##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525845_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3920
Sequences flagged as poor quality	0
Sequence length	40
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.495408163265306	28.0	22.0	31.0	4.0	33.0
2	22.23188775510204	26.0	17.0	30.0	4.0	33.0
3	15.920408163265305	15.0	13.0	17.0	4.0	29.0
4	17.354336734693877	15.0	11.0	25.0	4.0	30.0
5	14.88954081632653	15.0	12.0	17.0	4.0	24.0
6	21.027295918367347	23.0	15.0	29.0	4.0	32.0
7	20.18469387755102	23.0	11.0	28.0	4.0	31.0
8	21.679846938775512	26.0	15.0	29.0	4.0	32.0
9	19.751020408163264	22.0	11.0	27.0	4.0	31.0
10	16.99591836734694	18.0	10.0	24.0	4.0	28.0
11	24.983673469387757	30.0	16.0	35.0	4.0	38.0
12	22.950255102040817	25.0	14.0	33.0	4.0	37.0
13	24.411734693877552	29.0	16.0	35.0	4.0	37.0
14	24.4515306122449	29.0	15.0	35.0	4.0	38.0
15	22.54515306122449	26.0	11.0	33.0	4.0	36.0
16	21.432908163265306	25.0	6.0	33.0	4.0	36.0
17	21.952551020408162	26.0	4.0	33.0	4.0	36.0
18	21.813520408163264	26.0	4.0	33.0	4.0	36.0
19	18.90765306122449	19.0	4.0	30.0	4.0	35.0
20	19.750510204081632	21.0	4.0	31.0	4.0	35.0
21	19.352551020408164	22.0	4.0	32.0	4.0	35.0
22	18.139795918367348	18.0	4.0	31.0	4.0	35.0
23	18.488520408163264	19.0	4.0	31.0	4.0	35.0
24	18.446938775510205	19.0	4.0	31.0	4.0	35.0
25	17.67066326530612	17.0	4.0	30.0	4.0	35.0
26	16.835969387755103	13.0	4.0	31.0	4.0	36.0
27	16.771173469387755	12.0	4.0	31.0	4.0	35.0
28	15.723469387755102	10.0	4.0	29.0	4.0	34.0
29	15.747704081632653	4.0	4.0	30.0	4.0	35.0
30	15.210714285714285	4.0	4.0	30.0	4.0	35.0
31	13.808163265306122	4.0	4.0	28.0	4.0	33.0
32	12.699489795918367	4.0	4.0	26.0	4.0	32.0
33	11.79438775510204	4.0	4.0	22.0	4.0	31.0
34	11.063775510204081	4.0	4.0	17.0	4.0	31.0
35	10.498979591836735	4.0	4.0	17.0	4.0	30.0
36	10.275510204081632	4.0	4.0	16.0	4.0	31.0
37	9.97704081632653	4.0	4.0	15.0	4.0	31.0
38	10.021428571428572	4.0	4.0	15.0	4.0	31.0
39	9.353826530612245	4.0	4.0	10.0	4.0	30.0
40	9.492857142857142	4.0	4.0	4.0	4.0	31.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	7.803794533406187
1	2	-5.008911584639744
1	3	16.561383600218548
1	4	11.048564003418374
1	5	13.969816052340326
1	6	9.378427828913267
1	7	12.956608386220037
1	8	8.104801832471736
1	9	6.260250563891333
1	10	6.879000126087501
1	11	7.263821992462766
1	12	11.271217725101213
1	13	-1.7482379271214263
1	14	7.444728491573144
1	15	8.79854403956346
1	16	4.025252525252533
1	17	7.144605906499109
1	18	-1.027361689012178
1	19	0.9601969767018339
1	20	11.77628434133289
1	21	6.609514703204031
1	22	9.629396601241258
1	23	3.3086250858095525
1	24	9.323900937250453
1	25	9.704366130094286
1	26	4.391621485310807
1	27	8.444249709298255
1	28	8.137309642892165
1	29	10.123226369100156
1	30	9.825361450846883
1	31	15.439456983146304
1	32	13.59804074027375
1	33	8.989868168508945
1	34	11.337423822132559
1	35	11.034124882668571
1	36	6.8657182084366575
1	37	8.217834026814609
1	38	5.898903038708864
1	39	11.398210257918995
1	40	5.830249092870451
5	1	-3.7676340380223863
5	2	-7.980340156068317
5	3	-1.03861639978145
5	4	-9.3228645680102
5	5	-1.4873268048025317
5	6	-4.4215721710867335
5	7	-11.757677328065679
5	8	-13.038055310385406
5	9	-11.568320864680095
5	10	-4.720999873912499
5	11	-8.021892293251518
5	12	-8.385925132041642
5	13	-6.976809355692854
5	14	-10.898128651283997
5	15	-8.372884531865113
5	16	-4.51760461760461
5	17	-8.198251236358033
5	18	-8.17021883186932
5	19	-1.6969458804410245
5	20	-5.080858515809968
5	21	-3.704771011081684
5	22	0.5436823155269721
5	23	-4.83423205704759
5	24	-5.190384777035263
5	25	-4.524205298477142
5	26	-3.6655213718320496
5	27	0.387106852155398
5	28	-2.034118928536408
5	29	-2.1910593451855593
5	30	1.3967900222754555
5	31	2.2965998402891614
5	32	3.1408978831308936
5	33	-0.32441754577676996
5	34	-1.205433320724584
5	35	-3.908732260188571
5	36	-4.1914246487062
5	37	-0.1535945446139646
5	38	-0.2725255327197065
