##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525850_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 65951 Sequences flagged as poor quality 0 Sequence length 56 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.38853087898591 39.0 37.0 39.0 34.0 40.0 2 36.682097314673015 39.0 37.0 39.0 32.0 40.0 3 36.367409137086625 39.0 36.0 39.0 31.0 40.0 4 35.55565495595214 39.0 35.0 39.0 25.0 40.0 5 35.52375248290397 39.0 35.0 39.0 27.0 40.0 6 31.142636199602734 36.0 27.0 39.0 12.0 40.0 7 32.47746053888493 36.0 31.0 39.0 19.0 40.0 8 33.84662855756547 38.0 33.0 39.0 22.0 40.0 9 33.786356537429306 38.0 33.0 39.0 21.0 40.0 10 32.968143015268915 38.0 32.0 39.0 17.0 40.0 11 32.09557095419326 37.0 30.0 39.0 15.0 40.0 12 31.17678276296038 36.0 27.0 39.0 11.0 40.0 13 30.283013146123636 35.0 25.0 39.0 10.0 40.0 14 31.312641203317614 36.0 28.0 39.0 12.0 40.0 15 29.888568785916817 35.0 25.0 38.0 8.0 40.0 16 30.982289881882004 36.0 27.0 39.0 10.0 40.0 17 29.365043744598264 35.0 23.0 38.0 10.0 40.0 18 28.834392200269896 34.0 20.0 38.0 10.0 40.0 19 30.189125259662475 35.0 25.0 38.0 11.0 40.0 20 31.053630725841913 35.0 27.0 38.0 13.0 40.0 21 31.724371124016315 37.0 29.0 39.0 12.0 40.0 22 30.843838607450987 36.0 27.0 39.0 10.0 40.0 23 31.78643235129111 37.0 29.0 39.0 11.0 40.0 24 30.979879001076558 36.0 27.0 39.0 10.0 40.0 25 31.64843596003093 37.0 29.0 39.0 10.0 40.0 26 31.93683189034283 38.0 30.0 39.0 7.0 40.0 27 32.25888917529681 38.0 31.0 39.0 4.0 40.0 28 32.18487968340131 38.0 31.0 39.0 4.0 40.0 29 31.748161513851194 37.0 30.0 39.0 4.0 40.0 30 31.50349501902928 37.0 29.0 39.0 4.0 40.0 31 31.980000303255448 38.0 31.0 39.0 4.0 40.0 32 31.87390638504344 38.0 31.0 39.0 4.0 40.0 33 31.63791299601219 37.0 31.0 39.0 4.0 40.0 34 31.402768722233173 37.0 30.0 39.0 4.0 40.0 35 31.38953162196176 37.0 31.0 39.0 4.0 40.0 36 30.210262164334125 36.0 28.0 39.0 4.0 40.0 37 30.03491986474807 36.0 28.0 39.0 4.0 40.0 38 29.4778244454216 35.0 27.0 38.0 4.0 40.0 39 28.444633136722718 35.0 23.0 38.0 4.0 40.0 40 26.231823626631893 33.0 11.0 38.0 4.0 39.0 41 24.289972858637473 30.0 8.0 37.0 4.0 39.0 42 25.10698852178132 30.0 10.0 37.0 4.0 39.0 43 25.29982866067232 31.0 8.0 37.0 4.0 39.0 44 24.559460811814834 30.0 5.0 37.0 4.0 39.0 45 25.319904171278676 31.0 4.0 37.0 4.0 39.0 46 23.754636017649467 29.0 4.0 36.0 4.0 39.0 47 22.912450152385862 28.0 4.0 35.0 4.0 39.0 48 23.347621719155132 28.0 4.0 36.0 4.0 39.0 49 22.550984822064866 27.0 4.0 35.0 4.0 39.0 50 23.2416945914391 28.0 4.0 36.0 4.0 39.0 51 23.54276659944504 30.0 4.0 36.0 4.0 39.0 52 23.415975496959863 30.0 4.0 36.0 4.0 39.0 53 23.249715698018225 30.0 4.0 36.0 4.0 39.0 54 22.975769889766646 29.0 4.0 36.0 4.0 39.0 55 22.82717472062592 29.0 4.0 36.0 4.0 39.0 56 21.866704068171824 27.0 4.0 36.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.303261178909061 1 2 2.249465714300541 1 3 2.675271229944663 1 4 3.6034982103879685 1 5 3.759799439680222 1 6 8.685473582602402 1 7 6.599540423796569 1 8 5.770518752583463 1 9 5.904902029078787 1 10 6.318952787779718 1 11 2.6413639272076708 1 12 -3.6285680704569074 1 13 -14.924262969241122 1 14 -3.7377430081753857 1 15 3.330988204894364 1 16 -1.5041744901731349 1 17 -4.213129698886878 1 18 -2.2952503887169584 1 19 1.1740064628510432 1 20 -0.49539232759187257 1 21 7.0673518694273305 1 22 8.157181012392368 1 23 6.997584873807959 1 24 7.681198875756451 1 25 6.463723962033317 1 26 6.972585450176322 1 27 5.772385024620185 1 28 6.078707159547022 1 29 6.908538260984301 1 30 6.476626025981549 1 31 5.756839671511919 1 32 5.360136948738081 1 33 4.82401335038767 1 34 5.482292902949265 1 35 6.050786427485928 1 36 4.094631666371178 1 37 4.440169318934057 1 38 4.759990963807308 1 39 4.915818435542796 1 40 -9.676530654640516 1 41 -1.3140129614459397 1 42 -2.65867984954372 1 43 -8.186200944673473 1 44 -14.224577787074887 1 45 -2.561471664736395 1 46 -6.685705891789443 1 47 -11.740435048303217 1 48 -6.387068562389459 1 49 -12.882825507034436 1 50 -4.562655824619057 1 51 -0.41351856814386423 1 52 -0.36162658949429627 1 53 -0.5216837381694717 1 54 -0.4586982975619698 1 55 -0.8340004289454654 1 56 2.7605447531986442 2 1 2.0305609410026335 2 2 2.7205204327779384 2 3 3.5118096914831227 2 4 4.58912469730312 2 5 4.5324996775866495 2 6 9.742174613530235 2 7 7.545813223162149 2 8 6.828310187952219 2 9 6.881012259055002 2 10 7.445737085955766 2 11 7.343980897865883 2 12 4.4519236028182725 2 13 5.354186673899246 2 14 7.722506793569259 2 15 8.678232455489134 2 16 6.294695454315363 2 17 