##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525849_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6836
Sequences flagged as poor quality	0
Sequence length	40
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.220889409011118	23.0	14.0	29.0	4.0	33.0
2	18.610737273259215	21.0	10.0	26.0	4.0	31.0
3	14.5040959625512	15.0	10.0	15.0	4.0	33.0
4	18.613224107665303	20.0	6.0	27.0	4.0	33.0
5	17.98610298420129	20.0	6.0	26.0	4.0	31.0
6	15.34318314803979	15.0	4.0	21.0	4.0	27.0
7	15.06173200702165	16.0	4.0	22.0	4.0	27.0
8	19.475277940315973	23.0	4.0	29.0	4.0	32.0
9	17.398332358104156	19.0	4.0	27.0	4.0	31.0
10	16.046225863077822	17.0	4.0	24.0	4.0	30.0
11	21.328262141603275	24.0	4.0	33.0	4.0	36.0
12	20.286863662960794	22.0	4.0	33.0	4.0	36.0
13	21.010239906378	24.0	4.0	33.0	4.0	36.0
14	21.16998244587478	25.0	4.0	33.0	4.0	36.0
15	20.03715623171445	22.0	4.0	33.0	4.0	36.0
16	18.06772966647162	18.0	4.0	30.0	4.0	35.0
17	18.365272088940902	20.0	4.0	31.0	4.0	35.0
18	18.312317144528965	20.0	4.0	31.0	4.0	35.0
19	16.493270918665885	15.0	4.0	28.0	4.0	34.0
20	16.96255119953189	15.0	4.0	30.0	4.0	34.0
21	16.06977764774722	12.0	4.0	29.0	4.0	34.0
22	15.745172615564657	10.0	4.0	29.0	4.0	33.0
23	16.035985956699825	11.0	4.0	29.0	4.0	34.0
24	15.292276184903452	4.0	4.0	29.0	4.0	33.0
25	14.639847864248098	4.0	4.0	28.0	4.0	33.0
26	13.403159742539497	4.0	4.0	26.0	4.0	33.0
27	13.320509069631363	4.0	4.0	26.0	4.0	34.0
28	12.999707431246343	4.0	4.0	25.0	4.0	33.0
29	12.752779403159742	4.0	4.0	26.0	4.0	33.0
30	12.165740198946752	4.0	4.0	24.0	4.0	33.0
31	11.454944411936806	4.0	4.0	21.0	4.0	32.0
32	10.852984201287303	4.0	4.0	17.0	4.0	32.0
33	10.18929198361615	4.0	4.0	15.0	4.0	31.0
34	9.4969280280866	4.0	4.0	10.0	4.0	30.0
35	9.126974839087186	4.0	4.0	4.0	4.0	29.0
36	8.918519602106494	4.0	4.0	4.0	4.0	30.0
37	8.641018139262727	4.0	4.0	4.0	4.0	28.0
38	8.427735517846694	4.0	4.0	4.0	4.0	28.0
39	8.317583382094792	4.0	4.0	4.0	4.0	27.0
40	8.346840257460503	4.0	4.0	4.0	4.0	28.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	13.469177906043473
1	2	14.692334676685824
1	3	19.605850038874664
1	4	14.980969313147668
1	5	13.839299772370978
1	6	12.798442207009208
1	7	12.23256930033882
1	8	8.050812170551524
1	9	5.259062997167478
1	10	12.062159195679603
1	11	1.9109986586173306
1	12	2.20403240405458
1	13	-4.17025330739949
1	14	2.108368371744593
1	15	-0.7766405309173727
1	16	0.7924782232457446
1	17	1.2740834823266027
1	18	-1.314145007609957
1	19	-2.8896098009995566
1	20	2.480882665717404
1	21	5.0554766359047605
1	22	5.153387675373029
1	23	4.798024658965213
1	24	2.885800289624603
1	25	6.202283541288644
1	26	3.526057905911502
1	27	2.0658942114033056
1	28	3.6047552709786483
1	29	3.3978677119981473
1	30	4.068536898623414
1	31	4.121518736735023
1	32	3.6280619704410757
1	33	3.079590650771882
1	34	2.651651590793117
1	35	1.8482249197366762
1	36	1.6569424929551388
1	37	5.220324330973179
1	38	3.511264646449874
1	39	5.54709401647844
1	40	4.0205650672653785
2	1	15.397749334614904
2	2	16.323287057638204
2	3	17.808230991255613
2	4	16.897635979814332
2	5	17.494061677132887
2	6	-9.80870065013365
2	7	-9.731716413946895
2	8	-13.306330686591334
2	9	-11.84807985997538
2	10	-10.116412232891824
2	11	-15.017572769954096
2	12	-13.831681881659705
2	13	-14.99168187882806
2	14	-15.248774485398265
2	15	-14.098069102345946
2	16	-12.707521776754255
2	17	-12.61877366053054
