##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525838_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28459
Sequences flagged as poor quality	0
Sequence length	56
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.70750904810429	38.0	35.0	39.0	29.0	40.0
2	35.3767876594399	38.0	35.0	39.0	27.0	40.0
3	33.92505007203345	38.0	33.0	39.0	17.0	40.0
4	35.413120629677785	38.0	35.0	39.0	27.0	40.0
5	32.18166485118943	38.0	29.0	39.0	12.0	40.0
6	35.34389823957272	38.0	35.0	39.0	27.0	40.0
7	35.50195017393443	38.0	35.0	39.0	29.0	40.0
8	35.49464141396395	38.0	35.0	39.0	29.0	40.0
9	35.43202501844759	38.0	35.0	39.0	28.0	40.0
10	34.64865244738044	38.0	34.0	39.0	24.0	40.0
11	35.00706279208686	38.0	35.0	39.0	27.0	40.0
12	33.732316666081026	38.0	32.0	39.0	20.0	40.0
13	33.14392635018799	38.0	31.0	39.0	18.0	40.0
14	33.89184440774447	38.0	33.0	39.0	21.0	40.0
15	32.42401349309533	37.0	30.0	39.0	17.0	40.0
16	32.444217997821426	36.0	30.0	39.0	18.0	40.0
17	31.359394216240908	35.0	27.0	38.0	15.0	40.0
18	31.167082469517553	35.0	27.0	38.0	15.0	40.0
19	32.26817526968622	36.0	29.0	38.0	18.0	40.0
20	32.88882251660283	36.0	30.0	39.0	21.0	40.0
21	33.55778488351664	38.0	32.0	39.0	20.0	40.0
22	32.89627182964967	38.0	31.0	39.0	17.0	40.0
23	33.5522330370006	38.0	32.0	39.0	20.0	40.0
24	32.847253944270705	38.0	31.0	39.0	17.0	40.0
25	33.3010295512843	38.0	32.0	39.0	20.0	40.0
26	33.46807688253276	38.0	33.0	39.0	19.0	40.0
27	33.68206894128395	38.0	33.0	39.0	21.0	40.0
28	33.81707017112337	38.0	33.0	39.0	23.0	40.0
29	33.21469482413296	38.0	32.0	39.0	19.0	40.0
30	33.13457957061035	38.0	32.0	39.0	18.0	40.0
31	33.73660353490987	38.0	33.0	39.0	22.0	40.0
32	33.64113285779543	38.0	33.0	39.0	21.0	40.0
33	33.317649952563336	38.0	33.0	39.0	19.0	40.0
34	33.23071787483749	38.0	33.0	39.0	19.0	40.0
35	33.22962858849573	38.0	33.0	39.0	18.0	40.0
36	32.55943638216382	37.0	32.0	39.0	16.0	40.0
37	32.27028356583155	36.0	31.0	39.0	13.0	40.0
38	31.598510137390633	36.0	30.0	38.0	8.0	40.0
39	30.285182191925227	35.0	27.0	38.0	4.0	40.0
40	28.46178713236586	34.0	22.0	38.0	4.0	39.0
41	26.280754770020028	31.0	16.0	38.0	4.0	39.0
42	26.89391756562072	32.0	19.0	37.0	4.0	39.0
43	27.3710952598475	33.0	21.0	38.0	4.0	39.0
44	26.270318704100635	31.0	16.0	38.0	4.0	39.0
45	27.02076671703152	32.0	20.0	38.0	4.0	39.0
46	25.3682490600513	30.0	15.0	36.0	4.0	39.0
47	24.438701289574475	29.0	10.0	36.0	4.0	39.0
48	24.965459081485648	30.0	14.0	36.0	4.0	39.0
49	24.193225341719668	29.0	8.0	36.0	4.0	39.0
50	24.7914192346885	30.0	11.0	36.0	4.0	39.0
51	25.04641765346639	31.0	4.0	36.0	4.0	39.0
52	24.834850135282338	30.0	4.0	36.0	4.0	39.0
53	24.614181805404264	30.0	4.0	36.0	4.0	39.0
54	24.210583646649567	30.0	4.0	36.0	4.0	39.0
55	24.196422924206754	30.0	4.0	36.0	4.0	39.0
56	22.66478091289223	28.0	4.0	36.0	4.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	4.994912831950806
1	2	5.09554164830945
1	3	7.5516177075646915
1	4	5.629186359801892
1	5	9.921931338405262
1	6	5.6093428923482875
1	7	5.500184599726204
1	8	5.61991718674458
1	9	5.183733161349529
1	10	5.869334578870365
1	11	0.5075846252948324
1	12	-7.368038935894969
1	13	-19.05093551708719
1	14	-5.870472130947334
1	15	1.8420295205381443
1	16	-2.212963384381652
1	17	-5.175735820263952
1	18	-4.340830873544
1	19	-0.450770512410962
1	20	-1.244076236404716
1	21	6.451548711746458
1	22	7.486354017555179
1	23	7.0545760232030545
1	24	7.3318040976788055
1	25	6.997572228773407
1	26	6.88960315677862
1	27	7.213600795159589
1	28	5.992273544823931
1	29	6.91614487109123
1	30	6.798478986696079
1	31	5.572406152584691
1	32	5.849160999569648
1	33	5.987918110270083
1	34	6.996541934009706
1	35	6.205298196321877
1	36	5.523348269331109
1	37	6.3294364328999855
1	38	5.965232738910878
1	39	7.776292571174423
1	40	-10.29615721807889
1	41	-0.8425620141493368
1	42	-1.4873695810403298
1	43	-8.723940883118235
1	44	-15.541003694089213
1	45	-2.1618429119447455
1	46	-5.985661203348492
1	47	-10.82220814246459
1	48	-4.658531463947988