8.883024147266969 2 18 6.935221459022934 2 19 7.488935090924002 2 20 6.53355295393073 2 21 7.362058451505053 2 22 8.15995658733289 2 23 7.169274009414622 2 24 8.267935592647802 2 25 7.067808022302945 2 26 6.620682198788533 2 27 6.5089036606233535 2 28 6.867961719420144 2 29 6.593312249882004 2 30 6.96879493954221 2 31 5.33624094034618 2 32 6.434978344455764 2 33 5.567669179095045 2 34 5.671428509610646 2 35 5.456615134861021 2 36 6.153810889210192 2 37 6.269867970797659 2 38 6.432671376178444 2 39 7.210822400649853 2 40 6.500114864788507 2 41 9.270344691210681 2 42 8.073080657989983 2 43 7.34670944390702 2 44 -2.3424405943706823 2 45 6.825225401084381 2 46 5.595816709320786 2 47 1.5382128501900425 2 48 5.177364427301264 2 49 -1.0518381953770195 2 50 4.8784623355712675 2 51 8.297127744306572 2 52 7.500189429538217 2 53 8.14455337523259 2 54 8.486682352715587 2 55 7.7942707843772965 2 56 9.108185514499198 3 1 2.2802862157279122 3 2 3.1051358173933252 3 3 3.7705093251827577 3 4 4.2566155397939625 3 5 4.716107736194708 3 6 9.861222232577855 3 7 7.582260109609038 3 8 6.038658173300206 3 9 6.864254017296755 3 10 7.154986170204854 3 11 7.27072082460581 3 12 8.04349869439336 3 13 9.031750776463344 3 14 8.262158808221272 3 15 5.070998023254699 3 16 8.04094087556079 3 17 9.882108396351217 3 18 10.014617063418534 3 19 8.662469889458798 3 20 7.867985188362965 3 21 8.226802041248643 3 22 8.92222764960395 3 23 8.234109174249788 3 24 8.741287240999455 3 25 8.114877619372546 3 26 7.410975239081576 3 27 6.743519045238742 3 28 6.880965382423803 3 29 7.339466096035849 3 30 6.436468932216204 3 31 5.593658522763761 3 32 5.062542447019865 3 33 5.748804710230576 3 34 5.5970695352516735 3 35 5.46119388943978 3 36 7.688151548550856 3 37 7.4623588132885 3 38 8.077909471416536 3 39 8.279686869514322 3 40 10.490774205447845 3 41 11.910729306595297 3 42 10.60366673857606 3 43 10.512277209474782 3 44 10.462412885482799 3 45 10.23136093221991 3 46 11.973106086610162 3 47 11.542333729310918 3 48 11.351173951110788 3 49 12.148985980447154 3 50 11.114909222018156 3 51 10.706559978738806 3 52 9.937735217084004 3 53 10.081458137137354 3 54 10.710766601799836 3 55 10.671743311849823 3 56 10.087397968711652 4 1 1.671714787156482 4 2 2.815135817393326 4 3 3.2652712299446662 4 4 4.0356631588415794 4 5 4.341345831432804 4 6 9.322174613530233 4 7 6.632736300085227 4 8 5.864848649490682 4 9 6.451396874439613 4 10 6.676890932109611 4 11 7.601673205558193 4 12 8.087308218202889 4 13 8.788417443130012 4 14 8.0959683320308 4 15 8.61861707087375 4 16 8.190464685084596 4 17 10.29687030111312 4 18 11.011759920561396 4 19 9.440088937077846 4 20 8.408937569315341 4 21 7.920135374581971 4 22 8.528418125794428 4 23 6.856966317106931 4 24 8.559858669570886 4 25 7.470115714610639 4 26 6.141451429557765 4 27 5.933519045238739 4 28 6.133346334804756 4 29 6.8594660960358524 4 30 6.844564170311447 4 31 5.955087094192336 4 32 5.769209113686529 4 33 5.696899948325811 4 34 5.343736201918336 4 35 5.8773843656302525 4 36 6.129580119979423 4 37 6.391406432336115 4 38 7.112671376178444 4 39 7.581591631419087 4 40 3.7293456340192783 4 41 11.910729306595297 4 42 11.044619119528441 4 43 10.3913248285224 4 44 11.1371747902447 4 45 9.859456170315152 4 46 12.391201324705403 4 47 12.720905157882349 4 48 12.147364427301262 4 49 12.944700266161437 4 50 12.745385412494343 4 51 11.74751235969119 4 52 12.012497121845911 4 53 11.87955337523259 4 54 11.925528506561736 4 55 11.828886168992678 4 56 12.025493206806892 5 1 2.2031710978360906 5 2 3.2313494096263327 5 3 3.6170188027601995 5 4 4.714498110297889 5 5 4.8598895207531925 5 6 9.727611506734117 5 7 7.654289698143479 5 8 6.468246707743106 5 9 7.082561922983306 5 10 7.033395786478547 5 11 7.942935341480521 5 12 8.227502392960165 5 13 8.906184433421277 5 14 8.405385807758954 5 15 9.69531609999996 5 16 7.834930704502074 5 17 10.388812048685939 5 18 10.951371571046828 5 19 8.710088937077849 5 20 8.037287083878454 5 21 8.014504306620811 5 22 8.382884145211907 5 23 7.5400731132234355 5 24 8.377625659862147 5 25 7.650892413639763 5 26 6.368441720819899 5 27 5.929926812229034 5 28 5.747909441600875 5 29 6.424902989239733 5 30 6.977476791670664 5 31 5.49130068642534 5 32 5.533092608832163 5 33 5.5868999483258115 5 34 5.999658532015427 5 35 5.96651057922249 5 36 6.910065556872631 5 37 7.073639442044854 5 38 8.056457783945437 5 39 8.829941145982197 5 40 10.06303495440763 5 41 12.501020568731217 5 42 11.580735624382811 5 43 11.168218032405896 5 44 11.914747605778679 