FastQCFastQC Report
Fri 17 Jun 2016
SRR1525834_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525834_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67726
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG15892.3462185866580043No Hit
GAATTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT8981.3259309570918112No Hit
GAATTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC7421.0955910580869976No Hit
GAATTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG3830.5655139828130998No Hit
GAATTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC2600.38389983167468916No Hit
GAATTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG2160.3189321678528187No Hit
GAATTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT1860.2746360334288161No Hit
GAATTAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT1700.2510114284026814No Hit
GAATTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG1680.24805835277441454No Hit
GAATTAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC1670.24658181496028114No Hit
GAATTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG1440.21262144523521248No Hit
GAATTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA1420.20966836960694563No Hit
GAATTAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC1250.1845672267666775No Hit
GAATTAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC1030.15208339485574227No Hit
GAATTAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA980.14470070578507516No Hit
GAATTAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT840.12402917638720727No Hit
GAATTAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT840.12402917638720727No Hit
GAATTAGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG830.12255263857307386No Hit
GAATTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.119599562944807No Hit
GAATTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC810.119599562944807No Hit
GAATTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC800.1181230251306736No Hit
GAATTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.1181230251306736No Hit
GAATTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC790.11664648731654018No Hit
GAATTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT760.11221687387413992No Hit
GAATTAGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT740.10926379824587307No Hit
GAATTAGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG730.10778726043173965No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC730.10778726043173965No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCCT251.9820962E-550.35276839
TCCAATC402.4592737E-950.35276840
CGCATTG600.050.35276439
GCGCATT600.050.35276438
ATGCTCG650.050.27761536
CGGGGCA150.00821485650.2776136
AGCGCTT150.00821485650.2776137
CATCTTT204.0584715E-450.24011615
TGACGAC150.0082391450.24011635
ACATCTT204.0584715E-450.24011614
AGAGCGC150.0082391450.24011635
CAAATCC150.00831231350.12797513
ACCGATC150.0083368150.09070612
ACACATC150.0083368150.09070612
CCGCTCT150.0083368150.09070646
ACAGATT150.0083368150.09070612
ATTATTA150.0083368150.09070646
CGCCCGA150.0083368150.09070647
TCCAAGA204.1186408E-450.09070245
CCACGAT204.1186408E-450.09070246
Produced by FastQC (version 0.11.5)