##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525846_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	104780
Sequences flagged as poor quality	0
Sequence length	56
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.06075586944073	38.0	35.0	40.0	30.0	40.0
2	35.05324489406375	39.0	35.0	40.0	24.0	40.0
3	35.48503531208246	39.0	36.0	40.0	28.0	40.0
4	34.816930711967935	39.0	35.0	40.0	23.0	40.0
5	35.06610994464592	39.0	35.0	40.0	24.0	40.0
6	33.91577591143348	38.0	34.0	39.0	17.0	40.0
7	34.57750524909334	38.0	35.0	39.0	24.0	40.0
8	34.660727238022524	38.0	35.0	39.0	24.0	40.0
9	34.6935388432907	38.0	35.0	39.0	24.0	40.0
10	34.13354647833556	38.0	34.0	39.0	21.0	40.0
11	33.57413628555068	38.0	33.0	39.0	20.0	40.0
12	32.42995800725329	38.0	30.0	39.0	16.0	40.0
13	31.87737163580836	37.0	29.0	39.0	14.0	40.0
14	32.621378125596486	38.0	30.0	39.0	17.0	40.0
15	31.51923076923077	36.0	28.0	39.0	11.0	40.0
16	31.985235732009926	36.0	29.0	39.0	16.0	40.0
17	30.98496850543997	36.0	27.0	39.0	12.0	40.0
18	30.364611567092958	35.0	25.0	39.0	12.0	40.0
19	31.6896449704142	36.0	28.0	39.0	16.0	40.0
20	32.051756060316855	36.0	29.0	39.0	18.0	40.0
21	32.330368390914295	37.0	30.0	39.0	16.0	40.0
22	31.571988929184958	36.0	28.0	39.0	13.0	40.0
23	32.29843481580454	37.0	30.0	39.0	16.0	40.0
24	31.743567474708914	36.0	29.0	39.0	14.0	40.0
25	32.08974995228097	37.0	30.0	39.0	16.0	40.0
26	32.683117007062414	38.0	31.0	39.0	16.0	40.0
27	32.78349875930521	38.0	32.0	39.0	17.0	40.0
28	32.76914487497614	38.0	32.0	39.0	17.0	40.0
29	32.34815804542852	37.0	31.0	39.0	14.0	40.0
30	32.15001908761214	37.0	31.0	39.0	13.0	40.0
31	32.87891773239168	38.0	33.0	39.0	15.0	40.0
32	32.724890246230196	38.0	32.0	39.0	12.0	40.0
33	32.52089139148693	37.0	32.0	39.0	11.0	40.0
34	32.37626455430426	37.0	32.0	39.0	10.0	40.0
35	32.27057644588662	37.0	32.0	39.0	4.0	40.0
36	31.38290704332888	36.0	30.0	39.0	4.0	40.0
37	31.134663103645735	36.0	30.0	38.0	4.0	40.0
38	30.630731055544953	35.0	29.0	38.0	4.0	40.0
39	29.841534644016033	35.0	27.0	38.0	4.0	40.0
40	27.518763122733347	33.0	18.0	38.0	4.0	39.0
41	25.622914678373736	31.0	13.0	37.0	4.0	39.0
42	26.536209200229052	32.0	18.0	37.0	4.0	39.0
43	26.447079595342622	32.0	18.0	37.0	4.0	39.0
44	25.606413437678945	31.0	13.0	37.0	4.0	39.0
45	26.477075777820193	32.0	18.0	37.0	4.0	39.0
46	24.56370490551632	29.0	11.0	36.0	4.0	39.0
47	23.518285932429855	28.0	7.0	35.0	4.0	39.0
48	24.191515556403893	29.0	8.0	35.0	4.0	39.0
49	23.111299866386716	28.0	4.0	35.0	4.0	39.0
50	23.81784691735064	28.0	4.0	35.0	4.0	39.0
51	24.03493987402176	30.0	4.0	36.0	4.0	39.0
52	23.78076923076923	29.0	4.0	36.0	4.0	39.0
53	23.61081313227715	29.0	4.0	36.0	4.0	39.0
54	23.25091620538271	29.0	4.0	35.0	4.0	39.0
55	23.01369536171025	29.0	4.0	35.0	4.0	39.0
56	22.671578545523953	29.0	4.0	36.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	4.276495027571208
1	2	5.93407052868254
1	3	5.451795281575187
1	4	5.965577166936811
1	5	5.891588491861057
1	6	7.0576184216333
1	7	6.091008483972054
1	8	6.160007537108733
1	9	6.1124976182558655
1	10	6.503755934982163
1	11	2.253592513696912
1	12	-4.398408916498184
1	13	-16.228015627189926
1	14	-4.964010255580909
1	15	2.6688144124513435
1	16	-0.04210883155298717
1	17	-5.02266828647058
1	18	-3.9924404602406014
1	19	0.9514765593726437
1	20	0.14382833635605863
1	21	6.932603665018924
1	22	7.871593845891006
1	23	7.090594506811399
1	24	7.719791870199497
1	25	6.963241954292663
1	26	6.965926531131192
1	27	6.816802765319046
1	28	7.008290046522543
1	29	7.116721832782481
1	30	7.3562454713103484
1	31	6.1303436725640665
1	32	5.989080926829821
1	33	5.842489478981545
1	34	5.050186048641834
1	35	5.825477016364562
1	36	4.753595795072847
1	37	5.036372909745779
1	38	4.834626967126788
1	39	4.699449098011595
1	40	-8.83193196602712
1	41	-0.22265059090606698
1	42	-1.0751764080075397
1	43	-7.82703993298602
1	44	-14.236821498676488
1	45	-1.8384284838739902
1	46	-4.580496758123896
1	47	-9.144565704461096
1	48	-4.134684372572927
1	49	-12.494824363909581
1	50	-3.2773077851250783
1	51	2.956054836218012
1	52	3.044080384792121
1	53	2.990173053755953
