FastQCFastQC Report
Fri 17 Jun 2016
SRR1525850_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525850_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65951
Sequences flagged as poor quality0
Sequence length56
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT12921.9590301890797714No Hit
AATAAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC9281.4071052751285045No Hit
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4420.6701945383693955No Hit
AATAAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC3580.5428272505344878No Hit
AATAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG3000.4548831708389562No Hit
AATAAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT2950.44730178465830694No Hit
AATAAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA2390.3623902594350351No Hit
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA2230.33812982365695743No Hit
AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1620.24563691225303633No Hit
AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1590.2410880805446468No Hit
AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1580.23957180330851693No Hit
AATAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1500.2274415854194781No Hit
AATAAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT1370.20772998134979No Hit
AATAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1250.18953465451623175No Hit
AATAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1160.17588815939106306No Hit
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT960.14556261466846598No Hit
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA940.14253006019620626No Hit
AATAAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT900.13646495125168687No Hit
AATAAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC890.13494867401555702No Hit
AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA860.13039984230716745No Hit
AATAAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC860.13039984230716745No Hit
AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.12433473336264803No Hit
AATAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG800.1213021788903883No Hit
AATAAAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT750.11372079270973905No Hit
AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA750.11372079270973905No Hit
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA740.11220451547360918No Hit
AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA690.10462312929295993No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA203.9100597E-450.61835540
CTACAAA150.00799728850.6183540
CATTGAA600.050.57935341
AATTATA354.7364665E-850.5793541
TTGTCTA150.008046200550.54041737
GGCGCAT650.050.54041737
AATAACG150.00809533550.46272336
ATTGCTT204.000267E-450.38526534
TAGAGCG251.9739698E-550.38526534
ATTATTA150.00831920950.1160347
AACAATT150.00834436550.07780543
GCCCAAG204.1231076E-450.07780548
CAACAAT150.00834436550.07780542
CCCACAA150.00834436550.07780514
ACATCCT150.00834436550.07780514
TAATCCG150.00834436550.07780542
CCCTCCT204.1231076E-450.07780514
AGATTAA650.050.07780549
TTGCCTA204.1231076E-450.07780542
ACAACAC150.00834436550.07780543
Produced by FastQC (version 0.11.5)