5	39	-5.030361170652432
5	40	-5.0268937642724065
6	1	1.5656992953109459
6	2	3.086326510598351
6	3	-4.43861639978145
6	4	-2.189531234676865
6	5	-0.5539934714691981
6	6	4.045094495579935
6	7	5.242322671934321
6	8	2.961944689614594
6	9	7.498345801986574
6	10	5.212333459420833
6	11	9.978107706748482
6	12	13.414074867958359
6	13	10.489857310973811
6	14	10.635204682049338
6	15	6.560448801468219
6	16	7.215728715728723
6	17	10.001748763641967
6	18	9.163114501464012
6	19	10.103054119558976
6	20	6.252474817523364
6	21	10.561895655584983
6	22	10.01034898219364
6	23	13.16576794295241
6	24	15.276281889631402
6	25	12.609128034856191
6	26	12.867811961501282
6	27	4.920440185488729
6	28	15.899214404796924
6	29	17.742273988147776
6	30	17.063456688942118
6	31	18.29659984028916
6	32	17.407564549797563
6	33	10.942249120889898
6	34	12.527900012608749
6	35	4.224601073144763
6	36	3.3419086846271338
6	37	3.97973878871937
6	38	3.660807800613627
6	39	4.302972162680902
6	40	4.306439569060927
7	1	1.5656992953109459
7	2	2.419659843931683
7	3	1.7280502668852176
7	4	7.810468765323133
7	5	2.4460065285308
7	6	5.045094495579935
7	7	1.2423226719343212
7	8	-1.704721977052074
7	9	1.4983458019865736
7	10	4.379000126087501
7	11	11.144774373415146
7	12	6.247408201291691
7	13	10.989857310973811
7	14	13.468538015382666
7	15	12.893782134801551
7	16	5.882395382395391
7	17	9.668415430308634
7	18	2.9964478347973476
7	19	10.436387452892308
7	20	13.5858081508567
7	21	7.395228988918316
7	22	5.177015648860305
7	23	14.16576794295241
7	24	9.94294855629807
7	25	2.109128034856191
7	26	2.867811961501282
7	27	3.5871068521553973
7	28	3.8992144047969237
7	29	4.575607321481108
7	30	4.230123355608788
7	31	-0.036733493044172505
7	32	4.907564549797561
7	33	-0.7244175457767703
7	34	-0.4720999873912497
7	35	3.224601073144763
7	36	4.841908684627134
7	37	0.6464054553860361
7	38	-1.0058588660530408
7	39	-1.6970278373190988
7	40	-0.5268937642724065
8	1	5.982365961977614
8	2	9.669659843931683
8	3	0.18638360021854972
8	4	-2.147864568010199
8	5	-0.637326804802532
8	6	9.753427828913267
8	7	1.6173226719343212
8	8	0.21194468961459378
8	9	2.581679135319906
8	10	-1.3709998739124991
8	11	0.22810770674848158
8	12	-1.7109251320416412
8	13	-8.551809355692853
8	14	-5.448128651283998
8	15	-10.897884531865113
8	16	-6.617604617604609
8	17	-13.998251236358033
8	18	-6.67021883186932
8	19	-6.021945880441024
8	20	-7.580858515809968
8	21	-5.604771011081684
8	22	-8.656317684473027
8	23	-9.45923205704759
8	24	-7.140384777035262
8	25	-8.224205298477141
8	26	-5.84052137183205
8	27	-8.787893147844603
8	28	-7.809118928536408
8	29	-8.96605934518556
8	30	-9.728209977724545
8	31	-6.453400159710839
8	32	-5.009102116869107
8	33	-4.84941754577677
8	34	-5.680433320724584
8	35	-4.608732260188571
8	36	-4.8664246487062
8	37	-4.228594544613964
8	38	-4.547525532719707
8	39	-1.6553611706524318
8	40	-1.6518937642724065
9	1	3.375223104834756
9	2	5.562516986788825
9	3	1.1328121716471227
9	4	2.905706860561228
9	5	0.11267319519746799
9	6	3.8069992574846943
9	7	2.528036957648606
9	8	-0.8951981675282639
9	9	1.4031077067484787
9	10	0.8790001260875009
9	11	4.406679135319909
9	12	8.414074867958359
9	13	3.3946192157357196
9	14	6.730442777287429
9	15	7.0842583252777445
9	16	8.739538239538248
9	17	8.430320192213394
9	18	13.25835259670211
9	19	7.388768405273261
9	20	6.2048557699043165
9	21	10.609514703204031
9	22	4.6293966012412575
9	23	10.451482228666695
9	24	9.038186651536165
9	25	8.561508987237143
9	26	10.53447862816795
9	27	0.015678280726824312
9	28	1.4230239286064492
9	29	-0.44820220232841734
9	30	3.9682185937040284
9	31	3.153742697432019
9	32	6.026612168845178
9	33	0.9898681685089432
9	34	3.051709536418274
9	35	-0.10873226018857096
9	36	2.580003922722371
9	37	-0.49645168747110624
9	38	-1.243954104148278
9	39	-1.0303611706524318
9	40	-0.5983223357009777
10	1	1.3232750528867037
10	2	6.510568934840773
10	3	13.834110872945823
