FastQCFastQC Report
Fri 17 Jun 2016
SRR1525849_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525849_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6836
Sequences flagged as poor quality0
Sequence length40
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC230.33645406670567585No Hit
AATAAAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC230.33645406670567585No Hit
AATAAAGGGATGAGATGGACTTAAATGAATTGACGGGTAC210.3071971913399649No Hit
AATAAAGGGATGAGATGGACTCAAATGAATTGACGGGTAC210.3071971913399649No Hit
AATAAAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC210.3071971913399649No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.27794031597425395No Hit
AATAAAGGGAATTGACGGGTACGCACAGTTCTGAGTCGGA170.248683440608543No Hit
AATAAAGGGCTCGTCTTAATCAGCAACATCTTTTGATACA120.17554125219426564No Hit
AATAAAGGGATGAGATGGCCTTAAATGAATTGACGGGTAC120.17554125219426564No Hit
AATAAAGGGATTGACGGGTACGCACCGTTCTGAGTCGGAG110.16091281451141018No Hit
AATAAAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT110.16091281451141018No Hit
AATAAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT110.16091281451141018No Hit
AATAAAGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC110.16091281451141018No Hit
AATAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG110.16091281451141018No Hit
AATAAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT100.14628437682855472No Hit
AATAAAGGGATGAGATGGCCTCAAATGAATTGACGGGTAC100.14628437682855472No Hit
AATAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC100.14628437682855472No Hit
AATAAAGGGATGAGATGGACTTACAGGAATTGACGGGTAC100.14628437682855472No Hit
AATAAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT90.13165593914569923No Hit
AATAAAGGGATTGACGGGTACGCACAGTTCTGAGTCGGAG80.11702750146284377No Hit
AATAAAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCCGA70.10239906377998831No Hit
AATAAAGGGGCCGAATAGCCTCTCCACCCCAGCGGCCGGA70.10239906377998831No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGAATT251.6997987E-434.40298525
AAGGAAT200.002450808234.40298524
GGATGAG301.3061583E-533.897068
AGGGCTG200.002638400833.897066
AAGGGTT200.002638400833.897065
AAGGGTG406.41885E-833.897065
AAGGGTC200.002638400833.897065
AAGGGGT200.002638400833.897065
AAGGGCT454.478352E-933.897065
AAGGGAT900.033.897065
AGGGATG454.478352E-933.897066
GATGAGA301.3061583E-533.897069
ATGGACT200.002638400833.8970615
AGGGAAC359.168198E-733.897066
AAAGGGT950.033.897064
GGGATGA301.3061583E-533.897067
ATGAGAT301.3061583E-533.8970610
AGGGGAG251.8575105E-433.8970576
AGGGTGG251.8575105E-433.8970576
AGGGATT251.8575105E-433.8970576
Produced by FastQC (version 0.11.5)