##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525842_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6569
Sequences flagged as poor quality	0
Sequence length	56
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.03318617750038	39.0	37.0	40.0	33.0	40.0
2	36.060587608464	39.0	36.0	40.0	29.0	40.0
3	35.64880499314964	38.0	35.0	40.0	28.0	40.0
4	34.40356218602527	38.0	35.0	40.0	17.0	40.0
5	34.15786268838484	39.0	33.0	40.0	17.0	40.0
6	32.25787791140204	38.0	29.0	40.0	12.0	40.0
7	34.90516060283148	38.0	34.0	39.0	25.0	40.0
8	35.1030598264576	38.0	34.0	39.0	27.0	40.0
9	35.0030446034404	38.0	34.0	39.0	27.0	40.0
10	34.1015375247374	38.0	33.0	39.0	22.0	40.0
11	33.93956462170802	38.0	33.0	39.0	22.0	40.0
12	33.027553661135634	38.0	30.0	39.0	19.0	40.0
13	32.18450296848835	37.0	28.0	39.0	16.0	40.0
14	32.97944892677729	38.0	30.0	39.0	18.0	40.0
15	31.82006393667225	36.0	28.0	39.0	14.0	40.0
16	32.6765108844573	37.0	30.0	39.0	19.0	40.0
17	31.37281169127721	36.0	27.0	39.0	14.0	40.0
18	30.801187395341756	35.0	25.0	39.0	13.0	40.0
19	32.08083422134267	36.0	29.0	39.0	17.0	40.0
20	32.769980210077634	37.0	30.0	39.0	20.0	40.0
21	33.364286801644084	38.0	31.0	39.0	19.0	40.0
22	32.554574516669206	38.0	30.0	39.0	16.0	40.0
23	33.42152534632364	38.0	32.0	39.0	19.0	40.0
24	32.7098492921297	38.0	30.0	39.0	17.0	40.0
25	33.30339473283605	38.0	31.0	39.0	19.0	40.0
26	33.461257421220886	38.0	33.0	39.0	17.0	40.0
27	33.862688384837874	38.0	33.0	39.0	20.0	40.0
28	34.042319987821585	38.0	33.0	39.0	22.0	40.0
29	33.34617141117369	38.0	32.0	39.0	17.0	40.0
30	33.14370528238697	38.0	32.0	39.0	17.0	40.0
31	34.137768305678186	38.0	33.0	39.0	22.0	40.0
32	33.93682447861166	38.0	33.0	39.0	20.0	40.0
33	33.81884609529609	38.0	33.0	39.0	20.0	40.0
34	33.66996498706043	38.0	33.0	39.0	19.0	40.0
35	33.83346019181002	38.0	33.0	39.0	20.0	40.0
36	33.23641345714721	38.0	32.0	39.0	20.0	40.0
37	32.98386360176587	37.0	32.0	39.0	19.0	40.0
38	32.39655959811235	37.0	31.0	39.0	12.0	40.0
39	31.282843659613334	36.0	29.0	39.0	4.0	40.0
40	29.102146445425483	35.0	23.0	38.0	4.0	40.0
41	27.03303394732836	32.0	18.0	38.0	4.0	40.0
42	28.108540112650328	33.0	23.0	38.0	4.0	40.0
43	28.327903790531284	33.0	23.0	38.0	4.0	40.0
44	27.434769371289388	33.0	20.0	38.0	4.0	40.0
45	28.350586086162277	33.0	24.0	38.0	4.0	40.0
46	26.553052214949002	31.0	18.0	38.0	4.0	40.0
47	25.518648196072462	30.0	14.0	37.0	4.0	40.0
48	26.107778961790228	30.0	18.0	37.0	4.0	40.0
49	25.2568123001979	30.0	13.0	37.0	4.0	40.0
50	26.11478154970315	31.0	18.0	37.0	4.0	40.0
51	26.410412543766174	32.0	17.0	38.0	4.0	40.0
52	26.139747297914447	32.0	16.0	38.0	4.0	40.0
53	26.021616684426853	32.0	11.0	38.0	4.0	40.0
54	25.748820216166845	32.0	4.0	37.0	4.0	40.0
55	25.298675597503426	31.0	4.0	37.0	4.0	40.0
56	24.485614248744103	31.0	4.0	38.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	2.313852160910983
1	2	3.1284089859720083
1	3	2.9585195150321155
1	4	3.74458673912455
1	5	5.5594480682715925
1	6	8.669455497816834
1	7	4.987331529348339
1	8	4.3727300477300375
1	9	4.88533760823676
1	10	5.648806842084149
1	11	-0.871480946691026
1	12	-9.453002109934893
1	13	-20.314875530631834
1	14	-10.390151281958012
1	15	1.083997538409303
1	16	0.3464143466244458
1	17	-6.151107296065273
1	18	-4.396189874551222
1	19	-0.5171865016402819
1	20	-1.2218312033438181
1	21	3.866573109430238
1	22	4.481353193958242
1	23	4.990510283367442
1	24	5.290649779725406
1	25	3.670615192463938
1	26	2.896143958748997
1	27	4.155921949199254
1	28	3.772666036111424
1	29	4.455437699555354
1	30	4.897743246482747
1	31	1.8743024482520312
1	32	4.918401812939628
1	33	3.6448481024111246
1	34	4.943104118104106
1	35	5.562621528797997
1	36	3.6314745245417583
1	37	2.74362005874611
