FastQCFastQC Report
Fri 17 Jun 2016
SRR1525852_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525852_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119
Sequences flagged as poor quality0
Sequence length40
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAAGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21.680672268907563No Hit
TCGTAAGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTTT21.680672268907563No Hit
TCGTAAGGGATATCAAAGTTGCTCCAGTTAAAAAGCTCGT10.8403361344537815No Hit
TCGTAAGGGAGAGTCCCACCCCAGCGCCTTTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGGGTACAGGAAGAGGAGGTGGAGGAAGAGGCG10.8403361344537815No Hit
TCGTAAGGGCCCATCAGCTTTATACACTGTTCCTTGTGAG10.8403361344537815No Hit
TCGTAAGGGAGTGCAGTGCTTCGTAAGGGGTACCCTCGGT10.8403361344537815No Hit
TCGTAAGGGCCGTGTTCCCCACCGCCCCCTTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGGCTTCCCTCCCCCCCCCCCCCCCCTCGGCGC10.8403361344537815No Hit
TCGTAAGGGATTTTCAATTTTCCCAACCATTTTCATTCCG10.8403361344537815No Hit
TCGTAAGGGAAGCATGGTCTCAACCCAAAAAAAAAAAAAA10.8403361344537815No Hit
TCGTAAGGGGGGGACTTCGTGGAGAACGCAATGCCTCCCC10.8403361344537815No Hit
TCGTAAGGGGTTCAGGAAATGATAATTNNCGCCCATTCCA10.8403361344537815No Hit
TCGTAAGGGGTTGTTGTTGTTTTGGTTTGGTTTGTTCTGT10.8403361344537815No Hit
TCGTAAGGGTGCCGAGTCATTTTTTTTTTTTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGGAGAGCACACACCCATCCGTGCACCACTGCA10.8403361344537815No Hit
TCGTAAGGGAGGATTTGGCACTTAAAGCCCAGTGCTTTCG10.8403361344537815No Hit
TCGTAAGGGGGGAATTCGTGGCGAACGAAATGCCTCCCCC10.8403361344537815No Hit
TCGTAAGGGGGTGGGTTCTGTGAGGTGTCCAAGAACCTGG10.8403361344537815No Hit
TCGTAAGGGGTGCAGTGCTTCGTAAGGGCCCAGCGTCGCC10.8403361344537815No Hit
TCGTAAGGGAGTCACGCAGAGTCGCCTTTTTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGGGTGCCGACTTTTTTTTTTTTTTTTTTTTTT10.8403361344537815No Hit
TCGTAACAGTGATCCAAGTAACTGTGCCAGTGTCAGTAAT10.8403361344537815No Hit
TCGTAAGGGACGCTGGTACCACCCCAGCGCCGACCTTTTT10.8403361344537815No Hit
TCGTAAGGGTCCCCCATACAACTTATCCTTACTTTTTCTT10.8403361344537815No Hit
TCGTAAGGGAATAATAAATGCGTTTGGCTGGGTGTAGGGG10.8403361344537815No Hit
TCGTAAGGGGCTGAAGGACTAACACAGCAAATTAAATGGG10.8403361344537815No Hit
TCGTAAGGGGTGCCGTGCCCCGTACGGGCGCCTGGTCACC10.8403361344537815No Hit
TCGTAAGGGCAGAGTGACCCGCGAGCCCCCTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTCTT10.8403361344537815No Hit
TCGTAAGGGCCCCCTGGCCCTGATGGGCCCTGGGACATGT10.8403361344537815No Hit
TCGTAAGGGGCCGAATACCCTCCCCCCCCACCCGCCCGGA10.8403361344537815No Hit
TCGTAAGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTCC10.8403361344537815No Hit
TCGTAAGGGGGTGCTTCGCAAGGGGGCGTTGGTTTTGCAC10.8403361344537815No Hit
TCGTAAGGGGGTACAGGAAGAGGAGGTGGAGGAAGAGGAG10.8403361344537815No Hit
TCGTAAGGGGCATTTTTTTTAGTGCCCGCCTTGCCCTATC10.8403361344537815No Hit
