##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525847_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4911
Sequences flagged as poor quality	0
Sequence length	40
%GC	21
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.858277336591325	31.0	12.0	34.0	4.0	35.0
2	19.090002036245163	22.0	4.0	33.0	4.0	35.0
3	16.285277947464873	13.0	4.0	30.0	4.0	35.0
4	15.527591121971085	10.0	4.0	29.0	4.0	35.0
5	14.78069639584606	4.0	4.0	30.0	4.0	35.0
6	10.070454082671553	4.0	4.0	15.0	4.0	27.0
7	9.788026878436163	4.0	4.0	16.0	4.0	26.0
8	10.894318875992669	4.0	4.0	19.0	4.0	30.0
9	10.61983302789656	4.0	4.0	18.0	4.0	29.0
10	9.972714314803502	4.0	4.0	15.0	4.0	28.0
11	10.136835675015272	4.0	4.0	14.0	4.0	30.0
12	9.680309509264916	4.0	4.0	10.0	4.0	30.0
13	9.053349623294645	4.0	4.0	8.0	4.0	28.0
14	9.207086133170433	4.0	4.0	6.0	4.0	29.0
15	8.804113215231114	4.0	4.0	4.0	4.0	27.0
16	8.287925066177968	4.0	4.0	4.0	4.0	26.0
17	7.780900020362451	4.0	4.0	4.0	4.0	23.0
18	7.68193850539605	4.0	4.0	4.0	4.0	23.0
19	7.459173284463449	4.0	4.0	4.0	4.0	22.0
20	7.686825493789453	4.0	4.0	4.0	4.0	24.0
21	7.039910405212788	4.0	4.0	4.0	4.0	19.0
22	7.134392180818571	4.0	4.0	4.0	4.0	21.0
23	6.779474648747709	4.0	4.0	4.0	4.0	17.0
24	6.913052331500713	4.0	4.0	4.0	4.0	19.0
25	6.833231521075137	4.0	4.0	4.0	4.0	18.0
26	6.885766646304215	4.0	4.0	4.0	4.0	17.0
27	7.066992465892893	4.0	4.0	4.0	4.0	20.0
28	7.298309916513948	4.0	4.0	4.0	4.0	25.0
29	6.8658114436978215	4.0	4.0	4.0	4.0	18.0
30	6.72327428222358	4.0	4.0	4.0	4.0	16.0
31	7.1722663408674405	4.0	4.0	4.0	4.0	21.0
32	7.178985949908369	4.0	4.0	4.0	4.0	23.0
33	7.1260435756465075	4.0	4.0	4.0	4.0	22.0
34	7.133374058236612	4.0	4.0	4.0	4.0	23.0
35	7.07798818977805	4.0	4.0	4.0	4.0	22.0
36	7.024638566483405	4.0	4.0	4.0	4.0	21.0
37	6.879250661779678	4.0	4.0	4.0	4.0	17.0
38	6.844227244960293	4.0	4.0	4.0	4.0	17.0
39	6.744858480961108	4.0	4.0	4.0	4.0	15.0
40	6.717980044797393	4.0	4.0	4.0	4.0	15.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	0.3755658230658234
1	2	4.671987179487182
1	3	-3.7761338661338613
1	4	5.233522866022859
1	5	-2.673586413586417
1	6	-0.6172621822621824
1	7	-2.369581252081254
1	8	-0.4688736263736253
1	9	-1.068588078588082
1	10	-5.275335220335217
1	11	-5.642485014985013
1	12	-5.206884226884229
1	13	-4.425997335997337
1	14	-4.5543911643911645
1	15	-4.291823731823731
1	16	-3.9985875235875223
1	17	-3.1215653790653777
1	18	-3.2375660450660435
1	19	-3.4047097347097326
1	20	-3.389699189699189
1	21	-3.0950396825396824
1	22	-3.0184435009434996
1	23	-3.295621878121878
1	24	-2.6571572871572853
1	25	-3.063883616383615
1	26	-2.4581515706515713
1	27	-2.4801232101232102
1	28	-2.6238095238095216
1	29	-2.4794222444222456
1	30	-2.551061161061159
1	31	-2.7149225774225796
1	32	-2.5706351981351974
1	33	-2.713703241203242
1	34	-2.865659895659898
1	35	-2.8193450993450977
1	36	-2.627075979575979
1	37	-2.30027306027306
1	38	-2.2914968364968376
1	39	-2.29018342768343
1	40	-2.2289388389388414
2	1	7.375565823065823
2	2	-0.6280128205128186
2	3	6.223866133866139
2	4	-9.96647713397714
2	5	-6.673586413586417
2	6	-1.8172621822621817
2	7	2.230418747918746
2	8	3.231126373626374
2	9	-6.268588078588081
2	10	-0.7753352203352168
2	11	2.357514985014987
2	12	4.293115773115771
2	13	8.074002664002663
2	14	4.9456088356088355
2	15	3.7081762681762687
2	16	7.501412476412478