2	18	-12.635573579038528
2	19	-10.889609800999557
2	20	-11.376260191425454
2	21	-10.623094792666667
2	22	-10.382326610341256
2	23	-10.27340391246336
2	24	-10.00705685323254
2	25	-8.940573601568499
2	26	-8.473942094088498
2	27	-8.112677217168123
2	28	-7.716673300449923
2	29	-7.387846573716139
2	30	-6.931463101376586
2	31	-6.307052691836406
2	32	-5.800509458130353
2	33	-4.884695063513831
2	34	-5.026919837778312
2	35	-4.758917937406181
2	36	-4.593057507044861
2	37	-4.101104240455392
2	38	-4.024449639264413
2	39	-3.667191697807273
2	40	-4.015149218448908
3	1	16.897749334614904
3	2	19.15662039097154
3	3	22.64156432458895
3	4	19.730969313147668
3	5	19.16072834379955
3	6	16.691299349866348
3	7	-9.231716413946895
3	8	-2.806330686591334
3	9	2.65192014002462
3	10	0.38358776710817644
3	11	-10.017572769954096
3	12	-0.8316818816597049
3	13	-11.99168187882806
3	14	-4.248774485398265
3	15	0.4019308976540543
3	16	-12.707521776754255
3	17	-12.61877366053054
3	18	-12.635573579038528
3	19	-10.889609800999557
3	20	-11.376260191425454
3	21	-10.623094792666667
3	22	-10.382326610341256
3	23	-10.27340391246336
3	24	-10.00705685323254
3	25	-8.940573601568499
3	26	-8.473942094088498
3	27	-8.112677217168123
3	28	-7.716673300449923
3	29	-7.387846573716139
3	30	-6.931463101376586
3	31	-6.307052691836406
3	32	-5.800509458130353
3	33	-4.884695063513831
3	34	-5.026919837778312
3	35	-4.758917937406181
3	36	-4.593057507044861
3	37	-4.101104240455392
3	38	-4.024449639264413
3	39	-3.667191697807273
3	40	-4.015149218448908
4	1	14.397749334614904
4	2	2.15662039097154
4	3	5.641564324588947
4	4	2.7309693131476678
4	5	2.6607283437995513
4	6	-9.80870065013365
4	7	-9.731716413946895
4	8	-13.306330686591334
4	9	-11.84807985997538
4	10	-10.116412232891824
4	11	-15.017572769954096
4	12	-13.831681881659705
4	13	-14.99168187882806
4	14	-15.248774485398265
4	15	-14.098069102345946
4	16	-12.707521776754255
4	17	-12.61877366053054
4	18	-12.635573579038528
4	19	-10.889609800999557
4	20	-11.376260191425454
4	21	-10.623094792666667
4	22	-10.382326610341256
4	23	-10.27340391246336
4	24	-10.00705685323254
4	25	-8.940573601568499
4	26	-8.473942094088498
4	27	-8.112677217168123
4	28	-7.716673300449923
4	29	-7.387846573716139
4	30	-6.931463101376586
4	31	-6.307052691836406
4	32	-5.800509458130353
4	33	-4.884695063513831
4	34	-5.026919837778312
4	35	-4.758917937406181
4	36	-4.593057507044861
4	37	-4.101104240455392
4	38	-4.024449639264413
4	39	-3.667191697807273
4	40	-4.015149218448908
6	1	-3.602250665385096
6	2	-4.676712942361794
6	3	1.1415643245889466
6	4	-2.4356973535189983
6	5	-2.8392716562004487
6	6	-4.475367316800316
6	7	-3.065049747280229
6	8	-2.306330686591334
6	9	-4.514746526642046
6	10	-3.7830788995584896
6	11	-4.35090610328743
6	12	-5.498348548326371
6	13	-6.325015212161395
6	14	-0.5821078187315969
6	15	4.901930897654054
6	16	-1.0408551100875894
6	17	-0.28544032719720747
6	18	-0.6355735790385282
6	19	-1.5562764676662226
6	20	2.9570731419078786
6	21	6.376905207333333
6	22	4.617673389658744
6	23	4.72659608753664
6	24	4.992943146767461
6	25	5.726093065098169
6	26	2.859391239244836
6	27	7.220656116165209
6	28	-1.383339967116589
6	29	1.6121534262838608
6	30	3.73520356529008
6	31	5.026280641496928
6	32	3.866157208536313
6	33	5.781971603152835
6	34	6.306413495555022
6	35	6.241082062593819
6	36	3.073609159621805
6	37	-2.101104240455392
6	38	-2.0244496392644127
6	39	3.999474968859393