TCGTAAGGGCCTCGTTTTTTTTTTTTTTTTTTTTTTCCCC10.8403361344537815No Hit
TCGTAAGGGATGCTTTTGTTCTCATTTTCTTCCTCGTAAT10.8403361344537815No Hit
TCGTAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGACCTTAACCCCGAGCCCACTTTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTCT10.8403361344537815No Hit
TCGTAAGGGGGGCTGGTGCGATGGCTCCGTGGGTACGAGC10.8403361344537815No Hit
TCGTAAGGGGGGCAAGACCTTTGCTAGTTAGAAGCTGGCC10.8403361344537815No Hit
TCGTAAATTAGACCAAAACAATCTCCCCTTTATCAACTAC10.8403361344537815No Hit
TCGTAAGGGAAGCTGGTCCACCCCGCGCCGACTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGTGGTAGATCGATTTTTTTTTTTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGACGCAGTGGCATAAACCCTTTGAACCCTTGA10.8403361344537815No Hit
TCGTAAGGGCCTTTTTCCTTTCCCTCCTGCGCCCCCAGCC10.8403361344537815No Hit
TCGTAAGGGGCGTCGACTTTTTTTTTTTTTTTTTTTCCTC10.8403361344537815No Hit
TCGTAAGGGTGGCACCCAGCCCCACTTTTTTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGACGACGGACCCAGCCCACTTTTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGAAGCATGGTTCAACCCCAGCCGATTTTTTTT10.8403361344537815No Hit
TCGTAAGGGCTTTGCAAATGCTTTGTACCCAGCGTGATAC10.8403361344537815No Hit
TCGTAAGGGAACCATGGAATCAACCCAGAGCCGCCTTTTT10.8403361344537815No Hit
TCGTAAGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTTC10.8403361344537815No Hit
TCGTAAGGCAGACATCAAAATGAATCCCCAACCAAAACTC10.8403361344537815No Hit
TCGTAAGGGACGCACTCATACCCACCCCACCTCTTTTTTC10.8403361344537815No Hit
TCGTAAGTGATGAGATGGGATGTACCATGGAGCCCTCACT10.8403361344537815No Hit
TCGTAAGGGAGGTTTTCCTGTCAAGTTCCGGCCCTTCTTC10.8403361344537815No Hit
TCGTAAGTGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGATGGACCCACCCAGAGCCCACCGGAAGACCC10.8403361344537815No Hit
TCGTAAGGGGGGCAACCACCGAGCGGGCCCCCTTGACACC10.8403361344537815No Hit
TCGTAAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGAACATGTATCAACCCCGCGCCGACTTTTTTT10.8403361344537815No Hit
TCGTAAGGGAGTGGATTTTTTTTTTTTTTTTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGGTGGTTTTTTGTTTTGTTTTGTTTTTTTTTG10.8403361344537815No Hit
TCGTAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGACTTTTTTTTCTTTTTTTTTTTTTTTTTTTC10.8403361344537815No Hit
TCGTAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAACAAAA10.8403361344537815No Hit
TCGTAAGGGTTCGCCTTTTTTTTTTTTTTTTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGGGTTTTCCCCTTCTCCCTTCCCCGGCGGTCC10.8403361344537815No Hit
TCGTAAGGGGCTAAGTCCTGTTTTACGACGCCCGCCACCA10.8403361344537815No Hit
TCGTAAGGGACTTTTTTTTTTTTTCTTTAATTTTTTTTAT10.8403361344537815No Hit
TCGTAAGGGACGATTTTCTCTCGCCAAAAATACATATGCT10.8403361344537815No Hit
TCGTAAGGGAGCACGAACCATTTTTTTTTTTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGCCGCATGTTCCACCCAAGCCGCCTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGTCGCCTTTTTCCCCCCCACCCCACCCTCACC10.8403361344537815No Hit