2	17	11.378434620934623
2	18	7.2624339549339565
2	19	8.595290265290267
2	20	8.11030081030081
2	21	10.404960317460318
2	22	1.4815564990565004
2	23	10.704378121878122
2	24	0.3428427128427147
2	25	10.936116383616385
2	26	-2.4581515706515713
2	27	-2.4801232101232102
2	28	-2.6238095238095216
2	29	-2.4794222444222456
2	30	-2.551061161061159
2	31	-2.7149225774225796
2	32	-2.5706351981351974
2	33	-2.713703241203242
2	34	-2.865659895659898
2	35	-2.8193450993450977
2	36	-2.627075979575979
2	37	-2.30027306027306
2	38	-2.2914968364968376
2	39	-2.29018342768343
2	40	-2.2289388389388414
3	1	-8.124434176934177
3	2	1.3719871794871814
3	3	4.723866133866139
3	4	0.03352286602286014
3	5	1.826413586413583
3	6	1.1827378177378183
3	7	1.7304187479187458
3	8	4.731126373626374
3	9	4.731411921411919
3	10	5.724664779664783
3	11	5.857514985014987
3	12	-1.7068842268842292
3	13	10.074002664002663
3	14	2.4456088356088355
3	15	4.708176268176269
3	16	9.501412476412478
3	17	2.3784346209346223
3	18	6.7624339549339565
3	19	7.595290265290267
3	20	-0.889699189699189
3	21	11.404960317460318
3	22	8.4815564990565
3	23	11.204378121878122
3	24	-2.6571572871572853
3	25	-3.063883616383615
3	26	-2.4581515706515713
3	27	-2.4801232101232102
3	28	-2.6238095238095216
3	29	-2.4794222444222456
3	30	-2.551061161061159
3	31	-2.7149225774225796
3	32	-2.5706351981351974
3	33	-2.713703241203242
3	34	-2.865659895659898
3	35	-2.8193450993450977
3	36	-2.627075979575979
3	37	-2.30027306027306
3	38	-2.2914968364968376
3	39	-2.29018342768343
3	40	-2.2289388389388414
4	1	-10.624434176934177
4	2	-5.128012820512819
4	3	-5.276133866133861
4	4	-2.96647713397714
4	5	-1.173586413586417
4	6	-2.8172621822621817
4	7	2.230418747918746
4	8	0.731126373626374
4	9	1.7314119214119188
4	10	-2.775335220335217
4	11	8.357514985014987
4	12	5.793115773115771
4	13	7.074002664002663
4	14	1.9456088356088355
4	15	10.708176268176269
4	16	13.001412476412478
4	17	12.378434620934623
4	18	11.262433954933957
4	19	13.595290265290267
4	20	13.61030081030081
4	21	12.904960317460318
4	22	13.4815564990565
4	23	10.704378121878122
4	24	13.842842712842714
4	25	12.936116383616385
4	26	5.541848429348429
4	27	9.51987678987679
4	28	2.8761904761904784
4	29	2.5205777555777544
4	30	5.448938838938841
4	31	3.2850774225774204
4	32	2.9293648018648026
4	33	0.786296758796758
4	34	2.634340104340102
4	35	6.180654900654902
4	36	12.372924020424021
4	37	12.19972693972694
4	38	6.2085031635031624
4	39	10.70981657231657
4	40	7.271061161061159
5	1	5.042232489732491
5	2	2.2053205128205136
5	3	14.223866133866139
5	4	-1.633143800643806
5	5	-0.6735864135864169
5	6	-4.150595515595515
5	7	-4.102914585414587
5	8	-0.6022069597069599
5	9	1.3980785880785849
5	10	-1.9420018870018838
5	11	1.690848318348321
5	12	-0.20688422688422925
5	13	-2.092664002664004
5	14	-2.5543911643911645
5	15	1.0415096015096026
5	16	0.6680791430791437
5	17	1.8784346209346223
5	18	1.4291006216006226
5	19	0.9286235986236013
5	20	0.610300810300811
5	21	-1.4283730158730155
5	22	2.3148898323898344
5	23	4.371044788544788
5	24	2.009509379509381
5	25	3.269449716949719
5	26	4.541848429348429
5	27	4.853210123210124
5	28	5.709523809523812
5	29	-0.8127555777555786
5	30	4.448938838938841
5	31	5.618410755910754
5	32	-0.5706351981351974
5	33	6.286296758796758
5	34	5.467673437673436
5	35	6.847321567321568