10	4	12.204408159262528
10	5	0.658127740652013
10	6	5.651155101640541
10	7	4.969595399207048
10	8	7.5983083259782305
10	9	4.195315498956269
10	10	0.6062728533602275
10	11	10.341744070384848
10	12	12.141347595231085
10	13	10.45955428067078
10	14	9.574598621443275
10	15	5.136206377225797
10	16	9.336940836940844
10	17	11.09265785455106
10	18	12.82978116813068
10	19	15.37578139228625
10	20	15.555505120553665
10	21	9.258865352554679
10	22	14.16186413370879
10	23	13.438495215679684
10	24	12.882342495692011
10	25	9.003067428795585
10	26	10.261751355440676
10	27	9.132561397609944
10	28	8.474971980554502
10	29	8.772577018450804
10	30	5.578608204093639
10	31	6.842054385743708
10	32	6.28635242858544
10	33	6.093764272405046
10	34	6.46729395200269
10	35	5.436722285265974
10	36	4.1903935331119815
10	37	3.646405455386036
10	38	4.05474719455302
10	39	4.515093374802113
10	40	5.518560781182138
11	1	5.482365961977614
11	2	6.169659843931683
11	3	-0.06361639978145028
11	4	-4.897864568010199
11	5	-0.387326804802532
11	6	8.503427828913267
11	7	5.867322671934321
11	8	8.461944689614594
11	9	7.456679135319906
11	10	5.129000126087501
11	11	4.228107706748482
11	12	9.789074867958359
11	13	5.573190644307147
11	14	11.176871348716002
11	15	6.977115468134887
11	16	10.38239538239539
11	17	10.126748763641967
11	18	12.82978116813068
11	19	9.978054119558976
11	20	13.419141484190032
11	21	11.895228988918316
11	22	7.843682315526973
11	23	7.29076794295241
11	24	7.484615222964738
11	25	7.775794701522859
11	26	4.40947862816795
11	27	0.9621068521553973
11	28	5.565881071463592
11	29	-0.8410593451855597
11	30	-1.6032099777245445
11	31	0.2965998402891614
11	32	-3.259102116869107
11	33	-3.8494175457767703
11	34	-0.5554333207245836
11	35	-2.608732260188571
11	36	-0.8664246487062002
11	37	-1.353594544613964
11	38	1.2024744672802932
11	39	-1.4053611706524318
11	40	1.0981062357275935
12	1	0.3990326286442816
12	2	4.252993177265015
12	3	5.228050266885218
12	4	-1.6895312346768652
12	5	3.779339861864136
12	6	5.211761162246599
12	7	-2.257677328065679
12	8	2.1286113562812616
12	9	-1.0016541980134264
12	10	8.212333459420833
12	11	7.978107706748482
12	12	8.247408201291691
12	13	8.656523977640479
12	14	2.96853801538267
12	15	8.893782134801555
12	16	4.882395382395391
12	17	1.5017487636419666
12	18	6.82978116813068
12	19	0.2697207862256441
12	20	9.0858081508567
12	21	5.0618956555849834
12	22	2.177015648860305
12	23	5.66576794295241
12	24	3.109615222964738
12	25	3.275794701522859
12	26	-4.298854705165384
12	27	-6.246226481177937
12	28	-3.1007855952030745
12	29	-6.757726011852226
12	30	-6.769876644391211
12	31	-4.870066826377505
12	32	-8.259102116869107
12	33	-7.72441754577677
12	34	-6.805433320724584
12	35	-6.108732260188571
12	36	-5.9914246487062
12	37	-5.353594544613964
12	38	-5.672525532719707
12	39	-5.030361170652432
12	40	-5.0268937642724065
13	1	-1.9676340380223856
13	2	-2.3803401560683177
13	3	-3.238616399781451
13	4	-0.9228645680101977
13	5	-1.4873268048025317
13	6	1.5784278289132665
13	7	-1.957677328065678
13	8	-1.0380553103854062
13	9	4.431679135319907
13	10	-1.1209998739124991
13	11	0.9781077067484816
13	12	-0.3859251320416419
13	13	2.223190644307145
13	14	1.1018713487160028
13	15	0.4271154681348861
13	16	7.08239538239539
13	17	7.201748763641966
13	18	8.029781168130679
13	19	6.703054119558978
13	20	7.31914148419003
13	21	6.895228988918316
13	22	11.743682315526971
13	23	7.365767942952409
13	24	6.2096152229647394
13	25	10.07579470152286
13	26	-1.6655213718320496
13	27	-0.21289314784460345
13	28	-5.034118928536408
13	29	-0.99105934518556
13	30	0.796790022275454
13	31	-3.7034001597108386
13	32	-3.659102116869107
13	33	-2.1244175457767707
13	34	-1.005433320724583
13	35	-6.108732260188571
13	36	-5.9914246487062
13	37	-5.353594544613964
13	38	-5.672525532719707
13	39	-5.030361170652432
13	40	-5.0268937642724065
15	1	0.34347707308872444
15	2	3.97521539948724