1	38	5.911588155075549
1	39	7.538162514423018
1	40	-12.986057290048882
1	41	-4.077542928593349
1	42	-5.076408465484086
1	43	-11.893819182684727
1	44	-18.09035320421875
1	45	-6.685758499203882
1	46	-7.621458233643107
1	47	-9.467991835428808
1	48	-8.958267153015045
1	49	-13.80783611813024
1	50	-6.3772672239058785
1	51	0.05199483056626164
1	52	1.2344328640547175
1	53	0.6192104114373151
1	54	-0.351405760439377
1	55	-1.8532386147932414
1	56	2.2968608655583402
2	1	3.6300914771503017
2	2	4.81216966973269
2	3	5.197835754348354
2	4	6.45398844852626
2	5	7.354319863143388
2	6	9.609626437987778
2	7	5.850579392596202
2	8	5.612046287046276
2	9	5.936619659518811
2	10	7.1103453036226085
2	11	5.709715634505557
2	12	1.8803312233984393
2	13	4.616748400992098
2	14	7.139763247956516
2	15	7.750664205075971
2	16	6.466072466282569
2	17	8.224961080003101
2	18	7.868767390406042
2	19	7.508454524000744
2	20	6.649963668451054
2	21	8.131530374387506
2	22	9.276224988830037
2	23	8.152903445760607
2	24	8.803470292545917
2	25	6.9954015172502615
2	26	7.1354601980652355
2	27	6.890964684241986
2	28	6.66155492500031
2	29	7.59218983630749
2	30	8.085777434516942
2	31	6.763191337140917
2	32	6.884213778751594
2	33	6.482454940017959
2	34	7.1311383061383005
2	35	7.049801015977486
2	36	8.007542900610133
2	37	7.9231072382332925
2	38	9.091075334562731
2	39	9.307393283653784
2	40	7.962660658669066
2	41	12.666046814996399
2	42	10.359488970413349
2	43	9.832676543810997
2	44	-0.9023190161845633
2	45	9.886891073445689
2	46	9.173413561228685
2	47	4.83115346371649
2	48	9.40925421450632
2	49	-0.3462976565917799
2	50	9.195382348743692
2	51	13.086182864754292
2	52	10.123321752943607
2	53	11.396988189215094
2	54	12.964833555799942
2	55	12.351889590334967
2	56	11.519083087780562
3	1	2.8916299386887587
3	2	4.450631208194231
3	3	3.736297292809894
3	4	5.800142294680107
3	5	6.792781401604927
3	6	9.225011053372391
3	7	5.242887084903899
3	8	5.2505078255078175
3	9	5.652004274903426
3	10	6.148806842084149
3	11	5.017407942197863
3	12	6.580331223398442
3	13	6.862902247145943
3	14	5.732070940264208
3	15	5.250664205075971
3	16	6.435303235513338
3	17	7.67111492615695
3	18	9.81492123655989
3	19	7.716146831693049
3	20	7.011502129989513
3	21	6.877684220541351
3	22	7.614686527291575
3	23	5.668288061145219
3	24	6.657316446392073
3	25	7.626170748019494
3	26	5.773921736526777
3	27	4.144810838088141
3	28	5.16155492500031
3	29	5.999882143999798
3	30	7.0088543575938616
3	31	4.96319133714092
3	32	4.007290701828516
3	33	5.067070324633349
3	34	6.55421522921522
3	35	5.4959548621313346
3	36	6.753696746763978
3	37	7.176953392079447
3	38	7.144921488408883
3	39	8.73816251442302
3	40	9.747276043284451
3	41	11.489123738073317
3	42	9.89025820118258
3	43	11.317291928426386
3	44	11.920757906892359
3	45	10.925352611907229
3	46	12.111875099690224
3	47	14.654230386793412
3	48	12.686177291429402
3	49	13.092163881869759
3	50	13.333843887205234
3	51	10.46310594167737
3	52	9.82332175294361
3	53	10.19698818921509
3	54	8.726372017338402
3	55	7.513428051873426
3	56	6.019083087780562
4	1	3.6370844841433083
4	2	4.8960857536487765
4	3	5.281751838264441
4	4	6.300142294680107
4	5	7.165508674332202
4	6	9.225011053372391
4	7	5.451977993994802
4	8	5.514144189144183
4	9	5.9156406385397915
4	10	6.376079569356875
4	11	6.92649885128877
4	12	8.153058496125713
4	13	8.281084065327764
4	14	8.195707303900576
4	15	8.568846023257791
4	16	8.053485053695155
4	17	10.162024017066042
4	18	10.714921236559888
4	19	8.81614683169305
4	20	8.293320311807694
4	21	8.34132058417772
4	22	8.723777436382484
4	23	8.313742606599767
4	24	9.13913462821025
4	25	8.498898020746768
4	26	7.037558100163142
4	27	6.8629926562699595
4	28	7.025191288636677
