254
7	4	9.338738738738737
7	5	15.324774774774774
7	6	-8.795945945945949
7	7	-2.2274774774774784
7	8	-6.133783783783784
7	9	-9.70045045045045
7	10	-0.6171171171171146
7	11	10.144144144144146
7	12	8.845495495495495
7	13	8.644144144144146
7	14	15.561711711711713
7	15	3.5977477477477464
7	16	4.042342342342341
7	17	4.107657657657658
7	18	14.548198198198197
7	19	19.32747747747748
7	20	2.9261261261261247
7	21	-1.9144144144144128
7	22	12.62072072072072
7	23	-4.240990990990991
7	24	16.0509009009009
7	25	19.12342342342342
7	26	5.838288288288288
7	27	-1.237837837837839
7	28	3.736486486486486
7	29	19.722072072072073
7	30	-6.358108108108109
7	31	-6.034234234234233
7	32	-4.8112612612612615
7	33	-5.264414414414414
7	34	-5.000450450450449
7	35	-4.9707207207207205
7	36	-5.0360360360360374
7	37	-5.556306306306308
7	38	-4.612612612612612
7	39	-4.684684684684685
7	40	-4.5472972972972965
9	1	6.554504504504507
9	2	5.637387387387388
9	3	4.985585585585586
9	4	11.67207207207207
9	5	3.324774774774774
9	6	6.870720720720719
9	7	10.43918918918919
9	8	11.866216216216216
9	9	11.632882882882882
9	10	16.382882882882885
9	11	10.477477477477478
9	12	5.512162162162163
9	13	8.310810810810814
9	14	4.895045045045045
9	15	5.264414414414414
9	16	5.375675675675673
9	17	10.44099099099099
9	18	10.214864864864865
9	19	1.9941441441441459
9	20	12.259459459459457
9	21	14.41891891891892
9	22	3.2873873873873887
9	23	16.09234234234234
9	24	13.717567567567569
9	25	17.456756756756757
9	26	-8.161711711711712
9	27	-7.237837837837839
9	28	-7.263513513513514
9	29	-7.2779279279279265
9	30	-6.358108108108109
9	31	-6.034234234234233
9	32	-4.8112612612612615
9	33	-5.264414414414414
9	34	-5.000450450450449
9	35	-4.9707207207207205
9	36	-5.0360360360360374
9	37	-5.556306306306308
9	38	-4.612612612612612
9	39	-4.684684684684685
9	40	-4.5472972972972965
10	1	-4.31216216216216
10	2	-2.3626126126126117
10	3	1.0522522522522522
10	4	1.9387387387387385
10	5	-0.8752252252252255
10	6	1.6040540540540498
10	7	-1.2274774774774784
10	8	0.2662162162162147
10	9	0.2995495495495497
10	10	0.3828828828828854
10	11	-4.855855855855854
10	12	-0.5545045045045036
10	13	-5.355855855855854
10	14	-2.838288288288286
10	15	-1.8022522522522522
10	16	-2.75765765765766
10	17	1.307657657657657
10	18	1.1481981981981981
10	19	-1.0725225225225223
10	20	-1.2738738738738746
10	21	4.085585585585587
10	22	5.020720720720719
10	23	4.759009009009009
10	24	4.4509009009008995
10	25	3.323423423423421
10	26	6.6382882882882885
10	27	10.362162162162162
10	28	5.736486486486486
10	29	10.122072072072072
10	30	5.641891891891891
10	31	6.165765765765766
10	32	6.388738738738738
10	33	6.135585585585586
10	34	1.19954954954955
10	35	-4.9707207207207205
10	36	-5.0360360360360374
10	37	-5.556306306306308
10	38	-4.612612612612612
10	39	-4.684684684684685
10	40	-4.5472972972972965
12	1	2.2211711711711715
12	2	-2.0292792792792778
12	3	2.3189189189189214
12	4	3.672072072072069
12	5	4.658108108108106
12	6	0.2040540540540512
12	7	6.4391891891891895
12	8	6.866216216216216
12	9	4.966216216216218
12	10	7.382882882882885
12	11	10.144144144144146
12	12	8.512162162162163