15	3	0.2280502668852158
15	4	-4.7450867902324205
15	5	12.55711763964191
15	6	3.489538940024378
15	7	2.686767116378764
15	8	1.295278022947926
15	9	1.0539013575421272
15	10	4.545666792754169
15	11	3.5336632623040387
15	12	10.858519312402802
15	13	9.156523977640482
15	14	9.301871348716002
15	15	10.004893245912665
15	16	5.993506493506501
15	17	5.779526541419745
15	18	9.274225612575123
15	19	6.9919430084478655
15	20	6.69691926196781
15	21	11.339673433362758
15	22	12.454793426638084
15	23	8.721323498507967
15	24	9.276281889631406
15	25	5.720239145967302
15	26	5.2011452948346175
15	27	2.364884629933176
15	28	1.6769921825747023
15	29	0.07560732148110638
15	30	0.7301233556087876
15	31	2.9632665069558293
15	32	3.185342327575338
15	33	0.7200268986676743
15	34	0.19456667927541638
15	35	1.0023788509225398
15	36	2.4530197957382445
15	37	0.6464054553860361
15	38	1.660807800613627
15	39	1.5251943849031235
15	40	1.5286617912831488
16	1	-4.600967371355718
16	2	-1.580340156068317
16	3	-0.10528306644811636
16	4	4.477135431989801
16	5	0.9460065285308019
16	6	0.21176116224659935
16	7	-3.591010661399011
16	8	-1.0380553103854062
16	9	-0.5016541980134264
16	10	-0.2876665405791652
16	11	4.978107706748482
16	12	-0.41925846537497335
16	13	2.6565239776404788
16	14	2.6352046820493342
16	15	5.060448801468219
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104	27	-5.662893147844603
104	28	-5.934118928536408
104	29	-7.84105934518556
104	30	-4.8532099777245445
104	31	-2.4534001597108386
104	32	-2.509102116869107
104	33	-1.9744175457767703
104	34	-1.3054333207245836
104	35	0.14126773981142904
104	36	-2.7414246487062
104	37	-2.103594544613964
104	38	-2.422525532719707
104	39	-1.7803611706524318
104	40	-1.7768937642724065
105	1	-8.767634038022386
105	2	-14.580340156068317
105	3	-7.68861639978145
105	4	-7.522864568010199
105	5	-6.637326804802532
105	6	-8.621572171086733
105	7	-9.257677328065679
105	8	-8.038055310385406
105	9	-4.168320864680094
105	10	-3.120999873912499
105	11	-5.521892293251518
105	12	-9.835925132041641
105	13	-11.426809355692853
105	14	-6.448128651283998
105	15	-8.522884531865113
105	16	-1.8676046176046093
105	17	-4.998251236358033
105	18	-0.9202188318693203
105	19	-3.1469458804410237
105	20	-8.080858515809968
105	21	-2.3547710110816844
105	22	0.3436823155269728
105	23	-3.58423205704759
105	24	-7.390384777035262
105	25	-6.724205298477141
105	26	-4.46552137183205
105	27	-5.912893147844603
105	28	-4.684118928536408
105	29	-9.84105934518556
105	30	-3.6032099777245445
105	31	-9.703400159710839
105	32	-8.259102116869107
105	33	-7.72441754577677
105	34	-6.805433320724584
105	35	-6.108732260188571
105	36	-5.9914246487062
105	37	-5.353594544613964
105	38	-5.672525532719707
105	39	-5.030361170652432
105	40	-5.0268937642724065
106	1	6.232365961977614
106	2	2.752993177265015
106	3	0.7280502668852176
106	4	-3.022864568010199
106	5	1.2793398618641358
106	6	7.045094495579935
106	7	-3.924343994732345
106	8	1.6286113562812616
106	9	1.4983458019865736
106	10	4.545666792754169
106	11	8.311441040081817
106	12	-3.752591798708309
106	13	5.823190644307147
106	14	4.301871348716002
106	15	3.727115468134887
106	16	-3.9509379509379414
106	17	1.1684154303086345
106	18	2.3297811681306797
106	19	-5.230279213774358
106	20	-7.580858515809968
106	21	-8.77143767774835
106	22	-4.656317684473027
106	23	-7.500898723714256
106	24	-5.557051443701928
106	25	-3.724205298477141
106	26	-4.632188038498716
106	27	-4.912893147844603
106	28	-4.6007855952030745
106	29	-4.424392678518894
106	30	-4.6032099777245445
106	31	-6.703400159710839
106	32	-4.59243545020244
106	33	-3.8910842124434373
106	34	-2.3054333207245836
106	35	-2.942065593521904
106	36	-0.3247579820395341
106	37	-0.3535945446139639
106	38	-0.005858866053040757
106	39	-2.863694503985765
106	40	0.13977290239425955
107	1	-7.017634038022386
107	2	-8.830340156068317
107	3	-7.68861639978145
107	4	-9.772864568010199