4	29	7.6271548712725235
4	30	7.372490721230228
4	31	6.21773679168637
4	32	6.35274524728306
4	33	6.412524870087893
4	34	6.690578865578853
4	35	6.1686821348586065
4	36	7.762787655854886
4	37	7.622407937533993
4	38	8.972194215681611
4	39	8.356344332604838
4	40	2.892730588738999
4	41	11.225487374436952
4	42	10.499349110273489
4	43	11.671837382971837
4	44	13.02075790689236
4	45	12.152625339179956
4	46	11.984602372417498
4	47	14.29968493224796
4	48	14.30435910961122
4	49	12.646709336415213
4	50	12.188389341750685
4	51	11.617651396222826
4	52	12.39604902567088
4	53	12.033351825578727
4	54	12.608190199156581
4	55	12.631609870055247
4	56	12.519083087780562
5	1	3.6370844841433083
5	2	4.441540299103323
5	3	5.281751838264441
5	4	5.93650593104374
5	5	7.256417765241295
5	6	9.679556507917844
5	7	5.270159812176622
5	8	5.423235098235089
5	9	5.9156406385397915
5	10	6.648806842084149
5	11	6.199226124016043
5	12	6.880331223398439
5	13	7.826538610782311
5	14	6.65025275844603
5	15	8.75066420507597
5	16	8.144394144604242
5	17	10.343842198884222
5	18	10.442193963832615
5	19	8.088874104420324
5	20	7.747865766353147
5	21	7.977684220541352
5	22	6.632868345473398
5	23	6.7682880611452205
5	24	8.411861900937524
5	25	7.407988929837675
5	26	7.583012645617682
5	27	7.044810838088139
5	28	6.207009470454857
5	29	6.899882143999797
5	30	7.554308903048408
5	31	5.4904640644136435
5	32	6.080017974555787
5	33	5.503433960996979
5	34	5.5087606837606735
5	35	5.986863953040427
5	36	6.6718785649458
5	37	6.440589755715813
5	38	6.154012397499791
5	39	9.083617059877565
5	40	10.892730588739
5	41	13.680032828982412
5	42	12.590258201182582
5	43	12.490019201153657
5	44	12.293485179619633
5	45	11.879898066452682
5	46	11.257329645144772
5	47	13.754230386793413
5	48	12.66799547324758
5	49	12.555800245506127
5	50	11.097480250841599
5	51	12.344924123495552
5	52	12.123321752943607
5	53	12.94244273466964
5	54	11.880917471883855
5	55	12.26797350641888
5	56	10.882719451416929
6	1	2.191629938688763
6	2	3.0506312081942326
6	3	3.736297292809894
6	4	4.4001422946801085
6	5	5.99278140160493
6	6	7.12501105337239
6	7	3.7428870849038987
6	8	3.2505078255078175
6	9	3.852004274903422
6	10	5.448806842084146
6	11	5.81740794219786
6	12	5.780331223398438
6	13	7.462902247145944
6	14	6.232070940264208
6	15	6.850664205075972
6	16	3.235303235513335
6	17	6.271114926156951
6	18	5.7149212365598885
6	19	1.8161468316930502
6	20	3.311502129989517
6	21	2.2776842205413494
6	22	3.414686527291579
6	23	5.668288061145219
6	24	4.757316446392068
6	25	5.526170748019492
6	26	3.773921736526777
6	27	1.7448108380881422
6	28	3.0615549250003085
6	29	1.699882143999794
6	30	1.5088543575938616
6	31	5.1631913371409155
6	32	2.1072907018285107
6	33	-0.4329296753666547
6	34	0.45421522921521884
6	35	1.8959548621313331
6	36	0.8536967467639798
6	37	1.0769533920794458
6	38	0.5449214884088853
6	39	4.538162514423018
6	40	-1.852723956715547
6	41	7.18912373807332
6	42	6.790258201182585
6	43	3.217291928426384
6	44	4.52075790689236
6	45	4.0253526119072305
6	46	9.411875099690228
6	47	9.354230386793414
6	48	8.886177291429398
6	49	7.19216388186976
6	50	7.4338438872052315
6	51	7.563105941677371
6	52	7.723321752943608
6	53	6.696988189215094
6	54	6.426372017338402
6	55	5.013428051873426
6	56	4.719083087780561
7	1	3.0916299386887616
7	2	4.050631208194233
7	3	3.736297292809894
7	4	5.50014229468011
7	5	6.292781401604927
7	6	9.12501105337239
7	7	5.042887084903896
7	8	5.350507825507819
7	9	4.7520042749034275
7	10	6.24880684208415
7	11	4.517407942197863
7	12	7.180331223398436
7	13	6.862902247145943
7	14	6.532070940264212
7	15	7.050664205075968
7	16	6.3353032355133365
7	17	9.771114926156951
7	18	10.614921236559887
7	19	7.416146831693052