5	36	7.039590687090687
5	37	8.033060273060274
5	38	7.3751698301698285
5	39	8.043149905649905
5	40	8.104394494394493
6	1	-4.624434176934177
6	2	-0.6280128205128186
6	3	2.2238661338661387
6	4	4.03352286602286
6	5	5.326413586413583
6	6	6.682737817737818
6	7	3.230418747918746
6	8	-1.768873626373626
6	9	2.231411921411919
6	10	1.7246647796647832
6	11	1.857514985014987
6	12	4.293115773115771
6	13	-1.9259973359973372
6	14	-0.05439116439116454
6	15	-2.2918237318237313
6	16	-1.9985875235875223
6	17	-2.1215653790653777
6	18	2.7624339549339565
6	19	1.0952902652902674
6	20	2.110300810300811
6	21	4.404960317460318
6	22	11.4815564990565
6	23	9.704378121878122
6	24	11.842842712842714
6	25	9.936116383616385
6	26	-2.4581515706515713
6	27	-2.4801232101232102
6	28	-2.6238095238095216
6	29	-2.4794222444222456
6	30	-2.551061161061159
6	31	-2.7149225774225796
6	32	-2.5706351981351974
6	33	-2.713703241203242
6	34	-2.865659895659898
6	35	-2.8193450993450977
6	36	-2.627075979575979
6	37	-2.30027306027306
6	38	-2.2914968364968376
6	39	-2.29018342768343
6	40	-2.2289388389388414
7	1	-21.624434176934177
7	2	-16.12801282051282
7	3	-14.776133866133861
7	4	-12.96647713397714
7	5	-11.673586413586417
7	6	-5.817262182262182
7	7	-5.769581252081254
7	8	-6.268873626373626
7	9	-6.268588078588081
7	10	-5.275335220335217
7	11	-5.642485014985013
7	12	-5.206884226884229
7	13	-4.425997335997337
7	14	-4.5543911643911645
7	15	-4.291823731823731
7	16	-3.9985875235875223
7	17	-3.1215653790653777
7	18	-3.2375660450660435
7	19	-3.4047097347097326
7	20	-3.389699189699189
7	21	-3.0950396825396824
7	22	-3.0184435009434996
7	23	-3.295621878121878
7	24	-2.6571572871572853
7	25	-3.063883616383615
7	26	-2.4581515706515713
7	27	-2.4801232101232102
7	28	-2.6238095238095216
7	29	-2.4794222444222456
7	30	-2.551061161061159
7	31	-2.7149225774225796
7	32	-2.5706351981351974
7	33	-2.713703241203242
7	34	-2.865659895659898
7	35	-2.8193450993450977
7	36	-2.627075979575979
7	37	-2.30027306027306
7	38	-2.2914968364968376
7	39	-2.29018342768343
7	40	-2.2289388389388414
8	1	-21.624434176934177
8	2	-16.12801282051282
8	3	-14.776133866133861
8	4	-12.96647713397714
8	5	-11.673586413586417
8	6	-5.817262182262182
8	7	-5.769581252081254
8	8	-6.268873626373626
8	9	-6.268588078588081
8	10	-5.275335220335217
8	11	-5.642485014985013
8	12	-5.206884226884229
8	13	-4.425997335997337
8	14	-4.5543911643911645
8	15	-4.291823731823731
8	16	-3.9985875235875223
8	17	-3.1215653790653777
8	18	-3.2375660450660435
8	19	-3.4047097347097326
8	20	-3.389699189699189
8	21	-3.0950396825396824
8	22	-3.0184435009434996
8	23	-3.295621878121878
8	24	-2.6571572871572853
8	25	-3.063883616383615
8	26	-2.4581515706515713
8	27	-2.4801232101232102
8	28	-2.6238095238095216
8	29	-2.4794222444222456
8	30	-2.551061161061159
8	31	-2.7149225774225796
8	32	-2.5706351981351974
8	33	-2.713703241203242
8	34	-2.865659895659898
8	35	-2.8193450993450977
8	36	-2.627075979575979
8	37	-2.30027306027306
8	38	-2.2914968364968376
8	39	-2.29018342768343
8	40	-2.2289388389388414
9	1	9.375565823065823
9	2	14.871987179487181
9	3	11.223866133866139
9	4	18.03352286602286
9	5	17.326413586413583
9	6	-5.817262182262182
9	7	-5.769581252081254
9	8	-6.268873626373626
9	9	-6.268588078588081
9	10	-5.275335220335217
9	11	-5.642485014985013
9	12	-5.206884226884229
9	13	-4.425997335997337
