##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525851_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1448 Sequences flagged as poor quality 0 Sequence length 40 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 14.091850828729282 4.0 4.0 29.0 4.0 32.0 2 9.339779005524862 4.0 4.0 15.0 4.0 26.0 3 9.115331491712707 4.0 4.0 13.0 4.0 24.0 4 8.58011049723757 4.0 4.0 12.0 4.0 22.0 5 8.4792817679558 4.0 4.0 11.0 4.0 22.0 6 8.5103591160221 4.0 4.0 11.0 4.0 23.0 7 9.17403314917127 4.0 4.0 11.0 4.0 26.0 8 9.17817679558011 4.0 4.0 11.0 4.0 26.0 9 8.962707182320441 4.0 4.0 10.0 4.0 25.0 10 8.41988950276243 4.0 4.0 9.0 4.0 23.0 11 9.6146408839779 4.0 4.0 7.0 4.0 31.0 12 9.484806629834255 4.0 4.0 6.0 4.0 31.0 13 8.887430939226519 4.0 4.0 4.0 4.0 27.0 14 9.103591160220995 4.0 4.0 4.0 4.0 29.0 15 9.191988950276244 4.0 4.0 4.0 4.0 30.0 16 8.069060773480663 4.0 4.0 4.0 4.0 26.0 17 7.61671270718232 4.0 4.0 4.0 4.0 23.0 18 7.599447513812155 4.0 4.0 4.0 4.0 23.0 19 7.25207182320442 4.0 4.0 4.0 4.0 22.0 20 7.3252762430939224 4.0 4.0 4.0 4.0 23.0 21 6.584254143646409 4.0 4.0 4.0 4.0 17.0 22 6.346685082872928 4.0 4.0 4.0 4.0 15.0 23 6.486878453038674 4.0 4.0 4.0 4.0 15.0 24 6.3687845303867405 4.0 4.0 4.0 4.0 16.0 25 6.363259668508287 4.0 4.0 4.0 4.0 15.0 26 6.5628453038674035 4.0 4.0 4.0 4.0 17.0 27 6.553176795580111 4.0 4.0 4.0 4.0 17.0 28 6.61671270718232 4.0 4.0 4.0 4.0 17.0 29 6.348066298342541 4.0 4.0 4.0 4.0 14.0 30 6.430939226519337 4.0 4.0 4.0 4.0 14.0 31 6.714088397790055 4.0 4.0 4.0 4.0 18.0 32 6.648480662983426 4.0 4.0 4.0 4.0 19.0 33 6.611187845303867 4.0 4.0 4.0 4.0 17.0 34 6.665745856353591 4.0 4.0 4.0 4.0 18.0 35 6.662983425414365 4.0 4.0 4.0 4.0 18.0 36 6.647790055248619 4.0 4.0 4.0 4.0 16.0 37 6.703729281767956 4.0 4.0 4.0 4.0 17.0 38 6.599447513812155 4.0 4.0 4.0 4.0 14.0 39 6.598756906077348 4.0 4.0 4.0 4.0 14.0 40 6.616022099447513 4.0 4.0 4.0 4.0 15.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 18.796019900497512 1 2 13.365671641791042 1 3 4.643034825870645 1 4 19.263681592039802 1 5 23.48258706467662 1 6 8.747512437810945 1 7 7.648009950248758 1 8 -5.057213930348258 1 9 -5.541044776119405 1 10 -4.618159203980099 1 11 -6.890547263681592 1 12 -5.337064676616913 1 13 -4.493781094527364 1 14 -5.231343283582092 1 15 -5.368159203980099 1 16 -3.912935323383084 1 17 -3.0111940298507474 1 18 -3.449004975124379 1 19 -2.9340796019900504 1 20 -2.9253731343283578 1 21 -2.618159203980099 1 22 -2.4154228855721396 1 23 -2.4726368159203984 1 24 -2.215174129353233 1 25 -2.278606965174129 1 26 -2.5360696517412933 1 27 -2.3743781094527368 1 28 -2.884328358208955 1 29 -2.544776119402985 1 30 -2.487562189054727 1 31 -3.1753731343283578 1 32 -2.8631840796019894 1 33 -3.0597014925373136 1 34 -2.7935323383084567 1 35 -2.772388059701494 1 36 -3.034825870646765 1 37 -3.0024875621890548 1 38 -2.646766169154229 1 39 -2.8345771144278604 1 40 -2.880597014925372 2 1 2.7960199004975106 2 2 7.365671641791042 2 3 -5.356965174129355 2 4 -5.2363184079602 2 5 -5.517412935323382 2 6 -4.752487562189055 2 7 -5.851990049751242 2 8 -5.057213930348258 2 9 -5.541044776119405 2 10 -4.618159203980099 2 11 -6.890547263681592 2 12 -5.337064676616913 2 13 -4.493781094527364 2 14 -5.231343283582092 2 15 -5.368159203980099 2 16 -3.912935323383084 2 17 -3.0111940298507474 2 18 -3.449004975124379 2 19 -2.9340796019900504 2 20 -2.9253731343283578 2 21 -2.618159203980099 2 22 -2.4154228855721396 2 23 -2.4726368159203984 2 24 -2.215174129353233 2 