107	5	-7.137326804802532
107	6	-8.371572171086733
107	7	-6.257677328065679
107	8	-8.288055310385406
107	9	-3.168320864680094
107	10	-7.120999873912499
107	11	-10.521892293251518
107	12	-6.085925132041641
107	13	-10.426809355692853
107	14	-4.198128651283998
107	15	-2.772884531865113
107	16	-16.11760461760461
107	17	-16.998251236358033
107	18	-16.17021883186932
107	19	-13.896945880441024
107	20	-14.080858515809968
107	21	-15.104771011081684
107	22	-12.656317684473027
107	23	-13.83423205704759
107	24	-13.390384777035262
107	25	-12.724205298477141
107	26	-12.46552137183205
107	27	-12.412893147844603
107	28	-11.434118928536408
107	29	-11.59105934518556
107	30	-11.603209977724545
107	31	-9.703400159710839
107	32	-8.259102116869107
107	33	-7.72441754577677
107	34	-6.805433320724584
107	35	-6.108732260188571
107	36	-5.9914246487062
107	37	-5.353594544613964
107	38	-5.672525532719707
107	39	-5.030361170652432
107	40	-5.0268937642724065
108	1	0.23236596197761372
108	2	0.419659843931683
108	3	-1.4386163997814503
108	4	-2.522864568010199
108	5	-1.887326804802532
108	6	-5.621572171086733
108	7	3.242322671934321
108	8	-3.538055310385406
108	9	1.8316791353199058
108	10	0.8790001260875009
108	11	7.978107706748482
108	12	2.414074867958359
108	13	4.323190644307147
108	14	3.801871348716002
108	15	-1.7728845318651132
108	16	5.882395382395391
108	17	12.001748763641967
108	18	8.32978116813068
108	19	-3.3969458804410237
108	20	-2.580858515809968
108	21	11.895228988918316
108	22	14.343682315526973
108	23	2.66576794295241
108	24	11.109615222964738
108	25	10.275794701522859
108	26	19.03447862816795
108	27	14.587106852155397
108	28	15.065881071463592
108	29	17.40894065481444
108	30	17.896790022275454
108	31	3.2965998402891614
108	32	-8.259102116869107
108	33	-7.72441754577677
108	34	-6.805433320724584
108	35	-6.108732260188571
108	36	-5.9914246487062
108	37	-5.353594544613964
108	38	-5.672525532719707
108	39	-5.030361170652432
108	40	-5.0268937642724065
109	1	-0.43430070468905413
109	2	-6.580340156068317
109	3	-2.6052830664481164
109	4	-7.856197901343533
109	5	-2.720660138135866
109	6	4.378427828913267
109	7	-5.257677328065679
109	8	3.795278022947926
109	9	-3.0016541980134264
109	10	0.3790001260875009
109	11	-1.6885589599181863
109	12	-2.085925132041641
109	13	-3.6768093556928534
109	14	0.13520468204933422
109	15	1.560448801468219
109	16	2.0490620490620586
109	17	2.1684154303086345
109	18	-1.3368854985359881
109	19	2.769720786225644
109	20	-3.2475251824766342
109	21	5.395228988918316
109	22	5.677015648860305
109	23	5.832434609619078
109	24	5.109615222964738
109	25	0.2757947015228588
109	26	4.2011452948346175
109	27	4.920440185488729
109	28	3.8992144047969237
109	29	2.2422739881477725
109	30	7.563456688942123
109	31	6.629933173622494
109	32	9.574231216464225
109	33	5.77558245422323
109	34	8.027900012608749
109	35	5.557934406478095
109	36	8.841908684627132
109	37	6.646405455386036
109	38	7.827474467280293
109	39	4.802972162680902
109	40	9.306439569060926
110	1	5.232365961977614
110	2	3.419659843931683
110	3	-2.4386163997814503
110	4	-6.522864568010199
110	5	-1.887326804802532
110	6	0.3784278289132672
110	7	9.242322671934321
110	8	4.961944689614594
110	9	7.831679135319906
110	10	7.879000126087501
110	11	13.978107706748482
110	12	16.41407486795836
110	13	14.823190644307147
110	14	15.301871348716002
110	15	12.227115468134887
110	16	6.882395382395391
110	17	10.001748763641967
110	18	2.8297811681306797
110	19	-9.896945880441024
110	20	12.919141484190032
110	21	11.895228988918316
110	22	14.343682315526973
110	23	-13.83423205704759
110	24	-13.390384777035262
110	25	-12.724205298477141
110	26	-12.46552137183205
110	27	-12.412893147844603
110	28	-11.434118928536408
110	29	-11.59105934518556
110	30	-11.603209977724545
110	31	-9.703400159710839
110	32	-8.259102116869107
110	33	-7.72441754577677
110	34	-6.805433320724584
110	35	-6.108732260188571