1	49	-14.101680728857012
1	50	-4.9753073629835
1	51	1.5469852012461835
1	52	0.5991911992471408
1	53	1.5167565758608355
1	54	1.3898081114539664
1	55	1.2873847301793369
1	56	5.51540080869141
2	1	5.521075622648482
2	2	5.556781958386971
2	3	7.30597817268097
2	4	5.433934421817391
2	5	9.884625136854876
2	6	5.502269248937438
2	7	5.437200103602173
2	8	5.713909434806595
2	9	5.699721533442556
2	10	5.565555509102921
2	11	3.144212532271574
2	12	-2.0574769203910925
2	13	-0.6536486953817651
2	14	3.9003612023860015
2	15	3.818773706584654
2	16	2.6751180109671857
2	17	6.1469385983407
2	18	2.318568351262204
2	19	4.78953956510842
2	20	4.084412135688307
2	21	6.776645610971261
2	22	7.4170710718187465
2	23	6.807967496071271
2	24	6.703412624810589
2	25	6.296990833424573
2	26	6.248130288561562
2	27	4.897709322291373
2	28	4.94721540528905
2	29	6.12496270054859
2	30	6.036366583595299
2	31	5.06126274173198
2	32	4.126292782515385
2	33	5.1458638467041915
2	34	4.608945034784902
2	35	4.618088893996294
2	36	6.500576951501657
2	37	6.259184494915484
2	38	6.9279265373604915
2	39	6.762726679701551
2	40	4.93930789820018
2	41	8.00239922616074
2	42	6.989859101130211
2	43	5.899605628509672
2	44	-5.781313771608591
2	45	6.68747879348161
2	46	4.3118194168065465
2	47	2.517423640481148
2	48	4.429162334501623
2	49	-3.010110961415151
2	50	4.42827790833433
2	51	8.144853418300446
2	52	7.775547788394427
2	53	7.546795335550758
2	54	6.654342995174897
2	55	6.582442869714221
2	56	7.331776777683661
3	1	5.8260756226484816
3	2	5.8834486250536315
3	3	7.9534781726809705
3	4	6.054767755150728
3	5	10.36379180352154
3	6	6.121435915604103
3	7	5.955533436935511
3	8	5.622242768139934
3	9	5.877221533442558
3	10	6.093055509102925
3	11	5.219212532271577
3	12	5.233356412942243
3	13	6.083017971284903
3	14	6.129527869052666
3	15	0.9987737065846538
3	16	6.532618010967187
3	17	7.821938598340697
3	18	8.509401684595538
3	19	7.136206231775084
3	20	6.249412135688306
3	21	7.172478944304597
3	22	7.521237738485411
3	23	7.267134162737932
3	24	7.699245958143926
3	25	7.671990833424573
3	26	6.5314636218949005
3	27	5.801042655624709
3	28	5.93971540528905
3	29	7.360796033881929
3	30	6.982199916928632
3	31	5.145429408398641
3	32	4.909626115848724
3	33	5.044197180037525
3	34	4.415611701451567
3	35	4.328088893996295
3	36	6.14474361816832
3	37	6.375017828248819
3	38	7.167093204027154
3	39	7.966060013034884
3	40	7.1043078982001795
3	41	11.349065892827408
3	42	11.01402576779688
3	43	9.36210562850967
3	44	10.652019561724742
3	45	9.99164546014828
3	46	11.83015275013988
3	47	12.17825697381448
3	48	11.543329001168289
3	49	12.357389038584849
3	50	11.759111241667664
3	51	11.646520084967115
3	52	11.532214455061098
3	53	11.345128668884094
3	54	11.069342995174896
3	55	12.499942869714218
3	56	12.252610111016992
4	1	5.261460238033095
4	2	5.877294778899788
4	3	7.878093557296356
4	4	5.800921601304573
4	5	10.32533026506
4	6	5.827589761757949
4	7	5.818610360012428
4	8	5.672242768139931
4	9	5.82952922575025
4	10	6.810747816795228
4	11	5.211520224579267
4	12	6.21335641294224
4	13	5.013017971284903
4	14	6.244912484437279
4	15	6.895696783507734
4	16	8.05492570327488
4	17	9.31040013680224
4	18	9.74324783844169
4	19	8.186975462544318
4	20	7.209412135688307
4	21	7.595555867381517
4	22	7.8673915846392575
4	23	7.383288008891782
4	24	8.272322881220845
4	25	7.72968314111688
4	26	6.754540544971817
4	27	6.577196501778555
4	28	6.528946174519817
4	29	7.309257572343466
4	30	7.124507609236328
4	31	6.203890946860184
4	32	5.959626115848721
4	33	5.700351026191374
4	34	6.025611701451567
4	35	6.464242740150141
4	36	6.316282079706781
4	37	6.40501782824882
4	38	6.426323973257922
4	39	7.730675397650273
4	40	1.1027694366617204
4	41	11.207527431288948
4	42	8.978641152412266
4	43	9.673644090048132
4	44	10.47509648480166
4	45	8.714722383225197
4	46	11.616306596293725
4	47	10.345949281506787
4	48	11.667944385783677
4	49	10.735081346277155
4	50	10.814495857052275
4	51	10.398058546505574
4	52	9.999906762753401