1	54	2.912586753986421
1	55	2.7882899466260795
1	56	5.836390963343966
2	1	4.515456066532245
2	2	5.798572693184703
2	3	5.305474935254843
2	4	6.049992751352399
2	5	5.879034379306944
2	6	7.1160599800748585
2	7	6.129103722067292
2	8	5.925375502476697
2	9	5.983493289251534
2	10	6.396829528055758
2	11	5.482596842701238
2	12	1.8526733345840682
2	13	3.185404286229989
2	14	6.118673727103072
2	15	6.929853373490307
2	16	5.388627099182944
2	17	7.638370674568382
2	18	5.3716288038286635
2	19	6.554506862402945
2	20	5.870235262762986
2	21	7.097105829521084
2	22	7.993671767968927
2	23	7.112239528456421
2	24	7.825852476260099
2	25	6.7121597032104106
2	26	5.94990921511388
2	27	6.252300600816881
2	28	5.6593722976047935
2	29	6.303734819795466
2	30	6.3562454713103484
2	31	5.040733282953674
2	32	5.39773893548783
2	33	4.981450517942587
2	34	4.7787574772132615
2	35	5.333269224156769
2	36	6.383033024510077
2	37	6.166675940048812
2	38	6.454540387040204
2	39	6.284730483292979
2	40	5.072829938734785
2	41	8.579946811691336
2	42	7.812269479438349
2	43	6.7145185085724215
2	44	-2.632059593914583
2	45	7.740359394913888
2	46	6.41560713798
2	47	3.0039191440237545
2	48	6.383497445608892
2	49	-0.9281576972429129
2	50	5.762519054701762
2	51	8.392851373014548
2	52	8.275682116393853
2	53	8.068095131678032
2	54	8.238993680393346
2	55	8.456688215024347
2	56	9.20868533563834
3	1	4.911105815556233
3	2	6.468808796549638
3	3	5.889449651744897
3	4	6.713127162953064
3	5	6.310081042255447
3	6	7.892412461948815
3	7	6.593836943855727
3	8	6.468747876523921
3	9	6.282284886202639
3	10	6.933323300132347
3	11	6.72044774435582
3	12	7.521756807348524
3	13	7.8115429737183675
3	14	7.653271235933708
3	15	2.3685594526871796
3	16	7.962711501488204
3	17	9.053540780536036
3	18	9.581258846587538
3	19	7.930561891218709
3	20	7.857556326157667
3	21	8.69684555809517
3	22	9.390436965961122
3	23	8.288666373594928
3	24	9.000048423462186
3	25	8.371575951583715
3	26	7.3821085086628635
3	27	7.518818540954456
3	28	7.182778135121058
3	29	7.828776649131775
3	30	7.602759693284693
3	31	6.327738302474032
3	32	6.644327520726726
3	33	6.274925141478558
3	34	5.785784805712986
3	35	6.68329711038098
3	36	7.5727337123702725
3	37	7.993706986711757
3	38	8.185754367333942
3	39	9.279004511922839
3	40	10.399842394581594
3	41	12.69639968397243
3	42	11.740694837311562
3	43	12.356570934673929
3	44	12.723062175931116
3	45	12.388844243398736
3	46	13.645501170328018
3	47	14.233292633519945
3	48	14.17178523144269
3	49	13.849570505025163
3	50	12.781890685116338
3	51	12.399804338249723
3	52	12.424966369972584
3	53	12.850582582877141
3	54	12.87784848611932
3	55	12.712200392008917
3	56	12.202736274474557
4	1	4.449246804096568
4	2	6.287537816112092
4	3	5.92537201930287
4	4	6.624035061243767
4	5	6.374517512525927
4	6	7.8496220155236855
4	7	6.269069416749971
4	8	6.30090437611878
4	9	6.518770590566568
4	10	6.826675154127795
4	11	7.1952326345823145
4	12	8.168282253966574
4	13	8.331201884857776
4	14	7.831995625330627
4	15	8.618704145359946
4	16	8.814584789291576
4	17	10.015843516192167
4	18	10.236465853571374
4	19	8.849418240333193
4	20	8.497908218737823
4	21	8.155424868972204
4	22	8.647874169341137
4	23	7.981879322624522
4	24	8.713788439667763
4	25	7.545207158899373
4	26	6.970949809739377
4	27	6.638006718598476
4	28	6.35085314380262
4	29	6.698360320081342
4	30	7.013764053357232
4	31	5.69070469518924
4	32	6.093222068706815
4	33	6.613583165169569
4	34	6.372696871152655
4	35	5.648763792481528
4	36	6.721169102268796
4	37	7.2888028697229075
4	38	7.678211055993891
4	39	7.9400763952426665
4	40	2.2546481205529645
4	41	11.705389922040105
4	42	10.504093378809419
4	43	10.34322062406699
4	44	11.64878660391275
4	45	10.89198889748679
4	46	13.097482495327057
4	47	13.269099246939703
4	48	13.367602648582022
4	49	13.558063000298539
4	50	13.482473314278664
4	51	12.684675272385618
4	52	12.28197142456995
4	53	12.41800936838472
4	54	12.442767265299004
4	55	13.022382897700162
4	56	11.963974072059148
5	1	4.764013497125603
5	2	6.258162604271618
5	3	5.819319972453172
5	4	6.456634014281086
5	5	6.296635540620677