5 45 10.916058112062725 5 46 11.793725596550065 5 47 12.6019731190474 5 48 11.60212170885466 5 49 12.597807062277948 5 50 12.200531043562307 5 51 10.753531777166916 5 52 11.005603917962418 5 53 10.956446579116083 5 54 10.869897438600574 5 55 10.608497819478117 5 56 10.265299032049608 6 1 2.110300645742342 6 2 2.9233176355751453 6 3 3.442947997621431 6 4 4.271622754801179 6 5 4.720436740523709 6 6 9.615911987267612 6 7 7.473746401095333 6 8 6.015858750500783 6 9 6.62644737949012 6 10 6.512850528069212 6 11 7.328440882325864 6 12 8.132964783859453 6 13 9.720639665352234 6 14 7.820311766374232 6 15 9.847910000166674 6 16 7.872686907306818 6 17 9.837375351618174 6 18 10.837416486217961 6 19 8.992917219906133 6 20 8.325705246083018 6 21 7.90761012205672 6 22 8.58225650963281 6 23 7.4315117716523815 6 24 8.141575841288052 6 25 7.626277330772258 6 26 6.7125625406688805 6 27 6.2546301563498545 6 28 6.102033203491629 6 29 6.121284277854031 6 30 6.4280995238468 6 31 5.7186224477276895 6 32 5.692845477322898 6 33 5.859627221053085 6 34 6.1869685251506645 6 35 6.63435406259995 6 36 7.209075069474373 6 37 7.137972088901773 6 38 7.041257234764306 6 39 7.997147186974644 6 40 3.0445981592718034 6 41 10.9147697106357 6 42 9.542194877104201 6 43 9.852536949734528 6 44 9.75303337610329 6 45 9.035112735971719 6 46 11.170797284301361 6 47 12.19312738010457 6 48 10.98291998285682 6 49 12.418538649999821 6 50 11.61447632158525 6 51 11.004684076862905 6 52 10.725325404674194 6 53 9.650866506545722 6 54 11.076134567167802 6 55 10.716764956871469 6 56 10.066907348221033 7 1 1.8001018839306724 7 2 2.589006785135261 7 3 2.944088434245735 7 4 4.267061008303948 7 5 3.929625401325275 7 6 9.059378914605507 7 7 6.448005117289533 7 8 5.936354025834767 7 9 6.089138809923487 7 10 6.626568351464453 7 11 6.307587184052814 7 12 7.244727573041597 7 13 7.939277658183773 7 14 6.547258654611447 7 15 8.800337500981275 7 16 7.102077588310404 7 17 8.840633741973338 7 18 9.217996479701178 7 19 7.430519044604729 7 20 6.960765526304591 7 21 6.246156879958317 7 22 7.359708448375077 7 23 6.267288897752088 7 24 7.1956651211837865 7 25 6.139147972675158 7 26 6.031558956439486 7 27 5.509110443088204 7 28 5.943023754159597 7 29 6.2190359885089705 7 30 5.805746966010371 7 31 4.461861287740717 7 32 5.361467178202659 7 33 4.447115002089252 7 34 4.831478137402211 7 35 5.518029526920575 7 36 6.829472593097705 7 37 6.621191378572679 7 38 7.161165999834356 7 39 7.968473351849191 7 40 6.884937031868741 7 41 10.435460489390994 7 42 10.333006216302635 7 43 9.774335581210579 7 44 10.468250059061909 7 45 9.286660471390423 7 46 11.049911002124755 7 47 11.562303007344717 7 48 11.040267653107712 7 49 11.830399190892624 7 50 10.673127347978216 7 51 10.330523112379364 7 52 10.141421853028707 7 53 10.218478106415386 7 54 10.44563603344346 7 55 9.606305523831388 7 56 9.699686755193987 8 1 1.7623530850288205 8 2 2.243433689733749 8 3 2.6646329320723225 8 4 3.6969397545862606 8 5 3.811345831432803 8 6 8.827280996508957 8 7 5.713161832000125 8 8 5.297827372894936 8 9 5.95118410848217 8 10 6.023273910833016 8 11 7.16231150343053 8 12 7.439648643734802 8 13 8.38139616653427 8 14 7.332776842669098 8 15 7.705851113426942 8 16 7.097273195722895 8 17 8.850700088347164 8 18 9.282398218433734 8 19 7.880301703035293 8 20 6.51063969697492 8 21 7.266518353305376 8 22 8.006928764092304 8 23 6.828030146894164 8 24 7.129433137655987 8 25 6.148413586951062 8 26 6.505706748706704 8 27 5.941391385664275 8 28 5.786537824166459 8 29 5.833083117312448 8 30 6.404351404353999 8 31 5.035299860149777 8 32 4.541975071133336 8 33 5.3528573951343255 8 34 5.619055350854513 8 35 5.341639684779192 8 36 6.238303524234745 8 37 6.470768134463778 8 38 7.140118184689083 8 39 6.430953333546746 8 40 3.2321115914660865 8 41 10.234133561914444 8 42 9.901640396124186 8 43 9.752601424267084 8 44 9.794834364712784 8 45 9.246051914996006 8 46 12.051626856620299 8 47 12.49218175362703 8 48 11.586938895386368 8 49 11.843210904459315 8 50 11.758576901856046 8 51 10.532193210755015 8 52 10.39994393035655 8 53 10.51955337523259 8 54 10.707017868263868 8 55 11.205481913673534 8 56 10.829748525955829 9 1 1.422169332611027 9 2 2.5142267264842317 9 3 3.050271229944663 9 4 4.164299522477947 9 5 4.29998219506917 9 6 8.889901886257508 9 7 7.281827209176136 9 8 6.210303194945226 9 9 6.757760510803248 9 10 6.65552729574598 9 11 7.45849138737637 9 12 7.862762763657432 9 13 8.941599261311833 9 14 8.19278651384898 9 15 9.50952616178284 9 16 8.275464685084597 9 