6	40	4.651517448217758
7	1	2.1977493346149046
7	2	4.389953724304874
7	3	1.541564324588947
7	4	8.364302646481
7	5	2.7607283437995527
7	6	4.991299349866351
7	7	1.268283586053105
7	8	0.227002646742001
7	9	0.9519201400246207
7	10	5.483587767108178
7	11	6.915760563379237
7	12	4.501651451673627
7	13	10.941651454505273
7	14	8.684558847935069
7	15	10.36859756432072
7	16	7.225811556579078
7	17	10.314559672802794
7	18	8.231093087628139
7	19	9.443723532333776
7	20	10.157073141907881
7	21	13.776905207333332
7	22	13.08434005632541
7	23	10.393262754203308
7	24	10.19294314676746
7	25	9.992759731764835
7	26	11.259391239244836
7	27	8.220656116165209
7	28	9.149993366216744
7	29	11.545486759617194
7	30	9.668536898623415
7	31	2.159613974830261
7	32	4.599490541869647
7	33	1.9819716031528358
7	34	1.5730801622216877
7	35	2.0410820625938193
7	36	1.6069424929551381
7	37	2.2988957595446085
7	38	-1.6911163059310796
7	39	-0.13385836447393995
7	40	-0.34848255178224097
9	1	3.731082667948236
9	2	-4.010046275695126
9	3	1.1415643245889466
9	4	7.564302646481
9	5	2.827395010466219
9	6	3.524632683199682
9	7	8.268283586053105
9	8	9.027002646741998
9	9	7.485253473357952
9	10	12.216921100441509
9	11	17.982427230045904
9	12	16.83498478500696
9	13	17.00831812117194
9	14	14.751225514601735
9	15	15.23526423098739
9	16	13.625811556579077
9	17	20.38122633946946
9	18	19.697759754294808
9	19	15.777056865667111
9	20	20.62373980857455
9	21	4.376905207333333
9	22	8.951006722992076
9	23	10.059929420869972
9	24	9.326276480100793
9	25	12.059426398431501
9	26	6.19272457257817
9	27	8.887322782831877
9	28	3.9499933662167432
9	29	2.278820092950527
9	30	7.735203565290082
9	31	9.359613974830262
9	32	11.866157208536315
9	33	5.781971603152835
9	34	9.30641349555502
9	35	10.57441539592715
9	36	15.073609159621807
9	37	14.565562426211276
9	38	11.30888369406892
9	39	8.66614163552606
9	40	8.984850781551092
10	1	10.702097160701861
10	2	6.3087943040150165
10	3	19.576346933284597
10	4	16.665751921843317
10	5	10.073771822060422
10	6	12.713038480301131
10	7	15.05089228170528
10	8	15.693669313408666
10	9	16.934528835676794
10	10	14.753152984499481
10	11	10.63460114308938
10	12	12.646578987905514
10	13	10.095274642911068
10	14	11.88166029721043
10	15	7.206278723741011
10	16	9.857695614550092
10	17	11.729052426425984
10	18	12.016600334004949
10	19	14.110390199000443
10	20	12.145478939009328
10	21	14.463861729072462
10	22	17.095934259223963
10	23	16.16137869623229
10	24	14.514682277202242
10	25	15.88551335495324
10	26	7.613014427650631
10	27	6.800366261092748
10	28	7.457239743028339
10	29	8.873022991501252
10	30	2.6337542899277615
10	31	5.345121221207073
10	32	4.242968802739211
10	33	4.071826675616604
10	34	4.712210597004297
10	35	5.241082062593819
10	36	2.015638145129051
10	37	1.5510696725880866
10	38	0.8885938389964565
10	39	3.8110691717579437
10	40	1.3326768685076136
12	1	3.897749334614904
12	2	0.6566203909715398
12	3	1.1415643245889466
12	4	-2.7690306868523322
12	5	3.6607283437995513
12	6	6.69129934986635
12	7	0.768283586053105
12	8	10.193669313408666
12	9	4.65192014002462
12	10	10.383587767108176
12	11	17.982427230045904
12	12	9.168318118340295
12	13	15.50831812117194
12	14	14.251225514601735
12	15	4.401930897654054
12	16	9.292478223245745
12	17	16.88122633946946
12	18	8.864426420961472
12	19	10.110390199000443
12	20	16.12373980857455
12	21	18.876905207333333