4	53	9.832051745807167
4	54	10.337035302867204
4	55	9.673789023560374
4	56	10.4103024187093
5	1	5.557186733759593
5	2	5.767893069498079
5	3	8.104589283792084
5	4	6.043656644039615
5	5	10.386014025743766
5	6	5.9592136933818765
5	7	5.384422325824396
5	8	5.411131657028818
5	9	6.026110422331442
5	10	6.175277731325146
5	11	5.219212532271577
5	12	5.83557863516446
5	13	6.963017971284906
5	14	6.1517500912748915
5	15	7.818773706584654
5	16	8.445951344300521
5	17	8.855271931674036
5	18	9.72273501792887
5	19	8.078428453997304
5	20	7.731634357910533
5	21	6.99470116652682
5	22	7.710126627374301
5	23	7.331578607182379
5	24	8.0770237359217
5	25	7.871990833424576
5	26	7.0870191774504505
5	27	7.003264877846934
5	28	6.293048738622382
5	29	7.298573811659704
5	30	6.5933110280397464
5	31	4.087651630620869
5	32	4.731848338070947
5	33	5.5264194022597515
5	34	4.808945034784898
5	35	4.301422227329624
5	36	6.349188062612768
5	37	6.58835116158215
5	38	7.029315426249376
5	39	7.7793933463682166
5	40	9.097641231533512
5	41	11.9579547817163
5	42	10.916247990019102
5	43	10.86210562850967
5	44	11.26313067283585
5	45	10.636089904592719
5	46	12.092374972362101
5	47	12.327145862703372
5	48	11.84999566783496
5	49	12.71072237191818
5	50	12.232444575000994
5	51	11.308742307189334
5	52	11.403325566172203
5	53	12.094017557772979
5	54	12.862676328508233
5	55	12.719942869714224
5	56	12.152610111016994
6	1	5.521075622648482
6	2	5.556781958386971
6	3	7.99347817268097
6	4	6.058934421817391
6	5	10.197125136854876
6	6	5.668935915604102
6	7	5.958033436935509
6	8	5.880576101473267
6	9	5.949721533442556
6	10	6.232222175769586
6	11	5.373379198938238
6	12	6.005023079608904
6	13	6.138017971284903
6	14	6.567027869052666
6	15	7.21460703991799
6	16	7.758451344300521
6	17	9.626105265007364
6	18	9.5685683512622
6	19	7.956206231775084
6	20	7.271912135688307
6	21	7.651645610971261
6	22	8.417071071818746
6	23	7.182967496071271
6	24	8.411745958143925
6	25	7.671990833424573
6	26	6.914796955228233
6	27	6.231042655624709
6	28	5.9263820719557145
6	29	7.24996270054859
6	30	7.182199916928635
6	31	6.227929408398644
6	32	5.563792782515385
6	33	6.4583638467041915
6	34	6.671445034784902
6	35	6.180588893996294
6	36	7.583910284834985
6	37	7.59251782824882
6	38	8.427926537360491
6	39	9.346060013034887
6	40	4.730974564866848
6	41	13.273232559494073
6	42	11.781525767796879
6	43	11.649605628509672
6	44	11.302019561724741
6	45	10.520812126814942
6	46	12.895152750139879
6	47	13.309090307147816
6	48	13.512495667834955
6	49	12.864889038584849
6	50	13.136611241667662
6	51	12.95735341830045
6	52	11.879714455061094
6	53	12.296795335550755
6	54	12.341842995174897
6	55	12.186609536380885
6	56	13.310943444350329
7	1	5.483284924974065
7	2	5.09554164830945
7	3	7.574873521518182
7	4	4.908256127243753
7	5	9.898675524451772
7	6	5.144226613278519
7	7	5.058324134609926
7	8	4.945498582093421
7	9	5.532570370651861
7	10	4.706543881195948
7	11	4.298282299713435
7	12	4.492426180384101
7	13	5.530459831750022
7	14	4.966737171378249
7	15	6.888541148445118
7	16	6.624245917943927
7	17	7.5219385983407
7	18	8.356843545060656
7	19	7.200392278286714
7	20	6.872202833362724
7	21	6.5213161536069215
7	22	7.393330761741225
7	23	5.9150411394821205
7	24	6.70389712093462
7	25	5.044083856680388
7	26	4.540765947476295
7	27	5.050810097485172
7	28	4.78297121924254
7	29	4.544051847835416
7	30	4.217083637858867
7	31	4.246824757235856
7	32	3.756137743755694
7	33	3.94140648236311
7	34	4.740727980521335
7	35	4.7169261032986185
7	36	4.941952920493904
7	37	5.375948060806959
7	38	6.75593041332948
7	39	7.729780943267443
7	40	5.936400921455995
7	41	10.366740311432057
7	42	10.977746698029435
7	43	9.95047772153293
7	44	10.738066073352648
7	45	10.37304080898549
7	46	10.898059726884064
7	47	11.968489531954017
7	48	12.085654582563642
7	49	12.037854154863922
7	50	10.396785660272311
7	51	12.64000845706014
7	52	9.738726082968068
7	53	9.958617040977114
7	54	10.971203460291175
7	55	9.868780079016545
7	56	9.980517087761179