TCGTAAGGGCTGTTTCATCAAGTCCTCTGACTTGCCTCCC10.8403361344537815No Hit
TCGTAAGGGATTCCAGGCCGTTTTTTTTTTTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGACGCCGTATCAACCCAGAGTCGGTTTGTTTG10.8403361344537815No Hit
TCGTAAGGGACCTGGTACAACGCAGAGTCGGCTCGGAAGA10.8403361344537815No Hit
TCGTAAGGGAAGCAGTGGCATCATTTTTTTTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGGACAGACCCCCCCCCCACGGCGATTGTGGTA10.8403361344537815No Hit
TCGTAAGGGTGGTTCCCCCCAGAGTTTTTTTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGAGATGGCCCCCAACCATTTTTTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGATGGTTCAACCCAGAGCCGACTCTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGCCTACACGCCGATCCGCTTTTTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGCTGGATCAACCCAGAGCCGCCTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGACCCAGCGCCGCTTTTTTTTTTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGGCTTTTTTTTTCTTCCAAATGTCCCCTGTCC10.8403361344537815No Hit
TCGTAAGGGCTCCTCCTAACCACCAACACCTTTTGATCAA10.8403361344537815No Hit
TCGTAAGGGCCCGAACCCTTTTTTTTTTTCTAGACTCACC10.8403361344537815No Hit
TCGTAAGGGGGTTTTCCCCTTATCCCTCCACCGGACCTAC10.8403361344537815No Hit
TCGTAAGGGGTTTCCTCTTGGCCAACGTTCGTGTCCACGT10.8403361344537815No Hit
TCGTAAGACACGCTAAACCAACAAATCTAGTCACACACCC10.8403361344537815No Hit
TCGTAAGGGACCTCGTATCAACCCAGCGCCGCCCTTTGTT10.8403361344537815No Hit
TCGTAAGGGGGTTTCAAGGCTGGCCGCCGTCCCGTGGCCT10.8403361344537815No Hit
TCGTAANGGACTTTTTTTTTTTTTTTTTTTTTTTTTGGAA10.8403361344537815No Hit
TCGTAAGGGATCGTACCCTGAATACATACGGCGGTATTCC10.8403361344537815No Hit
TCGTAAGGGGGGTTATGCCTTTTTTTAAAGTACCTTTTCG10.8403361344537815No Hit
TCGTAACCCTCCCAACCCTTCACCCTACGTCTTGAGAGTC10.8403361344537815No Hit
TCGTAAGGGCGTTCCAACCCACCCCTTCTTTCTTTCNTTT10.8403361344537815No Hit
TCGTAAGGGAAGCATGGTCTCAACGAAAAAAAAAACGACC10.8403361344537815No Hit
TCGTAAGGGACTGGTCACAGAGCGCTTTTTTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGCGCTGGTCAACCGAGCCGCCTTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGCCCCACCCCCCCTTTTTTTTTTTTTTTTTTT10.8403361344537815No Hit
TCGTAAGGGAAGCCTAGACACCGATCGGTACGTTTCTAGA10.8403361344537815No Hit
TCGTAAGGGAGCACATGTCCATCGGACCACTTTAAGGCGT10.8403361344537815No Hit
TCGTAAGGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC10.8403361344537815No Hit
TCGTAAGGGGGGGCTGGAGAGATGGCTCACCCGTTAACAG10.8403361344537815No Hit
TCGTAAGGGACTATAGTTACTGTTCGAGGTCCTTTCATTC10.8403361344537815No Hit
TCGTAAGGGGGGCCTGGTGAGATGGCCCAGTGGGTCAGAG10.8403361344537815No Hit
TCGTAAGGGAGCGTGGTCAACCCAGAGTCAACCTTTTTTT10.8403361344537815No Hit
TCGTAAGGGGGTCTTCCCATCCCCCTCCACGACGCCAGAC10.8403361344537815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGG100.034.03
CGTAAGG100.034.02
TCGTAAG100.034.01