9	14	-4.5543911643911645
9	15	-4.291823731823731
9	16	-3.9985875235875223
9	17	-3.1215653790653777
9	18	-3.2375660450660435
9	19	-3.4047097347097326
9	20	-3.389699189699189
9	21	-3.0950396825396824
9	22	-3.0184435009434996
9	23	-3.295621878121878
9	24	-2.6571572871572853
9	25	-3.063883616383615
9	26	-2.4581515706515713
9	27	-2.4801232101232102
9	28	-2.6238095238095216
9	29	-2.4794222444222456
9	30	-2.551061161061159
9	31	-2.7149225774225796
9	32	-2.5706351981351974
9	33	-2.713703241203242
9	34	-2.865659895659898
9	35	-2.8193450993450977
9	36	-2.627075979575979
9	37	-2.30027306027306
9	38	-2.2914968364968376
9	39	-2.29018342768343
9	40	-2.2289388389388414
10	1	-1.1244341769341766
10	2	2.3719871794871814
10	3	3.2238661338661387
10	4	8.53352286602286
10	5	11.826413586413583
10	6	13.182737817737818
10	7	12.730418747918746
10	8	9.731126373626374
10	9	4.731411921411919
10	10	17.724664779664785
10	11	15.357514985014987
10	12	22.793115773115773
10	13	14.074002664002663
10	14	9.945608835608835
10	15	23.208176268176267
10	16	15.501412476412478
10	17	8.378434620934623
10	18	7.2624339549339565
10	19	16.595290265290267
10	20	24.11030081030081
10	21	11.404960317460318
10	22	20.481556499056502
10	23	20.204378121878122
10	24	22.842842712842714
10	25	20.936116383616387
10	26	13.041848429348429
10	27	18.01987678987679
10	28	26.37619047619048
10	29	13.520577755577754
10	30	20.44893883893884
10	31	22.28507742257742
10	32	18.929364801864804
10	33	16.28629675879676
10	34	18.1343401043401
10	35	22.6806549006549
10	36	15.372924020424021
10	37	6.69972693972694
10	38	24.208503163503163
10	39	12.20981657231657
10	40	5.771061161061159
11	1	10.875565823065823
11	2	14.871987179487181
11	3	17.72386613386614
11	4	17.53352286602286
11	5	20.826413586413583
11	6	-5.817262182262182
11	7	-5.769581252081254
11	8	-6.268873626373626
11	9	-6.268588078588081
11	10	-5.275335220335217
11	11	-5.642485014985013
11	12	-5.206884226884229
11	13	-4.425997335997337
11	14	-4.5543911643911645
11	15	-4.291823731823731
11	16	-3.9985875235875223
11	17	-3.1215653790653777
11	18	-3.2375660450660435
11	19	-3.4047097347097326
11	20	-3.389699189699189
11	21	-3.0950396825396824
11	22	-3.0184435009434996
11	23	-3.295621878121878
11	24	-2.6571572871572853
11	25	-3.063883616383615
11	26	-2.4581515706515713
11	27	-2.4801232101232102
11	28	-2.6238095238095216
11	29	-2.4794222444222456
11	30	-2.551061161061159
11	31	-2.7149225774225796
11	32	-2.5706351981351974
11	33	-2.713703241203242
11	34	-2.865659895659898
11	35	-2.8193450993450977
11	36	-2.627075979575979
11	37	-2.30027306027306
11	38	-2.2914968364968376
11	39	-2.29018342768343
11	40	-2.2289388389388414
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115	5	5.326413586413583
115	6	-4.317262182262182
115	7	-1.7695812520812542
115	8	-5.768873626373626
115	9	-5.268588078588081
115	10	-0.4420018870018829
115	11	-0.4758183483183469
115	12	-0.8735508935508953
115	13	-2.592664002664004
115	14	-3.0543911643911645
115	15	-2.7918237318237313
115	16	-1.3319208569208554
115	17	1.3784346209346223
115	18	-0.5708993783993765
115	19	0.5952902652902674
115	20	0.9436341436341449
115	21	-3.0950396825396824
115	22	-3.0184435009434996
115	23	-3.295621878121878
115	24	-2.6571572871572853
115	25	-3.063883616383615
115	26	-2.4581515706515713
115	27	-2.4801232101232102