5	6	7.154709952856564
5	7	6.268316206189958
5	8	6.1435210296042655
5	9	6.308624844323212
5	10	6.442048181657643
5	11	6.7893651243198185
5	12	7.732549944999597
5	13	7.843724010417979
5	14	7.644785140639634
5	15	8.723212110561615
5	16	8.576796215495772
5	17	9.454701648983185
5	18	9.687977923770596
5	19	8.822189678588757
5	20	8.122131470347814
5	21	8.224305974685308
5	22	8.941739269329844
5	23	7.886146260446985
5	24	8.949242060727531
5	25	8.463747436834069
5	26	7.492133856739716
5	27	7.423304048467038
5	28	7.052694743621853
5	29	7.030789800742667
5	30	7.067731589982092
5	31	5.862399042343988
5	32	6.019332112769632
5	33	5.880633969221844
5	34	6.209024216462041
5	35	5.554499490896024
5	36	7.281708401029768
5	37	7.29957378073108
5	38	7.040241711663683
5	39	8.128642658941683
5	40	10.579997421950171
5	41	12.112989816590627
5	42	10.965962094208809
5	43	11.153712847167956
5	44	12.452462271445608
5	45	10.951718494895744
5	46	12.538524938742114
5	47	13.229141426731069
5	48	13.33265367859746
5	49	13.204957889764891
5	50	12.246605427410891
5	51	11.57169368838381
5	52	11.776680120385866
5	53	11.406146302064531
5	54	11.902865935882367
5	55	12.147415850662163
5	56	10.980958970187423
6	1	4.93352353365632
6	2	6.274959199759891
6	3	6.019711641944383
6	4	6.636613677595967
6	5	6.437410594286931
6	6	7.837043399171485
6	7	6.451459353856883
6	8	6.275747143414378
6	9	6.273487571698645
6	10	6.631706600668675
6	11	6.698377288670368
6	12	7.734319989815631
6	13	8.840635847121924
6	14	7.693630845456418
6	15	8.870276472403969
6	16	8.386911833316734
6	17	9.745403264619842
6	18	9.859107363005336
6	19	8.446902517062753
6	20	8.227467967165499
6	21	7.683726755764653
6	22	8.390012534121013
6	23	7.724017687404391
6	24	8.632027433378457
6	25	7.784200869591203
6	26	6.952081885211076
6	27	6.600270869541866
6	28	6.986073269588783
6	29	7.132322584232291
6	30	6.881688581659116
6	31	5.822780166887348
6	32	5.5271843328577575
6	33	6.1607529764903255
6	34	5.806659135303605
6	35	5.91920404405386
6	36	6.960162812960618
6	37	7.603268278527942
6	38	7.6027393578806866
6	39	8.109887715997385
6	40	4.98420786898064
6	41	12.359477972354568
6	42	10.881451869375457
6	43	11.52561056117391
6	44	12.05130232718319
6	45	11.747334809436474
6	46	13.047168029918247
6	47	13.772243901027757
6	48	13.625464283802145
6	49	13.878817717279667
6	50	13.22461167905854
6	51	12.382788479932785
6	52	12.09958148746303
6	53	11.807946475302959
6	54	12.505660347060015
6	55	12.60099924990142
6	56	12.605483506021418
7	1	4.5700015210777
7	2	6.000136720555183
7	3	5.8599226380896425
7	4	6.549699496220434
7	5	6.274537800616812
7	6	7.346558513799195
7	7	6.024313888245203
7	8	5.911494759563702
7	9	6.193268460317029
7	10	6.43710323284559
7	11	6.830006422368886
7	12	7.822370304281037
7	13	7.922843191410827
7	14	7.748976757406105
7	15	8.404340177009363
7	16	8.51042573065899
7	17	9.072974780140232
7	18	9.957748060915662
7	19	8.228602659079385
7	20	7.951955692870509
7	21	8.386059886217076
7	22	8.92505006708916
7	23	7.852806488378221
7	24	8.780495682516207
7	25	8.172961267237781
7	26	6.63534421022629
7	27	6.653669124766054
7	28	6.20502234648065
7	29	6.483793470821858
7	30	7.007271864272223
7	31	5.4014370952703885
7	32	5.191091819163297
7	33	5.5310106352446375
7	34	5.989901172227924
7	35	4.915673916238884
7	36	6.696620512291112
7	37	6.9088069273410895
7	38	6.718665509229844
7	39	8.222364891895129
7	40	8.147121238832536
7	41	10.78581191433063
7	42	10.261731845029747
7	43	10.890471587751303
7	44	10.725516163661172
7	45	10.834005533721317
7	46	11.99859834032604
7	47	12.358366846858551
7	48	11.70901064208983
7	49	11.903122850166667
7	50	11.270827950107432
7	51	11.164894383767233
7	52	10.402563836823958
7	53	10.588916246047532
7	54	9.998133465339581
7	55	10.464703855298051
7	56	9.338695110809404
8	1	4.776994528070709
8	2	6.006498101110111
8	3	5.606640436420342
8	4	6.238803940163585
8	5	6.017962118234678
8	6	7.095080959095839
8	7	5.90719230015587
8	8	6.004163381264583
8	9	6.002607508365756
8	10	6.424335355561585
8	11	6.814531574635975
8	12	8.01817450008523
8	13	8.399856500682205
8	14	7.856669065098409