7	20	6.7115021299895155
7	21	7.7776842205413494
7	22	8.614686527291575
7	23	6.2682880611452205
7	24	8.257316446392068
7	25	7.126170748019494
7	26	7.073921736526774
7	27	4.8448108380881365
7	28	6.261554925000311
7	29	5.899882143999797
7	30	7.0088543575938616
7	31	3.6631913371409155
7	32	4.707290701828512
7	33	4.667070324633343
7	34	6.454215229215219
7	35	4.595954862131336
7	36	4.953696746763981
7	37	6.876953392079443
7	38	6.544921488408882
7	39	6.738162514423021
7	40	7.1472760432844495
7	41	10.989123738073317
7	42	11.49025820118258
7	43	9.917291928426387
7	44	10.420757906892359
7	45	10.125352611907232
7	46	10.911875099690228
7	47	14.254230386793413
7	48	11.9861772914294
7	49	13.992163881869757
7	50	12.933843887205235
7	51	10.563105941677371
7	52	11.923321752943604
7	53	12.596988189215097
7	54	8.6263720173384
7	55	6.413428051873428
7	56	10.519083087780562
8	1	0.691629938688763
8	2	2.950631208194231
8	3	3.3362972928098955
8	4	5.800142294680107
8	5	5.292781401604927
8	6	7.725011053372391
8	7	2.842887084903893
8	8	3.450507825507813
8	9	3.452004274903423
8	10	4.948806842084146
8	11	4.31740794219786
8	12	6.180331223398436
8	13	5.362902247145943
8	14	6.3320709402642095
8	15	5.1506642050759694
8	16	5.135303235513334
8	17	2.871114926156949
8	18	6.7149212365598885
8	19	4.616146831693047
8	20	6.011502129989513
8	21	6.577684220541354
8	22	7.214686527291576
8	23	5.968288061145223
8	24	6.757316446392068
8	25	5.826170748019496
8	26	5.473921736526773
8	27	2.6448108380881408
8	28	2.2615549250003113
8	29	3.699882143999794
8	30	4.0088543575938616
8	31	0.5631913371409141
8	32	2.707290701828512
8	33	-0.3329296753666533
8	34	1.5542152292152203
8	35	2.7959548621313317
8	36	3.453696746763981
8	37	2.7769533920794487
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116	49	1.1921638818697602
116	50	2.608843887205232
116	51	4.038105941677372
116	52	3.998321752943607
116	53	5.646988189215094
116	54	4.301372017338402
116	55	5.1884280518734265
116	56	7.144083087780562
117	1	2.0916299386887616
117	2	3.150631208194227
117	3	0.9362972928098969
117	4	4.300142294680107
117	5	5.292781401604927
117	6	8.025011053372388
117	7	2.542887084903896
117	8	1.7505078255078175
117	9	1.152004274903426
117	10	5.24880684208415
117	11	1.0174079421978632
117	12	-14.51966877660156
117	13	-9.737097752854055
117	14	-0.16792905973579053
117	15	-5.249335794924029
117	16	-9.164696764486663
117	17	-3.128885073843051
117	18	-6.485078763440111
117	19	-3.9838531683069505
117	20	-3.4884978700104874
117	21	2.377684220541351
117	22	6.01468652729158
117	23	4.368288061145222
117	24	5.157316446392073
117	25	2.426170748019498
117	26	6.073921736526774
117	27	3.444810838088138
117	28	4.66155492500031
117	29	4.299882143999795
117	30	5.40885435759386
117	31	2.563191337140914
117	32	4.607290701828511
117	33	0.867070324633346
117	34	2.0542152292152203
117	35	3.2959548621313317
117	36	5.253696746763978
117	37	4.276953392079449
117	38	5.244921488408885
117	39	8.338162514423015
117	40	8.547276043284455
117	41	6.789123738073318
117	42	5.790258201182581
117	43	-10.982708071573615
117	44	-0.17924209310763928
117	45	8.625352611907232
117	46	-4.688124900309774
117	47	-6.845769613206588
117	48	-1.9138227085705992
117	49	-8.20783611813024
117	50	-7.266156112794768
117	51	5.963105941677373
117	52	4.523321752943605
117	53	5.396988189215094
117	54	3.626372017338401
117	55	3.413428051873428
117	56	6.319083087780562
118	1	2.691629938688763
118	2	3.5506312081942326
118	3	3.1362972928098927
118	4	4.50014229468011
118	5	5.292781401604927
118	6	8.62501105337239
118	7	2.542887084903896
118	8	4.350507825507819
118	9	4.352004274903422
118	10	5.448806842084146
118	11	3.8174079421978604
118	12	0.68033122339844