12	22	19.117673389658744
12	23	19.22659608753664
12	24	12.99294314676746
12	25	14.059426398431501
12	26	5.026057905911502
12	27	8.387322782831877
12	28	9.783326699550077
12	29	-0.8878465737161392
12	30	-0.43146310137658617
12	31	7.692947308163594
12	32	11.199490541869647
12	33	1.6153049364861687
12	34	7.973080162221688
12	35	12.241082062593819
12	36	0.9069424929551388
12	37	1.398895759544608
12	38	1.4755503607355873
12	39	13.332808302192728
12	40	1.484850781551092
13	1	0.8977493346149039
13	2	5.65662039097154
13	3	-0.8584356754110534
13	4	-4.394030686852332
13	5	5.035728343799551
13	6	1.69129934986635
13	7	-0.356716413946895
13	8	1.818669313408666
13	9	2.15192014002462
13	10	-1.2414122328918236
13	11	7.732427230045904
13	12	4.418318118340295
13	13	6.133318121171939
13	14	3.6262255146017353
13	15	2.1519308976540543
13	16	10.917478223245745
13	17	9.50622633946946
13	18	9.989426420961472
13	19	10.860390199000443
13	20	11.123739808574546
13	21	8.251905207333333
13	22	12.367673389658744
13	23	15.47659608753664
13	24	6.492943146767461
13	25	11.809426398431501
13	26	1.026057905911502
13	27	2.262322782831877
13	28	1.6583266995500772
13	29	0.3621534262838608
13	30	4.068536898623414
13	31	-1.5570526918364056
13	32	0.4494905418696469
13	33	-1.2596950635138313
13	34	-5.026919837778312
13	35	-4.758917937406181
13	36	-4.593057507044861
13	37	-4.101104240455392
13	38	-4.024449639264413
13	39	-3.667191697807273
13	40	-4.015149218448908
14	1	-15.602250665385096
14	2	-13.34337960902846
14	3	-9.858435675411053
14	4	-12.769030686852332
14	5	-12.839271656200449
14	6	-9.80870065013365
14	7	-9.731716413946895
14	8	-13.306330686591334
14	9	-11.84807985997538
14	10	-10.116412232891824
14	11	-15.017572769954096
14	12	-13.831681881659705
14	13	-14.99168187882806
14	14	-15.248774485398265
14	15	-14.098069102345946
14	16	-12.707521776754255
14	17	-12.61877366053054
14	18	-12.635573579038528
14	19	-10.889609800999557
14	20	-11.376260191425454
14	21	-10.623094792666667
14	22	-10.382326610341256
14	23	-10.27340391246336
14	24	-10.00705685323254
14	25	-8.940573601568499
14	26	-8.473942094088498
14	27	-8.112677217168123
14	28	-7.716673300449923
14	29	-7.387846573716139
14	30	-6.931463101376586
14	31	-6.307052691836406
14	32	-5.800509458130353
14	33	-4.884695063513831
14	34	-5.026919837778312
14	35	-4.758917937406181
14	36	-4.593057507044861
14	37	-4.101104240455392
14	38	-4.024449639264413
14	39	-3.667191697807273
14	40	-4.015149218448908
15	1	-15.602250665385096
15	2	-13.34337960902846
15	3	-9.858435675411053
15	4	-12.769030686852332
15	5	-12.839271656200449
15	6	-9.80870065013365
15	7	-9.731716413946895
15	8	-13.306330686591334
15	9	-11.84807985997538
15	10	-10.116412232891824
15	11	-15.017572769954096
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112	19	1.510390199000442
112	20	1.6237398085745465
112	21	-4.423094792666667
112	22	3.6176733896587443
112	23	6.326596087536641
112	24	6.19294314676746
112	25	0.059426398431501326
112	26	-2.473942094088498
112	27	-3.112677217168123
112	28	-2.5166733004499235
112	29	-2.18784657371614
112	30	-2.931463101376586
112	31	-1.3070526918364056
112	32	-2.4005094581303528
112	33	0.315304936486168
112	34	0.17308016222168732
112	35	-0.35891793740618105
112	36	-1.1930575070448608
112	37	1.0988957595446074
112	38	0.575550360735587
112	39	0.33280830219272683
112	40	-0.01514921844890793
114	1	-15.602250665385096
