FastQCFastQC Report
Fri 17 Jun 2016
SRR1525842_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525842_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6569
Sequences flagged as poor quality0
Sequence length56
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT1342.0398843050692648No Hit
CTGAAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC751.141726290150708No Hit
CTGAAAGGGACTTGAAACTAGAGAATCTGTTTGCCAAACTTCTTGATCTTGGGAGC330.5023595676663115No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC280.4262444816562643No Hit
CTGAAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT230.3501293956462171No Hit
CTGAAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA220.3349063784442077No Hit
CTGAAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC180.2740143096361699No Hit
CTGAAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT130.1978992236261227No Hit
CTGAAAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA130.1978992236261227No Hit
CTGAAAGGGATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCC120.18267620642411325No Hit
CTGAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.16745318922210384No Hit
CTGAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.15223017202009437No Hit
CTGAAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC90.13700715481808495No Hit
CTGAAAGGGGAGCCCACCACATATTCACAGTAGGATTAGATGTAGACACACGAGCT90.13700715481808495No Hit
CTGAAAGGGGAACGAGTGTTCGTACAGGGCTATGAGAAGGGCCAGCCAGATGAGGA90.13700715481808495No Hit
CTGAAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT80.1217841376160755No Hit
CTGAAAGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT80.1217841376160755No Hit
CTGAAAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATG80.1217841376160755No Hit
CTGAAAGGGAGCCACATTATTGACAGTCTTGCATTTTGTTTTGTCTCGTGATGGTG80.1217841376160755No Hit
CTGAAAGGGACTTGAAACTAGAGAATCTGTTTGCCAAACTCCTTGATCTTGGGAGC80.1217841376160755No Hit
CTGAAAGGGACTTGAAACTAGAGAATCTGTTTGCCAAACCTCTTGATCTTGGGAGC70.10656112041406607No Hit
CTGAAAGGGGTTGCTGAGACTCCTTGGATGGAGCCACCCTTCCCCCTGCCCTGAAC70.10656112041406607No Hit
CTGAAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC70.10656112041406607No Hit
CTGAAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG70.10656112041406607No Hit
CTGAAAGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTATCACT70.10656112041406607No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCAATC251.6803107E-550.87500440
TCAATCC251.6803107E-550.87500441
TTTTTGT458.185452E-1150.87534
AATCCGC251.8451217E-550.0923143
CAATCCG251.8451217E-550.0923142
TCCGCCC459.458745E-1150.0923145
ACAGATC700.050.09230812
CGCCCAA354.2218744E-850.09230847
CCGCCCA402.0118023E-950.09230846
ATCCGCC354.2218744E-850.09230844
TCACAGA700.050.09230810
CACAGAT700.050.09230811
AGGGGGG505.456968E-1249.7099276
AGGGGAA251.932417E-549.7099276
GGGTCAC900.049.7099277
AAGGGGT900.049.7099275
AAGGGAC451.03682396E-1049.7099275
AGGGATT451.03682396E-1049.7099276
GAGTTTT505.456968E-1249.70992731
GATGAGT505.456968E-1249.70992728
Produced by FastQC (version 0.11.5)