8	1	5.4509536714289695
8	2	5.699058381151197
8	3	7.895917197071217
8	4	5.832816535638528
8	5	10.14427960839959
8	6	5.298509086335812
8	7	5.115045632057459
8	8	5.361267158383832
8	9	5.698197143198655
8	10	5.533543313980971
8	11	4.6777491176374255
8	12	5.920673486112975
8	13	6.372774068845878
8	14	6.592942503199005
8	15	8.111456633413919
8	16	7.015057035357433
8	17	7.814621525169965
8	18	9.199157782156512
8	19	7.139133061043374
8	20	7.075265794224894
8	21	6.343210651621671
8	22	7.167579201900047
8	23	6.140792699323299
8	24	5.7090020557049
8	25	6.720771321229449
8	26	6.282683134090021
8	27	5.883481680014956
8	28	5.345081258947587
8	29	6.276893594857537
8	30	6.657809673026197
8	31	5.884941603520595
8	32	5.441333432921894
8	33	5.396880106866792
8	34	4.714636091695468
8	35	5.855893772045071
8	36	6.8852314230463705
8	37	6.752578803858572
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115	41	5.446843670605187
115	42	4.019951693722806
115	43	2.980624147028191
115	44	-3.7442767345715566
115	45	3.0879417564445752
115	46	0.04793052791765717
115	47	-9.22100228544478
115	48	-0.6981524803131904
115	49	-3.933722072526262
115	50	-0.8934813509249295
115	51	2.6124460108930414
115	52	0.8773996402462814
115	53	1.782906446661869
115	54	3.714528180360084
115	55	3.371794721566072
115	56	6.093350851757734
116	1	5.090520067092925
116	2	4.723448625053635
116	3	6.521255950458748
116	4	5.204767755150726
116	5	8.502680692410426
116	6	4.759213693381881
116	7	5.173311214713287
116	8	5.20002054591771
116	9	5.609443755664778
116	10	4.5586110646584785
116	11	3.213656976716017
116	12	3.7133564129422396
116	13	4.8463513046182385
116	14	4.185083424608223
116	15	-3.2090040711931245
116	16	4.918173566522743
116	17	5.827494153896257
116	18	5.228290573484422
116	19	5.400650676219527
116	20	3.9871899134660858
116	21	4.922478944304597
116	22	5.9101266273742965
116	23	6.064911940515714
116	24	6.015912624810589
116	25	6.033101944535687
116	26	4.970352510783783
116	27	4.564375988958044
116	28	4.204159849733493
116	29	5.243018256104147
116	30	5.6544221391508565
116	31	2.80431829728753
116	32	4.265181671404278
116	33	4.2153082911486415
116	34	3.386722812562681
116	35	4.50697778288518
116	36	5.604743618168321
116	37	6.40501782824882
116	38	6.907093204027156
116	39	7.040504457479329
116	40	9.730974564866845
116	41	8.363510337271851
116	42	5.927359101130211
116	43	0.8509945173985614
116	44	1.4964640061691838
116	45	3.930534349037167
116	46	-3.1465139165267892
116	47	-8.739520803963298
116	48	-3.57778210994282
116	49	-4.933722072526262
116	50	-2.88422209166567
116	51	1.2420756405226712
116	52	0.2755477883944266
116	53	1.0884620022174225
116	54	1.2515652173971183
116	55	0.7977206474919996
116	56	4.463721222128104
117	1	3.799921776494635
117	2	3.5311409327459415
117	3	5.762708941911736
117	4	3.800921601304573
117	5	7.36379180352154
117	6	3.404512838681022
117	7	4.203225744627815
117	8	4.1914735373706975
117	9	4.175683071904096
117	10	3.560747816795228
117	11	-0.5192490061899662
117	12	-7.171258971673144
117	13	-5.160058951792021
117	14	-2.101241361716564
117	15	-4.911995524184576
117	16	-9.848920450571274
117	17	1.0988616752637768
117	18	-0.9490598538660038
117	19	0.26389853946739095
117	20	-0.02135709508092276
117	21	4.403248175073831
117	22	5.3673915846392575
117	23	4.210211085814855
117	24	4.637707496605465
117	25	5.595067756501493
117	26	5.331463621894898
117	27	4.981042655624709
117	28	3.8751000206736634
117	29	4.290026803112692
117	30	5.759122993851715
117	31	4.434660177629411
117	32	2.9788568850794874
117	33	4.7580433338836805
117	34	4.121765547605413
117	35	4.714242740150141
117	36	4.489359002783708
117	37	4.770402443633433
117	38	5.330170127104076
117	39	6.07682924380412
117	40	7.1412309751232605
117	41	4.938296662058178
117	42	5.824794998566109
117	43	-2.1148174484134046
117	44	-6.255672745967566
117	45	3.445491613994431
117	46	-1.037539557552428
117	47	-1.7309737954162898
117	48	0.16794438578367377