118	13	0.4629022471459443
118	14	1.432070940264211
118	15	-3.6493357949240277
118	16	0.2353032355133351
118	17	2.071114926156948
118	18	-1.4850787634401108
118	19	0.41614683169305167
118	20	-0.888497870010486
118	21	6.377684220541351
118	22	8.414686527291579
118	23	6.968288061145223
118	24	7.357316446392069
118	25	5.626170748019494
118	26	6.073921736526774
118	27	4.444810838088138
118	28	4.861554925000313
118	29	7.0998821439998
118	30	7.0088543575938616
118	31	2.763191337140917
118	32	0.6072907018285107
118	33	4.867070324633346
118	34	5.454215229215219
118	35	6.095954862131336
118	36	4.85369674676398
118	37	7.476953392079444
118	38	7.044921488408882
118	39	9.13816251442302
118	40	11.14727604328445
118	41	8.18912373807332
118	42	3.1902582011825835
118	43	5.017291928426385
118	44	-1.1792420931076393
118	45	9.825352611907228
118	46	0.9118750996902243
118	47	0.754230386793413
118	48	2.4861772914293994
118	49	5.3921638818697595
118	50	6.9338438872052315
118	51	11.163105941677372
118	52	10.523321752943605
118	53	8.796988189215092
118	54	11.426372017338402
118	55	12.813428051873426
118	56	13.319083087780559
119	1	0.5916299386887616
119	2	2.6839645415275655
119	3	1.9029639594765584
119	4	4.800142294680107
119	5	5.226114734938264
119	6	7.558344386705727
119	7	2.70955375157056
119	8	1.9838411588411518
119	9	2.0520042749034246
119	10	4.482140175417484
119	11	3.350741275531199
119	12	-5.286335443268229
119	13	4.629568913812609
119	14	-2.0012623930691227
119	15	-7.582669128257361
119	16	-1.098030097819997
119	17	3.2377815928236124
119	18	0.7149212365598885
119	19	0.4828134983597181
119	20	1.7781687966561783
119	21	4.644350887208017
119	22	5.8146865272915775
119	23	5.934954727811885
119	24	3.9573164463920705
119	25	6.392837414686159
119	26	4.34058840319344
119	27	5.211477504754804
119	28	4.328221591666974
119	29	6.2332154773331325
119	30	5.342187690927197
119	31	0.5965246704742526
119	32	3.8072907018285136
119	33	3.867070324633346
119	34	5.387548562548556
119	35	5.062621528797997
119	36	5.85369674676398
119	37	5.9102867254127815
119	38	7.07825482174222
119	39	7.704829181089682
119	40	10.013942709951117
119	41	10.589123738073319
119	42	8.923591534515918
119	43	2.383958595093052
119	44	1.52075790689236
119	45	9.092019278573893
119	46	3.711875099690225
119	47	-3.245769613206587
119	48	-7.1804893752372685
119	49	3.858830548536428
119	50	7.067177220538564
119	51	11.163105941677372
119	52	9.789988419610271
119	53	8.563654855881762
119	54	8.926372017338402
119	55	9.813428051873426
119	56	12.519083087780562
120	1	3.0916299386887616
120	2	4.35063120819423
120	3	4.736297292809894
120	4	6.300142294680107
120	5	6.892781401604928
120	6	9.225011053372391
120	7	5.542887084903896
120	8	5.150507825507816
120	9	5.552004274903425
120	10	6.648806842084149
120	11	0.7674079421978632
120	12	-11.11966877660156
120	13	-3.5370977528540557
120	14	-5.9179290597357905
120	15	2.500664205075971
120	16	-3.264696764486665
120	17	2.071114926156948
120	18	-9.035078763440112
120	19	-1.1838531683069498
120	20	-2.638497870010486
120	21	5.727684220541352
120	22	6.5646865272915775
120	23	6.5182880611452205
120	24	6.2073164463920705
120	25	6.976170748019495
120	26	7.673921736526776
120	27	5.794810838088139
120	28	6.41155492500031
120	29	7.899882143999797
120	30	8.008854357593862
120	31	5.513191337140917
120	32	4.807290701828514
120	33	7.117070324633346
120	34	7.30421522921522
120	35	7.145954862131333
120	36	7.10369674676398
120	37	7.576953392079446
120	38	8.494921488408885
120	39	9.788162514423018
120	40	11.847276043284452
120	41	-3.4108762619266813
120	42	-2.159741798817418
120	43	-15.782708071573616
120	44	-15.47924209310764
120	45	-3.574647388092771