25 -2.278606965174129 2 26 -2.5360696517412933 2 27 -2.3743781094527368 2 28 -2.884328358208955 2 29 -2.544776119402985 2 30 -2.487562189054727 2 31 -3.1753731343283578 2 32 -2.8631840796019894 2 33 -3.0597014925373136 2 34 -2.7935323383084567 2 35 -2.772388059701494 2 36 -3.034825870646765 2 37 -3.0024875621890548 2 38 -2.646766169154229 2 39 -2.8345771144278604 2 40 -2.880597014925372 3 1 -10.20398009950249 3 2 -5.634328358208958 3 3 -5.356965174129355 3 4 -5.2363184079602 3 5 -5.517412935323382 3 6 -4.752487562189055 3 7 -5.851990049751242 3 8 -5.057213930348258 3 9 -5.541044776119405 3 10 -4.618159203980099 3 11 -6.890547263681592 3 12 -5.337064676616913 3 13 -4.493781094527364 3 14 -5.231343283582092 3 15 -5.368159203980099 3 16 -3.912935323383084 3 17 -3.0111940298507474 3 18 -3.449004975124379 3 19 -2.9340796019900504 3 20 -2.9253731343283578 3 21 -2.618159203980099 3 22 -2.4154228855721396 3 23 -2.4726368159203984 3 24 -2.215174129353233 3 25 -2.278606965174129 3 26 -2.5360696517412933 3 27 -2.3743781094527368 3 28 -2.884328358208955 3 29 -2.544776119402985 3 30 -2.487562189054727 3 31 -3.1753731343283578 3 32 -2.8631840796019894 3 33 -3.0597014925373136 3 34 -2.7935323383084567 3 35 -2.772388059701494 3 36 -3.034825870646765 3 37 -3.0024875621890548 3 38 -2.646766169154229 3 39 -2.8345771144278604 3 40 -2.880597014925372 6 1 17.796019900497512 6 2 10.365671641791042 6 3 21.643034825870643 6 4 16.763681592039802 6 5 1.4825870646766184 6 6 17.247512437810947 6 7 20.148009950248756 6 8 23.942786069651742 6 9 23.458955223880594 6 10 25.3818407960199 6 11 19.10945273631841 6 12 11.662935323383087 6 13 1.506218905472636 6 14 11.768656716417908 6 15 23.6318407960199 6 16 -3.912935323383084 6 17 -3.0111940298507474 6 18 -3.449004975124379 6 19 -2.9340796019900504 6 20 -2.9253731343283578 6 21 -2.618159203980099 6 22 -2.4154228855721396 6 23 -2.4726368159203984 6 24 -2.215174129353233 6 25 -2.278606965174129 6 26 -2.5360696517412933 6 27 -2.3743781094527368 6 28 -2.884328358208955 6 29 -2.544776119402985 6 30 -2.487562189054727 6 31 -3.1753731343283578 6 32 -2.8631840796019894 6 33 -3.0597014925373136 6 34 -2.7935323383084567 6 35 -2.772388059701494 6 36 -3.034825870646765 6 37 -3.0024875621890548 6 38 -2.646766169154229 6 39 -2.8345771144278604 6 40 -2.880597014925372 8 1 3.2960199004975106 8 2 -0.6343283582089576 8 3 -3.356965174129355 8 4 0.01368159203980035 8 5 7.482587064676618 8 6 8.247512437810945 8 7 -3.851990049751242 8 8 -2.557213930348258 8 9 -3.0410447761194046 8 10 -2.368159203980099 8 11 -6.140547263681592 8 12 -2.3370646766169134 8 13 -2.743781094527364 8 14 -4.481343283582092 8 15 -2.618159203980099 8 16 2.587064676616916 8 17 3.9888059701492526 8 18 3.050995024875621 8 19 3.5659203980099496 8 20 3.0746268656716422 8 21 2.631840796019901 8 22 2.8345771144278604 8 23 1.5273631840796016 8 24 0.03482587064676679 8 25 -0.028606965174128973 8 26 1.4639303482587067 8 27 1.6256218905472632 8 28 1.115671641791045 8 29 -0.794776119402985 8 30 0.512437810945273 8 31 3.0746268656716422 8 32 3.8868159203980106 8 33 4.440298507462686 8 34 3.4564676616915433 8 35 3.477611940298506 8 36 1.965174129353235 8 37 3.9975124378109452 8 38 4.353233830845771 8 39 4.16542288557214 8 40 4.119402985074628 9 1 15.462686567164178 9 2 15.03233830845771 9 3 17.643034825870643 9 4 15.7636815920398 9 5 -0.1840796019900477 9 6 0.24751243781094523 9 7 17.481343283582092 9 8 4.942786069651742 9 9 14.125621890547263 9 10 6.048507462686567 