117	49	-0.6303032691074577
117	50	0.5068035493599687
117	51	4.455750854197884
117	52	6.576829839676478
117	53	6.312820976576397
117	54	7.18318914902105
117	55	6.866096715868068
117	56	9.583379341786223
118	1	3.831260807833665
118	2	4.056781958386971
118	3	5.73421891342171
118	4	4.362175162558131
118	5	8.36379180352154
118	6	3.981435915604102
118	7	3.7103482517503252
118	8	3.44076128665845
118	9	3.1742585704795943
118	10	4.049351805399219
118	11	0.1303236433826882
118	12	-5.231088031502203
118	13	-4.755500547233616
118	14	-3.0926943531695557
118	15	-9.551596663785716
118	16	-4.850344951995776
118	17	-0.9965799201778189
118	18	-5.632820537626685
118	19	-2.2104604348915835
118	20	-0.3276249013487309
118	21	2.3947011665268185
118	22	4.984200701448373
118	23	3.9908378664416375
118	24	3.4325792914772606
118	25	3.0053241667579087
118	26	3.961093251524524
118	27	3.8699315445135944
118	28	4.167122812696455
118	29	4.113388626474521
118	30	5.793311028039749
118	31	3.7765405195097586
118	32	2.5985150047376067
118	33	3.437530513370863
118	34	2.497833923673788
118	35	3.886607412514813
118	36	2.9195584329831377
118	37	1.4327956060265983
118	38	1.962648759582713
118	39	4.364578531553406
118	40	6.749493083385364
118	41	2.7061029298644463
118	42	3.3532850270561383
118	43	-4.056412890008847
118	44	-2.262795253090072
118	45	3.49534916385198
118	46	-1.3594768794897512
118	47	-1.0728541372966305
118	48	-2.32778210994282
118	49	-0.5263146651188535
118	50	-0.041629499073078335
118	51	3.723557122004152
118	52	2.8773996402462814
118	53	0.597721261476682
118	54	1.6774911433230457
118	55	2.001424351195702
118	56	3.797054555461436
119	1	4.217504194077051
119	2	4.6520200536250655
119	3	6.6720496012524
119	4	4.776196326579296
119	5	8.649506089235825
119	6	4.160007344175533
119	7	4.252676294078363
119	8	4.2436713395685
119	9	4.422935819156841
119	10	3.3959126519600673
119	11	2.1977839608430045
119	12	-0.2866435870577604
119	13	-0.7369820287150972
119	14	-3.727614988090192
119	15	-7.216940579129631
119	16	-4.60166770331853
119	17	1.1647957411978425
119	18	-2.124884029690179
119	19	0.3490633746322267
119	20	1.2094121356883072
119	21	4.779621801447451
119	22	5.521237738485411
119	23	5.465705591309366
119	24	4.5278173867153555
119	25	4.314847976281719
119	26	5.081463621894898
119	27	4.409614084196139
119	28	3.652572548146196
119	29	4.5207960338819255
119	30	6.325057059785781
119	31	3.2368579798272137
119	32	4.923911830134436
119	33	3.747054322894673
119	34	4.525611701451567
119	35	3.8323746082820094
119	36	3.3904579038826057
119	37	4.726446399677389
119	38	5.728521775455725
119	39	5.5960600130348865
119	40	6.56430789820018
119	41	4.8833516071131235
119	42	3.558311482082594
119	43	-1.7521800857760432
119	44	-0.8408375811324014
119	45	2.3630740315768506
119	46	-3.4441329641458367
119	47	-1.176028740471235
119	48	-5.8952424274031365
119	49	-1.6083252471294358
119	50	-0.2294601869037649
119	51	2.5436629421099717
119	52	3.6207858836325215
119	53	1.5765572403126633
119	54	1.957914423746324
119	55	3.198514298285648
119	56	2.959752968159851
120	1	4.646075622648482
120	2	4.116305767910781
120	3	6.1720496012524
120	4	4.740482040865011
120	5	8.93522037495011
120	6	3.481435915604102
120	7	3.9312477226497933
120	8	4.2436713395685
120	9	4.565792962013987
120	10	4.503055509102921
120	11	-0.5165017534427108
120	12	-7.143786444200618
120	13	-3.594124885857955
120	14	0.3080992976240964
120	15	-1.2169405791296306
120	16	-5.173096274747099
120	17	1.1290814554835578
120	18	-1.2320268868330366
120	19	0.24192051748936905
120	20	0.6022692785454495
120	21	4.636764658590312
120	22	6.414094881342557
120	23	6.037134162737935
120	24	7.099245958143925
120	25	4.850562261996004
120	26	4.545749336180613
120	27	4.338185512767563
120	28	4.688286833860481
120	29	5.7707960338819255
120	30	5.253628488357204
120	31	3.951143694112929
120	32	4.566768972991575
120	33	4.354197180037527
120	34	3.6684688443087126
120	35	2.26094603685344
120	36	4.783315046739752