17 9.980052119294943 9 18 10.12403264783412 9 19 8.891907118896029 9 20 8.535301205678977 9 21 7.160135374581973 9 22 8.64159994397625 9 23 6.840602680743295 9 24 7.560767760479976 9 25 6.885115714610638 9 26 6.31736052046686 9 27 5.904882681602373 9 28 6.381073607532031 9 29 6.621284277854031 9 30 6.279109624856897 9 31 5.3537234578287 9 32 4.942845477322898 9 33 5.439172675598538 9 34 4.966008929191066 9 35 5.016929820175708 9 36 5.930034665433972 9 37 6.231406432336119 9 38 6.875853194360264 9 39 6.668864358691813 9 40 3.2238910885647307 9 41 8.090274761140751 9 42 8.182346392255717 9 43 8.799506646704224 9 44 9.360356608426521 9 45 8.367183443042425 9 46 10.779383142887223 9 47 10.925450612427802 9 48 10.54100079093763 9 49 10.69379117525235 9 50 10.159930867039797 9 51 10.082966905145735 9 52 9.783406212755 9 53 10.099098829778043 9 54 9.98143759747083 9 55 9.815249805356316 9 56 9.811856843170528 10 1 2.0406036760453716 10 2 3.0829135951710995 10 3 3.5086045632779985 10 4 4.531218714397134 10 5 4.544679164766137 10 6 9.59773016908579 10 7 7.050514077863006 10 8 5.814848649490678 10 9 6.1991746522173905 10 10 7.050224265442946 10 11 7.251673205558191 10 12 8.345085995980664 10 13 9.309528554241123 10 14 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5.504341428191573 114 25 4.406667438748567 114 26 4.342830739902592 114 27 4.384898355583566 114 28 3.8195532313564797 114 29 4.473948854656541 114 30 4.040081411690753 114 31 4.106466404537159 114 32 3.9498987688589438 114 33 4.431727534532708 114 34 4.674081029504546 114 35 3.868418848388874 114 36 5.897511154462187 114 37 5.740027121991293 114 38 5.941292065833618 114 39 6.493315769350119 114 40 8.484862875398587 114 41 7.114177582457366 114 42 6.888067395390511 114 43 6.886497242315507 114 44 -2.5224803821690926 114 45 5.866007894453084 114 46 -0.3577641925359778 114 47 -2.891163807634893 114 48 0.32391615143919594 114 49 -7.86185145797649 114 50 0.7915923090460666 114 51 2.856477876932569 114 52 2.356290225294188 114 53 3.3988637200601772 114 54 3.8603560927686367 114 55 3.0668172034754377 114 56 5.546182861979304 115 1 1.2178164820717328 115 2 2.1559832750204464 115 3 2.5905254672328013 115 4 3.6417648537568326 115 5 3.7774475263480554 115 6 7.581157664377695 115 7 4.660871893305568 115 8 4.202984242711018 115 9 4.341735857490459 115 10 4.7135011016011354 115 11 1.0584528665751378 115 12 -0.8055731377293185 115 13 -2.2134469636496483 115 14 -3.6987774306810657 115 15 -4.674603268109305 115 16 1.2606341766100186 115 17 2.3800906400961743 115 18 3.4920989036122414 115 19 2.1338177506371707 115 20 1.7336833320272085 115 21 4.719457408480277 115 22 5.045875752913073 115 23 4.57018665608998 115 24 4.596977313638682 115 25 4.230454697661482 115 26 3.9912819380323405 115 27 3.516400401170941 115 28 3.148600572092896 115 29 3.447940672307034 115 30 4.633886204209748 115 31 3.3481379416499593 115 32 3.2444633509746694 115 33 3.0106287618851333 115 34 2.643397218867495 115 35 3.03907928088449 115 36 4.056190289470948 115 37 3.3627623645395097 115 38 4.725044257534375 115 39 4.628032309385187 115 40 7.960023600120973 115 41 7.629373374391907 115 42 7.021907255121665 115 43 5.155053642081725 115 44 -1.510791311450216 115 45 4.6389476957388815 115 46 0.14340471453591164 115 47 -7.791806706524431 115 48 0.5121101900131286 115 49 -2.6941132931605942 115 50 1.807080327748583 115 51 3.101071681725088 115 52 2.8662259354052324 115 53 1.7737906633681817 115 54 2.693494608256657 115 55 2.0644793893316624 115 56 4.8746457491797734 116 1 1.99838145382315 116 2 2.825135817393324 116 3 3.301937896611328 116 4 4.2089964921749115 116 5 4.5580124980994725 116 6 9.1888412801969 116 7 6.959402966751895 116 8 5.701515316157348 116 9 6.13473020777295 116 10 6.676890932109611 116 11 5.838339872224857 116 12 5.713974884869554 116 13 7.151750776463349 116 14 6.479301665364133 116 15 -3.3880495957929213 116 16 4.4904646850846 116 17 6.920203634446455 116 18 6.938426587228058 116 19 6.750088937077848 116 20 5.3422709026486785 116 21 6.9734687079153055 116 22 7.811751459127766 116 23 6.723632983773598 116 24 7.8631920029042135 116 25 7.1967823812773055 116 26 6.361451429557764 116 27 6.183519045238739 116 28 5.36667966813809 116 29 6.899466096035852 116 30 6.314564170311446 116 31 5.125087094192331 116 32 5.36920911368653 116 33 5.560233281659148 116 34 5.4237362019183415 116 35 5.897384365630256 116 36 7.832913453312756 116 37 7.708073099002785 116 38 8.636004709511777 116 39 9.414924964752423 116 