9 11 12.44278606965174 9 12 9.662935323383087 9 13 12.172885572139304 9 14 2.101990049751242 9 15 7.298507462686569 9 16 5.42039800995025 9 17 1.3221393034825866 9 18 13.21766169154229 9 19 5.06592039800995 9 20 6.741293532338308 9 21 14.715174129353233 9 22 8.917910447761194 9 23 7.194029850746268 9 24 18.1181592039801 9 25 10.388059701492539 9 26 16.79726368159204 9 27 12.625621890547263 9 28 12.782338308457714 9 29 21.121890547263682 9 30 21.17910447761194 9 31 19.157960199004975 9 32 23.470149253731343 9 33 14.273631840796018 9 34 21.206467661691544 9 35 18.894278606965173 9 36 27.6318407960199 9 37 25.997512437810947 9 38 22.35323383084577 9 39 17.49875621890547 9 40 17.45273631840796 10 1 15.79601990049751 10 2 -3.6343283582089576 10 3 11.643034825870645 10 4 6.7636815920398 10 5 6.482587064676618 10 6 5.247512437810945 10 7 16.148009950248756 10 8 11.942786069651742 10 9 16.458955223880594 10 10 14.381840796019901 10 11 12.109452736318408 10 12 5.662935323383087 10 13 18.506218905472636 10 14 13.768656716417908 10 15 23.6318407960199 10 16 25.087064676616915 10 17 19.98880597014925 10 18 21.550995024875622 10 19 24.06592039800995 10 20 24.074626865671643 10 21 24.3818407960199 10 22 16.58457711442786 10 23 24.5273631840796 10 24 24.784825870646767 10 25 24.721393034825873 10 26 28.463930348258707 10 27 28.625621890547265 10 28 28.115671641791046 10 29 28.455223880597014 10 30 28.512437810945272 10 31 27.824626865671643 10 32 28.13681592039801 10 33 27.940298507462686 10 34 17.206467661691544 10 35 28.227611940298505 10 36 27.965174129353237 10 37 27.997512437810947 10 38 28.35323383084577 10 39 28.16542288557214 10 40 28.11940298507463 13 1 -10.20398009950249 13 2 -5.634328358208958 13 3 -5.356965174129355 13 4 -5.2363184079602 13 5 -5.517412935323382 13 6 -4.752487562189055 13 7 -5.851990049751242 13 8 -5.057213930348258 13 9 -5.541044776119405 13 10 -4.618159203980099 13 11 -6.890547263681592 13 12 -5.337064676616913 13 13 -4.493781094527364 13 14 -5.231343283582092 13 15 -5.368159203980099 13 16 -3.912935323383084 13 17 -3.0111940298507474 13 18 -3.449004975124379 13 19 -2.9340796019900504 13 20 -2.9253731343283578 13 21 -2.618159203980099 13 22 -2.4154228855721396 13 23 -2.4726368159203984 13 24 -2.215174129353233 13 25 -2.278606965174129 13 26 -2.5360696517412933 13 27 -2.3743781094527368 13 28 -2.884328358208955 13 29 -2.544776119402985 13 30 -2.487562189054727 13 31 -3.1753731343283578 13 32 -2.8631840796019894 13 33 -3.0597014925373136 13 34 -2.7935323383084567 13 35 -2.772388059701494 13 36 -3.034825870646765 13 37 -3.0024875621890548 13 38 -2.646766169154229 13 39 -2.8345771144278604 13 40 -2.880597014925372 16 1 15.29601990049751 16 2 15.365671641791042 16 3 8.643034825870645 16 4 9.2636815920398 16 5 4.482587064676618 16 6 14.914179104477613 16 7 11.814676616915426 16 8 18.942786069651742 16 9 16.458955223880594 16 10 15.715174129353233 16 11 16.94278606965174 16 12 18.829601990049753 16 13 17.006218905472636 16 14 15.935323383084576 16 15 16.965174129353233 16 16 21.587064676616915 16 17 20.155472636815922 16 18 20.71766169154229 16 19 14.399253731343283 16 20 20.74129353233831 16 21 18.54850746268657 16 22 14.917910447761193 16 23 16.69402985074627 16 24 10.284825870646767 16 25 19.0547263681592 16 26 17.79726368159204 16 27 19.79228855721393 16 28 10.615671641791046 16 29 3.121890547263681 16 30 7.012437810945273 16 31 5.824626865671642 16 32 9.63681592039801 16 33 10.606965174129353 16 