120	37	5.08358925682025
120	38	5.407093204027156
120	39	5.238917155892032
120	40	7.242879326771611
120	41	-7.009505535744019
120	42	-6.584545660774548
120	43	-7.216465800061755
120	44	-14.447980438275259
120	45	-5.779783111280295
120	46	-10.551275821288693
120	47	-7.926028740471233
120	48	-8.28809957026028
120	49	-15.072610961415151
120	50	-6.1580316154751955
120	51	-1.884908486461459
120	52	-1.7006426877960479
120	53	-1.8520141882587673
120	54	-2.720657004825103
120	55	-2.3371999874286367
120	56	1.0311815395884203
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	712.0
5	20.0
6	51.0
7	61.0
8	76.0
9	108.0
10	82.0
11	66.0
12	76.0
13	59.0
14	72.0
15	102.0
16	142.0
17	158.0
18	220.0
19	280.0
20	373.0
21	495.0
22	577.0
23	714.0
24	793.0
25	858.0
26	942.0
27	939.0
28	973.0
29	1063.0
30	1129.0
31	1272.0
32	1502.0
33	1609.0
34	1853.0
35	2035.0
36	2144.0
37	2332.0
38	2444.0
39	2127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.40616325239819	0.4849081134263326	0.07027653817772936	0.03865209599775115
8	99.30074844513159	0.6184335359640184	0.045679749815524084	0.03513826908886468
9	97.1713693383464	0.7027653817772936	1.493376436276749	0.6324888435995644
10	43.824449207632036	23.39154573245722	24.849783899645104	7.934221160265645
11	25.654450261780106	24.579219227660847	16.254963280508804	33.51136723005025
12	25.605256685055693	34.22116026564531	16.999894585192735	23.173688464106256
13	16.926104220106115	19.768790189395272	19.786359323939703	43.518746266558914
14	17.305597526265856	38.276116518500295	19.723110439579745	24.6951755156541
15	37.35833068530876	18.370229142393107	20.594569784274263	23.67687038802387
16	22.23940938653542	37.51801722622605	20.35507119001582	19.887502197222712
17	19.86855054126248	20.61717981161254	37.5333895683959	21.980880078729086
18	18.911416423626974	19.884746477388525	18.647879405460486	42.555957693524014
19	18.75439336426262	18.413468297483483	21.443132292984675	41.38900604526923
20	18.000492489534597	20.00562845182397	41.967143912477574	20.02673514616386
21	20.80185530060789	16.9823254506483	22.460381601602304	39.7554376471415
22	23.019080080115252	17.822130081872167	39.03158930391089	20.127200534101693
23	20.176394110826102	22.34442531360905	38.082856038511544	19.396324537053307
24	16.64030926023546	25.08873660165173	20.246002460024602	38.02495167808821
25	18.086938187440698	23.112063815581404	36.925888182169594	21.875109814808308
26	39.76509477089707	22.534022576221123	18.09262580440975	19.60825684847206
27	22.082351700130104	39.421217342381944	19.751046098667324	18.745384858820632
28	32.95382669196711	30.996556328624642	16.768571227774267	19.28104575163399
29	28.03547546983881	32.357288660519465	19.814176110368127	19.7930597592736
30	26.321895654619603	21.885107579805936	33.23020672198003	18.562790043594433
31	30.694078369355122	28.88420312774556	19.09681953962397	21.324898963275345
32	30.02389822169115	31.324242637239053	19.77929289379349	18.872566247276307
33	34.51212217178648	28.13258735353109	18.966184594813328	18.389105879869103
34	22.239042422108785	20.894208766062313	36.743531068473864	20.123217743355042
35	19.1618873368284	24.320045037120437	39.75581436261919	16.76225326343197
36	26.193321616871707	21.41652021089631	30.04569420035149	22.344463971880494
37	27.0180871278767	21.59194876486734	29.776901963544233	21.61306214371173
38	31.099686608683403	20.969048205922743	30.76868903834642	17.16257614704743
39	32.356562423048516	21.827839729834313	20.058395187673693	25.757202659443486
40	23.06657223796034	19.780453257790366	25.93838526912181	31.214589235127477
41	19.083942382743206	18.38146597601646	24.224792450152556	38.30979919108778
42	20.867695777824768	28.48382321843942	21.67124832593219	28.97723267780362
43	17.891249955950244	28.974169221552664	27.187511012439654	25.94706981005744
44	20.10161956176564	28.25235524505134	28.55933100455171	23.086694188631313
45	25.18043868605429	22.1561102700419	28.996936943280637	23.666514100623175
