##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525844_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2086 Sequences flagged as poor quality 0 Sequence length 40 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.546500479386385 4.0 4.0 24.0 4.0 30.0 2 12.200862895493769 4.0 4.0 22.0 4.0 28.0 3 11.132310642377757 4.0 4.0 19.0 4.0 28.0 4 8.174496644295303 4.0 4.0 13.0 4.0 15.0 5 8.805848513902205 4.0 4.0 15.0 4.0 15.0 6 8.74496644295302 4.0 4.0 11.0 4.0 22.0 7 10.300095877277085 4.0 4.0 21.0 4.0 26.0 8 10.912751677852349 4.0 4.0 22.0 4.0 29.0 9 9.791466922339406 4.0 4.0 19.0 4.0 27.0 10 8.015340364333653 4.0 4.0 11.0 4.0 20.0 11 9.98705656759348 4.0 4.0 15.0 4.0 29.0 12 9.870086289549377 4.0 4.0 14.0 4.0 28.0 13 9.97698945349952 4.0 4.0 15.0 4.0 29.0 14 9.671620325982742 4.0 4.0 11.0 4.0 28.0 15 9.267497603068072 4.0 4.0 11.0 4.0 28.0 16 8.37967401725791 4.0 4.0 4.0 4.0 24.0 17 7.943432406519655 4.0 4.0 4.0 4.0 22.0 18 7.720517737296261 4.0 4.0 4.0 4.0 21.0 19 7.716203259827421 4.0 4.0 4.0 4.0 21.0 20 7.929530201342282 4.0 4.0 4.0 4.0 22.0 21 8.070469798657719 4.0 4.0 4.0 4.0 23.0 22 7.669223394055609 4.0 4.0 4.0 4.0 22.0 23 7.886385426653883 4.0 4.0 4.0 4.0 22.0 24 7.654362416107382 4.0 4.0 4.0 4.0 22.0 25 7.775167785234899 4.0 4.0 4.0 4.0 22.0 26 8.283317353787153 4.0 4.0 4.0 4.0 27.0 27 8.38255033557047 4.0 4.0 4.0 4.0 28.0 28 8.219558964525408 4.0 4.0 4.0 4.0 27.0 29 8.206136145733462 4.0 4.0 4.0 4.0 27.0 30 8.323106423777565 4.0 4.0 4.0 4.0 27.0 31 8.467881112176414 4.0 4.0 4.0 4.0 31.0 32 8.356184084372003 4.0 4.0 4.0 4.0 31.0 33 8.418024928092041 4.0 4.0 4.0 4.0 31.0 34 8.364813039309684 4.0 4.0 4.0 4.0 32.0 35 8.36960690316395 4.0 4.0 4.0 4.0 31.0 36 7.958772770853308 4.0 4.0 4.0 4.0 31.0 37 7.877756471716204 4.0 4.0 4.0 4.0 31.0 38 7.817353787152445 4.0 4.0 4.0 4.0 30.0 39 7.719079578139981 4.0 4.0 4.0 4.0 30.0 40 7.751677852348993 4.0 4.0 4.0 4.0 30.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -8.02103825136612 1 2 -6.801502732240436 1 3 -6.323907103825135 1 4 -3.8707650273224035 1 5 -4.115710382513662 1 6 -3.801092896174863 1 7 -5.634699453551914 1 8 -5.446857923497268 1 9 -5.113524590163934 1 10 -3.398224043715846 1 11 -4.831284153005464 1 12 -4.824726775956282 1 13 -4.486065573770489 1 14 -5.035109289617488 1 15 -4.330327868852459 1 16 -3.621174863387977 1 17 -2.9836065573770503 1 18 -3.020628415300547 1 19 -2.806010928961748 1 20 -3.2127049180327862 1 21 -3.1140710382513657 1 22 -2.6334699453551913 1 23 -3.194262295081968 1 24 -3.090573770491803 1 25 -3.2979508196721303 1 26 -3.8588797814207645 1 27 -4.086885245901639 1 28 -4.041939890710381 1 29 -4.050136612021857 1 30 -4.085928961748635 1 31 -3.987568306010928 1 32 -3.8383879781420767 1 33 -4.014890710382513 1 34 -3.968852459016393 1 35 -3.8862021857923494 1 36 -3.772677595628415 1 37 -3.6587431693989068 1 38 -3.6091530054644805 1 39 -3.4168032786885236 1 40 -3.53620218579235 7 1 -8.02103825136612 7 2 -6.801502732240436 7 3 -6.323907103825135 7 4 -3.8707650273224035 7 5 -4.115710382513662 7 6 -3.801092896174863 7 7 -5.634699453551914 7 8 -5.446857923497268 7 9 -5.113524590163934 7 10 -3.398224043715846 7 11 -4.831284153005464 7 12 -4.824726775956282 7 13 -4.486065573770489 7 14 -5.035109289617488 7 15 -4.330327868852459 7 16 -3.621174863387977 7 17 -2.9836065573770503 7 18 -3.020628415300547 7 19 -2.806010928961748 7 20 -3.2127049180327862 7 21 -3.1140710382513657 7 22 -2.6334699453551913 7 23 -3.194262295081968 7 24 -3.090573770491803 7 25 -3.2979508196721303 7 26 -3.8588797814207645 7 27 -4.086885245901639 7 28 -4.041939890710381 7 29 -4.050136612021857 7 30 -4.085928961748635 7 31 -3.987568306010928 7 32 -3.8383879781420767 7 33 -4.014890710382513 7 34 -3.968852459016393 7 35 -3.8862021857923494 7 36 -3.772677595628415 7 37 -3.6587431693989068 7 38 -3.6091530054644805 7 39 -3.4168032786885236 7 40 -3.53620218579235 10 1 -8.02103825136612 10 2 -6.801502732240436 10 3 -6.323907103825135 10 4 -3.8707650273224035 10 5 -4.115710382513662 10 6 -3.801092896174863 10 7 -5.634699453551914 10 8 -5.446857923497268 10 9 -5.113524590163934 10 10 -3.398224043715846 10 11 -4.831284153005464 10 12 -4.824726775956282 10 13 -4.486065573770489 10 14 -5.035109289617488 10 15 -4.330327868852459 10 16 -3.621174863387977 10 17 -2.9836065573770503 10 18 -3.020628415300547 10 19 -2.806010928961748 10 20 -3.2127049180327862 10 21 -3.1140710382513657 10 22 -2.6334699453551913 10 23 -3.194262295081968 10 24 -3.090573770491803 10 25 -3.2979508196721303 10 26 -3.8588797814207645 10 27 -4.086885245901639 10 28 -4.041939890710381 10 29 -4.050136612021857 10 30 -4.085928961748635 10 31 -3.987568306010928 10 32 -3.8383879781420767 10 33 -4.014890710382513 10 34 -3.968852459016393 10 35 -3.8862021857923494 10 36 -3.772677595628415 10 37 -3.6587431693989068 10 38 -3.6091530054644805 10 39 -3.4168032786885236 10 40 -3.53620218579235 11 1 9.97896174863388 11 2 15.198497267759564 11 3 18.676092896174865 11 4 7.1292349726775965 11 5 4.884289617486338 11 6 14.198907103825137 11 7 19.365300546448086 11 8 16.55314207650273 11 9 17.886475409836066 11 10 12.601775956284154 11 11 20.168715846994537 11 12 6.175273224043718 11 13 11.513934426229511 11 14 16.964890710382512 11 15 14.669672131147541 11 16 18.378825136612022 11 17 10.016393442622949 11 18 19.979371584699454 11 19 10.193989071038253 11 20 7.787295081967214 11 21 27.885928961748633 11 22 8.366530054644809 11 23 21.80573770491803 11 24 18.9094262295082 11 25 21.70204918032787 11 26 21.141120218579236 11 27 26.91311475409836 11 28 26.95806010928962 11 29 26.94986338797814 11 30 23.914071038251365 11 31 23.012431693989072 11 32 27.161612021857923 11 33 26.985109289617487 11 34 27.031147540983607 11 35 28.11379781420765 11 36 28.227322404371584 11 37 28.341256830601093 11 38 27.39084699453552 11 39 27.583196721311477 11 40 27.463797814207652 16 1 -3.8543715846994537 16 2 -5.6348360655737695 16 3 -3.1572404371584684 16 4 -2.7040983606557365 16 5 -2.2823770491803286 16 6 -3.301092896174863 16 7 -4.968032786885247 16 8 -4.446857923497268 16 9 -4.113524590163934 16 10 -0.731557377049179 16 11 -2.4979508196721314 16 12 -2.8247267759562824 16 13 -2.319398907103822 16 14 -2.7017759562841546 16 15 -0.9969945355191259 16 16 -1.787841530054644 16 17 1.3497267759562837 16 18 -1.6872950819672141 16 19 -1.306010928961748 16 20 -1.2127049180327862 