12	13	11.977477477477482
12	14	6.895045045045045
12	15	6.597747747747746
12	16	7.375675675675673
12	17	11.107657657657658
12	18	11.548198198198197
12	19	9.66081081081081
12	20	14.259459459459457
12	21	17.085585585585587
12	22	16.954054054054055
12	23	11.75900900900901
12	24	17.384234234234235
12	25	20.456756756756757
12	26	8.838288288288288
12	27	11.762162162162161
12	28	13.403153153153154
12	29	7.7220720720720735
12	30	13.641891891891891
12	31	4.632432432432433
12	32	3.8554054054054046
12	33	1.0689189189189197
12	34	2.666216216216217
12	35	3.6959459459459456
12	36	0.2972972972972965
12	37	1.7770270270270263
12	38	4.387387387387388
12	39	1.9819819819819813
12	40	0.7860360360360374
13	1	6.387837837837839
13	2	5.137387387387388
13	3	-9.347747747747746
13	4	-11.661261261261263
13	5	-9.175225225225226
13	6	-9.795945945945949
13	7	-11.227477477477478
13	8	-8.133783783783784
13	9	-10.20045045045045
13	10	-5.617117117117115
13	11	-3.3558558558558538
13	12	-4.154504504504505
13	13	-4.355855855855854
13	14	-7.9382882882882875
13	15	-9.402252252252254
13	16	-2.457657657657659
13	17	-1.3923423423423422
13	18	-14.451801801801803
13	19	-11.672522522522522
13	20	-13.073873873873875
13	21	-10.914414414414413
13	22	-11.37927927927928
13	23	-11.24099099099099
13	24	-11.949099099099099
13	25	-10.876576576576579
13	26	-8.161711711711712
13	27	-7.237837837837839
13	28	-7.263513513513514
13	29	-7.2779279279279265
13	30	-6.358108108108109
13	31	-6.034234234234233
13	32	-4.8112612612612615
13	33	-5.264414414414414
13	34	-5.000450450450449
13	35	-4.9707207207207205
13	36	-5.0360360360360374
13	37	-5.556306306306308
13	38	-4.612612612612612
13	39	-4.684684684684685
13	40	-4.5472972972972965
15	1	5.887837837837839
15	2	7.137387387387388
15	3	8.652252252252254
15	4	3.338738738738737
15	5	7.824774774774774
15	6	4.704054054054051
15	7	11.772522522522522
15	8	12.366216216216216
15	9	11.79954954954955
15	10	11.382882882882885
15	11	13.144144144144146
15	12	13.345495495495495
15	13	12.144144144144146
15	14	3.0617117117117125
15	15	11.597747747747746
15	16	6.542342342342341
15	17	6.107657657657658
15	18	14.048198198198197
15	19	11.827477477477478
15	20	7.426126126126125
15	21	16.585585585585587
15	22	4.120720720720721
15	23	12.75900900900901
15	24	12.550900900900901
15	25	12.123423423423421
15	26	21.33828828828829
15	27	15.262162162162161
15	28	17.236486486486484
15	29	19.722072072072073
15	30	20.14189189189189
15	31	9.465765765765767
15	32	10.688738738738738
15	33	0.23558558558558573
15	34	10.49954954954955
15	35	10.52927927927928
15	36	8.463963963963963
15	37	7.943693693693692
15	38	8.887387387387388
15	39	-0.1846846846846848
15	40	8.952702702702704
16	1	7.887837837837839
16	2	9.304054054054056
16	3	10.652252252252254
16	4	11.338738738738737
16	5	10.991441441441442
16	6	10.204054054054051
16	7	9.772522522522522
16	8	11.532882882882884
16	9	11.29954954954955
16	10	16.049549549549553
16	11	17.477477477477482
16	12	16.17882882882883
16	13	14.31081081081081
16	14	14.895045045045048
16	15	14.597747747747746
16	16	16.709009009009005
16	17	15.440990990990993
