FastQCFastQC Report
Fri 17 Jun 2016
SRR1525847_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525847_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4911
Sequences flagged as poor quality0
Sequence length40
%GC21

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1803.665241295051924No Hit
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA981.995520260639381No Hit
AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.7737731622887396No Hit
AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.6515984524536754No Hit
AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.631236000814498No Hit
AATAAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG280.570148645896966No Hit
AATAAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT250.509061290979434No Hit
AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.2647118713093056No Hit
AATAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC120.2443494196701283No Hit
AATAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT110.22398696803095092No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAA90.1832620647525962No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAATATTTTTT90.1832620647525962No Hit
AATAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA80.16289961311341886No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAA70.1425371614742415No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAA70.1425371614742415No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAA70.1425371614742415No Hit
AATAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.12217470983506415No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT60.12217470983506415No Hit
AATAAAGGGGGAAAAAAACAAAAAAAAAAAAAAAAAAAAA60.12217470983506415No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTTTTTTT50.10181225819588678No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATATA50.10181225819588678No Hit
AATAAAGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT50.10181225819588678No Hit
AATAAAGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAA50.10181225819588678No Hit
AATAAAGGGGGAACAAAAAAAAAAAAAAAAAAAAAAAAAA50.10181225819588678No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTATTT50.10181225819588678No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAT50.10181225819588678No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGAA200.0024370234.2474222
GGGTAAA251.7807273E-433.897967
AGGGTAA251.7807273E-433.897966
AAGGGTA251.7807273E-433.897965
AAGGGGA301.2308534E-533.897965
AAGGGAA502.6557245E-1033.897965
AGGGAAA251.7807273E-433.897966
AGGGGTT200.002565472633.8979576
AGGGGAA200.002565472633.8979576
AAGGGGT405.7911166E-833.8979575
AAGGGAT200.002565472633.8979575
AAAGGGT453.936293E-933.8979574
AAAGGGA950.033.8979574
ATAAAGG3000.032.768032
TAAAGGG2850.032.7085573
AATAAAG3350.031.8742031
AAAGGGG1300.031.2904244
AAGGGGG402.4054552E-629.6607135
AGGGGGG305.192652E-428.24836
GGTAAAA305.192652E-428.24838
Produced by FastQC (version 0.11.5)