16 21 0.5525956284153013 16 22 0.5331967213114757 16 23 0.3057377049180321 16 24 -1.2572404371584698 16 25 0.5353825136612027 16 26 1.9744535519125694 16 27 1.7464480874316948 16 28 1.124726775956285 16 29 1.7831967213114766 16 30 1.7474043715846985 16 31 1.5124316939890718 16 32 1.9949453551912573 16 33 1.8184426229508208 16 34 1.864480874316941 16 35 -0.5528688524590164 16 36 1.3939890710382512 16 37 2.0079234972677593 16 38 1.5575136612021856 16 39 2.2498633879781424 16 40 1.4637978142076502 17 1 -1.0210382513661198 17 2 -0.8015027322404364 17 3 -2.6572404371584684 17 4 -0.2040983606557365 17 5 -0.44904371584699465 17 6 2.532240437158471 17 7 2.0319672131147524 17 8 2.8864754098360663 17 9 2.5531420765027324 17 10 2.601775956284154 17 11 -0.4979508196721305 17 12 -0.8247267759562824 17 13 -2.819398907103822 17 14 -0.7017759562841537 17 15 2.336338797814207 17 16 0.04549180327868996 17 17 3.0163934426229497 17 18 4.646038251366119 17 19 2.527322404371586 17 20 4.45396174863388 17 21 2.2192622950819683 17 22 2.6998633879781426 17 23 2.139071038251366 17 24 2.909426229508197 17 25 2.0353825136612036 17 26 7.807786885245902 17 27 7.579781420765027 17 28 7.624726775956285 17 29 7.616530054644809 17 30 7.580737704918031 17 31 6.345765027322406 17 32 7.828278688524589 17 33 7.651775956284153 17 34 7.697814207650273 17 35 7.780464480874317 17 36 7.893989071038251 17 37 8.00792349726776 17 38 8.057513661202186 17 39 8.249863387978142 17 40 8.130464480874316 18 1 1.7289617486338802 18 2 3.6984972677595636 18 3 -1.8239071038251353 18 4 1.6292349726775965 18 5 1.3842896174863384 18 6 2.198907103825137 18 7 5.115300546448086 18 8 5.803142076502732 18 9 5.636475409836066 18 10 2.101775956284154 18 11 3.9187158469945356 18 12 4.675273224043718 18 13 4.263934426229511 18 14 4.714890710382512 18 15 2.919672131147541 18 16 1.378825136612023 18 17 3.0163934426229497 18 18 5.979371584699453 18 19 7.443989071038252 18 20 3.5372950819672138 18 21 6.135928961748634 18 22 3.3665300546448087 18 23 2.805737704918032 18 24 2.909426229508197 18 25 3.9520491803278697 18 26 5.6411202185792355 18 27 5.913114754098361 18 28 6.708060109289619 18 29 5.949863387978143 18 30 5.164071038251365 18 31 8.512431693989072 18 32 8.411612021857923 18 33 8.485109289617487 18 34 8.781147540983607 18 35 7.863797814207651 18 36 11.977322404371584 18 37 12.091256830601093 18 38 12.140846994535519 18 39 12.333196721311477 18 40 12.21379781420765 23 1 0.4789617486338802 23 2 0.6984972677595636 23 3 2.6760928961748647 23 4 -0.37076502732240346 23 5 1.3842896174863384 23 6 5.198907103825137 23 7 3.3653005464480863 23 8 6.053142076502732 23 9 2.8864754098360663 23 10 3.101775956284154 23 11 10.668715846994536 23 12 10.675273224043718 23 13 -4.486065573770489 23 14 -5.035109289617488 23 15 -4.330327868852459 23 16 -3.621174863387977 23 17 -2.9836065573770503 23 18 -3.020628415300547 23 19 -2.806010928961748 23 20 -3.2127049180327862 23 21 -3.1140710382513657 23 22 -2.6334699453551913 23 23 -3.194262295081968 23 24 -3.090573770491803 23 25 -3.2979508196721303 23 26 -3.8588797814207645 23 27 -4.086885245901639 23 28 -4.041939890710381 23 29 -4.050136612021857 23 30 -4.085928961748635 23 31 -3.987568306010928 23 32 -3.8383879781420767 23 33 -4.014890710382513 23 34 -3.968852459016393 23 35 -3.8862021857923494 23 36 -3.772677595628415 23 37 -3.6587431693989068 23 38 -3.6091530054644805 23 39 -3.4168032786885236 23 40 -3.53620218579235 25 1 3.72896174863388 25 2 5.198497267759564 25 3 4.176092896174865 25 4 1.6292349726775965 25 5 0.8842896174863384 25 6 6.198907103825137 25 7 2.8653005464480863 25 8 4.803142076502732 25 9 4.386475409836066 25 10 0.851775956284154 25 11 10.918715846994536 25 12 10.675273224043718 25 13 10.513934426229511 25 14 10.464890710382512 25 15 4.169672131147541 25 16 -0.121174863387977 25 17 3.0163934426229497 25 18 -1.2706284153005472 25 19 3.193989071038252 25 20 3.5372950819672138 25 21 -3.1140710382513657 25 22 -2.6334699453551913 25 23 -3.194262295081968 25 24 -3.090573770491803 25 25 -3.2979508196721303 25 26 -3.8588797814207645 25 27 -4.086885245901639 25 28 -4.041939890710381 25 29 -4.050136612021857 25 30 -4.085928961748635 25 31 -3.987568306010928 25 32 -3.8383879781420767 25 33 -4.014890710382513 25 34 -3.968852459016393 25 35 -3.8862021857923494 25 36 -3.772677595628415 25 37 -3.6587431693989068 25 38 -3.6091530054644805 25 39 -3.4168032786885236 25 40 -3.53620218579235 26 1 0.39562841530054627 26 2 2.7818306010928975 26 3 3.7594262295081986 26 4 1.6292349726775965 26 5 1.3842896174863384 26 6 5.448907103825137 26 7 3.6153005464480863 26 8 4.303142076502732 26 9 3.4698087431694002 26 10 2.685109289617488 26 11 8.835382513661203 26 12 4.7586065573770515 26 13 6.013934426229511 26 14 2.2982240437158463 26 15 4.253005464480875 26 16 3.378825136612023 26 17 4.183060109289616 26 18 5.146038251366119 26 19 5.360655737704918 26 20 6.120628415300548 26 21 1.3025956284153004 26 22 -0.9668032786885243 26 23 -0.9442622950819679 26 24 2.242759562841531 26 25 1.5353825136612036 26 26 0.8077868852459016 26 27 1.4131147540983608 26 28 1.2080601092896188 26 29 1.6998633879781426 26 30 1.0807377049180307 26 31 0.4290983606557379 26 32 -0.5050546448087436 26 33 0.651775956284153 26 34 -0.802185792349726 26 35 0.28046448087431664 26 36 0.8939890710382512 26 37 1.0079234972677593 26 38 0.3908469945355195 26 39 0.7498633879781424 26 40 1.1304644808743163 27 1 -8.02103825136612 27 2 -6.801502732240436 27 3 -6.323907103825135 27 4 -3.8707650273224035 27 5 -4.115710382513662 27 6 -3.801092896174863 27 7 -5.634699453551914 27 8 -5.446857923497268 27 9 -5.113524590163934 27 10 -3.398224043715846 27 11 -4.831284153005464 27 12 -4.824726775956282 27 13 -4.486065573770489 27 14 -5.035109289617488 27 15 -4.330327868852459 27 16 -3.621174863387977 27 17 -2.9836065573770503 27 18 -3.020628415300547 27 19 -2.806010928961748 27 20 -3.2127049180327862 27 21 -3.1140710382513657 27 22 -2.6334699453551913 27 23 -3.194262295081968 27 24 -3.090573770491803 27 25 -3.2979508196721303 27 26 -3.8588797814207645 27 27 -4.086885245901639 27 28 -4.041939890710381 27 29 -4.050136612021857 27 30 -4.085928961748635 27 31 -3.987568306010928 27 32 -3.8383879781420767 27 33 -4.014890710382513 27 34 -3.968852459016393 27 35 -3.8862021857923494 27 36 -3.772677595628415 27 37 -3.6587431693989068 27 38 -3.6091530054644805 27 39 -3.4168032786885236 27 40 -3.53620218579235 28 1 -4.687704918032787 28 2 -1.1348360655737704 28 3 -0.3239071038251353 28 4 2.1292349726775965 28 5 -1.1157103825136616 28 6 0.532240437158471 28 7 0.03196721311475237 28 8 2.8864754098360663 28 9 0.21980874316940024 28 10 2.935109289617488 28 11 1.8353825136612016 28 12 -2.4913934426229494 28 13 4.180601092896177 28 14 -4.035109289617488 28 15 -2.330327868852459 28 16 -0.28784153005464397 28 17 4.683060109289616 28 18 4.646038251366119 28 19 -0.8060109289617481 28 20 4.45396174863388 28 21 7.219262295081968 28 22 4.699863387978143 28 23 7.139071038251366 28 24 7.242759562841531 28 25 7.035382513661204 28 26 6.474453551912569 28 27 3.579781420765027 28 28 6.291393442622953 28 29 6.2831967213114766 28 30 6.2474043715846985 28 31 6.345765027322406 28 32 5.161612021857923 28 33 6.318442622950821 28 34 6.031147540983607 28 35 6.4471311475409845 28 36 6.560655737704919 28 37 6.674590163934427 28 38 6.7241803278688534 28 39 5.583196721311476 28 40 3.797131147540984 30 1 13.97896174863388 30 2 15.198497267759564 30 3 19.676092896174865 30 4 -3.8707650273224035 30 5 -4.115710382513662 30 6 -3.801092896174863 30 7 -5.634699453551914 30 8 -5.446857923497268 30 9 -5.113524590163934 30 10 -3.398224043715846 30 11 -4.831284153005464 30 12 -4.824726775956282 30 13 -4.486065573770489 30 14 -5.035109289617488 30 15 -4.330327868852459 30 16 -3.621174863387977 30 17 -2.9836065573770503 30 18 -3.020628415300547 30 19 -2.806010928961748 30 20 -3.2127049180327862 30 21 -3.1140710382513657 30 22 -2.6334699453551913 30 23 -3.194262295081968 30 24 -3.090573770491803 30 25 -3.2979508196721303 30 26 -3.8588797814207645 30 27 -4.086885245901639 30 28 -4.041939890710381 30 29 -4.050136612021857 30 30 -4.085928961748635 30 31 -3.987568306010928 30 32 -3.8383879781420767 30 33 -4.014890710382513 30 34 -3.968852459016393 30 35 -3.8862021857923494 30 36 -3.772677595628415 30 37 -3.6587431693989068 30 38 -3.6091530054644805 30 39 -3.4168032786885236 30 40 -3.53620218579235 32 1 -5.02103825136612 32 2 -3.8015027322404364 32 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-3.8588797814207645 114 27 -4.086885245901639 114 28 -4.041939890710381 114 29 -4.050136612021857 114 30 -4.085928961748635 114 31 -3.987568306010928 114 32 -3.8383879781420767 114 33 -4.014890710382513 114 34 -3.968852459016393 114 35 -3.8862021857923494 114 36 -3.772677595628415 114 37 -3.6587431693989068 114 38 -3.6091530054644805 114 39 -3.4168032786885236 114 40 -3.53620218579235 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 1226.0 5 82.0 6 64.0 7 38.0 8 35.0 9 27.0 10 39.0 11 68.0 12 31.0 13 22.0 14 26.0 15 26.0 16 17.0 17 21.0 18 12.0 19 35.0 20 36.0 21 61.0 22 44.0 23 52.0 24 49.0 25 30.0 26 16.0 27 12.0 28 9.0 29 7.0 30 0.0 31 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 0.0 100.0 6 0.0 100.0 0.0 0.0 7 98.39805825242719 0.48543689320388345 0.24271844660194172 0.8737864077669903 8 97.84276126558005 0.862895493767977 0.7670182166826461 0.5273250239693192 9 95.6313010081613 1.6802688430148822 0.9121459433509362 1.7762842054728758 10 43.95973154362416 28.763183125599234 10.354745925215724 