40 10.826012300685942 116 41 9.657395973261966 116 42 7.517952452861778 116 43 2.837991495189069 116 44 4.6871747902447005 116 45 7.682789503648486 116 46 2.837867991372068 116 47 -2.4024281754509857 116 48 0.5473644273012646 116 49 2.141366932828106 116 50 3.1453854124943454 116 51 6.6208456930245205 116 52 6.882497121845912 116 53 6.4662200418992555 116 54 6.682195173228408 116 55 6.92888616899268 116 56 7.858826540140225 117 1 0.42818537539177726 117 2 1.3404299350403832 117 3 1.3615457397485855 117 4 2.5384082568807926 117 5 2.713306615746525 117 6 6.595115790000822 117 7 4.392344143222488 117 8 3.1893584534122468 117 9 3.6900243254200085 117 10 3.7600281870115744 117 11 1.1810849702640738 117 12 -4.824848644542211 117 13 -7.462366870595478 117 14 -2.3983453934593975 117 15 -4.297069203636056 117 16 -8.329927471778149 117 17 0.00255657562292555 117 18 -1.2184361578699772 117 19 1.063030113548436 117 20 -0.5079251757826952 117 21 3.3169981196800116 117 22 4.512143615990507 117 23 3.016182003381438 117 24 3.577701806825786 117 25 3.421880420492993 117 26 3.758314174655805 117 27 3.6729308099446243 117 28 3.2921698642165254 117 29 3.9198582528985924 117 30 3.5843680918800693 117 31 2.4631263098786107 117 32 2.7017581332943763 117 33 2.0574881836199275 117 34 2.8833440450555976 117 35 3.5060118166106484 117 36 4.534089923900993 117 37 4.932386824492983 117 38 5.712083140884328 117 39 4.716101435340654 117 40 7.735031908529084 117 41 6.157788130124711 117 42 3.804226962665698 117 43 -3.2882830146148514 117 44 -1.832825209755299 117 45 3.9988679350210354 117 46 -1.3966418125495004 117 47 -1.891839940156867 117 48 -1.2393022393654043 117 49 -0.3182409103091466 117 50 0.7053854124943442 117 51 4.05221824204413 117 52 4.011124572826304 117 53 3.5979847477816094 117 54 3.3880775261695817 117 55 3.262219502326012 117 56 5.650983402885323 118 1 -0.4089994985578045 118 2 0.7122786745361793 118 3 0.9252712299446628 118 4 1.80066315884158 118 5 2.168488688575657 118 6 6.188603184958804 118 7 4.104879157228083 118 8 3.932705792347825 118 9 4.1571111601538995 118 10 4.452605217823898 118 11 1.294530348415332 118 12 -4.64697749608283 118 13 -5.126582556869987 118 14 -4.953317382254916 118 15 -9.367097214840538 118 16 -7.438821029201119 118 17 0.0011560153988376953 118 18 -5.299668650867179 118 19 -0.5220539200650087 118 20 -0.8867767163989413 118 21 3.767278231724827 118 22 5.484132411508714 118 23 4.155537745678359 118 24 4.7377158124280285 118 25 3.902972857467784 118 26 4.387165715272051 118 27 3.8756619023815944 118 28 3.5547749062333267 118 29 3.8323232388929966 118 30 4.735278456025728 118 31 3.615087094192333 118 32 3.8649233994008156 118 33 3.747614234040096 118 34 2.863736201918339 118 35 1.7409557942016818 118 36 3.527437262836571 118 37 3.2849778609075457 118 38 4.343385661892729 118 39 5.120163059990514 118 40 6.21577420544785 118 41 5.546443592309583 118 42 4.981047690957016 118 43 1.1420391142366881 118 44 -0.33282520975529906 118 45 4.165884741743724 118 46 -1.467370103866024 118 47 -1.3505234135462238 118 48 -0.8047784298415941 118 49 -0.9295854481242749 118 50 0.8839568410657712 118 51 4.662512359691188 118 52 4.835354264703053 118 53 3.215981946661163 118 54 5.319099935133167 118 55 4.732457597564107 118 56 7.300493206806891 119 1 0.4079647871564802 119 2 1.2613858173933252 119 3 1.5502712299446628 119 4 2.69128815884158 119 5 2.686345831432803 119 6 7.054674613530235 119 7 5.247736300085229 119 8 4.852348649490679 119 9 4.762021874439615 119 10 5.182515932109613 119 11 1.5891732055581897 119 12 -3.9326917817971143 119 13 -0.40783255686998743 119 14 -3.8171566679692006 119 15 -6.518882929126253 119 16 -6.0526603149154035 119 17 0.5681203011131224 119 18 -2.6188650794386064 119 19 -0.5711610629221511 119 20 -0.3979374306846566 119 21 3.628885374581973 119 22 4.901543125794429 119 23 3.8207163171069283 119 24 3.4229836695708826 119 25 3.619490714610638 119 26 4.070201429557766 119 27 4.01851904523874 119 28 3.4520963348047573 119 29 3.9394660960358507 119 30 4.465189170311444 119 31 3.661962094192333 119 32 3.172959113686531 119 33 3.4931499483258115 119 34 3.441861201918339 119 35 3.6717593656302547 119 36 4.681455119979425 119 37 5.043906432336119 119 38 5.432671376178444 119 39 6.425966631419087 119 40 7.5974706340192775 119 41 6.291979306595298 119 42 4.097119119528443 119 43 0.7469498285224034 119 44 2.698424790244701 119 45 5.031956170315151 119 46 0.742451324705403 119 47 0.2990301578823491 119 48 -5.088260572698736 