16	18	15.881531531531532
16	19	14.66081081081081
16	20	18.926126126126125
16	21	10.752252252252255
16	22	16.954054054054055
16	23	12.75900900900901
16	24	18.384234234234235
16	25	9.456756756756754
16	26	-8.161711711711712
16	27	-7.237837837837839
16	28	-7.263513513513514
16	29	-7.2779279279279265
16	30	-6.358108108108109
16	31	-6.034234234234233
16	32	-4.8112612612612615
16	33	-5.264414414414414
16	34	-5.000450450450449
16	35	-4.9707207207207205
16	36	-5.0360360360360374
16	37	-5.556306306306308
16	38	-4.612612612612612
16	39	-4.684684684684685
16	40	-4.5472972972972965
17	1	7.887837837837839
17	2	19.63738738738739
17	3	8.152252252252254
17	4	9.338738738738737
17	5	2.324774774774774
17	6	2.704054054054051
17	7	10.772522522522522
17	8	8.866216216216216
17	9	10.79954954954955
17	10	4.382882882882885
17	11	10.144144144144146
17	12	2.845495495495495
17	13	13.144144144144146
17	14	9.561711711711713
17	15	7.597747747747746
17	16	8.04234234234234
17	17	4.607657657657658
17	18	12.548198198198197
17	19	12.327477477477478
17	20	9.426126126126125
17	21	7.585585585585587
17	22	11.62072072072072
17	23	11.25900900900901
17	24	11.550900900900901
17	25	6.6234234234234215
17	26	-4.161711711711712
17	27	6.262162162162161
17	28	12.236486486486486
17	29	5.7220720720720735
17	30	15.641891891891891
17	31	-0.5342342342342334
17	32	0.6887387387387385
17	33	11.235585585585586
17	34	17.99954954954955
17	35	10.52927927927928
17	36	25.463963963963963
17	37	19.943693693693692
17	38	23.38738738738739
17	39	25.315315315315317
17	40	21.4527027027027
18	1	6.554504504504507
18	2	0.3040540540540544
18	3	0.9855855855855857
18	4	6.005405405405405
18	5	3.324774774774774
18	6	0.5373873873873833
18	7	7.772522522522522
18	8	4.199549549549548
18	9	6.966216216216218
18	10	6.049549549549553
18	11	-1.8558558558558538
18	12	0.845495495495495
18	13	0.3108108108108141
18	14	1.8950450450450447
18	15	3.9310810810810786
18	16	-1.2909909909909913
18	17	11.107657657657658
18	18	7.214864864864865
18	19	11.66081081081081
18	20	9.592792792792793
18	21	-1.2477477477477468
18	22	1.954054054054053
18	23	-1.2409909909909906
18	24	5.050900900900901
18	25	-1.8765765765765785
18	26	7.838288288288288
18	27	6.428828828828829
18	28	6.403153153153154
18	29	11.388738738738741
18	30	10.641891891891891
18	31	2.6324324324324326
18	32	4.522072072072072
18	33	7.402252252252254
18	34	12.666216216216219
18	35	3.0292792792792795
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107	37	24.943693693693692
107	38	18.38738738738739
107	39	25.315315315315317
107	40	6.9527027027027035
109	1	3.8878378378378393
109	2	-0.3626126126126117
109	3	-1.3477477477477464
109	4	1.338738738738737
109	5	-2.675225225225226
109	6	-2.795945945945949
109	7	7.772522522522522
109	8	6.866216216216216
109	9	4.29954954954955
109	10	-0.6171171171171146
109	11	3.1441441441441462
109	12	10.845495495495495
109	13	8.644144144144146
109	14	6.5617117117117125
109	15	-4.402252252252254
109	16	13.04234234234234
109	17	14.107657657657658
109	18	11.548198198198197
109	19	16.32747747747748
109	20	14.926126126126125
109	21	-0.9144144144144128