16.922339405560884 11 31.750599520383695 17.553956834532375 23.645083932853716 27.050359712230215 12 37.77564717162033 10.162991371045061 27.564717162032597 24.496644295302016 13 23.537871524448704 20.421860019175455 28.13998082454458 27.900287631831254 14 19.424460431654676 19.136690647482013 34.436450839328536 27.00239808153477 15 23.87344199424736 17.114093959731544 31.73537871524449 27.277085330776607 16 28.331735378715244 17.97698945349952 28.13998082454458 25.551294343240656 17 23.92138063279003 17.833173537871524 30.393096836049853 27.85234899328859 18 21.764141898370088 23.777564717162033 28.763183125599234 25.69511025886865 19 20.240384615384617 18.60576923076923 25.432692307692307 35.72115384615385 20 25.16778523489933 21.09300095877277 27.948226270373922 25.79098753595398 21 20.086289549376797 22.291466922339406 32.59827420901246 25.023969319271334 22 16.01150527325024 20.709491850431448 33.7967401725791 29.482262703739217 23 21.09300095877277 19.175455417066157 32.50239693192714 27.22914669223394 24 20.99712368168744 22.674976030680728 31.783317353787155 24.544582933844676 25 17.82273603082852 18.641618497109828 28.082851637764932 35.45279383429673 26 15.39568345323741 18.1294964028777 30.503597122302157 35.97122302158273 27 16.538461538461537 17.451923076923077 32.93269230769231 33.07692307692307 28 13.8328530259366 19.45244956772334 31.74831892411143 34.96637848222863 29 17.994241842610364 19.001919385796544 30.758157389635315 32.24568138195777 30 17.226487523992322 14.779270633397312 40.93090211132437 27.06333973128599 31 17.74580335731415 15.827338129496402 37.64988009592326 28.776978417266186 32 19.817658349328216 15.595009596928982 34.980806142034545 29.606525911708253 33 18.80095923261391 13.477218225419666 37.64988009592326 30.071942446043167 34 13.902205177372961 14.189837008628956 45.06232023010546 26.845637583892618 35 11.93672099712368 16.63470757430489 43.00095877277085 28.427612655800576 36 13.688760806916425 19.6926032660903 40.4418828049952 26.176753121998082 37 17.29287090558767 17.38921001926782 40.847784200385355 24.470134874759154 38 13.621103117505996 16.258992805755394 38.94484412470024 31.17505995203837 39 15.251798561151078 16.115107913669064 37.3621103117506 31.270983213429254 40 14.285714285714285 14.381591562799617 37.58389261744966 33.74880153403643 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 2.0 13 2.0 14 2.5 15 3.0 16 7.5 17 12.0 18 12.0 19 22.0 20 32.0 21 33.5 22 35.0 23 35.0 24 44.0 25 53.0 26 61.0 27 69.0 28 69.0 29 67.0 30 65.0 31 57.5 32 50.0 33 50.0 34 64.5 35 79.0 36 76.5 37 74.0 38 74.0 39 85.0 40 96.0 41 106.5 42 117.0 43 117.0 44 114.5 45 112.0 46 120.0 47 128.0 48 128.0 49 117.5 50 107.0 51 110.5 52 114.0 53 114.0 54 116.0 55 118.0 56 119.0 57 120.0 58 120.0 59 136.0 60 152.0 61 145.5 62 139.0 63 139.0 64 132.5 65 126.0 66 104.5 67 83.0 68 83.0 69 84.0 70 85.0 71 67.5 72 50.0 73 50.0 74 39.0 75 28.0 76 22.0 77 16.0 78 16.0 79 13.0 80 10.0 81 7.5 82 5.0 83 5.0 84 4.0 85 3.0 86 3.0 87 3.0 88 3.0 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 1.2464046021093003 8 0.0 9 0.14381591562799617 10 0.0 11 0.047938638542665384 12 0.0 13 0.0 14 0.047938638542665384 15 0.0 16 0.0 17 0.0 18 0.0 19 0.28763183125599234 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.4793863854266539 26 0.047938638542665384 27 0.28763183125599234 28 0.19175455417066153 29 0.09587727708533077 30 0.09587727708533077 31 0.047938638542665384 32 0.09587727708533077 33 0.047938638542665384 34 0.0 35 0.0 36 0.19175455417066153 37 0.4793863854266539 38 0.047938638542665384 39 0.047938638542665384 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 2086.