FastQCFastQC Report
Fri 17 Jun 2016
SRR1525843_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525843_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5530
Sequences flagged as poor quality0
Sequence length40
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT571.030741410488246No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC430.7775768535262206No Hit
ATTCCAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG350.6329113924050633No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG290.5244122965641953No Hit
ATTCCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT190.3435804701627486No Hit
ATTCCAGGGGGGGAGGTAGTGACGAAAAATAACAATACAG170.3074141048824593No Hit
ATTCCAGGGATTCGTAGGAGTAAACATAACATTCTTCCCT120.216998191681736No Hit
ATTCCAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAG110.19891500904159135No Hit
ATTCCAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC100.18083182640144665No Hit
ATTCCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.18083182640144665No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC80.14466546112115733No Hit
ATTCCAGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT80.14466546112115733No Hit
ATTCCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.12658227848101267No Hit
ATTCCAGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCA70.12658227848101267No Hit
ATTCCAGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG70.12658227848101267No Hit
ATTCCAGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGA60.108499095840868No Hit
ATTCCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA60.108499095840868No Hit
ATTCCAGGGGGAGAGGGAGCCTGAGAAACGGCTAACACAT60.108499095840868No Hit
ATTCCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG60.108499095840868No Hit
ATTCCAGGGGCTATTCCAGGGTCACAGATCCTCTTCTGAG60.108499095840868No Hit
ATTCCAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG60.108499095840868No Hit
ATTCCAGGGGGGATGGTGAGATGGCTCAGTGGGTAAGAGC60.108499095840868No Hit
ATTCCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT60.108499095840868No Hit
ATTCCAGGGAACATTTCCTGGGCCTTTCAGGAATACCACG60.108499095840868No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGAA200.002412973234.38889322
CTGAAGG200.002412973234.38889324
TGAAGGA200.002412973234.38889325
TCTGAAG200.002412973234.38889323
AGGGGTT200.002526717734.0733956
AGGGGGC200.002526717734.0733956
AGGGGGA251.7540198E-434.0733956
AGGGGAT358.3690793E-734.0733956
AGGGATT200.002526717734.0733956
AGGGATC251.7540198E-434.0733956
AGGGACT200.002526717734.0733956
CAGGGTA251.7540198E-434.0733955
CAGGGGA750.034.0733955
CAGGGAT650.034.0733955
CAGGGAG251.7540198E-434.0733955
CAGGGAC251.7540198E-434.0733955
CCAGGGC251.7540198E-434.0733954
CCAGGGA1450.034.0733954
CAGGGGT601.8189894E-1234.073395
CAGGGGC301.2138464E-534.073395
Produced by FastQC (version 0.11.5)