115	28	-2.6238095238095216
115	29	-2.4794222444222456
115	30	-2.551061161061159
115	31	-2.7149225774225796
115	32	-2.5706351981351974
115	33	-2.713703241203242
115	34	-2.865659895659898
115	35	-2.8193450993450977
115	36	-2.627075979575979
115	37	-2.30027306027306
115	38	-2.2914968364968376
115	39	-2.29018342768343
115	40	-2.2289388389388414
116	1	-5.491100843600844
116	2	-7.061346153846152
116	3	-12.44280053280053
116	4	-10.033143800643806
116	5	-9.273586413586417
116	6	-4.9505955155955155
116	7	-3.9695812520812543
116	8	-4.20220695970696
116	9	-3.868588078588081
116	10	-2.4086685536685506
116	11	-2.9091516816516796
116	12	-3.140217560217563
116	13	-3.2926640026640035
116	14	-2.0877244977244978
116	15	-3.4918237318237315
116	16	-2.0652541902541888
116	17	-1.988232045732044
116	18	-1.5042327117327101
116	19	-2.471376401376399
116	20	-1.4563658563658555
116	21	-1.028373015873016
116	22	-2.2184435009434997
116	23	-1.4956218781218782
116	24	-0.7238239538239517
116	25	-1.1305502830502814
116	26	1.4751817626817623
116	27	1.6532101232101226
116	28	-0.49047619047618785
116	29	1.8539110889110884
116	30	0.3156055056055074
116	31	1.4184107559107533
116	32	1.5626981351981355
116	33	1.4196300921300908
116	34	0.001006771006768048
116	35	0.11398823398823588
116	36	-1.4270759795759789
116	37	1.3663936063936069
116	38	1.3751698301698294
116	39	-0.4235167610167636
116	40	-0.3622721722721751
117	1	1.1755658230658241
117	2	6.071987179487181
117	3	4.623866133866137
117	4	-3.166477133977139
117	5	0.9264135864135845
117	6	2.1827378177378183
117	7	4.230418747918746
117	8	1.3311263736263736
117	9	0.9314119214119181
117	10	5.324664779664783
117	11	4.957514985014987
117	12	2.5931157731157715
117	13	0.5740026640026628
117	14	4.045608835608835
117	15	0.5081762681762694
117	16	1.8014124764124784
117	17	-3.1215653790653777
117	18	-3.2375660450660435
117	19	-3.4047097347097326
117	20	-3.389699189699189
117	21	-3.0950396825396824
117	22	-3.0184435009434996
117	23	-3.295621878121878
117	24	-2.6571572871572853
117	25	-3.063883616383615
117	26	-2.4581515706515713
117	27	-2.4801232101232102
117	28	-2.6238095238095216
117	29	-2.4794222444222456
117	30	-2.551061161061159
117	31	-2.7149225774225796
117	32	-2.5706351981351974
117	33	-2.713703241203242
117	34	-2.865659895659898
117	35	-2.8193450993450977
117	36	-2.627075979575979
117	37	-2.30027306027306
117	38	-2.2914968364968376
117	39	-2.29018342768343
117	40	-2.2289388389388414
118	1	8.375565823065823
118	2	-3.3780128205128186
118	3	-3.0261338661338613
118	4	-6.21647713397714
118	5	2.576413586413583
118	6	-5.817262182262182
118	7	-5.769581252081254
118	8	-6.268873626373626
118	9	-6.268588078588081
118	10	-5.275335220335217
118	11	-5.642485014985013
118	12	-5.206884226884229
118	13	-4.425997335997337
118	14	-4.5543911643911645
118	15	-4.291823731823731
118	16	-3.9985875235875223
118	17	-3.1215653790653777
118	18	-3.2375660450660435
118	19	-3.4047097347097326
118	20	-3.389699189699189
118	21	-3.0950396825396824
118	22	-3.0184435009434996
118	23	-3.295621878121878
118	24	-2.6571572871572853
118	25	-3.063883616383615
118	26	-2.4581515706515713
118	27	-2.4801232101232102
118	28	-2.6238095238095216
118	29	-2.4794222444222456
118	30	-2.551061161061159
118	31	-2.7149225774225796
118	32	-2.5706351981351974
118	33	-2.713703241203242
