SRR1525838_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1525838_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28459
Sequences flagged as poor quality	0
Sequence length	56
%GC	49

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTATACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	643	2.259390702413999	No Hit
GTATACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	488	1.7147475315365965	No Hit
GTATACGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA	303	1.0646895533926	No Hit
GTATACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	185	0.6500579781439966	No Hit
GTATACGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG	169	0.5938367476018132	No Hit
GTATACGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCCCCGCGGCCAAGGAGA	165	0.5797814399662673	No Hit
GTATACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	145	0.5095049017885379	No Hit
GTATACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	97	0.34084121016198743	No Hit
GTATACGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGAGCTCGTATGCCGT	75	0.2635370181664851	Illumina Single End Adapter 2 (95% over 24bp)
GTATACGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC	66	0.23191257598650689	No Hit
GTATACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	56	0.19677430689764222	No Hit
GTATACGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGATATCGTATGCCGT	53	0.18623282617098283	No Hit
GTATACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC	49	0.17217751853543695	No Hit
GTATACGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCCCCGCGGCCAAGGAGAG	49	0.17217751853543695	No Hit
GTATACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	48	0.16866369162655048	No Hit
GTATACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	47	0.16514986471766402	No Hit
GTATACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT	46	0.16163603780877753	No Hit
GTATACGGGTCACAGATCCTCTTCTGAGATGAGGATCGGACGAGCTCGTATGCCGT	44	0.1546083839910046	Illumina Single End Adapter 1 (95% over 23bp)
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	44	0.1546083839910046	No Hit
GTATACGGGGAGAATCTGTTTGCCAAACTTCTTGATCTTGGGAGCCAGTAGACTGG	43	0.15109455708211814	No Hit
GTATACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA	36	0.12649776871991286	No Hit
GTATACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35	0.12298394181102638	No Hit
GTATACGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT	31	0.10892863417548052	No Hit
GTATACGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	30	0.10541480726659405	No Hit
GTATACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	30	0.10541480726659405	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTGAC	25	1.9750001E-5	50.256638	41
GCGCATT	40	2.413799E-9	50.256638	38
CTTAATC	15	0.00817947	50.256638	40
CTTGAAC	15	0.00817947	50.256638	41
CGCATTG	40	2.413799E-9	50.256638	39
TTAATCC	20	4.0118015E-4	50.256638	41
GCCGATT	30	9.766009E-7	50.256638	38
GCATTGA	40	2.413799E-9	50.256638	40
CCGATTG	30	9.766009E-7	50.256638	39
CGATTGA	35	4.8477887E-8	50.256638	40
AAACAAC	20	4.0470832E-4	50.167843	37
GGCGCAT	40	2.4519977E-9	50.167843	37
GGCCGAT	30	9.886462E-7	50.167843	37
CGGACGA	20	4.0470832E-4	50.167843	37
TCGGACG	20	4.0470832E-4	50.167843	36
GACACAA	15	0.008294814	50.079365	45
TTGGGCC	40	2.4920155E-9	50.079365	34
TAATCCG	25	2.038128E-5	49.9912	42
GGACTCA	15	0.008352938	49.991196	9
GAGTTTC	15	0.008352938	49.991196	9

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content