109	22	10.62072072072072
109	23	-0.24099099099099064
109	24	7.050900900900901
109	25	-2.8765765765765785
109	26	-3.161711711711712
109	27	5.762162162162161
109	28	1.736486486486486
109	29	4.7220720720720735
109	30	-0.3581081081081088
109	31	4.965765765765767
109	32	7.1887387387387385
109	33	0.7355855855855857
109	34	14.99954954954955
109	35	20.02927927927928
109	36	24.963963963963963
109	37	20.443693693693692
109	38	25.38738738738739
109	39	14.315315315315315
109	40	20.4527027027027
110	1	7.887837837837839
110	2	-3.3626126126126117
110	3	12.652252252252254
110	4	3.338738738738737
110	5	12.324774774774774
110	6	1.2040540540540512
110	7	2.7725225225225216
110	8	2.866216216216216
110	9	7.29954954954955
110	10	-3.6171171171171146
110	11	-10.855855855855854
110	12	8.845495495495495
110	13	5.644144144144146
110	14	1.5617117117117125
110	15	1.5977477477477464
110	16	14.04234234234234
110	17	14.107657657657658
110	18	14.548198198198197
110	19	-2.672522522522522
110	20	9.926126126126125
110	21	18.085585585585587
110	22	16.62072072072072
110	23	10.75900900900901
110	24	18.0509009009009
110	25	11.123423423423421
110	26	21.83828828828829
110	27	17.762162162162163
110	28	21.736486486486484
110	29	17.722072072072073
110	30	23.64189189189189
110	31	-6.034234234234233
110	32	-4.8112612612612615
110	33	-5.264414414414414
110	34	-5.000450450450449
110	35	-4.9707207207207205
110	36	-5.0360360360360374
110	37	-5.556306306306308
110	38	-4.612612612612612
110	39	-4.684684684684685
110	40	-4.5472972972972965
112	1	8.88783783783784
112	2	12.637387387387388
112	3	15.652252252252254
112	4	8.338738738738737
112	5	16.324774774774774
112	6	6.204054054054051
112	7	12.772522522522522
112	8	6.866216216216216
112	9	12.29954954954955
112	10	-10.617117117117115
112	11	-16.855855855855854
112	12	-18.154504504504505
112	13	-18.355855855855854
112	14	-18.438288288288287
112	15	-17.402252252252254
112	16	-14.95765765765766
112	17	-14.892342342342342
112	18	-14.451801801801803
112	19	-11.672522522522522
112	20	-13.073873873873875
112	21	-10.914414414414413
112	22	-11.37927927927928
112	23	-11.24099099099099
112	24	-11.949099099099099
112	25	-10.876576576576579
112	26	-8.161711711711712
112	27	-7.237837837837839
112	28	-7.263513513513514
112	29	-7.2779279279279265
112	30	-6.358108108108109
112	31	-6.034234234234233
112	32	-4.8112612612612615
112	33	-5.264414414414414
112	34	-5.000450450450449
112	35	-4.9707207207207205
112	36	-5.0360360360360374
112	37	-5.556306306306308
112	38	-4.612612612612612
112	39	-4.684684684684685
112	40	-4.5472972972972965
114	1	-0.11216216216216068
114	2	-2.3626126126126117
114	3	3.6522522522522536
114	4	4.338738738738737
114	5	3.324774774774774
114	6	1.2040540540540512
114	7	-5.227477477477478
114	8	8.866216216216216
114	9	-12.70045045045045
114	10	-1.6171171171171146
114	11	-10.855855855855854
114	12	10.845495495495495
114	13	2.6441441441441462
114	14	-12.438288288288287
114	15	11.597747747747746
114	16	11.04234234234234
114	17	-14.892342342342342
114	18	-14.451801801801803
114	19	-11.672522522522522
114	20	-13.073873873873875
114	21	-10.914414414414413