119 49 -0.8871747338385596 119 50 1.6116354124943442 119 51 5.771887359691188 119 52 5.739372121845911 119 53 5.53517837523259 119 54 5.81240350656174 119 55 5.89763616899268 119 56 8.097368206806891 120 1 1.4870089048035382 120 2 2.3551358173933252 120 3 2.469388877003489 120 4 3.837427864723935 120 5 3.973110537315158 120 6 8.15393931941259 120 7 5.902148064791113 120 8 5.091319237725976 120 9 5.665514521498441 120 10 6.240420343874316 120 11 3.0487320290876028 120 12 -0.2415153112088788 120 13 -1.2221707921641034 120 14 0.1653800967366834 120 15 0.6244994238149246 120 16 -2.937770609033052 120 17 2.7574585364072384 120 18 0.6835246264437451 120 19 2.0336183488425554 120 20 1.9283493340212274 120 21 6.204253021640799 120 22 6.904300478735603 120 23 6.388731022989283 120 24 6.837505728394412 120 25 6.098351008728283 120 26 6.366157311910708 120 27 6.099401398179914 120 28 6.027463981863583 120 29 6.762995507800554 120 30 7.177505346782031 120 31 5.644498858898217 120 32 6.020385584274763 120 33 5.8221940659728695 120 34 5.951971496035984 120 35 6.2756196597479 120 36 6.494874237626483 120 37 6.981406432336119 120 38 7.579730199707857 120 39 8.8386504549485 120 40 9.46052210460751 120 41 -5.1510353992870535 120 42 -4.156557351059792 120 43 -7.180439877359948 120 44 -14.068119327402357 120 45 -2.3099555943907326 120 46 -6.435857498824008 120 47 -5.367330136235298 120 48 -4.425576749169323 120 49 -13.980005616191502 120 50 -2.265202822799772 120 51 2.243394712632366 120 52 2.236614768904733 120 53 1.8430827869972966 120 54 1.9714108595029174 120 55 1.8700626395809152 120 56 5.351963795042185 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1308.0 5 868.0 6 2027.0 7 382.0 8 423.0 9 347.0 10 215.0 11 256.0 12 216.0 13 204.0 14 302.0 15 361.0 16 442.0 17 568.0 18 672.0 19 820.0 20 933.0 21 1147.0 22 1413.0 23 1659.0 24 1764.0 25 1995.0 26 2109.0 27 2048.0 28 2237.0 29 2319.0 30 2461.0 31 2663.0 32 3069.0 33 3466.0 34 3651.0 35 4029.0 36 4453.0 37 4847.0 38 5252.0 39 5019.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 96.39277645524707 2.921866234022229 0.07126503009810313 0.6140922806325909 8 96.34880441539931 3.2933541568740425 0.13494867401555702 0.22289275371108852 9 95.78923426838514 3.169067475360121 0.5534495830174375 0.488248673237301 10 48.12663947476157 23.882882746281332 20.510682173128536 7.47979560582857 11 33.12459250049279 27.373352943852254 13.005109854285756 26.496944701369195 12 28.201240314779152 37.200345711209835 14.318205940774211 20.280208033236796 13 20.189231399069005 26.58488878106473 16.601719458385773 36.6241603614805 14 17.796545920456097 46.019014116541065 18.28781974496217 17.896620218040667 15 33.55422512682236 29.378608534040186 18.032387307100528 19.034779032036926 16 21.499681306340488 45.072388988375266 17.86202082131909 15.565908883965157 17 21.37776227382343 31.421291310876192 32.702895363475044 14.498051051825339 18 19.29311155251626 30.137526345316978 18.915558520719927 31.653803581446834 19 18.45166770313519 33.65590256184684 17.30801316567823 30.584416569339744 20 18.078890254822504 34.81916556633126 30.985445218473494 16.11649896037275 21 17.199132689420935 32.19814710921745 20.363603281223938 30.239116920137675 22 15.78141347363952 32.45743051659565 33.787205652681536 17.97395035708329 23 16.766993677123924 34.12988430804688 31.74326393837849 17.3598580764507 24 16.283795053753657 34.36746576900332 19.66822847958271 29.68051069766031 25 17.94801807539502 35.72468383222637 29.44378734115792 16.883510751220694 26 33.05115601674859 34.33309060015778 16.294981491595365 16.32077189149827 27 16.86995570119546 47.23587596334729 16.284361915164755 19.609806420292493 28 24.66337114089889 42.4410141323467 16.08691696488142 16.80869776187299 29 28.004068681777465 42.25204572712505 14.940260213453977 14.803625377643503 30 25.35909842406225 34.31418647332737 25.650320799648107 14.676394302962278 31 27.796312022321974 40.30995996603178 18.24123498726192 13.652493024384327 32 28.171256976462022 40.284518320795925 17.21517835476826 14.329046347973792 33 29.098111832918462 39.31910630805658 16.74609920466274 14.836682654362212 34 19.63309439922245 32.916717288300326 33.0047989308711 14.445389381606121 35 18.056356845717062 34.8267695017156 33.94771202137674 13.1691616311906 36 23.875910194174757 34.02002427184466 27.38622572815534 14.717839805825243 37 24.36691564948078 32.99022286998239 27.79194753142649 14.850913949110343 38 25.808166737558487 31.925624354378073 27.535395272528408 14.73081363553503 