FastQCFastQC Report
Fri 17 Jun 2016
SRR1525845_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525845_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3920
Sequences flagged as poor quality0
Sequence length40
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC731.862244897959184No Hit
ATTCCAGGGATGAGATGGACTTAAATGAATTGACGGGTAC631.607142857142857No Hit
ATTCCAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC471.1989795918367347No Hit
ATTCCAGGGATGAGATGGCCTTAAATGAATTGACGGGTAC451.1479591836734695No Hit
ATTCCAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC370.9438775510204082No Hit
ATTCCAGGGATGAGATGGCCTCAAATGAATTGACGGGTAC270.6887755102040817No Hit
ATTCCAGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC260.6632653061224489No Hit
ATTCCAGGGATGAGATGGACTCAAATGAATTGACGGGTAC240.6122448979591837No Hit
ATTCCAGGGATGAGATGGACTTACATGAATTGACGGGTAC150.3826530612244898No Hit
ATTCCAGGGATGAGCTGGACTTAAATGAATTGACGGGTAC90.2295918367346939No Hit
ATTCCAGGGCTCGTCTTAATCAGCAACATCTTTTGATACA90.2295918367346939No Hit
ATTCCAGGGATGAGATGGACTTCAATGAATTGACGGGTAC80.20408163265306123No Hit
ATTCCAGGGCTCGTCTTACTCAGCAACATCTTTTGATACA80.20408163265306123No Hit
ATTCCAGGGATGAGATGGCCTCCAATGAATTGACGGGTAC80.20408163265306123No Hit
ATTCCAGGGATGAGATGGACTCACAGGAATTGACGGGTAC70.17857142857142858No Hit
ATTCCAGGGATGAGATGGCCTTACAGGAATTGACGGGTAC60.15306122448979592No Hit
ATTCCAGGGATGAGATGGACTTACAGGAATTGACGGGTAC60.15306122448979592No Hit
ATTCCAGGGATGAGATGGACTTAAATGAATTGACCGGTAC60.15306122448979592No Hit
ATTCCAGGGATGAGATGGACTCAAATGAATTGACCGGTAC50.12755102040816327No Hit
ATTCCAGGGATGAGATGGACTCCAATGAATTGACGGGTAC50.12755102040816327No Hit
ATTCCAGGGATGAGATGGACTCAACTGAATTGACGGGTAC50.12755102040816327No Hit
ATTCCAGGGATGAGATGGACTCCAAGGAATTGACGGGTAC50.12755102040816327No Hit
ATTCCAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT50.12755102040816327No Hit
ATTCCAGGGATAGCCTCTCCACCCAAGCGGCCGGAGAACC40.10204081632653061No Hit
ATTCCAGGGATGAGATGGACTTAAAGGACTTGACGGGTAC40.10204081632653061No Hit
ATTCCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.10204081632653061No Hit
ATTCCAGGGATGAGATGGACTCAAATGACTTGACGGGTAC40.10204081632653061No Hit
ATTCCAGGGCTCGTCTTAATCCGCAACATCTTTTGATACA40.10204081632653061No Hit
ATTCCAGGGATGAGATGGACTTAAAGGAATTGACCGGTAC40.10204081632653061No Hit
ATTCCAGGGGCTCTCTTGCTTCTTGCTCTCTTTTCCTGAA40.10204081632653061No Hit
ATTCCAGGGATGAGATGGACTCAAAGGAATTGACGGGTCC40.10204081632653061No Hit
ATTCCAGGGGAGTGCAGTGCTATTCCAGGGATGAGATGGA40.10204081632653061No Hit
ATTCCAGGGATGAGCTGGACTCAAATGAATTGACGGGTAC40.10204081632653061No Hit
ATTCCAGGGATGAGATGGACTCACATGAATTGACGGGTAC40.10204081632653061No Hit
ATTCCAGGGATGAGATGGCCTCCAAGGAATTGACGGGTAC40.10204081632653061No Hit
ATTCCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT40.10204081632653061No Hit
ATTCCAGGGATGAGATGGCCTTACATGAATTGACGGGTAC40.10204081632653061No Hit
ATTCCAGGGATGAGATGGACTTAAATGACTTGACGGGTAC40.10204081632653061No Hit
ATTCCAGGGATGAGATGGCCTCAAATGAATTGACCGGTAC40.10204081632653061No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGAAT200.002386903234.20779424
ACATGAA200.002386903234.20779423
AGGGAGT251.6192504E-434.2077946
AGGGAAG200.002386903234.2077946
TCAAATG200.002386903234.20779421
TCAAAGG200.002386903234.20779421
CAGGGGC251.6192504E-434.2077945
CAGGGGA251.6192504E-434.2077945
CAGGGAC200.002386903234.2077945
TTGACGG800.034.20779430
GAATTGC200.002386903234.20779427
CTTAAAG200.002386903234.20779420
CAAATGA200.002386903234.20779422
TAAAGGA200.002386903234.20779422
TTAAAGG200.002386903234.20779421
CTCAAAT251.6192504E-434.20779420
CTCAAAG200.002386903234.20779420
CCAGGGG800.034.2077944
AATTGCC200.002386903234.20779428
ATGAATT551.2732926E-1134.2077925
Produced by FastQC (version 0.11.5)