114	22	-11.37927927927928
114	23	-11.24099099099099
114	24	-11.949099099099099
114	25	-10.876576576576579
114	26	-8.161711711711712
114	27	-7.237837837837839
114	28	-7.263513513513514
114	29	-7.2779279279279265
114	30	-6.358108108108109
114	31	-6.034234234234233
114	32	-4.8112612612612615
114	33	-5.264414414414414
114	34	-5.000450450450449
114	35	-4.9707207207207205
114	36	-5.0360360360360374
114	37	-5.556306306306308
114	38	-4.612612612612612
114	39	-4.684684684684685
114	40	-4.5472972972972965
118	1	-21.11216216216216
118	2	-9.362612612612612
118	3	-13.347747747747746
118	4	-14.661261261261263
118	5	-11.675225225225226
118	6	-14.795945945945949
118	7	-17.22747747747748
118	8	-16.133783783783784
118	9	-15.70045045045045
118	10	-10.617117117117115
118	11	-16.855855855855854
118	12	-18.154504504504505
118	13	-18.355855855855854
118	14	-18.438288288288287
118	15	-17.402252252252254
118	16	-14.95765765765766
118	17	-14.892342342342342
118	18	-14.451801801801803
118	19	-11.672522522522522
118	20	-13.073873873873875
118	21	-10.914414414414413
118	22	-11.37927927927928
118	23	-11.24099099099099
118	24	-11.949099099099099
118	25	-10.876576576576579
118	26	-8.161711711711712
118	27	-7.237837837837839
118	28	-7.263513513513514
118	29	-7.2779279279279265
118	30	-6.358108108108109
118	31	-6.034234234234233
118	32	-4.8112612612612615
118	33	-5.264414414414414
118	34	-5.000450450450449
118	35	-4.9707207207207205
118	36	-5.0360360360360374
118	37	-5.556306306306308
118	38	-4.612612612612612
118	39	-4.684684684684685
118	40	-4.5472972972972965
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	196.0
5	12.0
6	2.0
7	3.0
8	5.0
9	11.0
10	22.0
11	38.0
12	42.0
13	38.0
14	33.0
15	46.0
16	47.0
17	49.0
18	54.0
19	53.0
20	51.0
21	57.0
22	49.0
23	54.0
24	59.0
25	45.0
26	48.0
27	28.0
28	27.0
29	16.0
30	12.0
31	10.0
32	2.0
33	2.0
34	1.0
35	0.0
36	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.45994599459947	0.54005400540054	0.0	0.0
8	98.92183288409704	0.44923629829290207	0.2695417789757413	0.3593890386343217
9	99.64061096136568	0.2695417789757413	0.08984725965858043	0.0
10	54.98652291105122	27.942497753818508	7.008086253369273	10.062893081761008
11	39.17340521114106	24.079065588499553	21.83288409703504	14.914645103324348
12	44.74393530997305	14.465408805031446	19.137466307277627	21.653189577717878
13	29.919137466307276	22.282120395327944	23.180592991913745	24.61814914645103
14	23.98921832884097	21.47349505840072	33.24348607367475	21.293800539083556
15	19.047619047619047	26.864330637915547	35.669362084456424	18.418688230008982
16	31.98562443845463	18.059299191374663	30.00898472596586	19.946091644204852
17	38.36477987421384	18.508535489667565	24.348607367475292	18.778077268643305
18	37.64600179694519	19.946091644204852	20.664869721473494	21.743036837376458
19	24.797114517583406	17.583408476104598	19.47700631199279	38.14247069431921
20	26.774483378256964	24.079065588499553	23.71967654986523	25.426774483378257
21	20.30548068283917	20.664869721473494	35.669362084456424	23.360287511230908
22	19.85624438454627	17.4303683737646	35.130278526504945	27.583108715184185
