FastQCFastQC Report
Fri 17 Jun 2016
SRR1525835_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525835_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421
Sequences flagged as poor quality0
Sequence length40
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT261.8296973961998593No Hit
GTATACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG261.8296973961998593No Hit
GTATACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.9148486980999296No Hit
GTATACGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG120.844475721323012No Hit
GTATACGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT90.633356790992259No Hit
GTATACGGGTCACAGATCCTCTTCTGAGATGAGGATCGGA90.633356790992259No Hit
GTATACGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT80.5629838142153413No Hit
GTATACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.49261083743842365No Hit
GTATACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.422237860661506No Hit
GTATACGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.3518648838845883No Hit
GTATACGGGATCTGTGTGTGGCTTAGGAGCCTGCTGTCTC50.3518648838845883No Hit
GTATACGGGATTGACATGGAAGAGTGGCAGCGGAATATAA40.28149190710767064No Hit
GTATACGGGACTAATCCTAGCCCTAGCCCTACACAAATAT40.28149190710767064No Hit
GTATACGGGCAGTGCTGTATACGGGTCACAGATCCTCTTC30.211118930330753No Hit
GTATACGGGCACATTCGTGAAGGAGAACTAACTCCCACAA30.211118930330753No Hit
GTATACGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.211118930330753No Hit
GTATACGGGCGATTGGTGTTGCTGCTGGTGGTTGGGAGTA30.211118930330753No Hit
GTATACGGGGAGAATCTGTTTGCAAAACTTCTTGATCTTG30.211118930330753No Hit
GTATACGTGTAACAGATAATCTTATGAGATGAGTTTTTGT20.14074595355383532No Hit
GTATACGGGATGCTTAGAAGGAAGTAAATATTCTATATTT20.14074595355383532No Hit
GTATACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT20.14074595355383532No Hit
GTATACGGCTCACAGATCCTCTTCTGAGATGAGTTTTTGT20.14074595355383532No Hit
GTATACGGGATGTCTTAAAAGGCTAAAAGTAGAATCTTCT20.14074595355383532No Hit
GTATACGGGGTGGGGAAGTCTCATGTAACTCAGAACACTA20.14074595355383532No Hit
GTATACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT20.14074595355383532No Hit
GTATACGGGGATAAAATGTCATTTATCTTATTTCTCTATT20.14074595355383532No Hit
GTATACGGGGTTTCAATGGCTTTCTTCCCTTGACCTTTCC20.14074595355383532No Hit
GTATACGGGATCACTGGGCTTTGTATTTCGATGTAACTTC20.14074595355383532No Hit
GTATACGGGTCACAGATACTCTTCTGAAGGAGATTGGGCG20.14074595355383532No Hit
GTATACGGGAAGTGTCCAAAACAGAGCTCTATGAGATCTT20.14074595355383532No Hit
GTATACGGGGGTTTTTATGTTTTAATATTAGGGGAGTAGA20.14074595355383532No Hit
GTATACGGGGCCTTTATTTGATAAGCGATTTGAGATCATT20.14074595355383532No Hit
GTATACGGGATGTTGGTGTGGACTGAACCCCAGAGTGTAT20.14074595355383532No Hit
GTATACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC20.14074595355383532No Hit
GTATACGGGTCACAGATACTCTTCTGAGATGAGTTTTTGT20.14074595355383532No Hit
GTATACGGGGCTGCTTCTGGCACTGCTCCTACCCACGCAG20.14074595355383532No Hit
GTATACGGGGTTTAAATGGCTTTCTTCCCTTGACCTTTCC20.14074595355383532No Hit
GTATACGGGTCACAGATCCCCTTCTGAAGGAGATTGGGCG20.14074595355383532No Hit
GTATACGGGAAAAAAAAAAAACAAAAAAAAAAAAAAAAAA20.14074595355383532No Hit
GTATACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA20.14074595355383532No Hit
GTATACGGGAGTTCTGAAAAAACAAGAACACACACTGGAA20.14074595355383532No Hit
GTATACGGGAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAA20.14074595355383532No Hit
GTATACGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCG20.14074595355383532No Hit
GTATACGGGGAGGGGGCTGTTGGCATTTTGAGAAAGGTTA20.14074595355383532No Hit
GTATACGGGGGACAGTGCCTACGAGGTGATCAAGCTGAAA20.14074595355383532No Hit
GTATACGGGTCACAGATCCTCTTATGAAGGAGATTGGGCG20.14074595355383532No Hit
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG20.14074595355383532No Hit
GTATACGGGGGCAGCGTGTCTCAGTCGGTTGTCGAGTCCC20.14074595355383532No Hit
GTATACGGGTCGTGGTGATCTTGTAATCATGATTTTTATT20.14074595355383532No Hit
GTATACGGGGTTCCACTTGTGGGAAAAGTTTCCGGCAGGG20.14074595355383532No Hit
GTATACGGGAAGCTTATTCATGCTTAAATATGCTTTCAGA20.14074595355383532No Hit
GTATACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTTT20.14074595355383532No Hit
GTATACGGGACTTGTTTAGTTTTTAAACGTTAGTATAATC20.14074595355383532No Hit
GTATACGGGGTACATCTAGGAGCTATAGAACTAGTACCGC20.14074595355383532No Hit
GTATACGGGTCACGGATCCTCTTCTGAAGGAGATTGGGCG20.14074595355383532No Hit
GTATACGGGTGCAGTGCTGTATACGGGATTGAAGTATCAA20.14074595355383532No Hit
GTATACGGGGGGGCTAGCAATAATTTTGTTTAACTTTAAG20.14074595355383532No Hit
GTATACGGGGTAGACTCTGTGTTTGTCTGGAGATAGCCAC20.14074595355383532No Hit
GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG20.14074595355383532No Hit
GTATACGGGGAGCTACCTAAAAACAATTTTATGAATCAAC20.14074595355383532No Hit
GTATACGAGTCACAGATCCTCTTCTGAGATGAGTTTTTGT20.14074595355383532No Hit
GTATACGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGTT20.14074595355383532No Hit
GTATACGGGTCCAAGATCCTCTTCTGAAGGAGATTGGGCG20.14074595355383532No Hit
GTATACGGGATTCTTTCCAACACTGACTAGTTAAACTTAT20.14074595355383532No Hit
GTATACGGGTCAAAGATCCTCTTCTGAGATGAGGATCGGA20.14074595355383532No Hit
GTATACGGGGTCACAGATCCTATTCTGAGATGAGTTTTTG20.14074595355383532No Hit
GTATACGGGCTGTGGTTCGTGGGCATGGACCTGGTACAGG20.14074595355383532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGA402.0472726E-833.9999964
ACGGGGG200.001945374933.9999965
TATACGG1400.032.7857132
GTATACG1400.032.7857131
ATACGGG1350.032.740743
TACGGGG650.031.3846154
ACGGGGA303.4344313E-428.3333325
CGGGGAG250.00581831427.1999996
TACGGGT250.00581831427.1999994
Produced by FastQC (version 0.11.5)