34 8.373134328358208 16 35 9.894278606965173 16 36 9.465174129353235 16 37 5.830845771144279 16 38 6.519900497512437 16 39 4.4987562189054735 16 40 3.952736318407962 18 1 6.796019900497511 18 2 -1.6343283582089576 18 3 3.976368159203979 18 4 4.430348258706466 18 5 5.815920398009952 18 6 6.580845771144279 18 7 7.814676616915426 18 8 7.942786069651742 18 9 6.458955223880595 18 10 3.381840796019901 18 11 13.776119402985076 18 12 11.329601990049754 18 13 7.506218905472636 18 14 15.10199004975124 18 15 15.631840796019901 18 16 5.753731343283582 18 17 6.655472636815919 18 18 4.217661691542287 18 19 6.732587064676616 18 20 6.741293532338308 18 21 0.7151741293532341 18 22 3.5845771144278604 18 23 6.527363184079602 18 24 5.118159203980101 18 25 1.054726368159204 18 26 2.130597014925373 18 27 3.6256218905472632 18 28 2.449004975124379 18 29 1.788557213930349 18 30 0.512437810945273 18 31 6.491293532338308 18 32 6.803482587064677 18 33 4.940298507462686 18 34 4.206467661691543 18 35 1.2276119402985062 18 36 6.631840796019901 18 37 2.6641791044776113 18 38 7.353233830845771 18 39 6.4987562189054735 18 40 6.786069651741294 19 1 10.04601990049751 19 2 7.615671641791042 19 3 6.643034825870645 19 4 7.5136815920398 19 5 6.232587064676618 19 6 9.747512437810945 19 7 13.898009950248758 19 8 13.692786069651742 19 9 10.208955223880595 19 10 13.881840796019901 19 11 12.609452736318408 19 12 12.912935323383087 19 13 8.756218905472636 19 14 14.518656716417908 19 15 15.881840796019901 19 16 9.337064676616915 19 17 10.238805970149253 19 18 6.800995024875621 19 19 11.31592039800995 19 20 4.324626865671642 19 21 2.881840796019901 19 22 2.0845771144278604 19 23 2.5273631840796016 19 24 1.5348258706467668 19 25 2.471393034825871 19 26 1.4639303482587067 19 27 1.1256218905472632 19 28 2.865671641791045 19 29 1.455223880597015 19 30 0.012437810945272965 19 31 3.5746268656716422 19 32 1.1368159203980106 19 33 2.1902985074626864 19 34 -0.2935323383084567 19 35 1.9776119402985062 19 36 4.215174129353235 19 37 2.2475124378109452 19 38 3.353233830845771 19 39 3.6654228855721396 19 40 4.369402985074628 20 1 -3.7039800995024894 20 2 -5.634328358208958 20 3 -5.356965174129355 20 4 -5.2363184079602 20 5 -5.517412935323382 20 6 -4.752487562189055 20 7 -5.851990049751242 20 8 -5.057213930348258 20 9 -5.541044776119405 20 10 -4.618159203980099 20 11 -6.890547263681592 20 12 -5.337064676616913 20 13 -4.493781094527364 20 14 -5.231343283582092 20 15 -5.368159203980099 20 16 -3.912935323383084 20 17 -3.0111940298507474 20 18 -3.449004975124379 20 19 -2.9340796019900504 20 20 -2.9253731343283578 20 21 -2.618159203980099 20 22 -2.4154228855721396 20 23 -2.4726368159203984 20 24 -2.215174129353233 20 25 -2.278606965174129 20 26 -2.5360696517412933 20 27 -2.3743781094527368 20 28 -2.884328358208955 20 29 -2.544776119402985 20 30 -2.487562189054727 20 31 -3.1753731343283578 20 32 -2.8631840796019894 20 33 -3.0597014925373136 20 34 -2.7935323383084567 20 35 -2.772388059701494 20 36 -3.034825870646765 20 37 -3.0024875621890548 20 38 -2.646766169154229 20 39 -2.8345771144278604 20 40 -2.880597014925372 21 1 13.79601990049751 21 2 8.365671641791042 21 3 18.643034825870643 21 4 21.763681592039802 21 5 -5.517412935323382 21 6 -4.752487562189055 21 7 -5.851990049751242 21 8 -5.057213930348258 21 9 -5.541044776119405 21 10 -4.618159203980099 21 11 -6.890547263681592 21 12 -5.337064676616913 21 13 -4.493781094527364 21 14 -5.231343283582092 21 15 -5.368159203980099 21 16 -3.912935323383084 21 17 -3.0111940298507474 21 18 -3.449004975124379 21 19 -2.9340796019900504 21 20 -2.9253731343283578 21 21 -2.618159203980099 21 22 -2.4154228855721396 21 23 -2.4726368159203984 21 24 -2.215174129353233 21 25 -2.278606965174129 21 26 -2.5360696517412933 21 27 -2.3743781094527368 21 28 -2.884328358208955 21 29 -2.544776119402985 21 30 -2.487562189054727 21 31 -3.1753731343283578 21 32 -2.8631840796019894 21 33 -3.0597014925373136 21 34 -2.7935323383084567 21 35 -2.772388059701494 21 36 -3.034825870646765 21 37 -3.0024875621890548 21 38 -2.646766169154229 21 39 -2.8345771144278604 21 40 -2.880597014925372 26 1 14.79601990049751 26 2 5.365671641791042 26 3 1.6430348258706449 26 4 -1.2363184079601997 26 5 19.48258706467662 26 6 7.247512437810945 26 7 14.148009950248758 26 8 16.942786069651742 26 9 13.458955223880595 26 10 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2.4402985074626864 107 34 3.9564676616915433 107 35 3.977611940298506 107 36 5.215174129353235 107 37 0.24751243781094523 107 38 5.853233830845771 107 39 2.9154228855721396 107 40 5.369402985074628 109 1 2.2960199004975106 109 2 3.3656716417910424 109 3 2.643034825870645 109 4 -1.2363184079601997 109 5 -3.5174129353233816 109 6 -1.7524875621890548 109 7 5.148009950248758 109 8 7.442786069651742 109 9 5.958955223880595 109 10 1.381840796019901 109 11 8.609452736318408 109 12 6.662935323383087 109 13 3.506218905472636 109 14 7.768656716417908 109 15 10.131840796019901 109 16 -3.912935323383084 109 17 -3.0111940298507474 109 18 -3.449004975124379 109 19 -2.9340796019900504 109 20 -2.9253731343283578 109 21 -2.618159203980099 109 22 -2.4154228855721396 109 23 -2.4726368159203984 109 24 -2.215174129353233 109 25 -2.278606965174129 109 26 -2.5360696517412933 109 27 -2.3743781094527368 109 28 -2.884328358208955 109 29 -2.544776119402985 109 30 -2.487562189054727 109 31 -3.1753731343283578 109 32 -2.8631840796019894 109 33 -3.0597014925373136 109 34 -2.7935323383084567 109 35 -2.772388059701494 109 36 -3.034825870646765 109 37 -3.0024875621890548 109 38 -2.646766169154229 109 39 -2.8345771144278604 109 40 -2.880597014925372 111 1 -10.20398009950249 111 2 -5.634328358208958 111 3 -5.356965174129355 111 4 -5.2363184079602 111 5 -5.517412935323382 111 6 -4.752487562189055 111 7 -5.851990049751242 111 8 -5.057213930348258 111 9 -5.541044776119405 111 10 -4.618159203980099 111 11 -6.890547263681592 111 12 -5.337064676616913 111 13 -4.493781094527364 111 14 -5.231343283582092 111 15 -5.368159203980099 111 16 -3.912935323383084 111 17 -3.0111940298507474 111 18 -3.449004975124379 111 19 -2.9340796019900504 111 20 -2.9253731343283578 111 21 -2.618159203980099 111 22 -2.4154228855721396 111 23 -2.4726368159203984 111 24 -2.215174129353233 111 25 -2.278606965174129 111 26 -2.5360696517412933 111 27 -2.3743781094527368 111 28 -2.884328358208955 111 29 -2.544776119402985 111 30 -2.487562189054727 111 31 -3.1753731343283578 111 32 -2.8631840796019894 111 33 -3.0597014925373136 111 34 -2.7935323383084567 111 35 -2.772388059701494 111 36 -3.034825870646765 111 37 -3.0024875621890548 111 38 -2.646766169154229 111 39 -2.8345771144278604 111 40 -2.880597014925372 114 1 -10.20398009950249 114 2 -5.634328358208958 114 3 -5.356965174129355 114 4 -5.2363184079602 114 5 -5.517412935323382 114 6 -4.752487562189055 114 7 -5.851990049751242 114 8 -5.057213930348258 114 9 -5.541044776119405 114 10 -4.618159203980099 114 11 -6.890547263681592 114 12 -5.337064676616913 