110	36	-5.9914246487062
110	37	-5.353594544613964
110	38	-5.672525532719707
110	39	-5.030361170652432
110	40	-5.0268937642724065
111	1	-0.7676340380223863
111	2	-0.24700682273498487
111	3	-1.7719497331147842
111	4	-5.856197901343533
111	5	-0.5539934714691981
111	6	4.045094495579935
111	7	-6.091010661399013
111	8	1.6286113562812616
111	9	-8.501654198013428
111	10	2.879000126087501
111	11	1.6447743734151494
111	12	7.080741534625027
111	13	6.4898573109738145
111	14	6.96853801538267
111	15	1.560448801468219
111	16	14.215728715728726
111	17	13.335082096975302
111	18	7.163114501464012
111	19	15.103054119558976
111	20	15.252474817523368
111	21	13.228562322251651
111	22	16.010348982193637
111	23	11.16576794295241
111	24	14.942948556298074
111	25	7.275794701522859
111	26	1.8678119615012818
111	27	7.587106852155397
111	28	7.232547738130259
111	29	8.075607321481108
111	30	6.730123355608788
111	31	10.96326650695583
111	32	13.407564549797561
111	33	13.942249120889898
111	34	11.194566679275416
111	35	14.557934406478097
111	36	15.675242017960468
111	37	9.313072122052704
111	38	7.994141133946961
111	39	9.636305496014236
111	40	9.973106235727593
112	1	-3.7676340380223863
112	2	-11.080340156068317
112	3	-2.4386163997814503
112	4	-1.522864568010199
112	5	0.11267319519746799
112	6	-1.6215721710867328
112	7	-0.2576773280656788
112	8	-0.5380553103854062
112	9	-1.1683208646800942
112	10	5.879000126087501
112	11	-1.0218922932515184
112	12	-0.0859251320416412
112	13	10.823190644307147
112	14	-2.198128651283998
112	15	10.727115468134887
112	16	3.8823953823953907
112	17	5.501748763641967
112	18	9.32978116813068
112	19	9.603054119558976
112	20	-1.0808585158099682
112	21	-0.6047710110816844
112	22	-0.15631768447302719
112	23	2.66576794295241
112	24	8.109615222964738
112	25	-1.2242052984771412
112	26	-8.96552137183205
112	27	-7.912893147844603
112	28	-6.934118928536408
112	29	-5.59105934518556
112	30	-2.6032099777245445
112	31	-2.7034001597108386
112	32	-6.759102116869107
112	33	-0.7244175457767703
112	34	3.1945666792754164
112	35	-1.108732260188571
112	36	9.5085753512938
112	37	7.146405455386036
112	38	9.827474467280293
112	39	9.969638829347568
112	40	10.473106235727593
120	1	1.2323659619776137
120	2	2.419659843931683
120	3	20.561383600218548
120	4	17.4771354319898
120	5	19.112673195197466
120	6	13.378427828913267
120	7	6.242322671934321
120	8	-2.038055310385406
120	9	10.831679135319906
120	10	-12.1209998739125
120	11	-20.02189229325152
120	12	-17.58592513204164
120	13	-19.176809355692853
120	14	-18.698128651283998
120	15	-16.772884531865113
120	16	-16.11760461760461
120	17	-16.998251236358033
120	18	-16.17021883186932
120	19	-13.896945880441024
120	20	-14.080858515809968
120	21	-15.104771011081684
120	22	-12.656317684473027
120	23	-13.83423205704759
120	24	-13.390384777035262
120	25	-12.724205298477141
120	26	-12.46552137183205
120	27	-12.412893147844603
120	28	-11.434118928536408
120	29	-11.59105934518556
120	30	-11.603209977724545
120	31	-9.703400159710839
120	32	-8.259102116869107
120	33	-7.72441754577677
120	34	-6.805433320724584
120	35	-6.108732260188571
120	36	-5.9914246487062
120	37	-5.353594544613964
120	38	-5.672525532719707
120	39	-5.030361170652432
120	40	-5.0268937642724065
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	533.0
5	48.0
6	33.0
7	48.0
8	29.0
9	43.0
10	95.0
11	127.0
12	130.0
13	108.0
14	116.0
15	146.0
16	138.0
17	192.0
18	199.0
19	195.0
20	203.0
21	234.0
22	218.0
23	238.0
24	220.0
25	160.0
26	143.0
27	110.0
28	101.0
29	61.0
30	36.0
31	12.0
32	3.0
33	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	0.0	100.0
6	0.0	100.0	0.0	0.0
7	99.18263090676885	0.6130268199233717	0.1277139208173691	0.07662835249042146
8	97.90656114373245	0.7403625223385244	0.5871840694408986	0.7658922644881286
9	96.55348480980342	2.042379371968343	0.7403625223385244	0.6637732958897115
10	21.224489795918366	54.71938775510205	9.974489795918368	14.081632653061224