8	15	8.969014212651143
8	16	8.544804255360102
8	17	9.389419625617332
8	18	9.552514584714444
8	19	8.05986816776425
8	20	8.03993223245995
8	21	7.302234034649295
8	22	8.15730813160529
8	23	7.373311789528685
8	24	8.67433732474495
8	25	7.455749446800155
8	26	7.320072385277047
8	27	6.726892675408955
8	28	6.501796540029037
8	29	6.86597258203323
8	30	7.230371345436222
8	31	5.37360041582081
8	32	5.762307700056596
8	33	5.870961007453072
8	34	5.608128106583891
8	35	5.931870622758172
8	36	6.747601789078843
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115	18	4.277164934364794
115	19	3.117560475456557
115	20	3.038183980711704
115	21	6.074087181502442
115	22	7.099615823912984
115	23	6.021913188130085
115	24	6.56157508698271
115	25	6.056273922324628
115	26	4.937205252409914
115	27	5.223396171912455
115	28	5.501796540029037
115	29	5.86859495965561
115	30	6.313413303478175
115	31	5.178670345890737
115	32	5.204615392364289
115	33	4.438268699760766
115	34	4.744491742947531
115	35	5.334842650730199
115	36	5.643580810057863
115	37	6.379030252403119
115	38	6.802908685408504
115	39	6.982166380728881
115	40	8.671314787219632
115	41	8.656345413089937
115	42	7.366756658925528
115	43	6.541091935145847
115	44	-1.6898684517234415
115	45	7.215767320321813
115	46	2.4484742708471323
115	47	-6.228481788377179
115	48	2.9553505924620396
115	49	-2.7168523359375527
115	50	3.1933465605292675
115	51	4.480030860194034
115	52	4.935938526650261
115	53	4.631906320489218
115	54	4.749250090649756
115	55	5.401676560012692
115	56	7.885841512794517
116	1	4.560324101722863
116	2	5.859814139910021
116	3	5.582503283250929
116	4	6.085183367188172
116	5	6.006795865132943
116	6	7.480429481541137
116	7	6.003346146309717
116	8	5.930849598273376
116	9	5.83359104096219
116	10	5.9629096844584915
116	11	5.380006422368883
116	12	5.330434820410069
116	13	6.437359320443086
116	14	6.508654176760942
116	15	-2.832756597184183
116	16	5.673328956465443
116	17	6.810071554333778
116	18	6.222264189947921
116	19	6.599570401014873
116	20	6.080987950935029
116	21	6.919930853959016
116	22	8.141179099347227
116	23	6.999580681926609
116	24	8.015979553483955
116	25	6.985864493044232
116	26	5.967602274742422
116	27	5.7407658989596015
116	28	5.638893314222589
116	29	6.343470890176697
116	30	6.184691219110935
116	31	5.262727417851032
116	32	4.937866012711687
116	33	5.07939773201883
116	34	4.697965688356959
116	35	4.709222303335661
116	36	6.406297931645952
116	37	6.626548862824954
116	38	7.094471960842746
116	39	7.743332633830615
116	40	9.268088980768017
116	41	8.790650624008052
116	42	6.847215715997489
116	43	2.6130522329125974
116	44	1.1351935830160151
116	45	6.445295856301961
116	46	1.9534370500034584
116	47	-5.0126008950769325
116	48	0.726752577573702
116	49	-2.0871997304784955
116	50	2.151473111397756
116	51	4.527797609573689
116	52	4.471918675533637
116	53	4.706658181531402
116	54	4.3094237879202275
116	55	4.9227683714270825
116	56	6.443533820486824
117	1	3.014682372141529
117	2	3.450926013623132
117	3	3.222173839187789
117	4	4.142836078238275
117	5	3.7168164553868905
117	6	5.215673132879502
117	7	3.5086652952458905
117	8	3.7362716984792783
117	9	3.6745313292257435
117	10	3.63003391232397
117	11	0.5551951663633936
117	12	-6.230821185080664
117	13	-5.621494488753893
117	14	-1.3659168596151758
117	15	-5.271500591693449
117	16	-7.580102752525633
117	17	-0.2724610469016362
117	18	-3.0004537235730133
117	19	-0.48759500735850025
117	20	-0.73472927624411
117	21	3.590658376265125
117	22	4.1892230252223115
117	23	4.02068569222174
117	24	4.671435970779765
117	25	4.214416311849995
117	26	3.5022625355523083
117	27	3.7119395434345464
117	28	4.182647603858822
117	29	4.480910835269356
117	30	4.307889571890616
117	31	2.7987603622573474
117	32	2.9816203023479204
117	33	3.0100772103990643
117	34	3.7166685023583312
117	35	2.7315284117776635
117	36	5.496895049010398
117	37	5.274283934890846
117	38	5.669111631398685
117	39	5.910971618044751
117	40	8.35748499998559
117	41	6.844356869718414
117	42	4.9434751531972125
117	43	-4.134030814444987
117	44	-2.553207240594144
