FastQCFastQC Report
Fri 17 Jun 2016
SRR1525844_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525844_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086
Sequences flagged as poor quality0
Sequence length40
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT90.4314477468839885No Hit
ATTCCAGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT50.23969319271332695No Hit
ATTCCAGGGGGGCTGGTGAGATGGCCCAGTGGGTAAGAGC40.19175455417066153No Hit
ATTCCAGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.19175455417066153No Hit
ATTCCAGGGGCGTCCCCTTTTTTTTTTTTTTTTTTTTTTT40.19175455417066153No Hit
ATTCCAGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTTT30.14381591562799617No Hit
ATTCCAGGGCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.14381591562799617No Hit
ATTCCAGGGGTGTCAAAGCCTAGGGGTCCCTGGGTTGGCA30.14381591562799617No Hit
ATTCCAGGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTT30.14381591562799617No Hit
ATTCCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT30.14381591562799617No Hit
ATTCCAGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTTT30.14381591562799617No Hit
ATTCCAGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTTT30.14381591562799617No Hit
ATTCCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGCGCC30.14381591562799617No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG200.002191459834.06
AGGGGCC200.002191459834.06
CAGGGGG308.867741E-634.05
CAGGGGC355.460952E-734.05
CAGGGAT200.002191459834.05
CCAGGGA403.2843673E-834.04
TCCAGGG1850.032.1621633
TTCCAGG2150.031.6279072
ATTCCAG2150.031.6279071
CCAGGGG1200.029.754
CAGGGGT406.3651554E-525.55
Produced by FastQC (version 0.11.5)