114	2	-13.34337960902846
114	3	-9.858435675411053
114	4	-12.769030686852332
114	5	-12.839271656200449
114	6	-9.80870065013365
114	7	-9.731716413946895
114	8	-13.306330686591334
114	9	-11.84807985997538
114	10	-10.116412232891824
114	11	-15.017572769954096
114	12	-13.831681881659705
114	13	-14.99168187882806
114	14	-15.248774485398265
114	15	-14.098069102345946
114	16	-12.707521776754255
114	17	-12.61877366053054
114	18	-12.635573579038528
114	19	-10.889609800999557
114	20	-11.376260191425454
114	21	-10.623094792666667
114	22	-10.382326610341256
114	23	-10.27340391246336
114	24	-10.00705685323254
114	25	-8.940573601568499
114	26	-8.473942094088498
114	27	-8.112677217168123
114	28	-7.716673300449923
114	29	-7.387846573716139
114	30	-6.931463101376586
114	31	-6.307052691836406
114	32	-5.800509458130353
114	33	-4.884695063513831
114	34	-5.026919837778312
114	35	-4.758917937406181
114	36	-4.593057507044861
114	37	-4.101104240455392
114	38	-4.024449639264413
114	39	-3.667191697807273
114	40	-4.015149218448908
117	1	13.397749334614904
117	2	15.65662039097154
117	3	14.141564324588947
117	4	-6.769030686852332
117	5	-5.839271656200449
117	6	10.19129934986635
117	7	9.268283586053105
117	8	8.693669313408666
117	9	-7.84807985997538
117	10	4.883587767108176
117	11	10.982427230045904
117	12	8.168318118340295
117	13	7.008318121171939
117	14	10.751225514601735
117	15	4.901930897654054
117	16	4.292478223245745
117	17	11.38122633946946
117	18	-5.635573579038528
117	19	18.11039019900044
117	20	-11.376260191425454
117	21	-10.623094792666667
117	22	-10.382326610341256
117	23	-10.27340391246336
117	24	-10.00705685323254
117	25	-8.940573601568499
117	26	-8.473942094088498
117	27	-8.112677217168123
117	28	-7.716673300449923
117	29	-7.387846573716139
117	30	-6.931463101376586
117	31	-6.307052691836406
117	32	-5.800509458130353
117	33	-4.884695063513831
117	34	-5.026919837778312
117	35	-4.758917937406181
117	36	-4.593057507044861
117	37	-4.101104240455392
117	38	-4.024449639264413
117	39	-3.667191697807273
117	40	-4.015149218448908
118	1	-2.102250665385096
118	2	-0.8433796090284602
118	3	0.14156432458894663
118	4	-5.769030686852332
118	5	-4.089271656200449
118	6	-4.30870065013365
118	7	-1.981716413946895
118	8	-3.806330686591334
118	9	-1.59807985997538
118	10	-4.116412232891824
118	11	-3.2675727699540964
118	12	-3.331681881659705
118	13	-2.991681878828061
118	14	-5.498774485398265
118	15	-3.3480691023459457
118	16	-2.7075217767542554
118	17	-1.1187736605305396
118	18	1.6144264209614718
118	19	-7.1396098009995566
118	20	-3.6262601914254535
118	21	0.37690520733333344
118	22	-1.8823266103412557
118	23	-1.02340391246336
118	24	-3.5070568532325392
118	25	-8.940573601568499
118	26	-8.473942094088498
118	27	-8.112677217168123
118	28	-7.716673300449923
118	29	-7.387846573716139
118	30	-6.931463101376586
118	31	-6.307052691836406
118	32	-5.800509458130353
118	33	-4.884695063513831
118	34	-5.026919837778312
118	35	-4.758917937406181
118	36	-4.593057507044861
118	37	-4.101104240455392
118	38	-4.024449639264413
118	39	-3.667191697807273
118	40	-4.015149218448908
119	1	7.397749334614904
119	2	6.15662039097154
119	3	13.891564324588947
119	4	10.980969313147668
119	5	5.910728343799551
119	6	-0.30870065013365
119	7	1.268283586053105
119	8	2.443669313408666
119	9	-1.09807985997538
119	10	2.8835877671081764
119	11	-4.517572769954096
119	12	-1.331681881659705
