FastQCFastQC Report
Fri 17 Jun 2016
SRR1525846_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525846_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104780
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT8260.7883183813704906No Hit
ATTCCAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC7370.7033785073487306No Hit
ATTCCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG3400.32448940637526247No Hit
ATTCCAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT3160.3015842718075969No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2880.274861614811987No Hit
ATTCCAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC2380.22714258446268373No Hit
ATTCCAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT2320.22141630082076733No Hit
ATTCCAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC2170.20710059171597633No Hit
ATTCCAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT2080.19851116625310172No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2010.19183050200419927No Hit
ATTCCAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT1890.18037793472036648No Hit
ATTCCAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC1590.15174651651078452No Hit
ATTCCAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG1520.14506585226188204No Hit
ATTCCAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG1370.13075014315709105No Hit
ATTCCAGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG1340.12788700133613284No Hit
ATTCCAGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC1330.1269326207291468No Hit
ATTCCAGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC1270.12120633708723037No Hit
ATTCCAGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG1260.12025195648024432No Hit
ATTCCAGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG1170.11166253101736973No Hit
ATTCCAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA1140.10879938919641152No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1130.10784500858942547No Hit
ATTCCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1130.10784500858942547No Hit
ATTCCAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA1110.10593624737545333No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAA150.00811489750.45309441
GCCCACG150.0081615650.3800138
CGCATTG505.456968E-1250.3800139
GCGCATT505.456968E-1250.3800138
CAGCGTA150.00822408850.2828936
TGACGAC204.05181E-450.2828935
ATATGCC252.0234052E-550.21029734
AATCCGT204.1002565E-450.16201443
ATCCTAG355.1932147E-850.04172514
CCTCGAC150.008381966550.04172515
CACCAAT204.1491623E-450.04172514
TAACAAG150.00839787750.0177448
TATTATA451.3278623E-1050.0177449
ACTAATC402.6448106E-949.9937710
GACGCAC301.0395506E-649.993779
GATTCAT150.0084138149.993779
ATGTGCT150.00842976749.96982633
CCCATTA150.00842976749.96982633
CGCTTCG150.00842976749.96982633
GTGGCGA204.2184078E-449.87428328
Produced by FastQC (version 0.11.5)