118	34	-2.865659895659898
118	35	-2.8193450993450977
118	36	-2.627075979575979
118	37	-2.30027306027306
118	38	-2.2914968364968376
118	39	-2.29018342768343
118	40	-2.2289388389388414
119	1	-1.9577675102675087
119	2	-1.4613461538461507
119	3	-6.442800532800527
119	4	2.3668561993561923
119	5	-2.006919746919751
119	6	-5.817262182262182
119	7	-5.769581252081254
119	8	-6.268873626373626
119	9	-6.268588078588081
119	10	-5.275335220335217
119	11	-5.642485014985013
119	12	-5.206884226884229
119	13	-4.425997335997337
119	14	-4.5543911643911645
119	15	-4.291823731823731
119	16	-3.9985875235875223
119	17	-3.1215653790653777
119	18	-3.2375660450660435
119	19	-3.4047097347097326
119	20	-3.389699189699189
119	21	-3.0950396825396824
119	22	-3.0184435009434996
119	23	-3.295621878121878
119	24	-2.6571572871572853
119	25	-3.063883616383615
119	26	-2.4581515706515713
119	27	-2.4801232101232102
119	28	-2.6238095238095216
119	29	-2.4794222444222456
119	30	-2.551061161061159
119	31	-2.7149225774225796
119	32	-2.5706351981351974
119	33	-2.713703241203242
119	34	-2.865659895659898
119	35	-2.8193450993450977
119	36	-2.627075979575979
119	37	-2.30027306027306
119	38	-2.2914968364968376
119	39	-2.29018342768343
119	40	-2.2289388389388414
120	1	3.2922324897324913
120	2	5.788653846153849
120	3	-0.6928005328005291
120	4	4.950189532689528
120	5	6.576413586413583
120	6	8.182737817737818
120	7	0.3970854145854119
120	8	0.981126373626374
120	9	1.7314119214119188
120	10	0.47466477966478315
120	11	2.440848318348321
120	12	5.626449106449105
120	13	3.0740026640026628
120	14	5.278942168942169
120	15	4.708176268176269
120	16	3.9180791430791437
120	17	4.795101287601288
120	18	7.09576728826729
120	19	6.178623598623601
120	20	7.110300810300811
120	21	-0.011706349206349387
120	22	0.06488983238983348
120	23	-0.462288544788545
120	24	-1.9904906204906183
120	25	-1.397216949716948
120	26	-0.04148490398490434
120	27	-1.3967898767898772
120	28	-1.9571428571428546
120	29	-0.3127555777555786
120	30	-1.5510611610611589
120	31	-0.7149225774225796
120	32	-0.7373018648018643
120	33	-0.463703241203242
120	34	-0.6156598956598982
120	35	-0.6526784326784307
120	36	-0.37707597957597905
120	37	-0.5502730602730601
120	38	-0.12483016983017059
120	39	0.1264832389832371
120	40	-0.47893883893884137
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	1841.0
5	454.0
6	421.0
7	402.0
8	166.0
9	159.0
10	149.0
11	174.0
12	109.0
13	96.0
14	95.0
15	64.0
16	45.0
17	45.0
18	37.0
19	54.0
20	84.0
21	92.0
22	80.0
23	93.0
24	69.0
25	62.0
26	59.0
27	20.0
28	17.0
29	9.0
30	6.0
31	5.0
32	2.0
33	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	59.38837920489297	31.07033639143731	5.708460754332314	3.832823649337411
8	57.702509691899614	32.56478269740869	6.44766374209345	3.2850438685982453
9	58.73113015095879	32.45614035087719	5.793553651570788	3.0191758465932272
10	29.051987767584098	53.23139653414882	12.436289500509684	5.28032619775739
11	20.460566537599348	59.09924597513756	10.63786427552476	9.802323211738333
12	16.109979633401224	64.0122199592668	11.507128309572302	8.370672097759673
13	9.494702526487368	64.60880195599023	13.121434392828036	12.775061124694375
14	7.176350662589194	70.39755351681957	12.089704383282365	10.336391437308869
15	13.846467114640603	65.81144369782122	13.683567501527182	6.658521686010996