119	13	-0.9916818788280608
119	14	-4.248774485398265
119	15	-3.5980691023459457
119	16	2.0424782232457446
119	17	-5.36877366053054
119	18	-7.885573579038528
119	19	-6.1396098009995566
119	20	-4.1262601914254535
119	21	-10.623094792666667
119	22	-10.382326610341256
119	23	-10.27340391246336
119	24	-10.00705685323254
119	25	-8.940573601568499
119	26	-8.473942094088498
119	27	-8.112677217168123
119	28	-7.716673300449923
119	29	-7.387846573716139
119	30	-6.931463101376586
119	31	-6.307052691836406
119	32	-5.800509458130353
119	33	-4.884695063513831
119	34	-5.026919837778312
119	35	-4.758917937406181
119	36	-4.593057507044861
119	37	-4.101104240455392
119	38	-4.024449639264413
119	39	-3.667191697807273
119	40	-4.015149218448908
120	1	13.597749334614907
120	2	16.65662039097154
120	3	19.74156432458895
120	4	17.030969313147665
120	5	15.960728343799548
120	6	3.9912993498663507
120	7	0.268283586053105
120	8	2.0936693134086646
120	9	5.751920140024621
120	10	0.08358776710817573
120	11	-0.6175727699540978
120	12	-5.431681881659705
120	13	1.80831812117194
120	14	1.9512255146017345
120	15	-2.6980691023459453
120	16	-7.707521776754255
120	17	-9.818773660530539
120	18	-1.435573579038529
120	19	-5.089609800999556
120	20	-11.376260191425454
120	21	-10.623094792666667
120	22	-10.382326610341256
120	23	-10.27340391246336
120	24	-10.00705685323254
120	25	-8.940573601568499
120	26	-8.473942094088498
120	27	-8.112677217168123
120	28	-7.716673300449923
120	29	-7.387846573716139
120	30	-6.931463101376586
120	31	-6.307052691836406
120	32	-5.800509458130353
120	33	-4.884695063513831
120	34	-5.026919837778312
120	35	-4.758917937406181
120	36	-4.593057507044861
120	37	-4.101104240455392
120	38	-4.024449639264413
120	39	-3.667191697807273
120	40	-4.015149218448908
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	1602.0
5	60.0
6	56.0
7	74.0
8	68.0
9	123.0
10	230.0
11	231.0
12	242.0
13	234.0
14	256.0
15	248.0
16	338.0
17	276.0
18	301.0
19	313.0
20	285.0
21	326.0
22	310.0
23	269.0
24	245.0
25	218.0
26	175.0
27	141.0
28	73.0
29	58.0
30	34.0
31	29.0
32	10.0
33	6.0
34	2.0
35	2.0
36	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	95.75029308323563	2.6963657678780772	1.2309495896834701	0.3223915592028136
8	95.40330844678671	2.7960767091201872	0.878348704435661	0.922266139657444
9	95.13838043637428	3.192268267681945	1.0103968370185972	0.658954458925172
10	31.445712613403572	42.932396839332746	11.691542288557214	13.930348258706468
11	25.3949678174371	32.080163838502045	26.331187829139846	16.19368051492101
12	39.877121123464015	19.133996489174958	20.787009947337623	20.201872440023404
13	26.887068461088354	26.272674078408425	23.68344060854301	23.156816851960212
14	28.788765359859568	30.4856641310708	22.030427150380337	18.695143358689293
15	23.244587478057344	31.612053832650673	24.502633118782914	20.64072557050907
16	24.897600936220012	21.898771211234642	30.16383850204798	23.039789350497365
17	33.27969572849619	26.36044470450556	19.748390871854888	20.61146869514336
18	33.16266822703335	25.48273844353423	19.54359274429491	21.811000585137506
19	19.13489736070381	24.04692082111437	18.533724340175954	38.28445747800587
20	21.079578700994734	28.32065535400819	20.93329432416618	29.666471620830897
21	18.92919836161498	23.829724985371563	30.236980690462257	27.0040959625512
22	19.824433065106074	23.643013899049013	25.866861741038772	30.665691294806148