39 25.59251780210437 32.59645020724838 20.27390188725081 21.537130103396443 40 20.901570408428523 25.15100081043473 25.958377295594598 27.98905148554215 41 17.218654692025932 22.56739566889914 23.785805146439028 36.4281444926359 42 17.345512021641994 36.789872032584576 22.62530776011429 23.239308185659137 43 14.812618776130748 32.95933105283162 27.872291904218926 24.3557582668187 44 16.395189526564167 35.1575582280408 27.439488506622013 21.007763738773026 45 22.46977704878197 30.29129457505619 29.644310795212924 17.59461758094891 46 16.15788272872462 36.285649098790785 23.647425659745988 23.909042512738612 47 14.316180726929288 32.27614782317277 22.15519605154922 31.252475398348718 48 22.826928037142117 31.818112852569453 23.565825608035322 21.789133502253105 49 16.675026981014486 33.50053962028972 22.96958365634548 26.85484974235031 50 16.75045946808026 36.28658656986193 23.576408402569985 23.386545559487825 51 15.47126786257221 36.62663423532989 25.80723624201885 22.09486166007905 52 16.031890660592254 42.42369020501139 25.65831435079727 15.88610478359909 53 15.717650634450269 40.9892865283793 25.747283641060708 17.545779196109716 54 15.844578166788231 31.654206175540967 31.12235594456601 21.378859713104788 55 15.701085139365938 30.896075868567436 30.57083801939269 22.832000972673942 56 16.08084492059874 30.825925081680722 31.638933211762023 21.454296785958512 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 384.0 1 445.5 2 507.0 3 459.0 4 470.5 5 530.0 6 648.0 7 766.0 8 1051.0 9 1336.0 10 1348.5 11 1361.0 12 1245.5 13 987.0 14 844.0 15 786.0 16 728.0 17 755.5 18 783.0 19 780.0 20 777.0 21 780.5 22 810.0 23 836.0 24 862.0 25 888.0 26 944.5 27 1001.0 28 1046.0 29 1234.5 30 1378.0 31 1367.5 32 1357.0 33 1335.0 34 1313.0 35 1444.0 36 1575.0 37 1678.0 38 2316.5 39 2852.0 40 4230.5 41 5609.0 42 5779.0 43 5949.0 44 4922.0 45 3895.0 46 3619.5 47 3229.0 48 3114.0 49 2984.5 50 2855.0 51 2756.0 52 2657.0 53 2655.0 54 2686.5 55 2720.0 56 2575.0 57 2430.0 58 2080.0 59 1730.0 60 1568.0 61 1406.0 62 1196.0 63 858.5 64 731.0 65 641.5 66 552.0 67 483.5 68 415.0 69 304.5 70 194.0 71 147.5 72 81.0 73 61.0 74 80.0 75 99.0 76 65.5 77 32.0 78 17.5 79 2.5 80 2.0 81 1.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0015162772361298539 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.46701338872799497 16 0.08642780245940167 17 0.027292990250337372 18 0.0 19 0.033358099194856786 20 0.09400918864005095 21 0.0 22 0.0 23 0.0 24 0.0030325544722597077 25 0.007581386180649269 26 0.05306970326454488 27 0.05306970326454488 28 0.004548831708389562 29 0.12433473336264803 30 0.033358099194856786 31 0.010613940652908977 32 0.02274415854194781 33 0.1015905748207002 34 0.15617655532137498 35 0.1288835650710376 36 0.047004594320025475 37 0.1258510105987779 38 0.18650210004397205 39 0.13343239677942714 40 0.8400175888159391 41 1.0644266197631576 42 0.2319904171278677 43 0.2668647935588543 44 0.3957483586298919 45 0.1622416642658944 46 0.31235311064274995 47 0.4624645570196055 48 0.06368364391745386 49 0.24866946672529605 50 0.17285560491880334 51 0.25928340737820504 52 0.15314400084911525 53 0.2213764764749587 54 0.21682764476656913 55 0.2319904171278677 56 0.2213764764749587 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 65951.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.19450804385073 #Duplication Level Percentage of deduplicated Percentage of total 1 90.36607649171445 65.2393443617231 2 5.624514313317791 8.121180876711499 3 1.6382080524226577 3.5480887325438584 4 0.7560960241950728 2.18343922002699 5 0.34864427782328355 1.2585101059877788 6 0.2814357423392771 1.2190868978484026 7 0.15962027177451535 0.8066594896210822 8 0.14491840463738895 0.8369850343436794 9 0.11341440362926092 0.736910736759109 >10 0.49986348266229813 6.388075995815075 >50 0.03570453447587844 1.9529650801352518 >100 0.027303467540377626 4.342618004275902 >500 0.002100266733875202 1.4071052751285045 >1k 0.002100266733875202 1.9590301890797714 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AATAAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 1292 1.9590301890797714 No Hit AATAAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 928 1.4071052751285045 No Hit AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 442 0.6701945383693955 No Hit AATAAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 358 0.5428272505344878 No Hit AATAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 300 0.4548831708389562 No Hit AATAAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 295 0.44730178465830694 No Hit AATAAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA 239 0.3623902594350351 No Hit AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 223 0.33812982365695743 No Hit AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 162 0.24563691225303633 No Hit AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 159 0.2410880805446468 No Hit AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 158 0.23957180330851693 No Hit AATAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 150 0.2274415854194781 No Hit AATAAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT 137 0.20772998134979 No Hit AATAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 125 0.18953465451623175 No Hit AATAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 116 0.17588815939106306 No Hit AATAAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 96 0.14556261466846598 No Hit AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA 94 0.14253006019620626 No Hit AATAAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT 90 0.13646495125168687 No Hit AATAAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC 89 0.13494867401555702 No Hit AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 86 0.13039984230716745 No Hit AATAAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC 86 0.13039984230716745 No Hit AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 82 0.12433473336264803 No Hit AATAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 80 0.1213021788903883 No Hit AATAAAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 75 0.11372079270973905 No Hit AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 75 0.11372079270973905 No Hit AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 74 0.11220451547360918 No Hit AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 69 0.10462312929295993 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.021227881305817955 0.0 0.0 0.0 0.0 7 0.021227881305817955 0.0 0.0 0.0 0.0 8 0.021227881305817955 0.0 0.0 0.0 0.0 9 0.021227881305817955 0.0 0.0 0.0 0.0 10 0.028809267486467227 0.0 0.0 0.0 0.0 11 0.03790693090324635 0.0 0.0 0.0 0.0 12 0.043972039847765765 0.0 0.0 0.0 0.0 13 0.047004594320025475 0.0 0.0 0.0 0.0 14 0.06974875286197328 0.0 0.0 0.0 0.0 15 0.07126503009810314 0.0 0.0 0.0 0.0 16 0.07733013904262255 0.0 0.0 0.0 0.0 17 0.08187897075101211 0.0 0.0 0.0 0.0 18 0.08491152522327182 0.0 0.0 0.0 0.0 19 0.08642780245940168 0.0 0.0 0.0 0.0 20 0.09097663416779124 0.0 0.0 0.0 0.0 21 0.0955254658761808 0.0 0.0 0.0 0.0 22 0.10310685205683007 0.0 0.0 0.0 0.0 23 0.1122045154736092 0.0 0.0 0.0 0.0 24 0.11978590165425847 0.0 0.0 0.0 0.0 25 0.12736728783490772 0.0 0.0 0.0 0.0 26 0.14859516914072568 0.0 0.0 0.0 0.0 27 0.16982305044654364 0.0 0.0 0.0 0.0 28 0.19559976346075117 0.0 0.0 0.0 0.003032554472259708 29 0.20772998134978998 0.0 0.0 0.0 0.003032554472259708 30 0.21227881305817956 0.0 0.0 0.0 0.003032554472259708 31 0.22895786265560794 0.0 0.0 0.0 0.003032554472259708 32 0.2365392488362572 0.0 0.0 0.0 0.003032554472259708 33 0.24260435778077663 0.0 0.0 0.0 0.003032554472259708 34 0.2501857439614259 0.0 0.0 0.0 0.003032554472259708 35 0.25625085290594535 0.0 0.0 0.0 0.003032554472259708 36 0.26231596185046474 0.0 0.0 0.0 0.003032554472259708 37 0.26231596185046474 0.0 0.0 0.0 0.003032554472259708 38 0.26231596185046474 0.0 0.0 0.0 0.003032554472259708 39 0.2668647935588543 0.0 0.0 0.0 0.003032554472259708 40 0.27292990250337373 0.0 0.0 0.0 0.003032554472259708 41 0.2820275659201528 0.0 0.0 0.0 0.003032554472259708 42 0.28809267486467227 0.0 0.0 0.0 0.003032554472259708 43 0.3062880016982305 0.0 0.0 0.0 0.003032554472259708 44 0.32751588300404844 0.0 0.0 0.0 0.003032554472259708 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATTGA 20 3.9100597E-4 50.618355 40 CTACAAA 15 0.007997288 50.61835 40 CATTGAA 60 0.0 50.579353 41 AATTATA 35 4.7364665E-8 50.57935 41 TTGTCTA 15 0.0080462005 50.540417 37 GGCGCAT 65 0.0 50.540417 37 AATAACG 15 0.008095335 50.462723 36 ATTGCTT 20 4.000267E-4 50.385265 34 TAGAGCG 25 1.9739698E-5 50.385265 34 ATTATTA 15 0.008319209 50.11603 47 AACAATT 15 0.008344365 50.077805 43 GCCCAAG 20 4.1231076E-4 50.077805 48 CAACAAT 15 0.008344365 50.077805 42 CCCACAA 15 0.008344365 50.077805 14 ACATCCT 15 0.008344365 50.077805 14 TAATCCG 15 0.008344365 50.077805 42 CCCTCCT 20 4.1231076E-4 50.077805 14 AGATTAA 65 0.0 50.077805 49 TTGCCTA 20 4.1231076E-4 50.077805 42 ACAACAC 15 0.008344365 50.077805 43 >>END_MODULE