FastQCFastQC Report
Fri 17 Jun 2016
SRR1525837_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525837_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3655
Sequences flagged as poor quality0
Sequence length40
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACGGGGAAACTGCATTAGGTTTAAAACTGATGGCTG80.2188782489740082No Hit
GTATACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT80.2188782489740082No Hit
GTATACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT70.19151846785225718No Hit
GTATACGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.16415868673050615No Hit
GTATACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT60.16415868673050615No Hit
GTATACGGGGTATTTATATCCTCTTAAATCTTTGTTCCCT50.13679890560875513No Hit
GTATACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT50.13679890560875513No Hit
GTATACGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC40.1094391244870041No Hit
GTATACGGGGAGTCGACTCTTTTTTTTTTTTTTTTTTTTT40.1094391244870041No Hit
GTATACGGGGGGCTGGTGCGATGGCTCCGTGGGTAAGAGC40.1094391244870041No Hit
GTATACGGGATGCCACAACTAGATACATCAACATGATTTA40.1094391244870041No Hit
GTATACGGGGGGGCTGGAGAGATGGCTCCGTGGTTAAGAG40.1094391244870041No Hit
GTATACGGGCGTCGACTTTTTTTTTTTTTTTTTTTTTTTT40.1094391244870041No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG200.002500837233.8356176
CGGGGCC200.002500837233.8356176
TACGGGC251.7085389E-433.8356174
TACGGGA1750.033.8356174
GGATCTT200.002500837233.8356178
CGGGATG357.7797813E-733.8356176
CGGGATC301.1579572E-533.8356176
CGGGAAG200.002500837233.8356176
ACGGGGT301.1579572E-533.8356175
ACGGGGG357.7797813E-733.8356175
ACGGGGC453.4087861E-933.8356175
ACGGGGA200.002500837233.8356175
ACGGGAT850.033.8356175
ACGGGAG251.7085389E-433.8356175
ACGGGAC301.1579572E-533.8356175
ACGGGAA357.7797813E-733.8356175
GGGATCT200.002500837233.8356177
TACGGGG1300.033.8356134
GTATACG3700.033.3783761
TATACGG3650.033.3721162
Produced by FastQC (version 0.11.5)