120	46	-11.538124900309775
120	47	-10.745769613206587
120	48	-9.2638227085706
120	49	-16.55783611813024
120	50	-6.016156112794768
120	51	1.6631059416773724
120	52	1.3733217529436068
120	53	2.6469881892150937
120	54	1.1763720173384016
120	55	2.0634280518734265
120	56	6.269083087780562
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	202.0
5	31.0
6	9.0
7	8.0
8	8.0
9	7.0
10	13.0
11	13.0
12	12.0
13	10.0
14	7.0
15	30.0
16	31.0
17	39.0
18	39.0
19	55.0
20	65.0
21	107.0
22	120.0
23	147.0
24	189.0
25	205.0
26	206.0
27	212.0
28	217.0
29	200.0
30	238.0
31	297.0
32	293.0
33	371.0
34	366.0
35	454.0
36	488.0
37	555.0
38	591.0
39	732.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.3301872431116	0.5784746536763586	0.06089206880803775	0.030446034404018874
8	99.54330948393971	0.3349063784442077	0.09133810321205663	0.030446034404018874
9	99.13228801948546	0.487136550464302	0.3196833612421982	0.06089206880803775
10	41.90896635713198	35.98721266555031	16.090729182523976	6.013091794793728
11	25.361546658547724	21.15999391079312	26.716395189526565	26.762064241132595
12	26.30537372507231	26.518495965900442	25.361546658547724	21.814583650479523
13	22.2560511493378	17.399908661896788	25.391992692951742	34.95204749581367
14	16.76054193941239	38.25544222864971	25.057086314507536	19.926929517430356
15	28.978840846366143	26.21895124195032	24.85433915976694	19.94786875191659
16	22.0816824138982	41.770801584882655	21.350198110332215	14.797317890886925
17	21.24904798172125	27.235338918507235	34.668697638994665	16.846915460776845
18	20.61196529152078	24.9961942456995	23.062871061044298	31.328969401735424
19	17.79131759329779	28.194973343488194	25.3008377760853	28.71287128712871
20	20.173833485818847	28.469045440683132	36.0780725831046	15.279048490393413
21	18.769980210077637	25.742122088597956	25.681230019789925	29.80666768153448
22	17.460800730704825	27.858121479677273	37.34206119652915	17.33901659308875
23	17.171563403866646	31.16151621251332	35.40873801187395	16.25818237174608
24	14.111736946262749	33.627644999238846	24.250266402801035	28.01035165169737
25	16.0931790499391	35.931790499390985	31.668696711327648	16.306333739342264
26	31.092821216278004	34.21734491693339	18.884316415180614	15.805517451607987
27	18.48406283361293	42.8397132835138	21.244471557114533	17.43175232575873
28	25.254985538133656	34.90637844420764	21.083878824783074	18.75475719287563
29	24.073226544622425	39.588100686498855	19.664378337147216	16.674294431731504
30	21.50586800792562	30.574607529340042	30.97088858405731	16.94863587867703
31	24.177831912302068	34.91169305724726	25.289281364190014	15.621193666260657
32	23.911056960097472	37.983551629607064	21.83978068839476	16.2656107219007
33	28.292385167099038	35.14420875934686	21.486342133374027	15.077063940180071
34	18.147130647130645	29.838217338217337	33.68437118437119	18.330280830280827
35	15.959719255416541	32.54501068050046	35.74916081782118	15.746109246261824
36	21.71925011431184	31.763450693491848	29.61438805060204	16.902911141594267
37	21.956057369545317	32.17882209337809	29.157766249618554	16.70735428745804
38	22.600335724095835	32.198992827712495	29.238516709903863	15.962154738287806
39	24.21839255757206	32.270855574195515	20.481927710843372	23.02882415738905
40	19.038106945298093	26.09096496619545	28.21143208358943	26.659496004917028
41	16.833358978304354	21.234036005539313	25.450069241421758	36.48253577473458
42	16.25382262996942	36.13149847094802	23.19571865443425	24.418960244648318
43	15.438703760317946	35.96759400794864	26.16936716600428	22.424335065729135
44	15.990197580027568	37.27982845765048	27.308929391943636	19.421044570378314
45	21.731826511912033	32.57483200977397	27.916921197312156	17.77642028100183