46	21.541606456842775	28.911288901420363	22.091424946251717	27.455679695485145
47	18.827890556045894	26.365401588702557	19.1315092674316	35.67519858781994
48	26.90198284348193	23.10153283645057	21.46322598790606	28.533258332161438
49	16.608545563422453	28.2996935432738	22.036704357321497	33.055056535982246
50	16.453156894488632	36.35531780108398	21.067079608643628	26.12444569578377
51	18.24333850274919	33.5612575778937	22.21908924291555	25.976314676441564
52	24.18100566522397	38.10127027692741	20.67982687638552	17.037897181463105
53	19.932349106796803	38.86402875162961	21.236038194566788	19.9675839470068
54	19.004684253160992	26.88690874511323	28.052689043073997	26.05571795865178
55	21.625907393050955	23.380787934315315	29.54401296779195	25.44929170484178
56	18.69695368902976	25.52914245465751	31.95985208663497	23.81405176967776
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.0
6	3.5
7	6.0
8	39.5
9	73.0
10	115.5
11	158.0
12	174.5
13	166.0
14	141.0
15	136.0
16	131.0
17	126.5
18	122.0
19	131.0
20	140.0
21	128.5
22	124.5
23	132.0
24	156.5
25	181.0
26	177.0
27	173.0
28	196.0
29	232.5
30	246.0
31	302.5
32	359.0
33	384.5
34	410.0
35	432.5
36	455.0
37	482.0
38	578.5
39	648.0
40	720.0
41	792.0
42	1045.0
43	1298.0
44	1897.5
45	2497.0
46	2613.0
47	2306.5
48	1884.0
49	1721.0
50	1558.0
51	1598.0
52	1638.0
53	1620.5
54	1500.5
55	1398.0
56	1427.0
57	1456.0
58	1439.5
59	1423.0
60	1327.5
61	1232.0
62	1091.0
63	870.0
64	790.0
65	724.0
66	658.0
67	832.0
68	1006.0
69	819.5
70	633.0
71	438.5
72	185.0
73	126.0
74	99.0
75	72.0
76	52.0
77	32.0
78	26.5
79	13.5
80	6.0
81	3.0
82	0.0
83	0.5
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.4778804596085597
16	0.049193576724410555
17	0.024596788362205278
18	0.0
19	0.024596788362205278
20	0.11244246108436699
21	0.0
22	0.0
23	0.0
24	0.014055307635545874
25	0.007027653817772937
26	0.0773041919955023
27	0.07027653817772936
28	0.0035138269088864685
29	0.1581222108998911
30	0.052707403633297026
31	0.014055307635545874
32	0.01756913454443234
33	0.14055307635545872
34	0.18974665307986927
35	0.1335254225376858
36	0.031624442179978215
37	0.1440669032643452
38	0.2108296145331881
39	0.11244246108436699
40	0.7695280930461366
41	0.9592747461260058
42	0.2986752872553498
43	0.2881338065286904
44	0.4146315752486033
45	0.19677430689764222
46	0.30218911416423627
47	0.47085280579078675
48	0.052707403633297026
49	0.24596788362205277
50	0.1581222108998911
51	0.30570294107312274
52	0.14055307635545872
53	0.27407849889314456
54	0.23191257598650689
55	0.2846199796198039
56	0.22488492216873399
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	28459.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.5745809761411
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.1740098770214	64.71766400787097
2	6.604047642103225	9.585719807442285
3	1.8253122881766244	3.9741382339505957
4	0.7698266679577805	2.2347939140517936
5	0.4405926212840128	1.598791243543343
6	0.2759755979471289	1.2017288028391722
7	0.20819211774958848	1.057661899574827
8	0.12104192892417934	0.7027653817772936
9	0.11135857461024498	0.7273621701394989
>10	0.41154255834220976	5.608067746582803
>50	0.02420838578483587	1.2192979373836046
>100	0.02905006294180304	5.112618152429811
>500	0.004841677156967174	2.259390702413999
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	643	2.259390702413999	No Hit
GTATACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	488	1.7147475315365965	No Hit
GTATACGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA	303	1.0646895533926	No Hit
GTATACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	185	0.6500579781439966	No Hit
GTATACGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG	169	0.5938367476018132	No Hit
GTATACGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCCCCGCGGCCAAGGAGA	165	0.5797814399662673	No Hit
GTATACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	145	0.5095049017885379	No Hit
GTATACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	97	0.34084121016198743	No Hit
GTATACGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGAGCTCGTATGCCGT	75	0.2635370181664851	Illumina Single End Adapter 2 (95% over 24bp)
GTATACGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC	66	0.23191257598650689	No Hit
GTATACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	56	0.19677430689764222	No Hit
GTATACGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGATATCGTATGCCGT	53	0.18623282617098283	No Hit
GTATACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC	49	0.17217751853543695	No Hit
GTATACGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCCCCGCGGCCAAGGAGAG	49	0.17217751853543695	No Hit
GTATACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	48	0.16866369162655048	No Hit
GTATACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	47	0.16514986471766402	No Hit
GTATACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT	46	0.16163603780877753	No Hit
GTATACGGGTCACAGATCCTCTTCTGAGATGAGGATCGGACGAGCTCGTATGCCGT	44	0.1546083839910046	Illumina Single End Adapter 1 (95% over 23bp)
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	44	0.1546083839910046	No Hit
GTATACGGGGAGAATCTGTTTGCCAAACTTCTTGATCTTGGGAGCCAGTAGACTGG	43	0.15109455708211814	No Hit
GTATACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA	36	0.12649776871991286	No Hit
GTATACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35	0.12298394181102638	No Hit
GTATACGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT	31	0.10892863417548052	No Hit
GTATACGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	30	0.10541480726659405	No Hit
GTATACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	30	0.10541480726659405	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.007027653817772936	0.0	0.0	0.0	0.0
9	0.007027653817772936	0.0	0.0	0.0	0.0
10	0.007027653817772936	0.0	0.0	0.0	0.0
11	0.014055307635545872	0.0	0.0	0.0	0.0
12	0.014055307635545872	0.0	0.0	0.0	0.0
13	0.02108296145331881	0.0	0.0	0.0	0.0
14	0.045679749815524084	0.0	0.0	0.0	0.0
15	0.045679749815524084	0.0	0.0	0.0	0.0
16	0.049193576724410555	0.0	0.0	0.0	0.0
17	0.049193576724410555	0.0	0.0	0.0	0.0
18	0.049193576724410555	0.0	0.0	0.0	0.0
19	0.052707403633297026	0.0	0.0	0.0	0.0
20	0.05622123054218349	0.0	0.0	0.0	0.0
21	0.05622123054218349	0.0	0.0	0.0	0.0
22	0.05622123054218349	0.0	0.0	0.0	0.0
23	0.05622123054218349	0.0	0.0	0.0	0.0
24	0.05622123054218349	0.0	0.0	0.0	0.0
25	0.05973505745106996	0.0	0.0	0.0	0.0
26	0.0667627112688429	0.0	0.0	0.0	0.0
27	0.07379036508661584	0.0	0.0	0.0	0.0
28	0.08433184581327524	0.0	0.0	0.0	0.0
29	0.08433184581327524	0.0	0.0	0.0	0.0
30	0.09487332653993465	0.0	0.0	0.0	0.0
31	0.10541480726659405	0.0	0.0	0.0	0.0
32	0.11244246108436698	0.0	0.0	0.0	0.0
33	0.11595628799325346	0.0	0.0	0.0	0.0
34	0.11595628799325346	0.0	0.0	0.0	0.0
35	0.11595628799325346	0.0	0.0	0.0	0.0
36	0.11595628799325346	0.0	0.0	0.0	0.0
37	0.11947011490213992	0.0	0.0	0.0	0.0
38	0.12298394181102638	0.0	0.0	0.0	0.0
39	0.12649776871991286	0.0	0.0	0.0	0.0
40	0.13001159562879933	0.0	0.0	0.0	0.0
41	0.13001159562879933	0.0	0.0	0.0	0.0
42	0.13001159562879933	0.0	0.0	0.0	0.0
43	0.1335254225376858	0.0	0.0	0.0	0.0
44	0.16866369162655048	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTGAC	25	1.9750001E-5	50.256638	41
GCGCATT	40	2.413799E-9	50.256638	38
CTTAATC	15	0.00817947	50.256638	40
CTTGAAC	15	0.00817947	50.256638	41
CGCATTG	40	2.413799E-9	50.256638	39
TTAATCC	20	4.0118015E-4	50.256638	41
GCCGATT	30	9.766009E-7	50.256638	38
GCATTGA	40	2.413799E-9	50.256638	40
CCGATTG	30	9.766009E-7	50.256638	39
CGATTGA	35	4.8477887E-8	50.256638	40
AAACAAC	20	4.0470832E-4	50.167843	37
GGCGCAT	40	2.4519977E-9	50.167843	37
GGCCGAT	30	9.886462E-7	50.167843	37
CGGACGA	20	4.0470832E-4	50.167843	37
TCGGACG	20	4.0470832E-4	50.167843	36
GACACAA	15	0.008294814	50.079365	45
TTGGGCC	40	2.4920155E-9	50.079365	34
TAATCCG	25	2.038128E-5	49.9912	42
GGACTCA	15	0.008352938	49.991196	9
GAGTTTC	15	0.008352938	49.991196	9
>>END_MODULE