114 13 -4.493781094527364 114 14 -5.231343283582092 114 15 -5.368159203980099 114 16 -3.912935323383084 114 17 -3.0111940298507474 114 18 -3.449004975124379 114 19 -2.9340796019900504 114 20 -2.9253731343283578 114 21 -2.618159203980099 114 22 -2.4154228855721396 114 23 -2.4726368159203984 114 24 -2.215174129353233 114 25 -2.278606965174129 114 26 -2.5360696517412933 114 27 -2.3743781094527368 114 28 -2.884328358208955 114 29 -2.544776119402985 114 30 -2.487562189054727 114 31 -3.1753731343283578 114 32 -2.8631840796019894 114 33 -3.0597014925373136 114 34 -2.7935323383084567 114 35 -2.772388059701494 114 36 -3.034825870646765 114 37 -3.0024875621890548 114 38 -2.646766169154229 114 39 -2.8345771144278604 114 40 -2.880597014925372 116 1 -1.8706467661691555 116 2 -2.3009950248756246 116 3 -4.023631840796022 116 4 -0.9029850746268657 116 5 1.1492537313432845 116 6 -1.0858208955223878 116 7 -0.851990049751242 116 8 0.942786069651742 116 9 -0.20771144278607068 116 10 -3.951492537313432 116 11 -3.890547263681592 116 12 -2.6703980099502465 116 13 -0.16044776119403004 116 14 -2.231343283582092 116 15 -2.368159203980099 116 16 3.753731343283582 116 17 -2.3445273631840804 116 18 2.550995024875621 116 19 -1.2674129353233834 116 20 -1.5920398009950247 116 21 -1.284825870646766 116 22 3.5845771144278604 116 23 0.19402985074626855 116 24 3.784825870646767 116 25 0.721393034825871 116 26 3.4639303482587067 116 27 5.625621890547263 116 28 5.115671641791045 116 29 5.455223880597015 116 30 5.512437810945273 116 31 7.824626865671642 116 32 7.4701492537313445 116 33 7.940298507462686 116 34 6.539800995024877 116 35 8.894278606965173 116 36 7.298507462686569 116 37 8.33084577114428 116 38 8.686567164179106 116 39 8.498756218905474 116 40 6.452736318407962 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 986.0 5 20.0 6 40.0 7 13.0 8 11.0 9 23.0 10 28.0 11 38.0 12 31.0 13 29.0 14 19.0 15 12.0 16 14.0 17 10.0 18 5.0 19 8.0 20 20.0 21 24.0 22 30.0 23 27.0 24 25.0 25 12.0 26 13.0 27 5.0 28 3.0 29 1.0 30 0.0 31 0.0 32 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 98.96337249481687 0.48375950241879756 0.3455425017277125 0.2073255010366275 8 98.19694868238558 0.5547850208044383 0.9015256588072121 0.34674063800277394 9 97.50865051903114 1.107266435986159 1.314878892733564 0.06920415224913494 10 61.74033149171271 20.441988950276244 8.08011049723757 9.737569060773481 11 38.950276243093924 24.171270718232044 20.78729281767956 16.091160220994475 12 33.97790055248619 21.408839779005525 25.276243093922652 19.337016574585636 13 27.900552486187845 22.030386740331494 30.0414364640884 20.027624309392262 14 23.756906077348066 26.312154696132595 28.7292817679558 21.201657458563535 15 27.624309392265197 26.243093922651934 31.146408839779006 14.986187845303867 16 32.59668508287293 27.486187845303867 24.378453038674035 15.538674033149173 17 28.541810642709052 25.362819626814098 30.06219765031099 16.033172080165862 18 29.005524861878452 25.75966850828729 29.55801104972376 15.676795580110497 19 24.187975120939875 30.20041465100207 26.74498963372495 18.866620594333103 20 29.074585635359117 25.0 30.662983425414364 15.26243093922652 21 23.41160220994475 27.002762430939224 29.005524861878452 20.58011049723757 22 19.751381215469614 26.243093922651934 35.290055248618785 18.715469613259668 23 22.23756906077348 27.071823204419886 30.939226519337016 19.751381215469614 24 22.997237569060776 29.21270718232044 28.45303867403315 19.337016574585636 25 