117	45	3.1335250944625663
117	46	-2.6292671366815092
117	47	-2.3921479094901095
117	48	-0.8832336955903344
117	49	-4.08960192677225
117	50	-1.3588220155754769
117	51	3.1346953446457526
117	52	2.981355875259098
117	53	2.86983594405714
117	54	3.8425397796841274
117	55	3.440956429079794
117	56	5.789278501337886
118	1	3.310542061618243
118	2	4.633789728401737
118	3	4.035859865639772
118	4	4.504538205897852
118	5	4.6135962138687745
118	6	6.248738112752989
118	7	4.347940740904313
118	8	4.6270135041147
118	9	4.429275435033681
118	10	4.496911428137654
118	11	0.40855916866356523
118	12	-7.691143209232475
118	13	-6.939493338667635
118	14	-7.788861080431737
118	15	-8.121989378352449
118	16	-8.628981418425575
118	17	-1.2115064753087665
118	18	-5.915922383722524
118	19	-1.0399239320278504
118	20	-1.745080052552332
118	21	3.758858491273749
118	22	5.184335158632315
118	23	3.692911109128005
118	24	4.68293683334446
118	25	3.67243816348887
118	26	3.6310721962768575
118	27	3.7878452363615196
118	28	3.3666614048939003
118	29	4.161474377535022
118	30	4.549120164185037
118	31	3.4741485163689063
118	32	3.719688157437055
118	33	4.0767822132742815
118	34	4.206030204485991
118	35	3.7924829833705296
118	36	5.625129666606718
118	37	5.547429420802288
118	38	5.603844236344052
118	39	6.886532285094784
118	40	8.272666138570987
118	41	5.994443126187651
118	42	5.361819028987899
118	43	0.5629214569753707
118	44	-1.1690784309334177
118	45	4.9158712704257645
118	46	0.4900543124271728
118	47	-0.1900202499156407
118	48	-0.8214165593051135
118	49	-0.650844019929238
118	50	1.764812256994965
118	51	6.1099684901316635
118	52	6.054961395673132
118	53	4.619692183275081
118	54	6.44259728399695
118	55	5.916229574565705
118	56	8.463804090757094
119	1	3.2405897563718185
119	2	4.801781249334432
119	3	4.289966913864468
119	4	4.886891147074323
119	5	4.709515662729402
119	6	5.97078368773974
119	7	5.018052028662659
119	8	4.964056429392919
119	9	4.877076176952066
119	10	5.103959652832938
119	11	2.1565718872645228
119	12	-6.363904205522886
119	13	-2.6684281558377485
119	14	-5.205925203378211
119	15	-6.83449600262378
119	16	-9.575722276931138
119	17	0.1549482146753327
119	18	-6.4289059555296255
119	19	-1.123389750787787
119	20	-0.9787843450801539
119	21	4.237925793870467
119	22	5.088680680624897
119	23	4.233451649668545
119	24	3.4230004263492297
119	25	4.665813892158717
119	26	5.23547071244008
119	27	5.178147303134175
119	28	4.119443598852566
119	29	5.287335532807944
119	30	5.402591282362042
119	31	4.257667329299487
119	32	3.5066515914593097
119	33	3.494641248780372
119	34	3.588383145662462
119	35	3.521682771393852
119	36	5.098897552139313
119	37	5.1558024997636025
119	38	5.419424522512578
119	39	6.094534404861609
119	40	8.293863806827478
119	41	5.471390661958715
119	42	4.782857714732465
119	43	-3.0637346108571677
119	44	-1.8686014833976472
119	45	5.281001106143833
119	46	-0.5952663023052054
119	47	-2.0429614263862277
119	48	-4.587447295924086
119	49	-3.5286924566012026
119	50	1.1052998033648649
119	51	5.992851373014549
119	52	6.452152704629146
119	53	6.756152172676249
119	54	5.368405445099228
119	55	6.3383281437231
119	56	7.409220094996627
120	1	3.4518197028958824
120	2	4.7311484507604575
120	3	4.313808268588176
120	4	4.993174569534219
120	5	4.794185894458458
120	6	6.4910599800748585
120	7	5.27607341903699
120	8	4.674617926719122
120	9	4.916069046827289
120	10	5.036223467449698
120	11	1.7227483578527547
120	12	-5.325356968446236
120	13	-5.015353289527589
120	14	-4.803295969866625
120	15	0.8003079189448492
120	16	-8.262888052332208
120	17	0.4610979472956558
120	18	-5.451856044656186
120	19	0.031022013918097002
120	20	-0.8691586766309563
120	21	3.559227041642302
120	22	5.384580858878017
120	23	4.358451649668545
120	24	5.469791870199497
120	25	4.378826369877075
120	26	5.5665758817805475
120	27	5.5197248432411286
120	28	5.297251085483584
120	29	5.64918936525001
120	30	6.447154562219435
120	31	4.865733282953677
120	32	5.084860147609042
120	33	5.063268699760766
120	34	4.5469392953950845
120	35	5.021905587793139
120	36	6.240608782085836