23	23.58104154476302	29.959040374488005	24.956114686951434	21.503803393797543
24	18.434528163862474	29.64155084125823	22.970007315288953	28.953913679590343
25	19.795021961932648	31.976573938506586	22.518301610541727	25.710102489019032
26	19.457946678450437	26.263072617469437	28.811312417145384	25.467668286934746
27	23.719637108574773	23.7928007023705	27.611940298507463	24.875621890547265
28	16.439759918020787	30.551895769287075	30.361586883326012	22.646757429366122
29	15.596599237760186	28.437408384637937	32.233948988566404	23.732043389035475
30	15.140845070422534	20.583920187793428	37.573356807511736	26.7018779342723
31	15.6497506600176	24.361982986212965	41.19976532707539	18.788501026694046
32	25.062179956108267	20.907095830285297	33.430870519385515	20.599853694220922
33	16.57401989467525	27.662375658279696	35.152135751901696	20.61146869514336
34	15.696313633703921	23.624926857811584	38.31187829139848	22.366881217086014
35	20.34245572954778	24.176789111663986	37.2018147226694	18.278940436118834
36	17.298404800234156	25.230499048734085	36.82130835650519	20.64978779452656
37	17.677211482132396	23.50615114235501	37.03866432337434	21.777973052138254
38	14.996342355523042	25.032918800292613	40.307242136064374	19.663496708119972
39	14.689100219458668	29.436722750548643	35.62545720555962	20.248719824433064
40	13.66296079578701	27.047981275599764	35.576360444704505	23.71269748390872
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	70.0
1	54.0
2	38.0
3	38.0
4	24.0
5	10.0
6	7.0
7	4.0
8	4.0
9	12.0
10	20.0
11	31.0
12	42.0
13	42.0
14	48.5
15	55.0
16	76.5
17	98.0
18	98.0
19	115.5
20	133.0
21	148.5
22	164.0
23	164.0
24	186.0
25	208.0
26	229.0
27	250.0
28	250.0
29	307.0
30	364.0
31	381.0
32	398.0
33	398.0
34	402.0
35	406.0
36	419.0
37	432.0
38	432.0
39	461.5
40	491.0
41	501.5
42	512.0
43	512.0
44	486.5
45	461.0
46	452.0
47	443.0
48	443.0
49	453.0
50	463.0
51	459.0
52	455.0
53	455.0
54	419.0
55	383.0
56	355.0
57	327.0
58	327.0
59	282.5
60	238.0
61	191.5
62	145.0
63	145.0
64	126.5
65	108.0
66	81.0
67	54.0
68	54.0
69	39.0
70	24.0
71	24.5
72	25.0
73	25.0
74	18.0
75	11.0
76	6.0
77	1.0
78	1.0
79	1.5
80	2.0
81	1.5
82	1.0
83	1.0
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.17554125219426564
8	0.07314218841427736
9	0.10239906377998831
10	0.029256875365710942
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.23405500292568754
20	0.0
21	0.0
22	0.014628437682855471
23	0.0
24	0.014628437682855471
25	0.08777062609713282
26	0.6875365710942072
27	0.029256875365710942
28	0.07314218841427736
29	0.20479812755997662
30	0.29256875365710944
31	0.26331187829139846
32	0.014628437682855471
33	0.0
34	0.0
35	0.04388531304856641
36	0.04388531304856641
37	0.11702750146284377
38	0.014628437682855471
39	0.014628437682855471
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	6836.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.296079578701
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.09037013299151	87.72674078408426
2	2.5957378625220318	4.7396138092451725
3	0.5608075628905624	1.5359859566998246
4	0.1922768787053357	0.7021650087770626
5	0.17625380547989103	0.804564072557051
6	0.03204614645088928	0.17554125219426564
7	0.03204614645088928	0.20479812755997662
8	0.01602307322544464	0.11702750146284377
9	0.01602307322544464	0.13165593914569923
>10	0.2884153180580035	3.861907548273844