24.585635359116022 30.31767955801105 23.756906077348066 21.33977900552486 26 25.20718232044199 29.41988950276243 24.79281767955801 20.58011049723757 27 19.55617198335645 28.224687933425795 26.629680998613036 25.589459084604716 28 19.488597097442987 28.127159640635796 28.47270214236351 23.911541119557704 29 26.403326403326403 28.8981288981289 23.90852390852391 20.79002079002079 30 24.654696132596683 24.378453038674035 32.527624309392266 18.439226519337016 31 26.519337016574585 27.831491712707184 27.071823204419886 18.577348066298345 32 25.12110726643599 29.134948096885815 26.574394463667822 19.16955017301038 33 29.903314917127073 25.483425414364643 25.828729281767952 18.784530386740332 34 21.201657458563535 26.381215469613263 33.70165745856354 18.715469613259668 35 22.375690607734807 28.03867403314917 31.353591160220994 18.23204419889503 36 19.75051975051975 30.97713097713098 29.72972972972973 19.542619542619544 37 22.513812154696133 31.560773480662984 27.900552486187845 18.024861878453038 38 20.64917127071823 32.251381215469614 28.52209944751381 18.577348066298345 39 25.483425414364643 30.593922651933703 24.102209944751383 19.82044198895028 40 20.718232044198896 29.21270718232044 29.35082872928177 20.718232044198896 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 2.0 12 4.0 13 4.0 14 5.0 15 6.0 16 7.5 17 9.0 18 9.0 19 10.0 20 11.0 21 9.0 22 7.0 23 7.0 24 11.5 25 16.0 26 19.5 27 23.0 28 23.0 29 25.0 30 27.0 31 34.0 32 41.0 33 41.0 34 49.0 35 57.0 36 67.0 37 77.0 38 77.0 39 95.5 40 114.0 41 119.5 42 125.0 43 125.0 44 123.0 45 121.0 46 112.0 47 103.0 48 103.0 49 109.5 50 116.0 51 128.5 52 141.0 53 141.0 54 131.0 55 121.0 56 132.5 57 144.0 58 144.0 59 120.0 60 96.0 61 70.0 62 44.0 63 44.0 64 33.5 65 23.0 66 16.0 67 9.0 68 9.0 69 8.5 70 8.0 71 5.0 72 2.0 73 2.0 74 1.5 75 1.0 76 1.5 77 2.0 78 2.0 79 1.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.06906077348066297 8 0.4143646408839779 9 0.20718232044198895 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.06906077348066297 18 0.0 19 0.06906077348066297 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.4143646408839779 28 0.06906077348066297 29 0.3453038674033149 30 0.0 31 0.0 32 0.20718232044198895 33 0.0 34 0.0 35 0.0 36 0.3453038674033149 37 0.0 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 1448.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.853591160221 #Duplication Level Percentage of deduplicated Percentage of total 1 96.32082413539366 90.40055248618785 2 2.501839587932303 4.696132596685083 3 0.44150110375275936 1.2430939226519337 4 0.515084621044886 1.9337016574585635 5 0.14716703458425312 0.6906077348066298 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.07358351729212656 1.0359116022099446 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 15 1.0359116022099446 No Hit TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG 5 0.3453038674033149 No Hit TCGTAAGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT 5 0.3453038674033149 No Hit TCGTAAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4 0.2762430939226519 No Hit TCGTAAGGGGGGGAGGTAGTGACGAAAAATAACAATACAG 4 0.2762430939226519 No Hit TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG 4 0.2762430939226519 No Hit TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA 4 0.2762430939226519 No Hit TCGTAAGGGGTGTTCTTTATTAATATCCTAACACTCCTCG 4 0.2762430939226519 No Hit TCGTAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 4 0.2762430939226519 No Hit TCGTAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 4 0.2762430939226519 No Hit TCGTAAGGGATAGCAGGATTTGTTCACTGATTCCCATTAT 3 0.20718232044198895 No Hit TCGTAAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACT 3 0.20718232044198895 No Hit TCGTAAGGGACTAATCCTAGCCCTAGCCCTACACAAATAT 3 0.20718232044198895 No Hit TCGTAAGGGCGGGGAGGTAGTGACGAAAAATAACAATACA 3 0.20718232044198895 No Hit TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGA 3 0.20718232044198895 No Hit TCGTAAGGGGTCTTTCCTGCCCTCTGTCGTCTGTTTTAAG 3 0.20718232044198895 No Hit TCGTAAGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGC 2 0.13812154696132595 No Hit TCGTAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG 2 0.13812154696132595 No Hit TCGTAAGGGGTTGCTTACATTTTACTGTACATTTTTCTGA 2 0.13812154696132595 No Hit TCGTAAGGGGATTCCGGAGAGGGAGCCTGAGAAACGGCTA 2 0.13812154696132595 No Hit TCGTAAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT 2 0.13812154696132595 No Hit TCGTAAGGGGGGAGACAACTACTGCCAAATGAATAAACTC 2 0.13812154696132595 No Hit TCGTAAGGGTCACAGACCCTCTTCTGAGATGAGTTTTTGT 2 0.13812154696132595 No Hit TCGTAAGGGCGTAGGAGTAAACATAACATTCTTCCCTCAA 2 0.13812154696132595 No Hit TCGTAAGGGGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA 2 0.13812154696132595 No Hit TCGTAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG 2 0.13812154696132595 No Hit TCGTAAGGGGATGTGCAGCTAGGCATTAAGGACCCCTCTG 2 0.13812154696132595 No Hit TCGTAAGGGGACCATGCTGTGTTAGGGGATGTGTTCCAGG 2 0.13812154696132595 No Hit TCGTAAGNGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 2 0.13812154696132595 No Hit TCGTAAAGGCAAACCTTTATTTATAAGTGTGATGCAGTTC 2 0.13812154696132595 No Hit TCGTAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.13812154696132595 No Hit TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC 2 0.13812154696132595 No Hit TCGTAAGGGGTAAATCATTAATCTTCTGACAAGAAGCTGA 2 0.13812154696132595 No Hit TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAAAG 2 0.13812154696132595 No Hit TCGTAAGGGATGCCACAACTAGATACATCAACATGATTTA 2 0.13812154696132595 No Hit TCGTAAGGGGGGGCTGGTGAGATGGCTCAGGTGGGTAAGA 2 0.13812154696132595 No Hit TCGTAAGGGGGCAGATCCTGGCACTCACTGACACCAGTCT 2 0.13812154696132595 No Hit TCGTAAGGGTGTTTGTTTTTCCTTAGAATGGATTTTGGTT 2 0.13812154696132595 No Hit TCGTAAGGGGTCTAAGTACGCACGGCCGGTACAGTGAAAC 2 0.13812154696132595 No Hit TCGTAAGGGTGTTCTTTATTAATATCCTAACACTCCTCGT 2 0.13812154696132595 No Hit TCGTAAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAG 2 0.13812154696132595 No Hit TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAAC 2 0.13812154696132595 No Hit TCGTAAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCAC 2 0.13812154696132595 No Hit TCGTAAGGGGGGGCTGGTGAGATGGCTTAGTGGGTAAGAG 2 0.13812154696132595 No Hit TCGTAAGGGATTCGTAGGAGTAAACATAACATTCTTCCCT 2 0.13812154696132595 No Hit TCGTAAGGGGGGTCAGGAGTTTCACCCATTGCTTCTTCAA 2 0.13812154696132595 No Hit TCGTAAGGGGGGTTTCTATCATCCTGTCTCTGTTTAAAAG 2 0.13812154696132595 No Hit TCGTAAGGGGGAGCTATTTTAATATGTTGTATAAAGCGAA 2 0.13812154696132595 No Hit TCGTAAGGGGTTAGGGGGCAGATAAAGTGGTCAGGCTGAG 2 0.13812154696132595 No Hit TCGTAAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA 2 0.13812154696132595 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGGGGG 25 1.1732582E-4 34.0 6 AAGGGGG 30 6.8233385E-6 34.0 5 CGTAAGG 125 0.0 34.0 2 TCGTAAG 130 0.0 34.0 1 TAAGGGG 65 0.0 34.0 4 TAAGGGA 25 1.1732582E-4 34.0 4 AAGGGGT 20 0.0019453749 33.999996 5 TAAGGGT 20 0.0019453749 33.999996 4 GTAAGGG 125 0.0 32.64 3 >>END_MODULE