120	37	5.8659183642912325
120	38	5.61060099310081
120	39	6.899124422686921
120	40	7.409951150855999
120	41	-4.0927804610359395
120	42	-3.683942641773772
120	43	-6.19229967324576
120	44	-14.313120199975188
120	45	-4.2020648475103535
120	46	-9.12757468020182
120	47	-6.803656613552004
120	48	-5.168775281663837
120	49	-13.551642545727763
120	50	-3.748087005904299
120	51	1.5648210699842444
120	52	1.1203790860908214
120	53	1.8976405862234849
120	54	1.9336906500903162
120	55	2.302900336236469
120	56	5.591261093214097
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	5412.0
5	405.0
6	339.0
7	306.0
8	334.0
9	308.0
10	253.0
11	237.0
12	220.0
13	281.0
14	334.0
15	464.0
16	576.0
17	715.0
18	887.0
19	1045.0
20	1287.0
21	1635.0
22	2033.0
23	2318.0
24	2564.0
25	2898.0
26	3034.0
27	3107.0
28	3333.0
29	3553.0
30	3986.0
31	4612.0
32	5149.0
33	5884.0
34	6480.0
35	7018.0
36	7811.0
37	8588.0
38	9144.0
39	8224.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	0.0	100.0
6	0.0	100.0	0.0	0.0
7	99.64019851116626	0.29394922695170833	0.0448558885283451	0.02099637335369345
8	99.58675319717504	0.31112807787745755	0.07253292613094102	0.02958579881656805
9	98.58274479862568	0.7138766940255774	0.43710631799961824	0.2662721893491124
10	50.26150028631419	30.95533498759305	10.364573391868676	8.41859133422409
11	34.109562893681996	22.296239740408474	21.891582363046382	21.702615002863144
12	30.18705859896927	20.297766749379655	22.690398931093718	26.82477572055736
13	25.086848635235732	15.780683336514603	24.819622065279635	34.31284596297004
14	23.72876503149456	26.363809887383088	26.03836610040084	23.869058980721512
15	30.45830894839347	19.939365447899377	25.00791511162706	24.59441049208009
16	30.091588910006017	26.67061419007325	21.896339308737716	21.341457591183016
17	27.71090088112035	20.828997737534962	29.608698557559208	21.851402823785477
18	24.707959534262265	22.536743653368962	24.164916968887194	28.59037984348158
19	25.768874545263582	24.09983672456102	21.30696737355702	28.82432135661838
20	26.220922453504595	23.581245101974503	28.384655083433685	21.81317736108722
21	25.242412674174457	22.471845772093914	25.143157091047907	27.142584462683715
22	21.85054399694598	21.131895399885476	32.93376598587517	24.08379461729338
23	21.66634853979767	23.11509830120252	30.287268562702806	24.931284596297004
24	22.248840361158305	22.859678927978315	25.366025922461677	29.5254547884017
25	24.117096823457544	24.424442577886378	26.972930665852168	24.48552993280391
26	30.439058859478248	25.661739429357166	21.349451892593866	22.549749818570717
27	21.558796283032024	28.121746936748515	22.881509707857013	27.43794707236245
28	24.360308082880785	27.254072935854246	24.004313923857335	24.381305057407637
29	28.748565965583172	26.126195028680687	22.352772466539196	22.77246653919694
30	28.082473045371636	21.832055236694583	27.4884684805134	22.597003237420378
31	29.891750510700852	25.97414994558888	23.245002768285	20.889096775425266
32	30.482763887695587	25.639850693549466	23.051808574619812	20.825576844135142
33	29.391640068041514	25.108465052273466	24.100265667705127	21.399629211979892
34	23.682650192223093	25.087504542585542	31.26733355010233	19.962511715089036
35	22.59279439457811	26.642004339804803	31.334537772552167	19.430663493064916
36	26.606950160909882	26.24215743384551	26.3815808321476	20.769311573097013
37	27.624082288161517	24.6778449066993	26.64901345977363	21.049059345365556
38	27.988483207866622	26.237050782931426	24.946672661010304	20.827793348191655
39	27.861252891361282	25.573015235801265	21.819502590277377	24.746229282560073
40	22.76482255300302	20.187364002233732	23.862432843580905	33.18538060118234
41	19.601559785336473	17.58522836956102	21.639125902474806	41.1740859426277
42	21.505427811070057	28.057092531255385	23.868009419693287	26.569470237981275
43	19.576704382287062	23.44016234947256	27.907644592498993	29.07548867574139
44	22.287758468561783	24.78996432270687	25.658879042467486	27.26339816626386
45	25.760720879289067	23.668678674944278	26.840700599776163	23.72989984599049
46	21.41311217942579	28.32927927065178	25.065599203233035	25.192009346689392