11	15.714285714285714	23.341836734693878	45.255102040816325	15.688775510204081
12	52.755102040816325	13.928571428571429	16.377551020408163	16.93877551020408
13	19.79591836734694	45.816326530612244	15.76530612244898	18.622448979591837
14	42.525510204081634	20.229591836734695	20.918367346938776	16.3265306122449
15	17.066326530612244	44.69387755102041	20.739795918367346	17.5
16	16.760204081632654	18.239795918367346	45.28061224489796	19.71938775510204
17	45.40816326530612	17.959183673469386	15.535714285714286	21.096938775510203
18	42.83163265306123	24.158163265306122	15.051020408163266	17.959183673469386
19	16.19754350051177	30.885363357215965	12.18014329580348	40.73694984646878
20	18.647959183673468	20.612244897959183	15.76530612244898	44.974489795918366
21	17.5	17.47448979591837	43.11224489795919	21.913265306122447
22	15.178571428571427	19.132653061224488	30.22959183673469	35.45918367346938
23	18.6734693877551	42.704081632653065	18.724489795918366	19.897959183673468
24	14.209183673469388	42.704081632653065	18.801020408163264	24.285714285714285
25	16.60705160960654	45.09453244762391	17.78231987736331	20.516096065406234
26	25.250707122653637	20.930830547698637	34.173309334019024	19.645152995628695
27	37.917836182699666	18.423067108956367	22.582291400867568	21.0768053074764
28	11.513913709471534	43.757978044421755	26.01480725044677	18.713300995659942
29	11.483375959079284	39.71867007672634	28.797953964194374	20.0
30	10.048580925594477	15.87829199693173	54.94758373817438	19.125543339299412
31	9.8285129255183	17.63501407729716	57.00025595085744	15.536217046327106
32	34.42204644041848	14.927277366675174	33.809645317683085	16.84103087522327
33	9.642857142857144	38.36734693877551	35.22959183673469	16.760204081632654
34	7.576530612244898	16.377551020408163	37.066326530612244	38.9795918367347
35	30.620056136769584	17.019647869354426	36.23373309517734	16.126562898698648
36	30.714285714285715	18.316326530612244	36.607142857142854	14.362244897959183
37	29.242153610614952	16.407246746619037	40.852258229140084	13.498341413625925
38	6.73469387755102	18.418367346938773	59.132653061224495	15.714285714285714
39	8.239795918367347	35.433673469387756	36.0969387755102	20.229591836734695
40	6.326530612244897	21.479591836734695	36.58163265306123	35.61224489795919
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.0
6	0.5
7	0.0
8	0.0
9	1.0
10	2.0
11	3.0
12	4.0
13	4.0
14	3.5
15	3.0
16	11.5
17	20.0
18	20.0
19	30.5
20	41.0
21	49.0
22	57.0
23	57.0
24	57.0
25	57.0
26	75.5
27	94.0
28	94.0
29	99.5
30	105.0
31	115.5
32	126.0
33	126.0
34	162.0
35	198.0
36	206.5
37	215.0
38	215.0
39	236.5
40	258.0
41	302.0
42	346.0
43	346.0
44	422.5
45	499.0
46	481.0
47	463.0
48	463.0
49	405.5
50	348.0
51	301.5
52	255.0
53	255.0
54	244.5
55	234.0
56	217.0
57	200.0
58	200.0
59	172.5
60	145.0
61	121.0
62	97.0
63	97.0
64	79.5
65	62.0
66	46.5
67	31.0
68	31.0
69	28.0
70	25.0
71	21.5
72	18.0
73	18.0
74	15.0
75	12.0
76	7.5
77	3.0
78	3.0
79	1.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.12755102040816327
8	0.07653061224489796
9	0.07653061224489796
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.30612244897959184
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.15306122448979592
26	0.7908163265306122
27	0.025510204081632654
28	0.07653061224489796
29	0.25510204081632654
30	0.2295918367346939
31	0.33163265306122447
32	0.025510204081632654
33	0.0
34	0.0
35	0.025510204081632654
36	0.0
37	0.025510204081632654
38	0.0
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	3920.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.90306122448979
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.80085132259045	79.54081632653062
2	3.253268470659775	5.459183673469388
3	0.7601094557616297	1.913265306122449
4	0.486470051687443	1.6326530612244898
5	0.15202189115232592	0.6377551020408163
6	0.09121313469139557	0.4591836734693878