46	16.954330227585153	36.52054376050099	22.361386894760958	24.163739117152893
47	13.821636530799877	31.780570027581977	22.92368985596077	31.47410358565737
48	23.198781416603197	33.89185072353389	22.696115765422697	20.21325209444021
49	15.988994191378783	32.635279730969124	24.121063894833384	27.254662182818713
50	14.667277167277168	36.7063492063492	25.0	23.626373626373624
51	16.776265484018964	35.51001682214406	25.17204465514605	22.54167303869093
52	17.585101511219662	43.3216302854526	22.92779728285758	16.165470920470156
53	17.435819070904646	42.61919315403423	23.94559902200489	15.999388753056234
54	17.068702290076335	35.29770992366412	28.198473282442748	19.435114503816795
55	17.62188598502216	33.012379642365886	26.287635641143208	23.078098731468746
56	15.350542233083855	33.37406445700321	30.563616923781883	20.711776386131053
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	3.0
5	5.0
6	3.0
7	1.0
8	4.5
9	8.0
10	35.5
11	63.0
12	101.0
13	138.5
14	138.0
15	123.5
16	109.0
17	95.5
18	82.0
19	80.0
20	78.0
21	75.0
22	75.5
23	79.0
24	80.0
25	81.0
26	96.0
27	111.0
28	112.0
29	117.5
30	122.0
31	128.0
32	134.0
33	157.0
34	180.0
35	184.5
36	189.0
37	194.5
38	206.5
39	213.0
40	230.5
41	248.0
42	315.5
43	383.0
44	483.5
45	584.0
46	577.0
47	484.5
48	399.0
49	354.5
50	310.0
51	309.5
52	309.0
53	292.0
54	261.5
55	248.0
56	243.5
57	239.0
58	250.5
59	262.0
60	236.5
61	211.0
62	172.5
63	116.5
64	99.0
65	80.0
66	61.0
67	55.0
68	49.0
69	42.5
70	36.0
71	25.0
72	12.5
73	11.0
74	6.0
75	1.0
76	2.0
77	3.0
78	3.5
79	2.5
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.7154818084944436
16	0.10656112041406607
17	0.06089206880803775
18	0.0
19	0.06089206880803775
20	0.16745318922210384
21	0.0
22	0.0
23	0.0
24	0.0
25	0.015223017202009437
26	0.1217841376160755
27	0.18267620642411325
28	0.0
29	0.21312224082813214
30	0.1217841376160755
31	0.015223017202009437
32	0.04566905160602831
33	0.243568275232151
34	0.25879129243416044
35	0.22834525803014158
36	0.1217841376160755
37	0.22834525803014158
38	0.243568275232151
39	0.18267620642411325
40	0.9286040493225758
41	1.0656112041406607
42	0.4414674988582737
43	0.41102146445425486
44	0.6089206880803775
45	0.3196833612421982
46	0.3349063784442077
47	0.6545897396864059
48	0.06089206880803775
49	0.41102146445425486
50	0.25879129243416044
51	0.45669051606028316
52	0.2740143096361699
53	0.380575430050236
54	0.2892373268381793
55	0.3957984472522454
56	0.3349063784442077
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	6569.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.7370984929213
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.68997240110396	75.03425178870452
2	6.752529898804048	11.173694626274926
3	1.3431462741490339	3.333840767240067
4	0.36798528058877644	1.217841376160755
5	0.2391904323827047	0.9894961181306134
6	0.14719411223551057	0.730704825696453
7	0.09199632014719411	0.5328056020703303
8	0.09199632014719411	0.6089206880803775
9	0.05519779208831647	0.41102146445425486
>10	0.18399264029438822	2.7858121479677274
>50	0.01839926402943882	1.141726290150708
>100	0.01839926402943882	2.0398843050692648
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGAAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	134	2.0398843050692648	No Hit
CTGAAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	75	1.141726290150708	No Hit
CTGAAAGGGACTTGAAACTAGAGAATCTGTTTGCCAAACTTCTTGATCTTGGGAGC	33	0.5023595676663115	No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	28	0.4262444816562643	No Hit
CTGAAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	23	0.3501293956462171	No Hit
CTGAAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	22	0.3349063784442077	No Hit
CTGAAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	18	0.2740143096361699	No Hit
CTGAAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT	13	0.1978992236261227	No Hit
CTGAAAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	13	0.1978992236261227	No Hit
CTGAAAGGGATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCC	12	0.18267620642411325	No Hit
CTGAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11	0.16745318922210384	No Hit
CTGAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10	0.15223017202009437	No Hit
CTGAAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	9	0.13700715481808495	No Hit
CTGAAAGGGGAGCCCACCACATATTCACAGTAGGATTAGATGTAGACACACGAGCT	9	0.13700715481808495	No Hit
CTGAAAGGGGAACGAGTGTTCGTACAGGGCTATGAGAAGGGCCAGCCAGATGAGGA	9	0.13700715481808495	No Hit
CTGAAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT	8	0.1217841376160755	No Hit
CTGAAAGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT	8	0.1217841376160755	No Hit
CTGAAAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATG	8	0.1217841376160755	No Hit
CTGAAAGGGAGCCACATTATTGACAGTCTTGCATTTTGTTTTGTCTCGTGATGGTG	8	0.1217841376160755	No Hit
CTGAAAGGGACTTGAAACTAGAGAATCTGTTTGCCAAACTCCTTGATCTTGGGAGC	8	0.1217841376160755	No Hit
CTGAAAGGGACTTGAAACTAGAGAATCTGTTTGCCAAACCTCTTGATCTTGGGAGC	7	0.10656112041406607	No Hit
CTGAAAGGGGTTGCTGAGACTCCTTGGATGGAGCCACCCTTCCCCCTGCCCTGAAC	7	0.10656112041406607	No Hit
CTGAAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC	7	0.10656112041406607	No Hit
CTGAAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG	7	0.10656112041406607	No Hit
CTGAAAGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTATCACT	7	0.10656112041406607	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.030446034404018878	0.0	0.0	0.0	0.0
15	0.030446034404018878	0.0	0.0	0.0	0.0
16	0.030446034404018878	0.0	0.0	0.0	0.0
17	0.030446034404018878	0.0	0.0	0.0	0.0
18	0.030446034404018878	0.0	0.0	0.0	0.0
19	0.030446034404018878	0.0	0.0	0.0	0.0
20	0.030446034404018878	0.0	0.0	0.0	0.0
21	0.030446034404018878	0.0	0.0	0.0	0.0
22	0.030446034404018878	0.0	0.0	0.0	0.0
23	0.030446034404018878	0.0	0.0	0.0	0.0
24	0.030446034404018878	0.0	0.0	0.0	0.0
25	0.030446034404018878	0.0	0.0	0.0	0.0
26	0.030446034404018878	0.0	0.0	0.0	0.0
27	0.060892068808037755	0.0	0.0	0.0	0.0
28	0.060892068808037755	0.0	0.0	0.0	0.0
29	0.060892068808037755	0.0	0.0	0.0	0.0
30	0.060892068808037755	0.0	0.0	0.0	0.0
31	0.09133810321205663	0.0	0.0	0.0	0.0
32	0.10656112041406607	0.0	0.0	0.0	0.0
33	0.10656112041406607	0.0	0.0	0.0	0.0
34	0.10656112041406607	0.0	0.0	0.0	0.0
35	0.10656112041406607	0.0	0.0	0.0	0.0
36	0.10656112041406607	0.0	0.0	0.0	0.0
37	0.10656112041406607	0.0	0.0	0.0	0.0
38	0.10656112041406607	0.0	0.0	0.0	0.0
39	0.10656112041406607	0.0	0.0	0.0	0.0
40	0.10656112041406607	0.0	0.0	0.0	0.0
41	0.10656112041406607	0.0	0.0	0.0	0.0
42	0.10656112041406607	0.0	0.0	0.0	0.0
43	0.12178413761607551	0.0	0.0	0.0	0.0
44	0.1522301720200944	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCAATC	25	1.6803107E-5	50.875004	40
TCAATCC	25	1.6803107E-5	50.875004	41
TTTTTGT	45	8.185452E-11	50.875	34
AATCCGC	25	1.8451217E-5	50.09231	43
CAATCCG	25	1.8451217E-5	50.09231	42
TCCGCCC	45	9.458745E-11	50.09231	45
ACAGATC	70	0.0	50.092308	12
CGCCCAA	35	4.2218744E-8	50.092308	47
CCGCCCA	40	2.0118023E-9	50.092308	46
ATCCGCC	35	4.2218744E-8	50.092308	44
TCACAGA	70	0.0	50.092308	10
CACAGAT	70	0.0	50.092308	11
AGGGGGG	50	5.456968E-12	49.709927	6
AGGGGAA	25	1.932417E-5	49.709927	6
GGGTCAC	90	0.0	49.709927	7
AAGGGGT	90	0.0	49.709927	5
AAGGGAC	45	1.03682396E-10	49.709927	5
AGGGATT	45	1.03682396E-10	49.709927	6
GAGTTTT	50	5.456968E-12	49.709927	31
GATGAGT	50	5.456968E-12	49.709927	28
>>END_MODULE