47	18.569317003877607	23.671632049756212	25.739048642837947	32.02000230352824
48	24.484767452965333	23.81338936109254	26.68990545315634	25.01193773278579
49	21.666092350103376	24.10598054981239	24.30699134696378	29.920935753120453
50	22.29023403502396	26.411431085436654	26.031733886779463	25.26660099275993
51	20.836405772674603	25.55854553115693	28.526282523965023	25.078766172203444
52	21.382871897862575	29.061349399894805	26.863673313250132	22.69210538899249
53	22.53282547946254	27.02120736515714	27.59445699192283	22.85151016345749
54	21.60266408933886	23.68207002803801	28.910728127003573	25.80453775561956
55	19.90943728579908	24.820502019950602	28.962836738210573	26.30722395603975
56	21.07916766323846	24.239177230327673	29.811049988041137	24.87060511839273
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	5.5
4	12.0
5	13.0
6	11.5
7	10.0
8	25.5
9	41.0
10	88.5
11	136.0
12	217.0
13	328.0
14	358.0
15	310.5
16	263.0
17	259.0
18	255.0
19	259.5
20	264.0
21	257.0
22	288.0
23	326.0
24	396.5
25	467.0
26	508.0
27	549.0
28	618.0
29	797.0
30	907.0
31	1054.5
32	1202.0
33	1365.0
34	1528.0
35	1670.5
36	1813.0
37	2005.5
38	2414.0
39	2630.0
40	2902.0
41	3174.0
42	3908.5
43	4643.0
44	5865.5
45	7088.0
46	7427.5
47	7114.0
48	6461.0
49	6362.0
50	6263.0
51	6410.0
52	6557.0
53	6867.5
54	7031.0
55	6884.0
56	6941.5
57	6999.0
58	7032.0
59	7065.0
60	6417.0
61	5769.0
62	4984.5
63	3672.0
64	3144.0
65	2567.5
66	1991.0
67	1820.0
68	1649.0
69	1446.5
70	1244.0
71	1116.0
72	706.5
73	425.0
74	339.5
75	254.0
76	250.0
77	246.0
78	333.5
79	279.0
80	137.0
81	80.0
82	23.0
83	13.0
84	3.0
85	1.5
86	0.0
87	0.0
88	0.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5239549532353502
16	0.06966978430998283
17	0.025768276388623782
18	0.0
19	0.046764649742317234
20	0.13933956861996566
21	0.0
22	0.0
23	0.0
24	0.005726283641916396
25	0.011452567283832793
26	0.0534453139912197
27	0.06776102309601069
28	0.002863141820958198
29	0.1717885092574919
30	0.06394350066806642
31	0.02099637335369345
32	0.027677037602595916
33	0.13170452376407712
34	0.20423744989501816
35	0.15938156136667303
36	0.06012597824012216
37	0.16415346440160336
38	0.22618820385569763
39	0.15079213590379845
40	0.8761213972132087
41	1.1223515938156137
42	0.30349303302156905
43	0.29967551059362474
44	0.4886428707768658
45	0.2300057262836419
46	0.34166825730101164
47	0.564993319335751
48	0.06680664248902461
49	0.29394922695170833
50	0.21282687535789271
51	0.3407138766940256
52	0.2051918305020042
53	0.274861614811987
54	0.2662721893491124
55	0.30731055544951325
56	0.24336705478144685
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	104780.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.80568810841764
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.94574550094367	63.86810460011453
2	6.749249049682342	9.692689444550487
3	1.8235466120843191	3.9282305783546483
4	0.7575958956910072	2.175987783928231
5	0.4040511443685372	1.4506585226188204
6	0.24854461841090938	1.0708150410383661
7	0.1874053005130386	0.9419736590952472
8	0.11962040458279061	0.6871540370299676
9	0.09569632366623249	0.6184386333269708
>10	0.5848108668491985	7.837373544569575
>50	0.053164624259018044	2.6436342813514027
>100	0.027911427735984474	3.5932429853025387
>500	0.0026582312129509026	1.4916968887192212
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTCCAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	826	0.7883183813704906	No Hit
ATTCCAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	737	0.7033785073487306	No Hit
ATTCCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	340	0.32448940637526247	No Hit
ATTCCAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	316	0.3015842718075969	No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	288	0.274861614811987	No Hit
ATTCCAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	238	0.22714258446268373	No Hit
ATTCCAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT	232	0.22141630082076733	No Hit
ATTCCAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC	217	0.20710059171597633	No Hit
ATTCCAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT	208	0.19851116625310172	No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	201	0.19183050200419927	No Hit
ATTCCAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	189	0.18037793472036648	No Hit
ATTCCAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC	159	0.15174651651078452	No Hit
ATTCCAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG	152	0.14506585226188204	No Hit
ATTCCAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG	137	0.13075014315709105	No Hit
ATTCCAGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG	134	0.12788700133613284	No Hit
ATTCCAGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC	133	0.1269326207291468	No Hit
ATTCCAGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC	127	0.12120633708723037	No Hit
ATTCCAGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG	126	0.12025195648024432	No Hit
ATTCCAGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG	117	0.11166253101736973	No Hit
ATTCCAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA	114	0.10879938919641152	No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC	113	0.10784500858942547	No Hit
ATTCCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	113	0.10784500858942547	No Hit
ATTCCAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	111	0.10593624737545333	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.001908761213972132	0.0	0.0	0.0	0.0
11	0.00477190303493033	0.0	0.0	0.0	0.0
12	0.006680664248902462	0.0	0.0	0.0	0.0
13	0.007635044855888528	0.0	0.0	0.0	0.0
14	0.02004199274670739	0.0	0.0	0.0	0.0
15	0.02195075396067952	0.0	0.0	0.0	0.0
16	0.022905134567665585	0.0	0.0	0.0	0.0
17	0.022905134567665585	0.0	0.0	0.0	0.0
18	0.023859515174651652	0.0	0.0	0.0	0.0
19	0.025768276388623782	0.0	0.0	0.0	0.0
20	0.027677037602595916	0.0	0.0	9.54380606986066E-4	0.0
21	0.029585798816568046	0.0	0.0	9.54380606986066E-4	0.0
22	0.03149456003054018	0.0	0.0	9.54380606986066E-4	0.0
23	0.03626646306547051	0.0	0.0	9.54380606986066E-4	0.0
24	0.03912960488642871	0.0	0.0	9.54380606986066E-4	0.0
25	0.0419927467073869	0.0	0.0	9.54380606986066E-4	0.0
26	0.04581026913533117	0.0	0.0	9.54380606986066E-4	0.0
27	0.04867341095628937	0.0	0.0	9.54380606986066E-4	0.0
28	0.051536552777247564	0.0	0.0	9.54380606986066E-4	0.0
29	0.051536552777247564	0.0	0.0	9.54380606986066E-4	0.0
30	0.05535407520519183	0.0	0.0	9.54380606986066E-4	0.0
31	0.062034739454094295	0.0	0.0	9.54380606986066E-4	0.0
32	0.06394350066806642	0.0	0.0	9.54380606986066E-4	0.0
33	0.06394350066806642	0.0	0.0	9.54380606986066E-4	0.0
34	0.06680664248902463	0.0	0.0	9.54380606986066E-4	0.0
35	0.06871540370299675	0.0	0.0	9.54380606986066E-4	0.0
36	0.06871540370299675	0.0	0.0	9.54380606986066E-4	0.0
37	0.07062416491696889	0.0	0.0	9.54380606986066E-4	0.0
38	0.07348730673792708	0.0	0.0	9.54380606986066E-4	9.54380606986066E-4
39	0.07348730673792708	0.0	0.0	9.54380606986066E-4	9.54380606986066E-4
40	0.07348730673792708	0.0	0.0	9.54380606986066E-4	9.54380606986066E-4
41	0.07444168734491315	0.0	0.0	9.54380606986066E-4	9.54380606986066E-4
42	0.08112235159381562	0.0	0.0	9.54380606986066E-4	9.54380606986066E-4
43	0.08875739644970414	0.0	0.0	0.001908761213972132	9.54380606986066E-4
44	0.09543806069860661	0.0	0.0	0.001908761213972132	9.54380606986066E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGGAA	15	0.008114897	50.453094	41
GCCCACG	15	0.00816156	50.38001	38
CGCATTG	50	5.456968E-12	50.38001	39
GCGCATT	50	5.456968E-12	50.38001	38
CAGCGTA	15	0.008224088	50.28289	36
TGACGAC	20	4.05181E-4	50.28289	35
ATATGCC	25	2.0234052E-5	50.210297	34
AATCCGT	20	4.1002565E-4	50.162014	43
ATCCTAG	35	5.1932147E-8	50.041725	14
CCTCGAC	15	0.0083819665	50.041725	15
CACCAAT	20	4.1491623E-4	50.041725	14
TAACAAG	15	0.008397877	50.01774	48
TATTATA	45	1.3278623E-10	50.01774	49
ACTAATC	40	2.6448106E-9	49.99377	10
GACGCAC	30	1.0395506E-6	49.99377	9
GATTCAT	15	0.00841381	49.99377	9
ATGTGCT	15	0.008429767	49.969826	33
CCCATTA	15	0.008429767	49.969826	33
CGCTTCG	15	0.008429767	49.969826	33
GTGGCGA	20	4.2184078E-4	49.874283	28
>>END_MODULE