7	0.030404378230465188	0.17857142857142858
8	0.09121313469139557	0.6122448979591837
9	0.060808756460930376	0.4591836734693878
>10	0.2128306476132563	5.637755102040817
>50	0.060808756460930376	3.4693877551020407
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATTCCAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC	73	1.862244897959184	No Hit
ATTCCAGGGATGAGATGGACTTAAATGAATTGACGGGTAC	63	1.607142857142857	No Hit
ATTCCAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC	47	1.1989795918367347	No Hit
ATTCCAGGGATGAGATGGCCTTAAATGAATTGACGGGTAC	45	1.1479591836734695	No Hit
ATTCCAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC	37	0.9438775510204082	No Hit
ATTCCAGGGATGAGATGGCCTCAAATGAATTGACGGGTAC	27	0.6887755102040817	No Hit
ATTCCAGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC	26	0.6632653061224489	No Hit
ATTCCAGGGATGAGATGGACTCAAATGAATTGACGGGTAC	24	0.6122448979591837	No Hit
ATTCCAGGGATGAGATGGACTTACATGAATTGACGGGTAC	15	0.3826530612244898	No Hit
ATTCCAGGGATGAGCTGGACTTAAATGAATTGACGGGTAC	9	0.2295918367346939	No Hit
ATTCCAGGGCTCGTCTTAATCAGCAACATCTTTTGATACA	9	0.2295918367346939	No Hit
ATTCCAGGGATGAGATGGACTTCAATGAATTGACGGGTAC	8	0.20408163265306123	No Hit
ATTCCAGGGCTCGTCTTACTCAGCAACATCTTTTGATACA	8	0.20408163265306123	No Hit
ATTCCAGGGATGAGATGGCCTCCAATGAATTGACGGGTAC	8	0.20408163265306123	No Hit
ATTCCAGGGATGAGATGGACTCACAGGAATTGACGGGTAC	7	0.17857142857142858	No Hit
ATTCCAGGGATGAGATGGCCTTACAGGAATTGACGGGTAC	6	0.15306122448979592	No Hit
ATTCCAGGGATGAGATGGACTTACAGGAATTGACGGGTAC	6	0.15306122448979592	No Hit
ATTCCAGGGATGAGATGGACTTAAATGAATTGACCGGTAC	6	0.15306122448979592	No Hit
ATTCCAGGGATGAGATGGACTCAAATGAATTGACCGGTAC	5	0.12755102040816327	No Hit
ATTCCAGGGATGAGATGGACTCCAATGAATTGACGGGTAC	5	0.12755102040816327	No Hit
ATTCCAGGGATGAGATGGACTCAACTGAATTGACGGGTAC	5	0.12755102040816327	No Hit
ATTCCAGGGATGAGATGGACTCCAAGGAATTGACGGGTAC	5	0.12755102040816327	No Hit
ATTCCAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.12755102040816327	No Hit
ATTCCAGGGATAGCCTCTCCACCCAAGCGGCCGGAGAACC	4	0.10204081632653061	No Hit
ATTCCAGGGATGAGATGGACTTAAAGGACTTGACGGGTAC	4	0.10204081632653061	No Hit
ATTCCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4	0.10204081632653061	No Hit
ATTCCAGGGATGAGATGGACTCAAATGACTTGACGGGTAC	4	0.10204081632653061	No Hit
ATTCCAGGGCTCGTCTTAATCCGCAACATCTTTTGATACA	4	0.10204081632653061	No Hit
ATTCCAGGGATGAGATGGACTTAAAGGAATTGACCGGTAC	4	0.10204081632653061	No Hit
ATTCCAGGGGCTCTCTTGCTTCTTGCTCTCTTTTCCTGAA	4	0.10204081632653061	No Hit
ATTCCAGGGATGAGATGGACTCAAAGGAATTGACGGGTCC	4	0.10204081632653061	No Hit
ATTCCAGGGGAGTGCAGTGCTATTCCAGGGATGAGATGGA	4	0.10204081632653061	No Hit
ATTCCAGGGATGAGCTGGACTCAAATGAATTGACGGGTAC	4	0.10204081632653061	No Hit
ATTCCAGGGATGAGATGGACTCACATGAATTGACGGGTAC	4	0.10204081632653061	No Hit
ATTCCAGGGATGAGATGGCCTCCAAGGAATTGACGGGTAC	4	0.10204081632653061	No Hit
ATTCCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	4	0.10204081632653061	No Hit
ATTCCAGGGATGAGATGGCCTTACATGAATTGACGGGTAC	4	0.10204081632653061	No Hit
ATTCCAGGGATGAGATGGACTTAAATGACTTGACGGGTAC	4	0.10204081632653061	No Hit
ATTCCAGGGATGAGATGGCCTCAAATGAATTGACCGGTAC	4	0.10204081632653061	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGAAT	20	0.0023869032	34.207794	24
ACATGAA	20	0.0023869032	34.207794	23
AGGGAGT	25	1.6192504E-4	34.207794	6
AGGGAAG	20	0.0023869032	34.207794	6
TCAAATG	20	0.0023869032	34.207794	21
TCAAAGG	20	0.0023869032	34.207794	21
CAGGGGC	25	1.6192504E-4	34.207794	5
CAGGGGA	25	1.6192504E-4	34.207794	5
CAGGGAC	20	0.0023869032	34.207794	5
TTGACGG	80	0.0	34.207794	30
GAATTGC	20	0.0023869032	34.207794	27
CTTAAAG	20	0.0023869032	34.207794	20
CAAATGA	20	0.0023869032	34.207794	22
TAAAGGA	20	0.0023869032	34.207794	22
TTAAAGG	20	0.0023869032	34.207794	21
CTCAAAT	25	1.6192504E-4	34.207794	20
CTCAAAG	20	0.0023869032	34.207794	20
CCAGGGG	80	0.0	34.207794	4
AATTGCC	20	0.0023869032	34.207794	28
ATGAATT	55	1.2732926E-11	34.20779	25
>>END_MODULE