FastQCFastQC Report
Fri 17 Jun 2016
SRR1525851_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525851_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1448
Sequences flagged as poor quality0
Sequence length40
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC151.0359116022099446No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG50.3453038674033149No Hit
TCGTAAGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT50.3453038674033149No Hit
TCGTAAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.2762430939226519No Hit
TCGTAAGGGGGGGAGGTAGTGACGAAAAATAACAATACAG40.2762430939226519No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG40.2762430939226519No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA40.2762430939226519No Hit
TCGTAAGGGGTGTTCTTTATTAATATCCTAACACTCCTCG40.2762430939226519No Hit
TCGTAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT40.2762430939226519No Hit
TCGTAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT40.2762430939226519No Hit
TCGTAAGGGATAGCAGGATTTGTTCACTGATTCCCATTAT30.20718232044198895No Hit
TCGTAAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACT30.20718232044198895No Hit
TCGTAAGGGACTAATCCTAGCCCTAGCCCTACACAAATAT30.20718232044198895No Hit
TCGTAAGGGCGGGGAGGTAGTGACGAAAAATAACAATACA30.20718232044198895No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGA30.20718232044198895No Hit
TCGTAAGGGGTCTTTCCTGCCCTCTGTCGTCTGTTTTAAG30.20718232044198895No Hit
TCGTAAGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGC20.13812154696132595No Hit
TCGTAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG20.13812154696132595No Hit
TCGTAAGGGGTTGCTTACATTTTACTGTACATTTTTCTGA20.13812154696132595No Hit
TCGTAAGGGGATTCCGGAGAGGGAGCCTGAGAAACGGCTA20.13812154696132595No Hit
TCGTAAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT20.13812154696132595No Hit
TCGTAAGGGGGGAGACAACTACTGCCAAATGAATAAACTC20.13812154696132595No Hit
TCGTAAGGGTCACAGACCCTCTTCTGAGATGAGTTTTTGT20.13812154696132595No Hit
TCGTAAGGGCGTAGGAGTAAACATAACATTCTTCCCTCAA20.13812154696132595No Hit
TCGTAAGGGGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA20.13812154696132595No Hit
TCGTAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG20.13812154696132595No Hit
TCGTAAGGGGATGTGCAGCTAGGCATTAAGGACCCCTCTG20.13812154696132595No Hit
TCGTAAGGGGACCATGCTGTGTTAGGGGATGTGTTCCAGG20.13812154696132595No Hit
TCGTAAGNGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC20.13812154696132595No Hit
TCGTAAAGGCAAACCTTTATTTATAAGTGTGATGCAGTTC20.13812154696132595No Hit
TCGTAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.13812154696132595No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC20.13812154696132595No Hit
TCGTAAGGGGTAAATCATTAATCTTCTGACAAGAAGCTGA20.13812154696132595No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAAAG20.13812154696132595No Hit
TCGTAAGGGATGCCACAACTAGATACATCAACATGATTTA20.13812154696132595No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGGTGGGTAAGA20.13812154696132595No Hit
TCGTAAGGGGGCAGATCCTGGCACTCACTGACACCAGTCT20.13812154696132595No Hit
TCGTAAGGGTGTTTGTTTTTCCTTAGAATGGATTTTGGTT20.13812154696132595No Hit
TCGTAAGGGGTCTAAGTACGCACGGCCGGTACAGTGAAAC20.13812154696132595No Hit
TCGTAAGGGTGTTCTTTATTAATATCCTAACACTCCTCGT20.13812154696132595No Hit
TCGTAAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAG20.13812154696132595No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAAC20.13812154696132595No Hit
TCGTAAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCAC20.13812154696132595No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTTAGTGGGTAAGAG20.13812154696132595No Hit
TCGTAAGGGATTCGTAGGAGTAAACATAACATTCTTCCCT20.13812154696132595No Hit
TCGTAAGGGGGGTCAGGAGTTTCACCCATTGCTTCTTCAA20.13812154696132595No Hit
TCGTAAGGGGGGTTTCTATCATCCTGTCTCTGTTTAAAAG20.13812154696132595No Hit
TCGTAAGGGGGAGCTATTTTAATATGTTGTATAAAGCGAA20.13812154696132595No Hit
TCGTAAGGGGTTAGGGGGCAGATAAAGTGGTCAGGCTGAG20.13812154696132595No Hit
TCGTAAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA20.13812154696132595No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG251.1732582E-434.06
AAGGGGG306.8233385E-634.05
CGTAAGG1250.034.02
TCGTAAG1300.034.01
TAAGGGG650.034.04
TAAGGGA251.1732582E-434.04
AAGGGGT200.001945374933.9999965
TAAGGGT200.001945374933.9999964
GTAAGGG1250.032.643
Produced by FastQC (version 0.11.5)