FastQCFastQC Report
Fri 17 Jun 2016
SRR1525833_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525833_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7505
Sequences flagged as poor quality0
Sequence length40
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC981.3057961359093937No Hit
GAATTAGGGATGAGATGGACTTAAATGAATTGACGGGTAC861.1459027315123251No Hit
GAATTAGGGATGAGATGGACTCAAATGAATTGACGGGTAC771.0259826782145236No Hit
GAATTAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC680.9060626249167222No Hit
GAATTAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC620.8261159227181878No Hit
GAATTAGGGATGAGATGGCCTTAAATGAATTGACGGGTAC570.7594936708860759No Hit
GAATTAGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC380.5063291139240507No Hit
GAATTAGGGATGAGATGGCCTCAAATGAATTGACGGGTAC330.43970686209193866No Hit
GAATTAGGGGAGTGCAGTGCTGAATTAGGGATGAGATGGA290.3864090606262492No Hit
GAATTAGGGAGTGCAGTGCTGAATTAGGGATGAGATGGAC240.31978680879413723No Hit
GAATTAGGGATGAGATGGACTTACATGAATTGACGGGTAC200.2664890073284477No Hit
GAATTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT190.25316455696202533No Hit
GAATTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT160.21319120586275817No Hit
GAATTAGGGATGAGATGGCCTCCAATGAATTGACGGGTAC160.21319120586275817No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC160.21319120586275817No Hit
GAATTAGGGATGAGATGGACTTACAGGAATTGACGGGTAC150.19986675549633579No Hit
GAATTAGGGATGAGATGGACTCACATGAATTGACGGGTAC120.15989340439706862No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG110.14656895403064624No Hit
GAATTAGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC110.14656895403064624No Hit
GAATTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT110.14656895403064624No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC100.13324450366422386No Hit
GAATTAGGGATGAGATGGACTCACAGGAATTGACGGGTAC100.13324450366422386No Hit
GAATTAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGC100.13324450366422386No Hit
GAATTAGGGATGAGATGGACTTAACGGAATTGACGGGTAC90.11992005329780146No Hit
GAATTAGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT90.11992005329780146No Hit
GAATTAGGGGTGCAGTGCTGAATTAGGGATGAGATGGACT90.11992005329780146No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG90.11992005329780146No Hit
GAATTAGGGATGAGATGGCCTTAAAGGACTTGACGGGTAC80.10659560293137908No Hit
GAATTAGGGATGAGATGGCCTCCAAGGAATTGACGGGTAC80.10659560293137908No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG80.10659560293137908No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTAA200.002536970434.2094618
GGACTTA358.6743785E-734.2094617
AGGAATT358.6743785E-734.2094625
GGCCTTA200.002536970434.2094617
GGCCTCA200.002536970434.2094617
AAAGGAA251.7754137E-434.2094623
CCTTAAA200.002536970434.2094619
GGAATTG406.047958E-834.2094626
TAAATGA301.24171875E-534.20945722
AAGGAAT301.24171875E-534.20945724
TTAAATG301.24171875E-534.20945721
ATGGACT650.033.97986615
TGGACTT359.155301E-733.97986616
TGGACTC301.3015824E-533.97986616
TGGCCTT200.002623336233.97986616
TGGCCTC251.8483447E-433.97986616
GACGGGT850.033.97986232
CGGGTAC850.033.97986234
ACGGGTA850.033.97986233
AGGGGGG503.3833203E-1033.7533346
Produced by FastQC (version 0.11.5)