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATAAAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC	23	0.33645406670567585	No Hit
AATAAAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC	23	0.33645406670567585	No Hit
AATAAAGGGATGAGATGGACTTAAATGAATTGACGGGTAC	21	0.3071971913399649	No Hit
AATAAAGGGATGAGATGGACTCAAATGAATTGACGGGTAC	21	0.3071971913399649	No Hit
AATAAAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC	21	0.3071971913399649	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19	0.27794031597425395	No Hit
AATAAAGGGAATTGACGGGTACGCACAGTTCTGAGTCGGA	17	0.248683440608543	No Hit
AATAAAGGGCTCGTCTTAATCAGCAACATCTTTTGATACA	12	0.17554125219426564	No Hit
AATAAAGGGATGAGATGGCCTTAAATGAATTGACGGGTAC	12	0.17554125219426564	No Hit
AATAAAGGGATTGACGGGTACGCACCGTTCTGAGTCGGAG	11	0.16091281451141018	No Hit
AATAAAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT	11	0.16091281451141018	No Hit
AATAAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	11	0.16091281451141018	No Hit
AATAAAGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC	11	0.16091281451141018	No Hit
AATAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG	11	0.16091281451141018	No Hit
AATAAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT	10	0.14628437682855472	No Hit
AATAAAGGGATGAGATGGCCTCAAATGAATTGACGGGTAC	10	0.14628437682855472	No Hit
AATAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	10	0.14628437682855472	No Hit
AATAAAGGGATGAGATGGACTTACAGGAATTGACGGGTAC	10	0.14628437682855472	No Hit
AATAAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT	9	0.13165593914569923	No Hit
AATAAAGGGATTGACGGGTACGCACAGTTCTGAGTCGGAG	8	0.11702750146284377	No Hit
AATAAAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCCGA	7	0.10239906377998831	No Hit
AATAAAGGGGCCGAATAGCCTCTCCACCCCAGCGGCCGGA	7	0.10239906377998831	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.014628437682855471	0.0	0.0	0.0	0.0
10	0.014628437682855471	0.0	0.0	0.0	0.0
11	0.014628437682855471	0.0	0.0	0.0	0.0
12	0.014628437682855471	0.0	0.0	0.0	0.0
13	0.014628437682855471	0.0	0.0	0.0	0.0
14	0.014628437682855471	0.0	0.0	0.0	0.0
15	0.014628437682855471	0.0	0.0	0.0	0.0
16	0.014628437682855471	0.0	0.0	0.0	0.0
17	0.014628437682855471	0.0	0.0	0.0	0.0
18	0.014628437682855471	0.0	0.0	0.0	0.0
19	0.014628437682855471	0.0	0.0	0.0	0.0
20	0.014628437682855471	0.0	0.0	0.0	0.0
21	0.014628437682855471	0.0	0.0	0.0	0.0
22	0.014628437682855471	0.0	0.0	0.0	0.0
23	0.014628437682855471	0.0	0.0	0.0	0.0
24	0.014628437682855471	0.0	0.0	0.0	0.0
25	0.014628437682855471	0.0	0.0	0.0	0.0
26	0.014628437682855471	0.0	0.0	0.0	0.0
27	0.014628437682855471	0.0	0.0	0.0	0.0
28	0.014628437682855471	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGAATT	25	1.6997987E-4	34.402985	25
AAGGAAT	20	0.0024508082	34.402985	24
GGATGAG	30	1.3061583E-5	33.89706	8
AGGGCTG	20	0.0026384008	33.89706	6
AAGGGTT	20	0.0026384008	33.89706	5
AAGGGTG	40	6.41885E-8	33.89706	5
AAGGGTC	20	0.0026384008	33.89706	5
AAGGGGT	20	0.0026384008	33.89706	5
AAGGGCT	45	4.478352E-9	33.89706	5
AAGGGAT	90	0.0	33.89706	5
AGGGATG	45	4.478352E-9	33.89706	6
GATGAGA	30	1.3061583E-5	33.89706	9
ATGGACT	20	0.0026384008	33.89706	15
AGGGAAC	35	9.168198E-7	33.89706	6
AAAGGGT	95	0.0	33.89706	4
GGGATGA	30	1.3061583E-5	33.89706	7
ATGAGAT	30	1.3061583E-5	33.89706	10
AGGGGAG	25	1.8575105E-4	33.897057	6
AGGGTGG	25	1.8575105E-4	33.897057	6
AGGGATT	25	1.8575105E-4	33.897057	6
>>END_MODULE