16	9.794339238444309	69.23233557320302	13.622480146609654	7.350845041743026
17	9.305640399104051	65.09875789045	16.24923640806353	9.346365302382406
18	9.407452657299938	67.84768886173896	13.683567501527182	9.061290979433924
19	7.065770718794543	68.84544899205865	12.461820403176542	11.62695988597027
20	8.959478721238037	66.97210344125433	15.292201181022195	8.77621665648544
21	6.943596008959478	69.00834860517206	12.706169822846672	11.341885563021787
22	7.676644267969864	60.98554265933619	16.778660150682143	14.559152922011812
23	8.84450784593438	62.05420827389444	16.93499082942735	12.166293050743835
24	7.574832009773977	62.57381388719202	14.212991244145796	15.638362858888211
25	9.637326813365933	60.28932355338223	16.809290953545233	13.2640586797066
26	12.377250409165303	59.04255319148937	16.550736497545007	12.029459901800326
27	9.817958682757212	59.48046635303743	18.572305174882388	12.12926978932297
28	10.834523566619056	58.37584166496633	19.424607223015713	11.365027545398899
29	11.711711711711711	57.841932841932845	16.052416052416053	14.393939393939394
30	10.866462793068298	60.265035677879716	16.90112130479103	11.967380224260959
31	11.713179873701366	55.693623955999186	19.678142187818292	12.915053982481156
32	12.08656594528379	56.206614944875454	21.947733768885257	9.759085340955492
33	12.380370596619834	55.28405620036653	23.762981062919973	8.572592140093667
34	10.059051109753614	53.57361026267563	26.593361840765628	9.773976786805132
35	10.466300142537161	51.13011606597434	25.778863775198534	12.624720016289961
36	10.242807590287697	48.13303407467864	28.790042848398283	12.834115486635381
37	9.753614335165954	50.45815516188149	28.751781714518426	11.036448788434129
38	10.649562207289758	48.17756057829363	29.953166361229894	11.219710853186724
39	10.669924658928934	49.725106902871104	27.97800855222969	11.62695988597027
40	9.02056607615557	49.82691916106699	27.754021584198735	13.398493178578702
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	489.0
1	384.5
2	280.0
3	280.0
4	259.5
5	239.0
6	245.5
7	252.0
8	252.0
9	273.5
10	295.0
11	319.5
12	344.0
13	344.0
14	313.5
15	283.0
16	255.5
17	228.0
18	228.0
19	226.0
20	224.0
21	215.5
22	207.0
23	207.0
24	209.0
25	211.0
26	200.5
27	190.0
28	190.0
29	177.5
30	165.0
31	184.0
32	203.0
33	203.0
34	202.5
35	202.0
36	194.0
37	186.0
38	186.0
39	181.5
40	177.0
41	168.0
42	159.0
43	159.0
44	164.5
45	170.0
46	151.5
47	133.0
48	133.0
49	108.5
50	84.0
51	81.0
52	78.0
53	78.0
54	73.5
55	69.0
56	46.5
57	24.0
58	24.0
59	18.0
60	12.0
61	9.5
62	7.0
63	7.0
64	3.5
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.12217470983506415
8	0.20362451639177356
9	0.1832620647525962
10	0.12217470983506415
11	0.08144980655670943
12	0.020362451639177357
13	0.061087354917532075
14	0.12217470983506415
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.08144980655670943
24	0.0
25	0.061087354917532075
26	0.4683363877010792
27	0.44797393606190183
28	0.20362451639177356
29	0.5497861942577886
30	0.12217470983506415
31	0.040724903278354714
32	0.2647118713093056
33	0.0
34	0.0
35	0.0
36	0.20362451639177356
37	0.0
38	0.0
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	4911.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.92954591732844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.6350710900474	83.03807778456526