23	25.067385444743934	19.766397124887693	32.34501347708895	22.821203953279426
24	21.563342318059302	25.516621743036836	25.78616352201258	27.133872416891286
25	28.39173405211141	22.731356693620842	22.192273135669364	26.68463611859838
26	17.944093778178537	18.575293056807933	28.22362488728584	35.25698827772768
27	20.934411500449237	14.016172506738545	32.61455525606469	32.43486073674753
28	16.366906474820144	21.223021582733814	27.248201438848923	35.16187050359712
29	16.831683168316832	20.7020702070207	32.04320432043205	30.423042304230425
30	22.41224122412241	11.071107110711072	41.494149414941496	25.02250225022502
31	15.945945945945947	14.054054054054054	45.22522522522522	24.774774774774773
32	33.692722371967655	10.512129380053908	32.70440251572327	23.090745732255165
33	25.899280575539567	14.658273381294965	36.8705035971223	22.571942446043167
34	15.992812219227314	12.848158131176998	44.56424079065589	26.5947888589398
35	19.946091644204852	17.969451931716083	39.17340521114106	22.911051212938006
36	24.887690925426774	15.184186882300091	36.38814016172507	23.539982030548067
37	21.962196219621962	18.09180918091809	34.02340234023402	25.922592259225922
38	19.58670260557053	17.969451931716083	38.72416891284816	23.71967654986523
39	18.508535489667565	18.149146451033243	34.052111410601974	29.290206648697215
40	18.957771787960468	15.902964959568733	34.681042228212036	30.45822102425876
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	2.0
16	1.5
17	1.0
18	1.0
19	6.5
20	12.0
21	10.0
22	8.0
23	8.0
24	10.5
25	13.0
26	16.0
27	19.0
28	19.0
29	19.5
30	20.0
31	23.5
32	27.0
33	27.0
34	30.5
35	34.0
36	35.5
37	37.0
38	37.0
39	47.5
40	58.0
41	60.0
42	62.0
43	62.0
44	64.5
45	67.0
46	66.5
47	66.0
48	66.0
49	71.5
50	77.0
51	73.5
52	70.0
53	70.0
54	68.5
55	67.0
56	81.0
57	95.0
58	95.0
59	99.5
60	104.0
61	93.5
62	83.0
63	83.0
64	84.0
65	85.0
66	69.0
67	53.0
68	53.0
69	38.0
70	23.0
71	19.0
72	15.0
73	15.0
74	11.5
75	8.0
76	5.5
77	3.0
78	3.0
79	3.0
80	3.0
81	2.0
82	1.0
83	1.0
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.17969451931716085
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.3593890386343217
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.3593890386343217
27	0.0
28	0.08984725965858043
29	0.17969451931716085
30	0.17969451931716085
31	0.2695417789757413
32	0.0
33	0.08984725965858043
34	0.0
35	0.0
36	0.0
37	0.17969451931716085
38	0.0
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	1113.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.26145552560648
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.24277456647398	89.75741239892183
2	2.5048169556840074	4.672057502246182
3	0.48169556840077066	1.3477088948787064
4	0.1926782273603083	0.7187780772686434
5	0.1926782273603083	0.8984725965858041
6	0.1926782273603083	1.078167115902965
7	0.0	0.0
8	0.09633911368015415	0.7187780772686434
9	0.09633911368015415	0.8086253369272237
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGTAAGGGATGAGATGGACTTAAATGAATTGACGGGTAC	9	0.8086253369272237	No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG	8	0.7187780772686434	No Hit
TCGTAAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC	6	0.5390835579514826	No Hit
TCGTAAGGGATGAGATGGCCTTAAATGAATTGACGGGTAC	6	0.5390835579514826	No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG	5	0.44923629829290207	No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC	5	0.44923629829290207	No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC	4	0.3593890386343217	No Hit
TCGTAAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC	4	0.3593890386343217	No Hit
TCGTAAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT	3	0.2695417789757413	No Hit
TCGTAAGGGCTCGTCTTAATCAGCAACATCTTTTGATACA	3	0.2695417789757413	No Hit
TCGTAAGGGGGGGTTGGGGATTTCGCTCAGTGGTAGAGCG	3	0.2695417789757413	No Hit
TCGTAAGGGATCCAGAGTCGATTTTTTTTTTTTTTTTTTT	3	0.2695417789757413	No Hit
TCGTAAGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.2695417789757413	No Hit
TCGTAAGGGGGGGTTGGGGATTTCGCTCCGTGGTAGAGCC	2	0.17969451931716085	No Hit
TCGTAAGGGAAGTTTAATGTTCTTCCCTGGCACATAGTGG	2	0.17969451931716085	No Hit
TCGTAAGGGATGGTCACCCGAGTCGACTTTTTTTTTTTTT	2	0.17969451931716085	No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAG	2	0.17969451931716085	No Hit
TCGTAAGGGGTGTTCTTTATTAATATCCTAACACTCCTCG	2	0.17969451931716085	No Hit
TCGTAAGGGAGTGGTACGCAGAGCGACTTTTTTTTTTTTT	2	0.17969451931716085	No Hit
TCGTAAGGGGATCCAGTGACAGATACCATTAGATTCAAGA	2	0.17969451931716085	No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTCGAGCG	2	0.17969451931716085	No Hit
TCGTAAGGGGTATTGAGTGATGGGCACCTTCCCGGACCTC	2	0.17969451931716085	No Hit
TCGTAAGGGGAAAGAGGTGCCTGGTTTACCGACGTGACAG	2	0.17969451931716085	No Hit
TCGTAAGGGGGCTTCTTGGGTTGTCTACTTTGTTCCTATT	2	0.17969451931716085	No Hit
TCGTAAGGGGTGCCTACCATGGTGACCACGGGTGACGGGG	2	0.17969451931716085	No Hit
TCGTAAGGGGTCTTTTCCTCTGGCGCGGTGAGTGCGTTCG	2	0.17969451931716085	No Hit
TCGTAAGGGGGGTTTATCCTGTGCGGGGCAAAATGGAGCT	2	0.17969451931716085	No Hit
TCGTAAGGGCCTTCTGGGCTGAGGATGCTGGGGCTGCCCG	2	0.17969451931716085	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	2	0.17969451931716085	No Hit
TCGTAAGGGGGGGTTGGGCATTTAGCTCCGTGGTAGAGCG	2	0.17969451931716085	No Hit
TCGTAAGGGACCATTTGTGGATGACATGATAGTATGCATA	2	0.17969451931716085	No Hit
TCGTAAGGGGGGGGTTGGGGATTTAGCTCCGTGGTCGAGC	2	0.17969451931716085	No Hit
TCGTAAGGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.17969451931716085	No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGCGCC	2	0.17969451931716085	No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGCGCG	2	0.17969451931716085	No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCCGTGGTAGACCC	2	0.17969451931716085	No Hit
TCGTAAGGGATCCGTCGAGTGACCCTTACTAGTCCTTCTT	2	0.17969451931716085	No Hit
TCGTAAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACT	2	0.17969451931716085	No Hit
TCGTAAGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTT	2	0.17969451931716085	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGGGG	45	5.9117156E-10	34.000004	4
TAAGGGC	30	5.372249E-6	34.000004	4
AAGGGGG	20	0.0017489932	34.0	5
CGTAAGG	115	0.0	32.52174	2
TCGTAAG	115	0.0	32.52174	1
GTAAGGG	110	0.0	32.454544	3
TAAGGGA	25	0.005237098	27.2	4
>>END_MODULE