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.31543624161074 #Duplication Level Percentage of deduplicated Percentage of total 1 98.42364532019704 95.78139980824545 2 0.935960591133005 1.821668264621285 3 0.3940886699507389 1.1505273250239694 4 0.1477832512315271 0.5752636625119847 5 0.04926108374384236 0.23969319271332695 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.04926108374384236 0.4314477468839885 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTCCAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.4314477468839885 No Hit ATTCCAGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.23969319271332695 No Hit ATTCCAGGGGGGCTGGTGAGATGGCCCAGTGGGTAAGAGC 4 0.19175455417066153 No Hit ATTCCAGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4 0.19175455417066153 No Hit ATTCCAGGGGCGTCCCCTTTTTTTTTTTTTTTTTTTTTTT 4 0.19175455417066153 No Hit ATTCCAGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTTT 3 0.14381591562799617 No Hit ATTCCAGGGCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3 0.14381591562799617 No Hit ATTCCAGGGGTGTCAAAGCCTAGGGGTCCCTGGGTTGGCA 3 0.14381591562799617 No Hit ATTCCAGGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTT 3 0.14381591562799617 No Hit ATTCCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT 3 0.14381591562799617 No Hit ATTCCAGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTTT 3 0.14381591562799617 No Hit ATTCCAGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTTT 3 0.14381591562799617 No Hit ATTCCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGCGCC 3 0.14381591562799617 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.04793863854266539 0.0 0.0 0.0 0.0 12 0.04793863854266539 0.0 0.0 0.0 0.0 13 0.04793863854266539 0.0 0.0 0.0 0.0 14 0.04793863854266539 0.0 0.0 0.0 0.0 15 0.04793863854266539 0.0 0.0 0.0 0.0 16 0.04793863854266539 0.0 0.0 0.0 0.0 17 0.04793863854266539 0.0 0.0 0.0 0.0 18 0.04793863854266539 0.0 0.0 0.0 0.0 19 0.04793863854266539 0.0 0.0 0.0 0.0 20 0.04793863854266539 0.0 0.0 0.0 0.0 21 0.04793863854266539 0.0 0.0 0.0 0.0 22 0.04793863854266539 0.0 0.0 0.0 0.0 23 0.04793863854266539 0.0 0.0 0.0 0.0 24 0.04793863854266539 0.0 0.0 0.0 0.0 25 0.04793863854266539 0.0 0.0 0.0 0.0 26 0.04793863854266539 0.0 0.0 0.0 0.0 27 0.04793863854266539 0.0 0.0 0.0 0.0 28 0.04793863854266539 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGGGGG 20 0.0021914598 34.0 6 AGGGGCC 20 0.0021914598 34.0 6 CAGGGGG 30 8.867741E-6 34.0 5 CAGGGGC 35 5.460952E-7 34.0 5 CAGGGAT 20 0.0021914598 34.0 5 CCAGGGA 40 3.2843673E-8 34.0 4 TCCAGGG 185 0.0 32.162163 3 TTCCAGG 215 0.0 31.627907 2 ATTCCAG 215 0.0 31.627907 1 CCAGGGG 120 0.0 29.75 4 CAGGGGT 40 6.3651554E-5 25.5 5 >>END_MODULE