2	2.1800947867298577	3.7466911016086337
3	0.35545023696682465	0.916310323762981
4	0.21327014218009477	0.7330482590103848
5	0.16587677725118483	0.7126858073712076
6	0.07109004739336493	0.3665241295051924
7	0.07109004739336493	0.42761148442272445
8	0.023696682464454978	0.16289961311341886
9	0.047393364928909956	0.3665241295051924
>10	0.18957345971563982	3.868865811443698
>50	0.023696682464454978	1.995520260639381
>100	0.023696682464454978	3.665241295051924
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	180	3.665241295051924	No Hit
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	98	1.995520260639381	No Hit
AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	38	0.7737731622887396	No Hit
AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32	0.6515984524536754	No Hit
AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31	0.631236000814498	No Hit
AATAAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG	28	0.570148645896966	No Hit
AATAAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT	25	0.509061290979434	No Hit
AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13	0.2647118713093056	No Hit
AATAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	12	0.2443494196701283	No Hit
AATAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT	11	0.22398696803095092	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAA	9	0.1832620647525962	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAATATTTTTT	9	0.1832620647525962	No Hit
AATAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	8	0.16289961311341886	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAA	7	0.1425371614742415	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAA	7	0.1425371614742415	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAA	7	0.1425371614742415	No Hit
AATAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6	0.12217470983506415	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT	6	0.12217470983506415	No Hit
AATAAAGGGGGAAAAAAACAAAAAAAAAAAAAAAAAAAAA	6	0.12217470983506415	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTTTTTTT	5	0.10181225819588678	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATATA	5	0.10181225819588678	No Hit
AATAAAGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT	5	0.10181225819588678	No Hit
AATAAAGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAA	5	0.10181225819588678	No Hit
AATAAAGGGGGAACAAAAAAAAAAAAAAAAAAAAAAAAAA	5	0.10181225819588678	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTATTT	5	0.10181225819588678	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAT	5	0.10181225819588678	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTGAA	20	0.00243702	34.24742	22
GGGTAAA	25	1.7807273E-4	33.89796	7
AGGGTAA	25	1.7807273E-4	33.89796	6
AAGGGTA	25	1.7807273E-4	33.89796	5
AAGGGGA	30	1.2308534E-5	33.89796	5
AAGGGAA	50	2.6557245E-10	33.89796	5
AGGGAAA	25	1.7807273E-4	33.89796	6
AGGGGTT	20	0.0025654726	33.897957	6
AGGGGAA	20	0.0025654726	33.897957	6
AAGGGGT	40	5.7911166E-8	33.897957	5
AAGGGAT	20	0.0025654726	33.897957	5
AAAGGGT	45	3.936293E-9	33.897957	4
AAAGGGA	95	0.0	33.897957	4
ATAAAGG	300	0.0	32.76803	2
TAAAGGG	285	0.0	32.708557	3
AATAAAG	335	0.0	31.874203	1
AAAGGGG	130	0.0	31.290424	4
AAGGGGG	40	2.4054552E-6	29.660713	5
AGGGGGG	30	5.192652E-4	28.2483	6
GGTAAAA	30	5.192652E-4	28.2483	8
>>END_MODULE